Experiment: trans-aconitic (C) 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFR28_00682 and PFR28_00683 are separated by 232 nucleotides PFR28_00683 and PFR28_00684 overlap by 8 nucleotides PFR28_00684 and PFR28_00685 are separated by 85 nucleotides PFR28_00685 and PFR28_00686 are separated by 35 nucleotides
PFR28_00682: PFR28_00682 - hypothetical protein, at 725,942 to 726,127
_00682
PFR28_00683: PFR28_00683 - Transcriptional regulatory protein tctD, at 726,360 to 727,031
_00683
PFR28_00684: PFR28_00684 - Sensor protein QseC, at 727,024 to 728,415
_00684
PFR28_00685: PFR28_00685 - hypothetical protein, at 728,501 to 729,322
_00685
PFR28_00686: PFR28_00686 - tRNA-modifying protein YgfZ, at 729,358 to 730,299
_00686
Position (kb) 727
728
729 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 726.024 kb on + strand, within PFR28_00682 at 726.024 kb on + strand, within PFR28_00682 at 726.024 kb on + strand, within PFR28_00682 at 726.025 kb on - strand, within PFR28_00682 at 726.025 kb on - strand, within PFR28_00682 at 726.025 kb on - strand, within PFR28_00682 at 726.025 kb on - strand, within PFR28_00682 at 726.134 kb on + strand at 726.134 kb on + strand at 726.134 kb on + strand at 726.134 kb on + strand at 726.135 kb on - strand at 726.135 kb on - strand at 726.144 kb on + strand at 726.145 kb on - strand at 726.145 kb on - strand at 726.145 kb on - strand at 726.153 kb on + strand at 726.217 kb on - strand at 726.217 kb on - strand at 726.232 kb on + strand at 726.232 kb on + strand at 726.252 kb on - strand at 726.355 kb on + strand at 726.355 kb on + strand at 726.355 kb on + strand at 726.356 kb on - strand at 726.356 kb on - strand at 726.356 kb on - strand at 726.356 kb on - strand at 726.492 kb on + strand, within PFR28_00683 at 726.493 kb on - strand, within PFR28_00683 at 726.514 kb on + strand, within PFR28_00683 at 726.515 kb on - strand, within PFR28_00683 at 726.515 kb on - strand, within PFR28_00683 at 726.760 kb on - strand, within PFR28_00683 at 726.760 kb on - strand, within PFR28_00683 at 726.760 kb on - strand, within PFR28_00683 at 726.760 kb on - strand, within PFR28_00683 at 726.760 kb on - strand, within PFR28_00683 at 726.760 kb on - strand, within PFR28_00683 at 726.937 kb on - strand, within PFR28_00683 at 726.937 kb on - strand, within PFR28_00683 at 726.937 kb on - strand, within PFR28_00683 at 727.006 kb on - strand at 727.029 kb on + strand at 727.029 kb on + strand at 727.030 kb on - strand at 727.030 kb on - strand at 727.030 kb on - strand at 727.030 kb on - strand at 727.030 kb on - strand at 727.214 kb on + strand, within PFR28_00684 at 727.215 kb on - strand, within PFR28_00684 at 727.231 kb on + strand, within PFR28_00684 at 727.231 kb on + strand, within PFR28_00684 at 727.231 kb on + strand, within PFR28_00684 at 727.231 kb on + strand, within PFR28_00684 at 727.231 kb on + strand, within PFR28_00684 at 727.231 kb on + strand, within PFR28_00684 at 727.231 kb on + strand, within PFR28_00684 at 727.231 kb on + strand, within PFR28_00684 at 727.231 kb on + strand, within PFR28_00684 at 727.231 kb on + strand, within PFR28_00684 at 727.231 kb on + strand, within PFR28_00684 at 727.231 kb on + strand, within PFR28_00684 at 727.231 kb on + strand, within PFR28_00684 at 727.231 kb on + strand, within PFR28_00684 at 727.231 kb on + strand, within PFR28_00684 at 727.231 kb on + strand, within PFR28_00684 at 727.232 kb on - strand, within PFR28_00684 at 727.232 kb on - strand, within PFR28_00684 at 727.232 kb on - strand, within PFR28_00684 at 727.232 kb on - strand, within PFR28_00684 at 727.232 kb on - strand, within PFR28_00684 at 727.232 kb on - strand, within PFR28_00684 at 727.232 kb on - strand, within PFR28_00684 at 727.232 kb on - strand, within PFR28_00684 at 727.232 kb on - strand, within PFR28_00684 at 727.232 kb on - strand, within PFR28_00684 at 727.232 kb on - strand, within PFR28_00684 at 727.232 kb on - strand, within PFR28_00684 at 727.232 kb on - strand, within PFR28_00684 at 727.232 kb on - strand, within PFR28_00684 at 727.232 kb on - strand, within PFR28_00684 at 727.232 kb on - strand, within PFR28_00684 at 727.276 kb on + strand, within PFR28_00684 at 727.276 kb on + strand, within PFR28_00684 at 727.276 kb on + strand, within PFR28_00684 at 727.276 kb on + strand, within PFR28_00684 at 727.276 kb on + strand, within PFR28_00684 at 727.276 kb on + strand, within PFR28_00684 at 727.276 kb on + strand, within PFR28_00684 at 727.276 kb on + strand, within PFR28_00684 at 727.276 kb on + strand, within PFR28_00684 at 727.276 kb on + strand, within PFR28_00684 at 727.276 kb on + strand, within PFR28_00684 at 727.277 kb on - strand, within PFR28_00684 at 727.277 kb on - strand, within PFR28_00684 at 727.277 kb on - strand, within PFR28_00684 at 727.277 kb on - strand, within PFR28_00684 at 727.277 kb on - strand, within PFR28_00684 at 727.277 kb on - strand, within PFR28_00684 at 727.277 kb on - strand, within PFR28_00684 at 727.277 kb on - strand, within PFR28_00684 at 727.277 kb on - strand, within PFR28_00684 at 727.277 kb on - strand, within PFR28_00684 at 727.277 kb on - strand, within PFR28_00684 at 727.277 kb on - strand, within PFR28_00684 at 727.277 kb on - strand, within PFR28_00684 at 727.277 kb on - strand, within PFR28_00684 at 727.277 kb on - strand, within PFR28_00684 at 727.277 kb on - strand, within PFR28_00684 at 727.277 kb on - strand, within PFR28_00684 at 727.277 kb on - strand, within PFR28_00684 at 727.279 kb on + strand, within PFR28_00684 at 727.279 kb on + strand, within PFR28_00684 at 727.280 kb on - strand, within PFR28_00684 at 727.387 kb on + strand, within PFR28_00684 at 727.388 kb on - strand, within PFR28_00684 at 727.388 kb on - strand, within PFR28_00684 at 727.388 kb on - strand, within PFR28_00684 at 727.399 kb on + strand, within PFR28_00684 