Experiment: Cytidine (N) 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
     
    
        
         
     
    
        
         
     
    
        
         
     
    
         
     
     
 
500 nt yagM and yagN are separated by 339 nucleotides yagN and intF are separated by 116 nucleotides intF and ptwF are separated by 109 nucleotides ptwF and yagP are separated by 126 nucleotides yagP and ykgT overlap by 6 nucleotides ykgT and yagQ overlap by 22 nucleotides  
        
        b0279: yagM - CP4-6 prophage; predicted protein (NCBI), at 293,169 to 294,023 
         
        yagM 
         
        
        b0280: yagN - CP4-6 prophage; predicted protein (NCBI), at 294,363 to 294,803 
         
        yagN 
         
        
        b0281: intF - CP4-6 prophage; predicted phage integrase (NCBI), at 294,920 to 296,320 
         
        intF 
         
        
        b4629: ptwF - tRNA-OTHER (RefSeq), at 296,430 to 296,478 
         
        ptwF 
         
        
        b4694: yagP - no description, at 296,605 to 296,943 
         
        yagP 
         
        
        b4695: ykgT - no description, at 296,938 to 297,015 
         
        ykgT 
         
        
        b0283: yagQ - hypothetical protein (NCBI), at 296,994 to 297,950 
         
        yagQ 
         Position (kb)  
294 
 
295 
 
296 
 
297 Strain fitness (log2 ratio)  
-2 
 
-1 
 
0 
 
1 
 
2 at 293.928 kb on + strand, within yagM at 293.928 kb on + strand, within yagM at 293.933 kb on + strand, within yagM at 293.936 kb on + strand, within yagM at 293.937 kb on - strand, within yagM at 293.938 kb on + strand at 293.938 kb on + strand at 293.938 kb on + strand at 293.960 kb on - strand at 294.107 kb on - strand at 294.107 kb on - strand at 294.195 kb on - strand at 294.200 kb on - strand at 294.200 kb on - strand at 294.206 kb on - strand at 294.206 kb on - strand at 294.225 kb on - strand at 294.240 kb on - strand at 294.240 kb on - strand at 294.240 kb on - strand at 294.240 kb on - strand at 294.275 kb on - strand at 294.330 kb on - strand at 294.338 kb on - strand at 294.387 kb on - strand at 294.387 kb on - strand at 294.389 kb on - strand at 294.389 kb on - strand at 294.423 kb on + strand, within yagN at 294.453 kb on - strand, within yagN at 294.453 kb on - strand, within yagN at 294.466 kb on + strand, within yagN at 294.470 kb on - strand, within yagN at 294.480 kb on - strand, within yagN at 294.488 kb on - strand, within yagN at 294.488 kb on - strand, within yagN at 294.488 kb on - strand, within yagN at 294.490 kb on + strand, within yagN at 294.491 kb on + strand, within yagN at 294.523 kb on + strand, within yagN at 294.523 kb on + strand, within yagN at 294.523 kb on + strand, within yagN at 294.531 kb on + strand, within yagN at 294.539 kb on - strand, within yagN at 294.663 kb on - strand, within yagN at 294.667 kb on + strand, within