Experiment: trans-aconitic (C) 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFR28_01111 and PFR28_01112 are separated by 98 nucleotides PFR28_01112 and PFR28_01113 are separated by 115 nucleotides PFR28_01113 and PFR28_01114 are separated by 190 nucleotides PFR28_01114 and PFR28_01115 are separated by 106 nucleotides
PFR28_01111: PFR28_01111 - 3-ketoacyl-CoA thiolase, at 1,228,715 to 1,229,890
_01111
PFR28_01112: PFR28_01112 - hypothetical protein, at 1,229,989 to 1,230,225
_01112
PFR28_01113: PFR28_01113 - DNA topoisomerase 1, at 1,230,341 to 1,232,953
_01113
PFR28_01114: PFR28_01114 - hypothetical protein, at 1,233,144 to 1,233,668
_01114
PFR28_01115: PFR28_01115 - hypothetical protein, at 1,233,775 to 1,234,008
_01115
Position (kb) 1230
1231
1232
1233 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 1229.343 kb on - strand, within PFR28_01111 at 1229.570 kb on + strand, within PFR28_01111 at 1229.788 kb on + strand at 1229.889 kb on - strand at 1229.889 kb on - strand at 1229.957 kb on + strand at 1229.997 kb on - strand at 1229.997 kb on - strand at 1230.010 kb on + strand at 1230.019 kb on + strand, within PFR28_01112 at 1230.019 kb on + strand, within PFR28_01112 at 1230.019 kb on + strand, within PFR28_01112 at 1230.019 kb on + strand, within PFR28_01112 at 1230.019 kb on + strand, within PFR28_01112 at 1230.019 kb on + strand, within PFR28_01112 at 1230.019 kb on + strand, within PFR28_01112 at 1230.019 kb on + strand, within PFR28_01112 at 1230.020 kb on - strand, within PFR28_01112 at 1230.020 kb on - strand, within PFR28_01112 at 1230.020 kb on - strand, within PFR28_01112 at 1230.020 kb on - strand, within PFR28_01112 at 1230.020 kb on - strand, within PFR28_01112 at 1230.020 kb on - strand, within PFR28_01112 at 1230.020 kb on - strand, within PFR28_01112 at 1230.020 kb on - strand, within PFR28_01112 at 1230.041 kb on + strand, within PFR28_01112 at 1230.042 kb on - strand, within PFR28_01112 at 1230.088 kb on + strand, within PFR28_01112 at 1230.089 kb on - strand, within PFR28_01112 at 1230.089 kb on - strand, within PFR28_01112 at 1230.089 kb on - strand, within PFR28_01112 at 1230.101 kb on - strand, within PFR28_01112 at 1230.272 kb on + strand at 1230.272 kb on + strand at 1230.272 kb on + strand at 1230.272 kb on + strand at 1230.272 kb on + strand at 1230.272 kb on + strand at 1230.272 kb on + strand at 1230.272 kb on + strand at 1230.272 kb on + strand at 1230.272 kb on + strand at 1230.272 kb on + strand at 1230.274 kb on + strand at 1230.274 kb on + strand at 1230.274 kb on + strand at 1230.274 kb on + strand at 1230.274 kb on + strand at 1230.274 kb on + strand at 1230.274 kb on + strand at 1230.274 kb on + strand at 1230.274 kb on + strand at 1230.274 kb on + strand at 1230.274 kb on + strand at 1230.274 kb on + strand at 1230.274 kb on + strand at 1230.274 kb on + strand at 1230.274 kb on + strand at 1230.274 kb on + strand at 1230.274 kb on + strand at 1230.274 kb on + strand at 1230.274 kb on + strand at 1230.274 kb on + strand at 1230.274 kb on + strand at 1230.274 kb on + strand at 1230.274 kb on + strand at 1230.274 kb on + strand at 1230.274 kb on + strand at 1230.274 kb on + strand at 1230.274 kb on + strand at 1230.274 kb on + strand at 1230.274 kb on + strand at 1230.274 kb on + strand at 1230.274 kb on + strand at 1230.274 kb on + strand at 1230.274 kb on + strand at 1230.276 kb on + strand at 1230.276 kb on + strand at 1230.276 kb on + strand at 1230.276 kb on + strand at 1230.276 kb on + strand at 1230.276 kb on + strand at 1230.276 kb on + strand at 1230.276 kb on + strand at 1230.276 kb on + strand at 1230.276 kb on + strand at 1230.276 kb on + strand at 1230.276 kb on + strand at 1230.276 kb on + strand at 1230.276 kb on + strand at 1230.276 kb on + strand at 1230.276 kb on + strand at 1230.276 kb on + strand at 1230.276 kb on + strand at 1230.276 kb on + strand at 1230.276 kb on + strand at 1230.276 kb on + strand at 1230.276 kb on + strand at 1230.276 kb on + strand at 1230.276 kb on + strand at 1230.278 kb on + strand at 1230.278 kb on + strand at 1230.278 kb on + strand at 1232.660 kb on + strand, within PFR28_01113 at 1232.660 kb on + strand, within PFR28_01113 at 1232.660 kb on + strand, within PFR28_01113 at 1232.660 kb on + strand, within PFR28_01113 at 1232.660 kb on + strand, within PFR28_01113 at 1232.660 kb on + strand, within PFR28_01113 at 1232.660 kb on + strand, within PFR28_01113 at 1232.660 kb on + strand, within PFR28_01113 at 1232.660 kb on + strand, within PFR28_01113 at 1232.660 kb on + strand, within PFR28_01113 at 1232.660 kb on + strand, within PFR28_01113 at 1232.661 kb on - strand, within PFR28_01113 at 1232.661 kb on - strand, within PFR28_01113 at 1232.661 kb on - strand, within PFR28_01113 at 1232.661 kb on - strand, within PFR28_01113 at 1232.661 kb on - strand, within PFR28_01113 at 1232.783 kb on + strand at 1232.783 kb on + strand at 1232.784 kb on - strand at 1232.784 kb on - strand at 1232.867 kb on + strand at 1232.912 kb on + strand at 1233.159 kb on + strand at 1233.160 kb on - strand at 1233.160 kb on - strand at 1233.291 kb on + strand, within PFR28_01114 at 1233.291 kb on + strand, within PFR28_01114 at 1233.292 kb on - strand, within PFR28_01114 at 1233.292 kb on - strand, within PFR28_01114 at 1233.333 kb on + strand, within PFR28_01114 at 1233.334 kb on - strand, within PFR28_01114 at 1233.334 kb on - strand, within PFR28_01114 at 1233.334 kb on - strand, within PFR28_01114 at 1233.529 kb on + strand, within PFR28_01114 at 1233.529 kb on + strand, within PFR28_01114 at 1233.530 kb on - strand, within PFR28_01114 at 1233.530 kb on - strand, within PFR28_01114 at 1233.530 kb on - strand, within PFR28_01114 at 1233.530 kb on - strand, within PFR28_01114 at 1233.741 kb on + strand at 1233.742 kb on - strand at 1233.742 kb on - strand at 1233.742 kb on - strand at 1233.743 kb on + strand at 1233.743 kb on + strand at 1233.743 kb on + strand at 1233.743 kb on + strand at 1233.743 kb on + strand at 1233.743 kb on + strand at 1233.743 kb on + strand at 1233.743 kb on + strand at 1233.743 kb on + strand at 1233.743 kb on + strand at 1233.743 kb on + strand at 1233.744 kb on - strand at 1233.744 kb on - strand at 1233.744 kb on - strand at 1233.744 kb on - strand at 1233.744 kb on - strand at 1233.774 kb on + strand at 1233.774 kb on + strand at 1233.774 kb on + strand at 1233.775 kb on - strand at 1233.775 kb on - strand at 1233.780 kb on + strand at 1233.780 kb on + strand at 1233.780 kb on + strand at 1233.780 kb on + strand at 1233.780 kb on + strand at 1233.780 kb on + strand at 1233.781 kb on - strand at 1233.781 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction trans-aconitic (C) 5 mM remove 1,229,343 - PFR28_01111 0.53 -1.4 1,229,570 + PFR28_01111 0.73 -0.0 1,229,788 + +0.7 1,229,889 - +0.4 1,229,889 - +1.6 1,229,957 + -1.1 1,229,997 - +0.4 1,229,997 - +0.3 1,230,010 + +0.1 1,230,019 + PFR28_01112 0.13 +0.6 1,230,019 + PFR28_01112 0.13 +0.0 1,230,019 + PFR28_01112 0.13 -2.1 1,230,019 + PFR28_01112 0.13 -0.0 1,230,019 + PFR28_01112 0.13 -0.4 1,230,019 + PFR28_01112 0.13 +0.0 