Strain Fitness in Escherichia coli BW25113 around b0043

Experiment: Chlorite 1.25 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfixB and fixC are separated by 50 nucleotidesfixC and fixX overlap by 4 nucleotidesfixX and yaaU are separated by 56 nucleotides b0042: fixB - predicted electron transfer flavoprotein, NAD/FAD-binding domain and ETFP adenine nucleotide-binding domain-like (NCBI), at 43,188 to 44,129 fixB b0043: fixC - predicted oxidoreductase with FAD/NAD(P)-binding domain (NCBI), at 44,180 to 45,466 fixC b0044: fixX - predicted 4Fe-4S ferredoxin-type protein (NCBI), at 45,463 to 45,750 fixX b0045: yaaU - predicted transporter (NCBI), at 45,807 to 47,138 yaaU Position (kb) 44 45 46Strain fitness (log2 ratio) -2 -1 0 1 2at 43.236 kb on - strandat 43.236 kb on - strandat 43.237 kb on + strandat 43.237 kb on + strandat 43.270 kb on - strandat 43.318 kb on - strand, within fixBat 43.432 kb on + strand, within fixBat 43.506 kb on + strand, within fixBat 43.531 kb on + strand, within fixBat 43.539 kb on - strand, within fixBat 43.574 kb on - strand, within fixBat 43.610 kb on + strand, within fixBat 43.630 kb on - strand, within fixBat 43.630 kb on - strand, within fixBat 43.682 kb on - strand, within fixBat 43.692 kb on + strand, within fixBat 43.740 kb on + strand, within fixBat 43.740 kb on + strand, within fixBat 43.835 kb on + strand, within fixBat 43.835 kb on + strand, within fixBat 43.835 kb on - strand, within fixBat 43.838 kb on + strand, within fixBat 43.867 kb on + strand, within fixBat 43.867 kb on + strand, within fixBat 43.871 kb on - strand, within fixBat 43.924 kb on + strand, within fixBat 43.952 kb on + strand, within fixBat 43.970 kb on - strand, within fixBat 44.076 kb on + strandat 44.158 kb on - strandat 44.176 kb on + strandat 44.214 kb on + strandat 44.214 kb on + strandat 44.214 kb on + strandat 44.222 kb on - strandat 44.243 kb on - strandat 44.326 kb on + strand, within fixCat 44.335 kb on - strand, within fixCat 44.335 kb on - strand, within fixCat 44.398 kb on - strand, within fixCat 44.581 kb on - strand, within fixCat 44.622 kb on - strand, within fixCat 44.622 kb on - strand, within fixCat 44.649 kb on + strand, within fixCat 44.675 kb on - strand, within fixCat 44.676 kb on - strand, within fixCat 44.765 kb on + strand, within fixCat 44.857 kb on - strand, within fixCat 44.862 kb on - strand, within fixCat 44.906 kb on - strand, within fixCat 45.034 kb on + strand, within fixCat 45.034 kb on + strand, within fixCat 45.044 kb on + strand, within fixCat 45.044 kb on + strand, within fixCat 45.232 kb on + strand, within fixCat 45.241 kb on - strand, within fixCat 45.323 kb on - strand, within fixCat 45.339 kb on + strandat 45.339 kb on + strandat 45.340 kb on - strandat 45.426 kb on + strandat 45.546 kb on - strand, within fixXat 45.610 kb on + strand, within fixXat 45.700 kb on - strand, within fixXat 45.704 kb on + strand, within fixXat 45.704 kb on + strand, within fixXat 45.844 kb on + strandat 45.880 kb on - strandat 45.924 kb on + strandat 46.113 kb on + strand, within yaaUat 46.113 kb on + strand, within yaaUat 46.142 kb on - strand, within yaaUat 46.144 kb on - strand, within yaaUat 46.176 kb on - strand, within yaaUat 46.176 kb on - strand, within yaaUat 46.240 kb on + strand, within yaaUat 46.284 kb on - strand, within yaaUat 46.329 kb on + strand, within yaaUat 46.329 kb on + strand, within yaaUat 46.463 kb on + strand, within yaaUat 46.463 kb on + strand, within yaaU

