Strain Fitness in Escherichia coli BW25113 around b2777

Experiment: nitrite 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nt11403176 and 11545539 overlap by 28 nucleotides11545539 and 11687931 overlap by 28 nucleotides11687931 and 11403177 are separated by 0 nucleotides11403177 and 11545540 overlap by 33 nucleotides11545540 and 11687932 overlap by 33 nucleotides11687932 and 11403178 are separated by 0 nucleotides11403178 and 11545541 overlap by 28 nucleotides11545541 and 11687933 overlap by 28 nucleotides11687933 and 11403179 are separated by 0 nucleotides11403179 and 11545542 overlap by 33 nucleotides11545542 and 11687934 overlap by 33 nucleotides11687934 and 11403180 are separated by 0 nucleotides11403180 and 11545543 overlap by 28 nucleotides11545543 and 11687935 overlap by 28 nucleotides11687935 and 11403181 are separated by 0 nucleotides11403181 and 11545544 overlap by 33 nucleotides11545544 and 11687936 overlap by 33 nucleotides11687936 and 11403182 are separated by 0 nucleotides11403182 and 11545545 overlap by 28 nucleotides11545545 and 11687937 overlap by 28 nucleotides11687937 and 11403183 are separated by 0 nucleotides11403183 and 11545546 overlap by 33 nucleotides11545546 and 11687938 overlap by 33 nucleotides11687938 and 11403184 are separated by 0 nucleotides11403184 and 11545547 overlap by 28 nucleotides11545547 and 11687939 overlap by 28 nucleotides11687939 and 11403185 are separated by 0 nucleotides11403185 and 11545548 overlap by 33 nucleotides11545548 and 11687940 overlap by 33 nucleotides11687940 and 11403186 are separated by 0 nucleotides11403186 and 11545549 overlap by 28 nucleotides11545549 and 11687941 overlap by 28 nucleotides11687941 and 11403187 are separated by 0 nucleotides11403187 and 11545550 overlap by 33 nucleotides11545550 and 11687942 overlap by 33 nucleotides11687942 and 11403188 are separated by 0 nucleotides11403188 and 11545551 overlap by 28 nucleotides11545551 and 11687943 overlap by 28 nucleotides11687943 and ygcF are separated by 339 nucleotidesygcF and yqcG are separated by 138 nucleotidesyqcG and ygcG are separated by 13 nucleotides 11403176 - no description, at 2,902,036 to 2,902,063 11403176 11545539 - no description, at 2,902,036 to 2,902,063 11545539 11687931 - no description, at 2,902,036 to 2,902,063 11687931 11403177 - no description, at 2,902,064 to 2,902,096 11403177 11545540 - no description, at 2,902,064 to 2,902,096 11545540 11687932 - no description, at 2,902,064 to 2,902,096 11687932 11403178 - no description, at 2,902,097 to 2,902,124 11403178 11545541 - no description, at 2,902,097 to 2,902,124 11545541 11687933 - no description, at 2,902,097 to 2,902,124 11687933 11403179 - no description, at 2,902,125 to 2,902,157 11403179 11545542 - no description, at 2,902,125 to 2,902,157 11545542 11687934 - no description, at 2,902,125 to 2,902,157 11687934 11403180 - no description, at 2,902,158 to 2,902,185 11403180 11545543 - no description, at 2,902,158 to 2,902,185 