1,230,019 + PFR28_01112 0.13 -1.4 1,230,019 + PFR28_01112 0.13 -0.7 1,230,020 - PFR28_01112 0.13 +0.3 1,230,020 - PFR28_01112 0.13 +0.5 1,230,020 - PFR28_01112 0.13 +1.1 1,230,020 - PFR28_01112 0.13 -0.0 1,230,020 - PFR28_01112 0.13 -0.1 1,230,020 - PFR28_01112 0.13 +0.1 1,230,020 - PFR28_01112 0.13 -0.2 1,230,020 - PFR28_01112 0.13 -1.0 1,230,041 + PFR28_01112 0.22 -0.9 1,230,042 - PFR28_01112 0.22 -0.9 1,230,088 + PFR28_01112 0.42 +0.9 1,230,089 - PFR28_01112 0.42 +0.9 1,230,089 - PFR28_01112 0.42 -0.3 1,230,089 - PFR28_01112 0.42 -1.0 1,230,101 - PFR28_01112 0.47 +1.3 1,230,272 + -0.7 1,230,272 + +0.0 1,230,272 + +0.2 1,230,272 + +1.5 1,230,272 + -0.7 1,230,272 + +0.6 1,230,272 + +0.5 1,230,272 + -0.4 1,230,272 + -0.9 1,230,272 + +0.6 1,230,272 + -0.4 1,230,274 + +0.6 1,230,274 + -1.0 1,230,274 + +0.1 1,230,274 + +0.9 1,230,274 + -0.3 1,230,274 + +0.3 1,230,274 + +0.7 1,230,274 + -0.4 1,230,274 + -2.5 1,230,274 + +1.1 1,230,274 + -0.8 1,230,274 + +0.9 1,230,274 + +1.0 1,230,274 + +0.3 1,230,274 + -0.1 1,230,274 + +0.8 1,230,274 + +0.3 1,230,274 + -0.1 1,230,274 + -2.0 1,230,274 + +1.8 1,230,274 + +0.1 1,230,274 + -0.1 1,230,274 + +0.3 1,230,274 + -0.2 1,230,274 + +0.7 1,230,274 + -0.4 1,230,274 + +0.0 1,230,274 + +0.9 1,230,274 + +0.7 1,230,274 + +0.4 1,230,274 + -1.0 1,230,274 + -0.1 1,230,274 + +0.3 1,230,276 + +0.5 1,230,276 + +0.0 1,230,276 + -0.1 1,230,276 + -0.2 1,230,276 + +0.6 1,230,276 + -0.3 1,230,276 + +0.3 1,230,276 + -0.0 1,230,276 + -0.2 1,230,276 + +0.3 1,230,276 + +0.1 1,230,276 + +0.0 1,230,276 + +0.1 1,230,276 + +0.3 1,230,276 + +1.3 1,230,276 + +0.1 1,230,276 + +0.2 1,230,276 + +1.1 1,230,276 + -0.1 1,230,276 + +0.7 1,230,276 + -0.1 1,230,276 + -0.4 1,230,276 + -0.4 1,230,276 + +0.4 1,230,278 + +0.8 1,230,278 + +0.6 1,230,278 + -0.3 1,232,660 + PFR28_01113 0.89 -1.1 1,232,660 + PFR28_01113 0.89 -2.1 1,232,660 + PFR28_01113 0.89 -2.0 1,232,660 + PFR28_01113 0.89 -0.5 1,232,660 + PFR28_01113 0.89 -3.3 1,232,660 + PFR28_01113 0.89 -1.1 1,232,660 + PFR28_01113 0.89 +0.2 1,232,660 + PFR28_01113 0.89 -1.4 1,232,660 + PFR28_01113 0.89 -0.6 1,232,660 + PFR28_01113 0.89 -1.1 1,232,660 + PFR28_01113 0.89 -2.5 1,232,661 - PFR28_01113 0.89 -1.6 1,232,661 - PFR28_01113 0.89 -0.6 1,232,661 - PFR28_01113 0.89 -0.6 1,232,661 - PFR28_01113 0.89 -1.5 1,232,661 - PFR28_01113 0.89 -1.4 1,232,783 + -0.9 1,232,783 + -0.3 1,232,784 - -0.9 1,232,784 - +1.4 1,232,867 + +1.4 1,232,912 + +1.4 1,233,159 + -3.8 1,233,160 - -3.1 1,233,160 - -2.9 1,233,291 + PFR28_01114 0.28 -2.8 1,233,291 + PFR28_01114 0.28 -1.1 1,233,292 - PFR28_01114 0.28 -2.0 1,233,292 - PFR28_01114 0.28 -1.9 1,233,333 + PFR28_01114 0.36 -1.7 1,233,334 - PFR28_01114 0.36 -2.1 1,233,334 - PFR28_01114 0.36 -1.3 1,233,334 - PFR28_01114 0.36 -2.3 1,233,529 + PFR28_01114 0.73 -0.9 1,233,529 + PFR28_01114 0.73 -2.1 1,233,530 - PFR28_01114 0.74 -2.0 1,233,530 - PFR28_01114 0.74 -0.1 1,233,530 - PFR28_01114 0.74 -2.0 1,233,530 - PFR28_01114 0.74 -2.7 1,233,741 + -0.1 1,233,742 - +0.1 1,233,742 - +1.4 1,233,742 - -3.0 1,233,743 + -1.3 1,233,743 + -0.3 1,233,743 + +0.7 1,233,743 + +1.2 1,233,743 + +1.7 1,233,743 + +1.3 1,233,743 + +0.3 1,233,743 + -0.7 1,233,743 + -0.3 1,233,743 + +0.4 1,233,743 + +0.3 1,233,744 - -1.5 1,233,744 - -0.2 1,233,744 - -0.3 1,233,744 - -0.1 1,233,744 - +1.2 1,233,774 + +0.9 1,233,774 + -0.7 1,233,774 + +0.1 1,233,775 - +0.1 1,233,775 - +0.4 1,233,780 + -0.4 1,233,780 + +0.7 1,233,780 + +0.1 1,233,780 + -0.5 1,233,780 + +0.3 1,233,780 + +2.0 1,233,781 - +0.7 1,233,781 - -0.1
Or see this region's nucleotide sequence