at 727.399 kb on + strand, within PFR28_00684 at 727.399 kb on + strand, within PFR28_00684 at 727.400 kb on - strand, within PFR28_00684 at 727.400 kb on - strand, within PFR28_00684 at 727.400 kb on - strand, within PFR28_00684 at 727.400 kb on - strand, within PFR28_00684 at 727.694 kb on + strand, within PFR28_00684 at 727.694 kb on + strand, within PFR28_00684 at 727.695 kb on - strand, within PFR28_00684 at 727.695 kb on - strand, within PFR28_00684 at 727.695 kb on - strand, within PFR28_00684 at 728.151 kb on + strand, within PFR28_00684 at 728.151 kb on + strand, within PFR28_00684 at 728.151 kb on + strand, within PFR28_00684 at 728.152 kb on - strand, within PFR28_00684 at 728.267 kb on - strand, within PFR28_00684 at 728.427 kb on + strand at 728.427 kb on + strand at 728.427 kb on + strand at 728.427 kb on + strand at 728.428 kb on - strand at 728.428 kb on - strand at 728.428 kb on - strand at 728.428 kb on - strand at 728.428 kb on - strand at 728.428 kb on - strand at 728.446 kb on + strand at 728.446 kb on + strand at 728.446 kb on + strand at 728.446 kb on + strand at 728.446 kb on + strand at 728.446 kb on + strand at 728.446 kb on + strand at 728.446 kb on + strand at 728.446 kb on + strand at 728.446 kb on + strand at 728.446 kb on + strand at 728.446 kb on + strand at 728.446 kb on + strand at 728.446 kb on + strand at 728.446 kb on + strand at 728.446 kb on + strand at 728.446 kb on + strand at 728.446 kb on + strand at 728.446 kb on + strand at 728.447 kb on - strand at 728.447 kb on - strand at 728.447 kb on - strand at 728.447 kb on - strand at 728.447 kb on - strand at 728.447 kb on - strand at 728.447 kb on - strand at 728.447 kb on - strand at 728.447 kb on - strand at 728.447 kb on - strand at 728.447 kb on - strand at 728.447 kb on - strand at 728.447 kb on - strand at 728.447 kb on - strand at 728.447 kb on - strand at 728.448 kb on + strand at 728.448 kb on + strand at 728.448 kb on + strand at 728.448 kb on + strand at 728.448 kb on + strand at 728.448 kb on + strand at 728.448 kb on + strand at 728.448 kb on + strand at 728.448 kb on + strand at 728.448 kb on + strand at 728.448 kb on + strand at 728.449 kb on - strand at 728.449 kb on - strand at 728.449 kb on - strand at 728.449 kb on - strand at 728.449 kb on - strand at 728.449 kb on - strand at 728.449 kb on - strand at 728.449 kb on - strand at 728.449 kb on - strand at 728.449 kb on - strand at 728.449 kb on - strand at 728.449 kb on - strand at 728.449 kb on - strand at 728.449 kb on - strand at 728.449 kb on - strand at 728.449 kb on - strand at 728.449 kb on - strand at 728.449 kb on - strand at 728.505 kb on + strand at 728.505 kb on + strand at 728.505 kb on + strand at 728.506 kb on - strand at 728.506 kb on - strand at 728.506 kb on - strand at 728.631 kb on + strand, within PFR28_00685 at 728.631 kb on + strand, within PFR28_00685 at 728.631 kb on + strand, within