11545543 11687935 - no description, at 2,902,158 to 2,902,185 11687935 11403181 - no description, at 2,902,186 to 2,902,218 11403181 11545544 - no description, at 2,902,186 to 2,902,218 11545544 11687936 - no description, at 2,902,186 to 2,902,218 11687936 11403182 - no description, at 2,902,219 to 2,902,246 11403182 11545545 - no description, at 2,902,219 to 2,902,246 11545545 11687937 - no description, at 2,902,219 to 2,902,246 11687937 11403183 - no description, at 2,902,247 to 2,902,279 11403183 11545546 - no description, at 2,902,247 to 2,902,279 11545546 11687938 - no description, at 2,902,247 to 2,902,279 11687938 11403184 - no description, at 2,902,280 to 2,902,307 11403184 11545547 - no description, at 2,902,280 to 2,902,307 11545547 11687939 - no description, at 2,902,280 to 2,902,307 11687939 11403185 - no description, at 2,902,308 to 2,902,340 11403185 11545548 - no description, at 2,902,308 to 2,902,340 11545548 11687940 - no description, at 2,902,308 to 2,902,340 11687940 11403186 - no description, at 2,902,341 to 2,902,368 11403186 11545549 - no description, at 2,902,341 to 2,902,368 11545549 11687941 - no description, at 2,902,341 to 2,902,368 11687941 11403187 - no description, at 2,902,369 to 2,902,401 11403187 11545550 - no description, at 2,902,369 to 2,902,401 11545550 11687942 - no description, at 2,902,369 to 2,902,401 11687942 11403188 - no description, at 2,902,402 to 2,902,429 11403188 11545551 - no description, at 2,902,402 to 2,902,429 11545551 11687943 - no description, at 2,902,402 to 2,902,429 11687943 b2777: ygcF - hypothetical protein (NCBI), at 2,902,769 to 2,903,440 ygcF b4682: yqcG - expressed protein (RefSeq), at 2,903,579 to 2,903,719 yqcG b2778: ygcG - orf, hypothetical protein (VIMSS), at 2,903,733 to 2,904,605 ygcG Position (kb) 2902 2903 2904Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2901.926 kb on - strandat 2901.941 kb on - strandat 2901.941 kb on - strandat 2901.946 kb on + strandat 2901.967 kb on + strandat 2901.967 kb on + strandat 2901.968 kb on + strandat 2902.034 kb on - strandat 2902.058 kb on - strandat 2902.058 kb on - strandat 2902.058 kb on - strandat 2902.071 kb on - strandat 2902.071 kb on - strandat 2902.076 kb on - strandat 2902.076 kb on - strandat 2902.076 kb on - strandat 2902.076 kb on - strandat 2902.076 kb on - strandat 2902.080 kb on - strandat 2902.082 kb on - strandat 2902.085 kb on - strandat 2902.085 kb on - strandat 2902.087 kb on - strandat 2902.087 kb on - strandat 2902.087 kb on - strandat 2902.087 kb on - strandat 2902.099 kb on - strandat 2902.100 kb on - strandat 2902.114 kb on - strandat 2902.114 kb on - strandat 2902.180 kb on + strandat 2902.195 kb on + strandat 2902.205 kb on - strandat 2902.205 kb on - strandat 2902.205 kb on - strandat 2902.224 kb on + strandat 2902.228 kb on - strandat 2902.244 kb on - strandat 2902.250 kb on + strandat 2902.250 kb on + strandat 2902.252 kb on + strandat 2902.254 kb on + strandat 2902.256 kb on - strandat 2902.260 kb on - strandat 2902.265 kb on + strandat 2902.273 kb on - strandat 2902.367 kb on + strandat 2902.479 kb on - strandat 2902.488 kb on + strandat 2902.488 kb on + strandat 2902.523 kb on + strandat 2902.523 kb on + strandat 2902.735 kb on - strandat 2902.746 kb on + strandat 2902.770 kb on - strandat 2902.770 kb on - strandat 2902.797 kb on - strandat 2902.817 kb on - strandat 2902.832 kb on - strandat 2902.832 kb on - strandat 2902.846 kb on - strand, within ygcFat 2902.852 kb on - strand, within ygcFat 2902.852 kb on - strand, within ygcFat 2902.859 kb on - strand, within ygcFat 2902.876 kb on + strand, within ygcFat 2902.882 kb on - strand, within ygcFat 2902.887 kb on + strand, within ygcFat 2902.887 kb on - strand, within ygcFat 2902.887 kb on - strand, within ygcFat 2902.887 kb on - strand, within ygcFat 2902.887 kb on - strand, within ygcFat 2902.917 kb on - strand, within ygcFat 2902.917 kb on - strand, within ygcFat 2902.950 kb on - strand, within ygcFat 2902.981 kb on + strand, within ygcFat 2902.990 kb on - strand, within ygcFat 2902.990 kb on - strand, within ygcFat 2903.002 kb on + strand, within ygcFat 2903.010 kb on - strand, within ygcFat 2903.010 kb on - strand, within ygcFat 2903.015 kb on + strand, within ygcFat 2903.034 kb on - strand, within ygcFat 2903.050 kb on - strand, within ygcFat 2903.050 kb on - strand, within ygcFat 2903.058 kb on + strand, within ygcFat 2903.058 kb on + strand, within ygcFat 2903.074 kb on - strand, within ygcFat 2903.074 kb on - strand, within ygcFat 2903.187 kb on + strand, within ygcFat 2903.235 kb on + strand, within ygcFat 2903.307 kb on + strand, within ygcFat 2903.328 kb on + strand, within ygcFat 2903.332 kb on + strand, within ygcFat 2903.332 kb on + strand, within ygcFat 2903.335 kb on - strand, within ygcFat 2903.340 kb on - strand, within ygcFat 2903.388 kb on + strandat 2903.388 kb on + strandat 2903.394 kb on + strandat 2903.394 kb on + strandat 2903.396 kb on + strandat 2903.409 kb on + strandat 2903.416 kb on - strandat 2903.417 kb on - strandat 2903.417 kb on - strandat 2903.417 kb on - strandat 2903.437 kb on + strandat 2903.445 kb on - strandat 2903.448 kb on + strandat 2903.471 kb on - strandat 2903.503 kb on + strandat 2903.510 kb on + strandat 2903.525 kb on + strandat 2904.015 kb on - strand, within ygcGat 2904.015 kb on - strand, within ygcGat 2904.017 kb on + strand, within ygcGat 2904.017 kb on + strand, within ygcGat 2904.017 kb on - strand, within ygcGat 2904.027 kb on - strand, within ygcGat 2904.045 kb on + strand, within ygcGat 2904.061 kb on + strand, within ygcGat 2904.107 kb on + strand, within ygcGat 2904.113 kb on + strand, within ygcGat 2904.167 kb on + strand, within ygcGat 2904.205 kb on + strand, within ygcGat 2904.209 kb on + strand, within ygcGat 2904.439 kb on - strand, within ygcG

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrite 20 mM
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2,901,926 - +0.2
