Experiment: Chlorite 0.4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nirB and nirD overlap by 4 nucleotides nirD and nirC are separated by 125 nucleotides nirC and cysG are separated by 18 nucleotides
b3365: nirB - nitrite reductase, large subunit, NAD(P)H-binding (NCBI), at 3,492,033 to 3,494,576
nirB
b3366: nirD - nitrite reductase small subunit (NCBI), at 3,494,573 to 3,494,899
nirD
b3367: nirC - nitrite transporter (NCBI), at 3,495,025 to 3,495,831
nirC
b3368: cysG - fused siroheme synthase 1,3-dimethyluroporphyriongen III dehydrogenase and siroheme ferrochelatase/uroporphyrinogen methyltransferase (NCBI), at 3,495,850 to 3,497,223
cysG
Position (kb)
3495
3496 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3494.041 kb on - strand, within nirB at 3494.107 kb on - strand, within nirB at 3494.107 kb on - strand, within nirB at 3494.247 kb on + strand, within nirB at 3494.347 kb on + strand at 3494.395 kb on + strand at 3494.434 kb on + strand at 3494.434 kb on + strand at 3494.452 kb on - strand at 3494.459 kb on + strand at 3494.488 kb on + strand at 3494.499 kb on - strand at 3494.499 kb on - strand at 3494.507 kb on - strand at 3494.507 kb on - strand at 3494.507 kb on - strand at 3494.540 kb on - strand at 3494.548 kb on + strand at 3494.564 kb on - strand at 3494.577 kb on - strand at 3494.577 kb on - strand at 3494.646 kb on - strand, within nirD at 3494.742 kb on - strand, within nirD at 3494.760 kb on + strand, within nirD at 3494.765 kb on + strand, within nirD at 3494.765 kb on + strand, within nirD at 3494.765 kb on + strand, within nirD at 3494.779 kb on - strand, within nirD at 3494.888 kb on - strand at 3494.888 kb on - strand at 3494.939 kb on + strand at 3494.939 kb on + strand at 3494.947 kb on - strand at 3494.947 kb on - strand at 3495.048 kb on + strand at 3495.056 kb on - strand at 3495.062 kb on - strand at 3495.071 kb on + strand at 3495.079 kb on - strand at 3495.113 kb on + strand, within nirC at 3495.113 kb on + strand, within nirC at 3495.123 kb on + strand, within nirC at 3495.124 kb on - strand, within nirC at 3495.124 kb on - strand, within nirC at 3495.129 kb on + strand, within nirC at 3495.129 kb on + strand, within nirC at 3495.131 kb on + strand, within nirC at 3495.148 kb on - strand, within nirC at 3495.148 kb on - strand, within nirC at 3495.148 kb on - strand, within nirC at 3495.148 kb on - strand, within nirC at 3495.182 kb on + strand, within nirC at 3495.195 kb on - strand, within nirC at 3495.197 kb on + strand, within nirC at 3495.197 kb on + strand, within nirC at 3495.251 kb on + strand, within nirC at 3495.251 kb on + strand, within nirC at 3495.251 kb on + strand, within nirC at 3495.259 kb on - strand, within nirC at 3495.271 kb on - strand, within nirC at 3495.308 kb on + strand, within nirC at 3495.308 kb on + strand, within nirC at 3495.311 kb on - strand, within