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Per-strain Table

Position Strand Gene LocusTag Fraction Chlorite 1.25 mM
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43,236 - +0.0
43,236 - -0.6
43,237 + -0.6
43,237 + +0.7
43,270 - +0.4
43,318 - fixB b0042 0.14 -0.0
43,432 + fixB b0042 0.26 +0.2
43,506 + fixB b0042 0.34 +1.7
43,531 + fixB b0042 0.36 -0.3
43,539 - fixB b0042 0.37 -0.3
43,574 - fixB b0042 0.41 -0.6
43,610 + fixB b0042 0.45 +1.0
43,630 - fixB b0042 0.47 -1.2
43,630 - fixB b0042 0.47 +1.1
43,682 - fixB b0042 0.52 +1.3
43,692 + fixB b0042 0.54 -0.7
43,740 + fixB b0042 0.59 -1.6
43,740 + fixB b0042 0.59 +0.4
43,835 + fixB b0042 0.69 -0.9
43,835 + fixB b0042 0.69 +0.4
43,835 - fixB b0042 0.69 -1.0
43,838 + fixB b0042 0.69 +1.4
43,867 + fixB b0042 0.72 +0.4
43,867 + fixB b0042 0.72 -1.0
43,871 - fixB b0042 0.73 -0.5
43,924 + fixB b0042 0.78 +0.7
43,952 + fixB b0042 0.81 -0.4
43,970 - fixB b0042 0.83 +0.4
44,076 + -1.8
44,158 - +0.4
44,176 + -0.6
44,214 + -2.2
44,214 + -0.6
44,214 + +0.8
44,222 - +0.0
44,243 - +0.7
44,326 + fixC b0043 0.11 +0.2
44,335 - fixC b0043 0.12 -1.4
44,335 - fixC b0043 0.12 -1.2
44,398 - fixC b0043 0.17 -0.6
44,581 - fixC b0043 0.31 -0.3
44,622 - fixC b0043 0.34 -0.6
44,622 - fixC b0043 0.34 -1.6
44,649 + fixC b0043 0.36 +0.4
44,675 - fixC b0043 0.38 -0.4
44,676 - fixC b0043 0.39 +1.4
44,765 + fixC b0043 0.45 -1.6
44,857 - fixC b0043 0.53 -0.8
44,862 - fixC b0043 0.53 +0.9
44,906 - fixC b0043 0.56 -1.8
45,034 + fixC b0043 0.66 -1.2
45,034 + fixC b0043 0.66 +0.4
45,044 + fixC b0043 0.67 +0.4
45,044 + fixC b0043 0.67 -1.4
45,232 + fixC b0043 0.82 +1.2
45,241 - fixC b0043 0.82 +0.4
45,323 - fixC b0043 0.89 -1.2
45,339 + +1.4
45,339 + +0.4
45,340 - -0.3
45,426 + +1.9
45,546 - fixX b0044 0.29 +0.2
45,610 + fixX b0044 0.51 -2.4
45,700 - fixX b0044 0.82 -0.1
45,704 + fixX b0044 0.84 -0.0
45,704 + fixX b0044 0.84 +0.4
45,844 + +0.4
45,880 - +1.6
45,924 + +1.0
46,113 + yaaU b0045 0.23 -0.6
46,113 + yaaU b0045 0.23 +1.1
46,142 - yaaU b0045 0.25 +0.4
46,144 - yaaU b0045 0.25 -2.4
46,176 - yaaU b0045 0.28 +2.4
46,176 - yaaU b0045 0.28 -0.6
46,240 + yaaU b0045 0.33 -1.8
46,284 - yaaU b0045 0.36 +0.9
46,329 + yaaU b0045 0.39 +0.4
46,329 + yaaU b0045 0.39 -0.0
46,463 + yaaU b0045 0.49 +0.4
46,463 + yaaU b0045 0.49 -0.6

Or see this region's nucleotide sequence