2,901,941 - +1.0
2,901,941 - +0.8
2,901,946 + +0.4
2,901,967 + -1.0
2,901,967 + +0.0
2,901,968 + -0.2
2,902,034 - -0.4
2,902,058 - +0.0
2,902,058 - +0.3
2,902,058 - +1.0
2,902,071 - +0.4
2,902,071 - -2.0
2,902,076 - +0.1
2,902,076 - +1.4
2,902,076 - -0.7
2,902,076 - +1.4
2,902,076 - +0.0
2,902,080 - +0.3
2,902,082 - -0.2
2,902,085 - +0.8
2,902,085 - -1.6
2,902,087 - +1.4
2,902,087 - +0.2
2,902,087 - +0.4
2,902,087 - -0.6
2,902,099 - -0.8
2,902,100 - +0.2
2,902,114 - +0.4
2,902,114 - +1.4
2,902,180 + -0.6
2,902,195 + +1.4
2,902,205 - -0.3
2,902,205 - +0.5
2,902,205 - +0.8
2,902,224 + -0.5
2,902,228 - +1.3
2,902,244 - +0.6
2,902,250 + +0.4
2,902,250 + +0.2
2,902,252 + +0.0
2,902,254 + +0.0
2,902,256 - +0.8
2,902,260 - +1.4
2,902,265 + +0.5
2,902,273 - +0.8
2,902,367 + +0.0
2,902,479 - +3.0
2,902,488 + -0.6
2,902,488 + +1.4
2,902,523 + +0.0
2,902,523 + +1.4
2,902,735 - -1.0
2,902,746 + +0.0
2,902,770 - +0.0
2,902,770 - +0.4
2,902,797 - +0.8
2,902,817 - +0.6
2,902,832 - +0.6
2,902,832 - +0.6
2,902,846 - ygcF b2777 0.11 +1.5
2,902,852 - ygcF b2777 0.12 +0.0
2,902,852 - ygcF b2777 0.12 -0.4
2,902,859 - ygcF b2777 0.13 +0.9
2,902,876 + ygcF b2777 0.16 +1.1
2,902,882 - ygcF b2777 0.17 +0.4
2,902,887 + ygcF b2777 0.18 +1.0
2,902,887 - ygcF b2777 0.18 -0.2
2,902,887 - ygcF b2777 0.18 +0.3
2,902,887 - ygcF b2777 0.18 +0.9
2,902,887 - ygcF b2777 0.18 -1.6
2,902,917 - ygcF b2777 0.22 -0.6
2,902,917 - ygcF b2777 0.22 +0.0
2,902,950 - ygcF b2777 0.27 -0.1
2,902,981 + ygcF b2777 0.32 +0.8
2,902,990 - ygcF b2777 0.33 +0.1
2,902,990 - ygcF b2777 0.33 +0.3
2,903,002 + ygcF b2777 0.35 +1.3
2,903,010 - ygcF b2777 0.36 -2.1
2,903,010 - ygcF b2777 0.36 +0.0
2,903,015 + ygcF b2777 0.37 +0.9
2,903,034 - ygcF b2777 0.39 +1.4
2,903,050 - ygcF b2777 0.42 +1.0
2,903,050 - ygcF b2777 0.42 +0.6
2,903,058 + ygcF b2777 0.43 +0.0
2,903,058 + ygcF b2777 0.43 +0.8
2,903,074 - ygcF b2777 0.45 +1.4
2,903,074 - ygcF b2777 0.45 +1.4
2,903,187 + ygcF b2777 0.62 +1.6
2,903,235 + ygcF b2777 0.69 +0.7
2,903,307 + ygcF b2777 0.80 +0.9
2,903,328 + ygcF b2777 0.83 +1.6
2,903,332 + ygcF b2777 0.84 +0.6
2,903,332 + ygcF b2777 0.84 +0.0
2,903,335 - ygcF b2777 0.84 +0.3
2,903,340 - ygcF b2777 0.85 +1.0
2,903,388 + -1.6
2,903,388 + +0.4
2,903,394 + +0.1
2,903,394 + +1.0
2,903,396 + +0.8
2,903,409 + +0.3
2,903,416 - +0.6
2,903,417 - +0.4
2,903,417 - +2.0
2,903,417 - +0.8
2,903,437 + +0.5
2,903,445 - +0.2
2,903,448 + +0.3
2,903,471 - +1.0
2,903,503 + +2.0
2,903,510 + +2.8
2,903,525 + +0.4
2,904,015 - ygcG b2778 0.32 -1.1
2,904,015 - ygcG b2778 0.32 -0.7
2,904,017 + ygcG b2778 0.33 +0.6
2,904,017 + ygcG b2778 0.33 -1.5
2,904,017 - ygcG b2778 0.33 -2.8
2,904,027 - ygcG b2778 0.34 -1.0
2,904,045 + ygcG b2778 0.36 -1.2
2,904,061 + ygcG b2778 0.38 -0.4
2,904,107 + ygcG b2778 0.43 +1.4
2,904,113 + ygcG b2778 0.44 +2.0
2,904,167 + ygcG b2778 0.50 +1.0
2,904,205 + ygcG b2778 0.54 +0.6
2,904,209 + ygcG b2778 0.55 +0.4
2,904,439 - ygcG b2778 0.81 -0.1

Or see this region's nucleotide sequence