Experiment: nitrite 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt flgI and flgJ overlap by 1 nucleotides flgJ and flgK are separated by 65 nucleotides
b1080: flgI - homolog of Salmonella P-ring of flagella basal body (VIMSS), at 1,135,497 to 1,136,594
flgI
b1081: flgJ - flagellar biosynthesis protein FlgJ (NCBI), at 1,136,594 to 1,137,535
flgJ
b1082: flgK - flagellar hook-associated protein K (NCBI), at 1,137,601 to 1,139,244
flgK
Position (kb)
1136
1137
1138 Strain fitness (log2 ratio)
-1
0
1
2 at 1135.634 kb on - strand, within flgI at 1135.838 kb on + strand, within flgI at 1135.849 kb on + strand, within flgI at 1135.867 kb on - strand, within flgI at 1135.913 kb on - strand, within flgI at 1136.103 kb on + strand, within flgI at 1136.129 kb on - strand, within flgI at 1136.129 kb on - strand, within flgI at 1136.165 kb on - strand, within flgI at 1136.168 kb on + strand, within flgI at 1136.312 kb on - strand, within flgI at 1136.312 kb on - strand, within flgI at 1136.319 kb on + strand, within flgI at 1136.335 kb on - strand, within flgI at 1136.399 kb on + strand, within flgI at 1136.413 kb on - strand, within flgI at 1136.451 kb on + strand, within flgI at 1136.507 kb on + strand at 1136.507 kb on + strand at 1136.620 kb on - strand at 1136.622 kb on - strand at 1136.622 kb on - strand at 1136.776 kb on + strand, within flgJ at 1136.776 kb on + strand, within flgJ at 1136.810 kb on + strand, within flgJ at 1136.810 kb on + strand, within flgJ at 1136.821 kb on + strand, within flgJ at 1136.854 kb on - strand, within flgJ at 1136.867 kb on - strand, within flgJ at 1136.957 kb on - strand, within flgJ at 1137.015 kb on - strand, within flgJ at 1137.015 kb on - strand, within flgJ at 1137.122 kb on - strand, within flgJ at 1137.373 kb on - strand, within flgJ at 1137.452 kb on + strand at 1137.684 kb on + strand at 1137.691 kb on - strand at 1137.691 kb on - strand at 1137.705 kb on - strand at 1137.740 kb on - strand at 1138.112 kb on - strand, within flgK at 1138.255 kb on + strand, within flgK at 1138.306 kb on + strand, within flgK at 1138.350 kb on + strand, within flgK at 1138.362 kb on + strand, within flgK at 1138.369 kb on + strand, within flgK at 1138.422 kb on + strand, within flgK at 1138.422 kb on + strand, within flgK at 1138.469 kb on + strand, within flgK at 1138.530 kb on - strand, within flgK
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite 20 mM remove 1,135,634 - flgI b1080 0.12 -0.1 1,135,838 + flgI b1080 0.31 +0.7 1,135,849 + flgI b1080 0.32 +1.1 1,135,867 - flgI b1080 0.34 +1.1 1,135,913 - flgI b1080 0.38 +0.4 1,136,103 + flgI b1080 0.55 -0.0 1,136,129 - flgI b1080 0.58 -0.2 1,136,129 - flgI b1080 0.58 -0.2 1,136,165 - flgI b1080 0.61 +2.1 1,136,168 + flgI b1080 0.61 +0.8 1,136,312 - flgI b1080 0.74 -0.0 1,136,312 - flgI b1080 0.74 -1.0 1,136,319 + flgI b1080 0.75 +0.3 1,136,335 - flgI b1080 0.76 +1.2 1,136,399 + flgI b1080 0.82 +0.6 1,136,413 - flgI b1080 0.83 -0.7 1,136,451 + flgI b1080 0.87 +0.3 1,136,507 + +0.4 1,136,507 + +0.6 1,136,620 - -0.2 1,136,622 - -0.8 1,136,622 - -0.1 1,136,776 + flgJ b1081 0.19 +0.9 1,136,776 + flgJ b1081 0.19 +1.2 1,136,810 + flgJ b1081 0.23 +0.6 1,136,810 + flgJ b1081 0.23 +0.6 1,136,821 + flgJ b1081 0.24 +1.1 1,136,854 - flgJ b1081 0.28 +0.3 1,136,867 - flgJ b1081 0.29 +0.9 1,136,957 - flgJ b1081 0.39 +1.0 1,137,015 - flgJ b1081 0.45 -0.2 1,137,015 - flgJ b1081 0.45 +0.5 1,137,122 - flgJ b1081 0.56 -0.1 1,137,373 - flgJ b1081 0.83 +1.9 1,137,452 + -1.6 1,137,684 + -0.8 1,137,691 - +1.1 1,137,691 - +1.1 1,137,705 - +0.0 1,137,740 - -0.2 1,138,112 - flgK b1082 0.31 +0.1 1,138,255 + flgK b1082 0.40 -0.2 1,138,306 + flgK b1082 0.43 +0.1 1,138,350 + flgK b1082 0.46 -0.5 1,138,362 + flgK b1082 0.46 +0.5 1,138,369 + flgK b1082 0.47 -1.5 1,138,422 + flgK b1082 0.50 -0.2 1,138,422 + flgK b1082 0.50 -0.5 1,138,469 + flgK b1082 0.53 +1.0 1,138,530 - flgK b1082 0.57 +0.7
Or see this region's nucleotide sequence