PFR28_00685 at 728.631 kb on + strand, within PFR28_00685 at 728.631 kb on + strand, within PFR28_00685 at 728.631 kb on + strand, within PFR28_00685 at 728.637 kb on + strand, within PFR28_00685 at 728.638 kb on - strand, within PFR28_00685 at 728.638 kb on - strand, within PFR28_00685 at 728.638 kb on - strand, within PFR28_00685 at 728.638 kb on - strand, within PFR28_00685 at 728.667 kb on + strand, within PFR28_00685 at 728.668 kb on - strand, within PFR28_00685 at 728.668 kb on - strand, within PFR28_00685 at 728.841 kb on + strand, within PFR28_00685 at 728.841 kb on + strand, within PFR28_00685 at 728.841 kb on + strand, within PFR28_00685 at 728.841 kb on + strand, within PFR28_00685 at 728.841 kb on + strand, within PFR28_00685 at 728.842 kb on - strand, within PFR28_00685 at 728.842 kb on - strand, within PFR28_00685 at 728.842 kb on - strand, within PFR28_00685 at 728.916 kb on + strand, within PFR28_00685 at 728.934 kb on + strand, within PFR28_00685 at 728.934 kb on + strand, within PFR28_00685 at 728.934 kb on + strand, within PFR28_00685 at 728.934 kb on + strand, within PFR28_00685 at 728.935 kb on - strand, within PFR28_00685 at 728.935 kb on - strand, within PFR28_00685 at 729.054 kb on + strand, within PFR28_00685 at 729.054 kb on + strand, within PFR28_00685 at 729.054 kb on + strand, within PFR28_00685 at 729.054 kb on + strand, within PFR28_00685 at 729.054 kb on + strand, within PFR28_00685 at 729.054 kb on + strand, within PFR28_00685 at 729.054 kb on + strand, within PFR28_00685 at 729.054 kb on + strand, within PFR28_00685 at 729.054 kb on + strand, within PFR28_00685 at 729.054 kb on + strand, within PFR28_00685 at 729.054 kb on + strand, within PFR28_00685 at 729.055 kb on - strand, within PFR28_00685 at 729.055 kb on - strand, within PFR28_00685 at 729.055 kb on - strand, within PFR28_00685 at 729.055 kb on - strand, within PFR28_00685 at 729.055 kb on - strand, within PFR28_00685 at 729.305 kb on - strand at 729.333 kb on + strand at 729.392 kb on + strand at 729.393 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction trans-aconitic (C) 5 mM remove 726,024 + PFR28_00682 0.44 -1.5 726,024 + PFR28_00682 0.44 -1.0 726,024 + PFR28_00682 0.44 -0.5 726,025 - PFR28_00682 0.45 +0.1 726,025 - PFR28_00682 0.45 +0.2 726,025 - PFR28_00682 0.45 +1.9 726,025 - PFR28_00682 0.45 +0.5 726,134 + -1.3 726,134 + -0.5 726,134 + -1.0 726,134 + -0.6 726,135 - -0.2 726,135 - -0.1 726,144 + -0.5 726,145 - -0.2 726,145 - +0.3 726,145 - -3.0 726,153 + -2.0 726,217 - -0.3 726,217 - +0.2 726,232 + -2.1 726,232 + -2.1 726,252 - -1.2 726,355 + -0.0 726,355 + -0.0 726,355 + -0.5 726,356 - -1.7 726,356 - -0.5 726,356 - -1.1 726,356 - +0.8 726,492 + PFR28_00683 0.20 -0.3 726,493 - PFR28_00683 0.20 -0.8 726,514 + PFR28_00683 0.23 -1.5 726,515 - PFR28_00683 0.23 -0.4 726,515 - PFR28_00683 0.23 +0.7 726,760 - PFR28_00683 0.60 -1.7 726,760 - PFR28_00683 0.60 -0.5 726,760 - PFR28_00683 0.60 +0.6 726,760 - PFR28_00683 0.60 +0.8 726,760 - PFR28_00683 0.60 -1.9 726,760 - PFR28_00683 0.60 -2.8 726,937 - PFR28_00683 0.86 -0.4 726,937 - PFR28_00683 0.86 -1.0 726,937 - PFR28_00683 0.86 -0.3 727,006 - -1.8 727,029 + +0.5 727,029 + +0.7 727,030 - -0.6 727,030 - -1.3 727,030 - -0.8 727,030 - -1.5 727,030 - -3.0 727,214 + PFR28_00684 0.14 +0.1 727,215 - PFR28_00684 0.14 -0.7 727,231 + PFR28_00684 0.15 -2.4 727,231 + PFR28_00684 0.15 +0.1 727,231 + PFR28_00684 0.15 -0.1 727,231 + PFR28_00684 0.15 -0.9 727,231 + PFR28_00684 0.15 -0.1 727,231 + PFR28_00684 0.15 -0.2 727,231 + PFR28_00684 0.15 -0.3 727,231 + PFR28_00684 0.15 -0.3 727,231 + PFR28_00684 0.15 -0.2 727,231 + PFR28_00684 0.15 -0.8 727,231 + PFR28_00684 0.15 -1.0 727,231 + PFR28_00684 0.15 -0.6 727,231 + PFR28_00684 0.15 -1.2 727,231 + PFR28_00684 0.15 -1.0 727,231 + PFR28_00684 0.15 -0.3 727,231 + PFR28_00684 0.15 -0.3 727,232 - PFR28_00684 0.15 -2.1 727,232 - PFR28_00684 0.15 -0.2 727,232 - PFR28_00684 0.15 +0.0 727,232 - PFR28_00684 0.15 +0.7 727,232 - PFR28_00684 0.15 -2.0 727,232 - PFR28_00684 0.15 -0.5 727,232 - PFR28_00684 0.15 -1.1 727,232 - PFR28_00684 0.15 -0.7 727,232 - PFR28_00684 0.15 -0.2 727,232 - PFR28_00684 0.15 +0.3 727,232 - PFR28_00684 0.15 -1.5 727,232 - PFR28_00684 0.15 -0.8 727,232 - PFR28_00684 0.15 -2.3 727,232 - PFR28_00684 0.15 -0.6 727,232 - PFR28_00684 0.15 -1.3 727,232 - PFR28_00684 0.15 -2.1 727,276 + PFR28_00684 0.18 -1.3 727,276 + PFR28_00684 0.18 -0.8 727,276 + PFR28_00684 0.18 -0.5 727,276 + PFR28_00684 0.18 -0.1 727,276 + PFR28_00684 0.18 -1.9 727,276 + PFR28_00684 0.18 -1.7 727,276 + PFR28_00684 0.18 -0.8 727,276 + PFR28_00684 0.18 +0.4 727,276 + PFR28_00684 0.18 -2.9 727,276 + PFR28_00684 0.18 -1.3 727,276 + PFR28_00684 0.18 -0.1 727,277 - PFR28_00684 0.18 -0.8 727,277 - PFR28_00684 0.18 -0.3 727,277 - PFR28_00684 0.18 -1.1 727,277 - PFR28_00684 0.18 -2.9 727,277 - PFR28_00684 0.18 -0.6 727,277 - PFR28_00684 0.18 +0.1 727,277 - PFR28_00684 0.18 -0.2 727,277 - PFR28_00684 0.18 -1.4 727,277 - PFR28_00684 0.18 -0.1 727,277 - PFR28_00684 0.18 -1.5 727,277 - PFR28_00684 0.18 -1.3 727,277 - PFR28_00684 0.18 -0.3 727,277 - PFR28_00684 0.18 -1.2 727,277 - PFR28_00684 0.18 -0.8 727,277 - PFR28_00684 0.18 -0.7 727,277 - PFR28_00684 0.18 -0.3 727,277 - PFR28_00684 0.18 -1.1 727,277 - PFR28_00684 0.18 -0.3 727,279 + PFR28_00684 0.18 -1.4 727,279 + PFR28_00684 0.18 -0.7 727,280 - PFR28_00684 0.18 -1.3 727,387 + PFR28_00684 0.26 -1.4 727,388 - PFR28_00684 0.26 -0.8 727,388 - PFR28_00684 0.26 -0.2 727,388 - PFR28_00684 0.26 -1.8 727,399 + PFR28_00684 0.27 +0.0 727,399 + PFR28_00684 0.27 -1.8 727,399 + PFR28_00684 0.27 -1.6 727,400 - PFR28_00684 0.27 -1.7 727,400 - PFR28_00684 0.27 +0.1 727,400 - PFR28_00684 0.27 +0.3 727,400 - PFR28_00684 0.27 -1.8 727,694 + PFR28_00684 0.48 -0.9 727,694 + PFR28_00684 0.48 +0.2 727,695 - PFR28_00684 0.48 -1.3 727,695 - PFR28_00684 0.48 -0.9 727,695 - PFR28_00684 0.48 +0.5 728,151 + PFR28_00684 0.81 +0.9 728,151 + PFR28_00684 0.81 -0.8 728,151 + PFR28_00684 0.81 +0.1 728,152 - PFR28_00684 0.81 +1.5 728,267 - PFR28_00684 0.89 -0.0 728,427 + +0.6 728,427 + -0.6 728,427 + +0.4 728,427 + +0.2 728,428 - +0.1 728,428 - +0.5 728,428 - +1.0 728,428 - -0.2 728,428 - -0.1 728,428 - +0.8 728,446 + +0.9 728,446 + +0.7 728,446 + -0.2 728,446 + -0.2 728,446 + -0.8 728,446 + -0.9 728,446 + +0.9 728,446 + -1.5 728,446 + -0.3 728,446 + -0.4 728,446 + +0.2 728,446 + +0.1 728,446 + -0.6 728,446 + +2.5 728,446 + +0.3 728,446 + -0.2 728,446 + -0.7 728,446 + +0.4 728,446 + +0.1 728,447 - -1.6 728,447 - +0.6 728,447 - +0.0 728,447 - +0.4 728,447 - -0.2 728,447 - +1.5 728,447 - -0.1 728,447 - +0.9 728,447 - +0.0 728,447 - -1.2 728,447 - -0.4 728,447 - -0.7 728,447 - -0.3 728,447 - +0.6 728,447 - -1.0 728,448 + -0.9 728,448 + -0.1 728,448 + +0.3 728,448 + -0.8 728,448 + -1.1 728,448 + -0.3 728,448 + +0.6 728,448 + +0.9 728,448 + -0.1 728,448 + +0.0 728,448 + -0.8 728,449 - -0.5 728,449 - +1.1 728,449 - +0.0 728,449 - +0.5 728,449 - +0.9 728,449 - +0.6 728,449 - +0.1 728,449 - +1.3 728,449 - -0.2 728,449 - +0.1 728,449 - -0.6 728,449 - -0.2 728,449 - +0.4 728,449 - +0.8 728,449 - +0.6 728,449 - +0.2 728,449 - -0.2 728,449 - -0.5 728,505 + +0.2 728,505 + +0.7 728,505 + -0.0 728,506 - +1.8 728,506 - -0.2 728,506 - +0.4 728,631 + PFR28_00685 0.16 -1.2 728,631 + PFR28_00685 0.16 -0.8 728,631 + PFR28_00685 0.16 -0.0 728,631 + PFR28_00685 0.16 -0.1 728,631 + PFR28_00685 0.16 -0.1 728,631 + PFR28_00685 0.16 +1.0 728,637 + PFR28_00685 0.17 -0.2 728,638 - PFR28_00685 0.17 +1.2 728,638 - PFR28_00685 0.17 +0.5 728,638 - PFR28_00685 0.17 -1.1 728,638 - PFR28_00685 0.17 +1.3 728,667 + PFR28_00685 0.20 -0.3 728,668 - PFR28_00685 0.20 -0.1 728,668 - PFR28_00685 0.20 -0.2 728,841 + PFR28_00685 0.41 -0.5 728,841 + PFR28_00685 0.41 -1.6 728,841 + PFR28_00685 0.41 +0.3 728,841 + PFR28_00685 0.41 +0.3 728,841 + PFR28_00685 0.41 -0.8 728,842 - PFR28_00685 0.41 -0.5 728,842 - PFR28_00685 0.41 +0.5 728,842 - PFR28_00685 0.41 -0.9 728,916 + PFR28_00685 0.50 -1.2 728,934 + PFR28_00685 0.53 -0.2 728,934 + PFR28_00685 0.53 +0.1 728,934 + PFR28_00685 0.53 -0.2 728,934 + PFR28_00685 0.53 -0.8 728,935 - PFR28_00685 0.53 -0.8 728,935 - PFR28_00685 0.53 +0.5 729,054 + PFR28_00685 0.67 -0.5 729,054 + PFR28_00685 0.67 +0.7 729,054 + PFR28_00685 0.67 +0.7 729,054 + PFR28_00685 0.67 -0.7 729,054 + PFR28_00685 0.67 +0.2 729,054 + PFR28_00685 0.67 +0.3 729,054 + PFR28_00685 0.67 +0.2 729,054 + PFR28_00685 0.67 -0.3 729,054 + PFR28_00685 0.67 -0.7 729,054 + PFR28_00685 0.67 +0.7 729,054 + PFR28_00685 0.67 -0.3 729,055 - PFR28_00685 0.67 +0.1 729,055 - PFR28_00685 0.67 -0.9 729,055 - PFR28_00685 0.67 +0.6 729,055 - PFR28_00685 0.67 -0.5 729,055 - PFR28_00685 0.67 -0.9 729,305 - +1.1 729,333 + -0.9 729,392 + -0.4 729,393 - +0.2
Or see this region's nucleotide sequence