yagN at 294.686 kb on - strand, within yagN at 294.698 kb on + strand, within yagN at 294.761 kb on - strand at 294.761 kb on - strand at 294.777 kb on + strand at 294.809 kb on + strand at 294.822 kb on - strand at 294.847 kb on + strand at 294.855 kb on - strand at 295.050 kb on + strand at 295.050 kb on + strand at 295.175 kb on + strand, within intF at 295.183 kb on - strand, within intF at 295.183 kb on - strand, within intF at 295.238 kb on - strand, within intF at 295.399 kb on - strand, within intF at 295.399 kb on - strand, within intF at 295.470 kb on + strand, within intF at 295.470 kb on + strand, within intF at 295.508 kb on + strand, within intF at 295.567 kb on + strand, within intF at 295.579 kb on - strand, within intF at 295.579 kb on - strand, within intF at 295.626 kb on + strand, within intF at 295.648 kb on - strand, within intF at 295.648 kb on - strand, within intF at 295.650 kb on + strand, within intF at 295.654 kb on + strand, within intF at 295.680 kb on - strand, within intF at 295.692 kb on + strand, within intF at 295.698 kb on - strand, within intF at 295.699 kb on + strand, within intF at 295.700 kb on - strand, within intF at 295.700 kb on - strand, within intF at 295.724 kb on - strand, within intF at 295.724 kb on - strand, within intF at 295.796 kb on + strand, within intF at 295.826 kb on - strand, within intF at 295.826 kb on - strand, within intF at 295.974 kb on - strand, within intF at 296.002 kb on + strand, within intF at 296.012 kb on + strand, within intF at 296.012 kb on + strand, within intF at 296.027 kb on + strand, within intF at 296.059 kb on + strand, within intF at 296.059 kb on + strand, within intF at 296.202 kb on - strand at 296.204 kb on - strand at 296.204 kb on - strand at 296.456 kb on - strand, within ptwF at 296.543 kb on + strand at 296.543 kb on + strand at 296.551 kb on - strand at 296.635 kb on - strand at 296.635 kb on - strand at 296.655 kb on + strand, within yagP at 296.813 kb on + strand, within yagP at 296.813 kb on + strand, within yagP at 296.818 kb on - strand, within yagP at 296.818 kb on - strand, within yagP at 296.896 kb on + strand, within yagP at 296.930 kb on + strand at 296.991 kb on + strand, within ykgT at 296.993 kb on - strand, within ykgT at 296.993 kb on - strand, within ykgT at 297.046 kb on - strand at 297.064 kb on + strand at 297.064 kb on + strand at 297.068 kb on + strand at 297.068 kb on + strand at 297.082 kb on - strand at 297.111 kb on - strand, within yagQ at 297.111 kb on - strand, within yagQ at 297.144 kb on - strand, within yagQ at 297.144 kb on - strand, within yagQ at 297.185 kb on + strand, within yagQ at 297.185 kb on + strand, within yagQ at 297.193 kb on - strand, within yagQ at 297.226 kb on - strand, within yagQ at 297.226 kb on - strand, within yagQ at 297.316 kb on - strand, within yagQ at 297.316 kb on - strand, within yagQ  
 