nirC at 3495.311 kb on - strand, within nirC at 3495.353 kb on - strand, within nirC at 3495.358 kb on + strand, within nirC at 3495.388 kb on + strand, within nirC at 3495.388 kb on + strand, within nirC at 3495.388 kb on + strand, within nirC at 3495.388 kb on + strand, within nirC at 3495.388 kb on + strand, within nirC at 3495.396 kb on - strand, within nirC at 3495.491 kb on + strand, within nirC at 3495.535 kb on + strand, within nirC at 3495.579 kb on - strand, within nirC at 3495.609 kb on + strand, within nirC at 3495.623 kb on - strand, within nirC at 3495.623 kb on - strand, within nirC at 3495.626 kb on + strand, within nirC at 3495.626 kb on + strand, within nirC at 3495.645 kb on + strand, within nirC at 3495.645 kb on + strand, within nirC at 3495.653 kb on + strand, within nirC at 3495.654 kb on - strand, within nirC at 3495.678 kb on - strand, within nirC at 3495.678 kb on - strand, within nirC at 3495.702 kb on + strand, within nirC at 3495.705 kb on - strand, within nirC at 3495.710 kb on - strand, within nirC at 3495.715 kb on - strand, within nirC at 3495.717 kb on + strand, within nirC at 3495.744 kb on - strand, within nirC at 3495.832 kb on - strand at 3495.893 kb on + strand at 3495.932 kb on - strand at 3495.936 kb on + strand at 3495.983 kb on + strand at 3495.993 kb on + strand, within cysG at 3496.019 kb on + strand, within cysG at 3496.019 kb on + strand, within cysG at 3496.019 kb on + strand, within cysG at 3496.026 kb on - strand, within cysG at 3496.065 kb on - strand, within cysG at 3496.070 kb on - strand, within cysG at 3496.100 kb on + strand, within cysG at 3496.100 kb on + strand, within cysG at 3496.119 kb on - strand, within cysG at 3496.119 kb on - strand, within cysG at 3496.237 kb on - strand, within cysG at 3496.300 kb on - strand, within cysG at 3496.300 kb on - strand, within cysG at 3496.345 kb on + strand, within cysG at 3496.351 kb on + strand, within cysG at 3496.371 kb on - strand, within cysG at 3496.417 kb on - strand, within cysG at 3496.422 kb on - strand, within cysG at 3496.456 kb on - strand, within cysG at 3496.502 kb on - strand, within cysG at 3496.504 kb on + strand, within cysG at 3496.504 kb on + strand, within cysG at 3496.514 kb on + strand, within cysG at 3496.514 kb on + strand, within cysG at 3496.514 kb on + strand, within cysG at 3496.519 kb on - strand, within cysG at 3496.529 kb on + strand, within cysG at 3496.529 kb on + strand, within cysG at 3496.539 kb on + strand, within cysG at 3496.539 kb on + strand, within cysG at 3496.582 kb on - strand, within cysG at 3496.582 kb on - strand, within cysG at 3496.582 kb on - strand, within cysG at 3496.585 kb on + strand, within cysG at 3496.585 kb on + strand, within cysG at 3496.585 kb on + strand, within cysG at 3496.585 kb on - strand, within cysG at 3496.617 kb on - strand, within cysG at 3496.622 