Strain Fitness in Escherichia coli BW25113 around b0871

Experiment: nitrite 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntltaE and poxB are separated by 36 nucleotidespoxB and hcr are separated by 132 nucleotides b0870: ltaE - L-allo-threonine aldolase, PLP-dependent (NCBI), at 907,516 to 908,517 ltaE b0871: poxB - pyruvate dehydrogenase (NCBI), at 908,554 to 910,272 poxB b0872: hcr - HCP oxidoreductase, NADH-dependent (NCBI), at 910,405 to 911,373 hcr Position (kb) 908 909 910 911Strain fitness (log2 ratio) -2 -1 0 1 2 3at 908.109 kb on + strand, within ltaEat 908.179 kb on - strand, within ltaEat 908.192 kb on - strand, within ltaEat 908.220 kb on + strand, within ltaEat 908.235 kb on + strand, within ltaEat 908.314 kb on + strand, within ltaEat 908.337 kb on + strand, within ltaEat 908.403 kb on - strand, within ltaEat 908.461 kb on - strandat 908.493 kb on + strandat 908.493 kb on + strandat 908.513 kb on + strandat 908.626 kb on + strandat 908.626 kb on + strandat 908.794 kb on + strand, within poxBat 908.819 kb on + strand, within poxBat 908.939 kb on + strand, within poxBat 909.025 kb on - strand, within poxBat 909.025 kb on - strand, within poxBat 909.079 kb on - strandat 909.134 kb on + strand, within poxBat 909.142 kb on + strand, within poxBat 909.183 kb on + strand, within poxBat 909.183 kb on + strand, within poxBat 909.465 kb on - strand, within poxBat 909.465 kb on - strand, within poxBat 909.579 kb on + strand, within poxBat 909.587 kb on - strand, within poxBat 909.647 kb on + strand, within poxBat 909.726 kb on - strand, within poxBat 909.748 kb on + strand, within poxBat 909.756 kb on - strand, within poxBat 909.848 kb on - strand, within poxBat 909.850 kb on - strand, within poxBat 909.868 kb on - strand, within poxBat 909.874 kb on + strand, within poxBat 909.874 kb on + strand, within poxBat 909.880 kb on - strand, within poxBat 910.173 kb on + strandat 910.173 kb on + strandat 910.181 kb on - strandat 910.181 kb on - strandat 910.214 kb on + strandat 910.215 kb on + strandat 910.215 kb on + strandat 910.292 kb on - strandat 910.293 kb on + strandat 910.343 kb on - strandat 910.452 kb on - strandat 910.452 kb on - strandat 910.738 kb on + strand, within hcrat 910.816 kb on - strand, within hcrat 910.826 kb on - strandat 911.125 kb on - strand, within hcrat 911.167 kb on - strand, within hcr

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrite 20 mM
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908,109 + ltaE b0870 0.59 +2.5
908,179 - ltaE b0870 0.66 +1.2
908,192 - ltaE b0870 0.67 +2.8
908,220 + ltaE b0870 0.70 +0.6
908,235 + ltaE b0870 0.72 +0.2
908,314 + ltaE b0870 0.80 +0.3
908,337 + ltaE b0870 0.82 -0.9
908,403 - ltaE b0870 0.89 -1.0
908,461 - +0.8
908,493 + +0.1
908,493 + +0.8
908,513 + +1.3
908,626 + +0.2
908,626 + -0.2
908,794 + poxB b0871 0.14 +0.6
908,819 + poxB b0871 0.15 +0.8
908,939 + poxB b0871 0.22 -0.2
909,025 - poxB b0871 0.27 +1.0
909,025 - poxB b0871 0.27 +1.0
909,079 - -0.2
909,134 + poxB b0871 0.34 +2.1
909,142 + poxB b0871 0.34 +0.3
909,183 + poxB b0871 0.37 +0.9
909,183 + poxB b0871 0.37 -0.4
909,465 - poxB b0871 0.53 +1.8
909,465 - poxB b0871 0.53 +0.6
909,579 + poxB b0871 0.60 +1.2
909,587 - poxB b0871 0.60 -1.2
909,647 + poxB b0871 0.64 +1.1
909,726 - poxB b0871 0.68 +0.8
909,748 + poxB b0871 0.69 +0.1
909,756 - poxB b0871 0.70 +1.1
909,848 - poxB b0871 0.75 +0.8
909,850 - poxB b0871 0.75 +0.8
909,868 - poxB b0871 0.76 -0.2
909,874 + poxB b0871 0.77 +0.1
909,874 + poxB b0871 0.77 +1.8
909,880 - poxB b0871 0.77 +0.2
910,173 + -0.7
910,173 + +0.8
910,181 - -0.2
910,181 - +0.8
910,214 + +0.1
910,215 + -1.8
910,215 + +0.4
910,292 - -0.2
910,293 + +0.6
910,343 - -0.2
910,452 - -0.6
910,452 - +0.2
910,738 + hcr b0872 0.34 -2.5
910,816 - hcr b0872 0.42 -0.8
910,826 - -0.2
911,125 - hcr b0872 0.74 -0.9
911,167 - hcr b0872 0.79 +1.2

Or see this region's nucleotide sequence