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction   Cytidine (N)             remove   293,928   +  yagM  b0279   0.89  -0.1   293,928   +  yagM  b0279   0.89  -0.4   293,933   +  yagM  b0279   0.89  +0.7   293,936   +  yagM  b0279   0.90  -0.0   293,937   -  yagM  b0279   0.90  -0.2   293,938   +        +2.7   293,938   +        -0.8   293,938   +        +0.7   293,960   -        -0.1   294,107   -        +1.7   294,107   -        -2.2   294,195   -        -0.3   294,200   -        -0.3   294,200   -        -0.9   294,206   -        +0.1   294,206   -        +0.7   294,225   -        +0.5   294,240   -        -0.2   294,240   -        +0.9   294,240   -        +0.1   294,240   -        +0.3   294,275   -        -0.9   294,330   -        +0.8   294,338   -        -0.0   294,387   -        +0.1   294,387   -        -0.3   294,389   -        +0.3   294,389   -        +0.1   294,423   +  yagN  b0280   0.14  -0.6   294,453   -  yagN  b0280   0.20  +0.1   294,453   -  yagN  b0280   0.20  +0.2   294,466   +  yagN  b0280   0.23  -0.6   294,470   -  yagN  b0280   0.24  -0.3   294,480   -  yagN  b0280   0.27  +0.7   294,488   -  yagN  b0280   0.28  +0.3   294,488   -  yagN  b0280   0.28  +0.2   294,488   -  yagN  b0280   0.28  +0.7   294,490   +  yagN  b0280   0.29  -0.3   294,491   +  yagN  b0280   0.29  -1.7   294,523   +  yagN  b0280   0.36  -0.9   294,523   +  yagN  b0280   0.36  +0.4   294,523   +  yagN  b0280   0.36  -0.8   294,531   +  yagN  b0280   0.38  -0.1   294,539   -  yagN  b0280   0.40  +0.1   294,663   -  yagN  b0280   0.68  +1.7   294,667   +  yagN  b0280   0.69  -0.2   294,686   -  yagN  b0280   0.73  +0.3   294,698   +  yagN  b0280   0.76  +0.9   294,761   -        +1.1   294,761   -        -0.3   294,777   +        -0.1   294,809   +        +0.4   294,822   -        -0.2   294,847   +        -0.5   294,855   -        +1.2   295,050   +        -0.5   295,050   +        +0.1   295,175   +  intF  b0281   0.18  -1.3   295,183   -  intF  b0281   0.19  -0.3   295,183   -  intF  b0281   0.19  +1.2   295,238   -  intF  b0281   0.23  +0.2   295,399   -  intF  b0281   0.34  -0.5   295,399   -  intF  b0281   0.34  -0.9   295,470   +  intF  b0281   0.39  -1.3   295,470   +  intF  b0281   0.39  -0.4   295,508   +  intF  b0281   0.42  -1.0   295,567   +  intF  b0281   0.46  -0.5   295,579   -  intF  b0281   0.47  -0.2   295,579   -  intF  b0281   0.47  -0.7   295,626   +  intF  b0281   0.50  +0.8   295,648   -  intF  b0281   0.52  -0.9   295,648   -  intF  b0281   0.52  -1.5   295,650   +  intF  b0281   0.52  +0.3   295,654   +  intF  b0281   0.52  +0.1   295,680   -  intF  b0281   0.54  +0.5   295,692   +  intF  b0281   0.55  -0.2   295,698   -  intF  b0281   0.56  +0.1   295,699   +  intF  b0281   0.56  +0.4   295,700   -  intF  b0281   0.56  -0.2   295,700   -  intF  b0281   0.56  +0.2   295,724   -  intF  b0281   0.57  -0.6   295,724   -  intF  b0281   0.57  +2.2   295,796   +  intF  b0281   0.63  -1.2   295,826   -  intF  b0281   0.65  -0.9   295,826   -  intF  b0281   0.65  +1.7   295,974   -  intF  b0281   0.75  +0.1   296,002   +  intF  b0281   0.77  -0.7   296,012   +  intF  b0281   0.78  -0.2   296,012   +  intF  b0281   0.78  -1.1   296,027   +  intF  b0281   0.79  -0.4   296,059   +  intF  b0281   0.81  +0.1   296,059   +  intF  b0281   0.81  -0.6   296,202   -        -2.3   296,204   -        +1.2   296,204   -        +1.0   296,456   -  ptwF  b4629   0.53  -0.3   296,543   +        -0.5   296,543   +        +1.0   296,551   -        -1.3   296,635   -        -0.8   296,635   -        +0.7   296,655   +  yagP  b4694   0.15  +0.0   296,813   +  yagP  b4694   0.61  +0.7   296,813   +  yagP  b4694   0.61  +1.1   296,818   -  yagP  b4694   0.63  -1.3   296,818   -  yagP  b4694   0.63  +0.3   296,896   +  yagP  b4694   0.86  +1.1   296,930   +        +0.3   296,991   +  ykgT  b4695   0.68  +0.4   296,993   -  ykgT  b4695   0.71  -1.3   296,993   -  ykgT  b4695   0.71  +0.7   297,046   -        +0.0   297,064   +        +0.1   297,064   +        -0.8   297,068   +        +0.1   297,068   +        -1.3   297,082   -        -0.0   297,111   -  yagQ  b0283   0.12  -0.0   297,111   -  yagQ  b0283   0.12  -1.0   297,144   -  yagQ  b0283   0.16  +0.2   297,144   -  yagQ  b0283   0.16  +0.4   297,185   +  yagQ  b0283   0.20  +0.1   297,185   +  yagQ  b0283   0.20  +0.5   297,193   -  yagQ  b0283   0.21  +0.3   297,226   -  yagQ  b0283   0.24  +1.2   297,226   -  yagQ  b0283   0.24  +1.9   297,316   -  yagQ  b0283   0.34  -0.8   297,316   -  yagQ  b0283   0.34  +1.9 
 
Or see this region's nucleotide sequence