kb on - strand, within cysG at 3496.634 kb on + strand, within cysG at 3496.666 kb on + strand, within cysG at 3496.666 kb on + strand, within cysG at 3496.675 kb on + strand, within cysG at 3496.675 kb on + strand, within cysG at 3496.679 kb on - strand, within cysG at 3496.679 kb on - strand, within cysG at 3496.682 kb on - strand, within cysG at 3496.697 kb on + strand, within cysG at 3496.697 kb on + strand, within cysG at 3496.702 kb on + strand, within cysG at 3496.702 kb on + strand, within cysG at 3496.708 kb on - strand, within cysG at 3496.786 kb on + strand, within cysG at 3496.794 kb on - strand, within cysG at 3496.797 kb on + strand, within cysG at 3496.800 kb on + strand, within cysG at 3496.800 kb on + strand, within cysG at 3496.800 kb on + strand, within cysG at 3496.823 kb on + strand, within cysG at 3496.823 kb on + strand, within cysG at 3496.825 kb on + strand, within cysG
Per-strain Table
Position Strand Gene LocusTag Fraction Chlorite 0.4 mM remove 3,494,041 - nirB b3365 0.79 +1.7 3,494,107 - nirB b3365 0.82 -0.9 3,494,107 - nirB b3365 0.82 -2.7 3,494,247 + nirB b3365 0.87 -1.4 3,494,347 + -4.1 3,494,395 + -1.9 3,494,434 + -1.4 3,494,434 + -1.9 3,494,452 - -1.0 3,494,459 + -4.1 3,494,488 + -1.4 3,494,499 - -1.9 3,494,499 - -1.4 3,494,507 - -1.4 3,494,507 - +1.1 3,494,507 - -2.2 3,494,540 - -1.9 3,494,548 + -1.4 3,494,564 - -0.9 3,494,577 - +0.1 3,494,577 - -0.6 3,494,646 - nirD b3366 0.22 -0.9 3,494,742 - nirD b3366 0.52 -0.7 3,494,760 + nirD b3366 0.57 -3.0 3,494,765 + nirD b3366 0.59 -1.9 3,494,765 + nirD b3366 0.59 -3.0 3,494,765 + nirD b3366 0.59 +0.1 3,494,779 - nirD b3366 0.63 +0.1 3,494,888 - +1.4 3,494,888 - +1.7 3,494,939 + -2.2 3,494,939 + -1.9 3,494,947 - -4.1 3,494,947 - -2.9 3,495,048 + +1.4 3,495,056 - +0.4 3,495,062 - +0.1 3,495,071 + +2.3 3,495,079 - +1.5 3,495,113 + nirC b3367 0.11 -0.2 3,495,113 + nirC b3367 0.11 +1.5 3,495,123 + nirC b3367 0.12 +2.0 3,495,124 - nirC b3367 0.12 +2.1 3,495,124 - nirC b3367 0.12 +1.4 3,495,129 + nirC b3367 0.13 +0.3 3,495,129 + nirC b3367 0.13 +1.6 3,495,131 + nirC b3367 0.13 +1.8 3,495,148 - nirC b3367 0.15 +0.9 3,495,148 - nirC b3367 0.15 +2.6 3,495,148 - nirC b3367 0.15 +1.0 3,495,148 - nirC b3367 0.15 +0.6 3,495,182 + nirC b3367 0.19 +1.8 3,495,195 - nirC b3367 0.21 +0.6 3,495,197 + nirC b3367 0.21 +0.4 3,495,197 + nirC b3367 0.21 +0.3 3,495,251 + nirC b3367 0.28 +1.0 3,495,251 + nirC b3367 0.28 +1.3 3,495,251 + nirC b3367 0.28 -0.0 3,495,259 - nirC b3367 0.29 +1.0 3,495,271 - nirC b3367 0.30 +1.5 3,495,308 + nirC b3367 0.35 +3.6 3,495,308 + nirC b3367 0.35 +0.9 3,495,311 - nirC b3367 0.35 +2.1 3,495,311 - nirC b3367 0.35 +0.8 3,495,353 - nirC b3367 0.41 +1.4 3,495,358 + nirC b3367 0.41 +0.6 3,495,388 + nirC b3367 0.45 +2.3 3,495,388 + nirC b3367 0.45 +1.5 3,495,388 + nirC b3367 0.45 +1.3 3,495,388 + nirC b3367 0.45 +1.0 3,495,388 + nirC b3367 