Experiment: nitrite 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yjiD and yjiE overlap by 8 nucleotides yjiE and iadA are separated by 64 nucleotides iadA and yjiG are separated by 12 nucleotides yjiG and yjiH overlap by 4 nucleotides
b4326: yjiD - orf, hypothetical protein (VIMSS), at 4,555,016 to 4,555,408
yjiD
b4327: yjiE - predicted DNA-binding transcriptional regulator (NCBI), at 4,555,401 to 4,556,312
yjiE
b4328: iadA - isoaspartyl dipeptidase (NCBI), at 4,556,377 to 4,557,549
iadA
b4329: yjiG - conserved inner membrane protein (NCBI), at 4,557,562 to 4,558,023
yjiG
b4330: yjiH - orf, hypothetical protein (VIMSS), at 4,558,020 to 4,558,703
yjiH
Position (kb)
4556
4557
4558 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4555.398 kb on - strand at 4555.407 kb on - strand at 4555.415 kb on + strand at 4555.415 kb on + strand at 4555.498 kb on + strand at 4555.498 kb on + strand, within yjiE at 4555.667 kb on + strand, within yjiE at 4555.731 kb on + strand, within yjiE at 4555.739 kb on - strand at 4555.764 kb on - strand, within yjiE at 4555.764 kb on - strand, within yjiE at 4555.791 kb on - strand, within yjiE at 4555.976 kb on + strand, within yjiE at 4556.042 kb on - strand, within yjiE at 4556.106 kb on + strand, within yjiE at 4556.168 kb on + strand, within yjiE at 4556.168 kb on + strand, within yjiE at 4556.334 kb on - strand at 4556.382 kb on - strand at 4556.382 kb on - strand at 4556.416 kb on - strand at 4556.449 kb on - strand at 4556.449 kb on - strand at 4556.463 kb on + strand at 4556.526 kb on + strand, within iadA at 4556.552 kb on - strand, within iadA at 4556.556 kb on - strand, within iadA at 4556.568 kb on - strand, within iadA at 4556.596 kb on + strand, within iadA at 4556.596 kb on + strand, within iadA at 4556.602 kb on + strand, within iadA at 4556.644 kb on + strand, within iadA at 4556.908 kb on + strand, within iadA at 4556.942 kb on + strand, within iadA at 4556.953 kb on - strand, within iadA at 4556.953 kb on - strand, within iadA at 4556.955 kb on + strand, within iadA at 4556.982 kb on - strand, within iadA at 4557.054 kb on - strand, within iadA at 4557.054 kb on - strand, within iadA at 4557.073 kb on + strand, within iadA at 4557.120 kb on - strand, within iadA at 4557.138 kb on + strand, within iadA at 4557.246 kb on - strand, within iadA at 4557.249 kb on + strand, within iadA at 4557.257 kb on - strand, within iadA at 4557.509 kb on + strand at 4557.623 kb on - strand, within yjiG at 4557.665 kb on - strand, within yjiG at 4557.665 kb on - strand, within yjiG at 4557.667 kb on - strand, within yjiG at 4557.793 kb on + strand, within yjiG at 4557.793 kb on + strand, within yjiG at 4557.801 kb on - strand, within yjiG at 4557.801 kb on - strand, within yjiG at 4557.837 kb on - strand, within yjiG at 4557.837 kb on - strand, within yjiG at 4558.050 kb on - strand at 4558.104 kb on - strand, within yjiH at 4558.116 kb on + strand, within yjiH at 4558.181 kb on - strand, within yjiH at 4558.185 kb on + strand, within yjiH at 4558.211 kb on - strand, within yjiH at 4558.301 kb on + strand, within yjiH at 4558.328 kb on + strand, within yjiH at 4558.392 kb on + strand, within yjiH at 4558.392 kb on + strand, within yjiH at 4558.428 kb on + strand, within yjiH
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite 10 mM remove 4,555,398 - +0.1 4,555,407 - +0.3 4,555,415 + +0.5 4,555,415 + +0.0 4,555,498 + +0.0 4,555,498 + yjiE b4327 0.11 +1.4 4,555,667 + yjiE b4327 0.29 +0.2 4,555,731 + yjiE b4327 0.36 +0.4 4,555,739 - +0.0 4,555,764 - yjiE b4327 0.40 +1.3 4,555,764 - yjiE b4327 0.40 +0.0 4,555,791 - yjiE b4327 0.43 -0.4 4,555,976 + yjiE b4327 0.63 -0.5 4,556,042 - yjiE b4327 0.70 +0.7 4,556,106 + yjiE b4327 0.77 +0.5 4,556,168 + yjiE b4327 0.84 -0.3 4,556,168 + yjiE b4327 0.84 -0.4 4,556,334 - +0.1 4,556,382 - -1.1 4,556,382 - +0.2 4,556,416 - +0.6 4,556,449 - +0.0 4,556,449 - +0.8 4,556,463 + +0.7 4,556,526 + iadA b4328 0.13 -1.1 4,556,552 - iadA b4328 0.15 -0.1 4,556,556 - iadA b4328 0.15 -1.0 4,556,568 - iadA b4328 0.16 -0.9 4,556,596 + iadA b4328 0.19 +1.6 4,556,596 + iadA b4328 0.19 +2.4 4,556,602 + iadA b4328 0.19 +1.3 4,556,644 + iadA b4328 0.23 +1.6 4,556,908 + iadA b4328 0.45 +1.0 4,556,942 + iadA b4328 0.48 -0.2 4,556,953 - iadA b4328 0.49 +0.8 4,556,953 - iadA b4328 0.49 +0.0 4,556,955 + iadA b4328 0.49 -0.4 4,556,982 - iadA b4328 0.52 -3.1 4,557,054 - iadA b4328 0.58 -1.0 4,557,054 - iadA b4328 0.58 -1.9 4,557,073 + iadA b4328 0.59 +0.0 4,557,120 - iadA b4328 0.63 -0.2 4,557,138 + iadA b4328 0.65 -0.5 4,557,246 - iadA b4328 0.74 -0.2 4,557,249 + iadA b4328 0.74 +0.0 4,557,257 - iadA b4328 0.75 +0.0 4,557,509 + +0.4 4,557,623 - yjiG b4329 0.13 +0.8 4,557,665 - yjiG b4329 0.22 +0.4 4,557,665 - yjiG b4329 0.22 +0.4 4,557,667 - yjiG b4329 0.23 -0.2 4,557,793 + yjiG b4329 0.50 +0.3 4,557,793 + yjiG b4329 0.50 -0.6 4,557,801 - yjiG b4329 0.52 -0.3 4,557,801 - yjiG b4329 0.52 +0.0 4,557,837 - yjiG b4329 0.60 -0.4 4,557,837 - yjiG b4329 0.60 +0.1 4,558,050 - +0.5 4,558,104 - yjiH b4330 0.12 -0.2 4,558,116 + yjiH b4330 0.14 +0.4 4,558,181 - yjiH b4330 0.24 +0.9 4,558,185 + yjiH b4330 0.24 +1.4 4,558,211 - yjiH b4330 0.28 -1.5 4,558,301 + yjiH b4330 0.41 +0.3 4,558,328 + yjiH b4330 0.45 +0.3 4,558,392 + yjiH b4330 0.54 +0.8 4,558,392 + yjiH b4330 0.54 -0.8 4,558,428 + yjiH b4330 0.60 -1.2
Or see this region's nucleotide sequence