0.45 +1.1 3,495,396 - nirC b3367 0.46 +1.1 3,495,491 + nirC b3367 0.58 +1.4 3,495,535 + nirC b3367 0.63 +1.2 3,495,579 - nirC b3367 0.69 +1.8 3,495,609 + nirC b3367 0.72 -1.4 3,495,623 - nirC b3367 0.74 +2.7 3,495,623 - nirC b3367 0.74 +1.4 3,495,626 + nirC b3367 0.74 +3.0 3,495,626 + nirC b3367 0.74 +1.0 3,495,645 + nirC b3367 0.77 +1.5 3,495,645 + nirC b3367 0.77 +1.7 3,495,653 + nirC b3367 0.78 +2.1 3,495,654 - nirC b3367 0.78 +2.3 3,495,678 - nirC b3367 0.81 +0.1 3,495,678 - nirC b3367 0.81 +1.6 3,495,702 + nirC b3367 0.84 +0.9 3,495,705 - nirC b3367 0.84 +1.4 3,495,710 - nirC b3367 0.85 +1.4 3,495,715 - nirC b3367 0.86 +1.3 3,495,717 + nirC b3367 0.86 +1.5 3,495,744 - nirC b3367 0.89 +1.3 3,495,832 - +1.4 3,495,893 + +0.8 3,495,932 - +1.3 3,495,936 + -0.1 3,495,983 + -0.1 3,495,993 + cysG b3368 0.10 +2.1 3,496,019 + cysG b3368 0.12 -1.3 3,496,019 + cysG b3368 0.12 -0.7 3,496,019 + cysG b3368 0.12 -1.2 3,496,026 - cysG b3368 0.13 +1.2 3,496,065 - cysG b3368 0.16 +0.9 3,496,070 - cysG b3368 0.16 +1.3 3,496,100 + cysG b3368 0.18 -0.2 3,496,100 + cysG b3368 0.18 +0.6 3,496,119 - cysG b3368 0.20 -0.3 3,496,119 - cysG b3368 0.20 +0.1 3,496,237 - cysG b3368 0.28 +1.3 3,496,300 - cysG b3368 0.33 -0.3 3,496,300 - cysG b3368 0.33 +0.7 3,496,345 + cysG b3368 0.36 -1.0 3,496,351 + cysG b3368 0.36 +1.1 3,496,371 - cysG b3368 0.38 -1.6 3,496,417 - cysG b3368 0.41 +1.3 3,496,422 - cysG b3368 0.42 +0.7 3,496,456 - cysG b3368 0.44 +0.1 3,496,502 - cysG b3368 0.47 +0.1 3,496,504 + cysG b3368 0.48 -1.3 3,496,504 + cysG b3368 0.48 +1.0 3,496,514 + cysG b3368 0.48 -0.3 3,496,514 + cysG b3368 0.48 -0.6 3,496,514 + cysG b3368 0.48 +0.1 3,496,519 - cysG b3368 0.49 -0.3 3,496,529 + cysG b3368 0.49 +0.7 3,496,529 + cysG b3368 0.49 -0.4 3,496,539 + cysG b3368 0.50 -0.4 3,496,539 + cysG b3368 0.50 +0.7 3,496,582 - cysG b3368 0.53 +1.4 3,496,582 - cysG b3368 0.53 +1.7 3,496,582 - cysG b3368 0.53 +0.1 3,496,585 + cysG b3368 0.53 -0.2 3,496,585 + cysG b3368 0.53 +0.1 3,496,585 + cysG b3368 0.53 +0.1 3,496,585 - cysG b3368 0.53 +0.1 3,496,617 - cysG b3368 0.56 -1.9 3,496,622 - cysG b3368 0.56 -0.0 3,496,634 + cysG b3368 0.57 -0.7 3,496,666 + cysG b3368 0.59 -1.3 3,496,666 + cysG b3368 0.59 -0.9 3,496,675 + cysG b3368 0.60 +1.7 3,496,675 + cysG b3368 0.60 +0.1 3,496,679 - cysG b3368 0.60 +0.6 3,496,679 - cysG b3368 0.60 -0.2 3,496,682 - cysG b3368 0.61 +0.0 3,496,697 + cysG b3368 0.62 +0.6 3,496,697 + cysG b3368 0.62 +0.9 3,496,702 + cysG b3368 0.62 +0.3 3,496,702 + cysG b3368 0.62 -0.3 3,496,708 - cysG b3368 0.62 +1.1 3,496,786 + cysG b3368 0.68 -0.7 3,496,794 - cysG b3368 0.69 -1.4 3,496,797 + cysG b3368 0.69 -0.6 3,496,800 + cysG b3368 0.69 -0.4 3,496,800 + cysG b3368 0.69 -0.9 3,496,800 + cysG b3368 0.69 +1.1 3,496,823 + cysG b3368 0.71 -2.7 3,496,823 + cysG b3368 0.71 -2.4 3,496,825 + cysG b3368 0.71 -1.4
Or see this region's nucleotide sequence