Experiment: alpha-Lactose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu1324 and Atu1325 are separated by 208 nucleotides Atu1325 and Atu1326 overlap by 4 nucleotides Atu1326 and Atu1327 are separated by 109 nucleotides Atu1327 and Atu1328 are separated by 131 nucleotides
Atu1324: Atu1324 - glutamyl-tRNA amidotransferase subunit B, at 1,312,454 to 1,313,959
Atu1324
Atu1325: Atu1325 - acetyltransferase, at 1,314,168 to 1,314,665
Atu1325
Atu1326: Atu1326 - acetyltransferase, at 1,314,662 to 1,315,135
Atu1326
Atu1327: Atu1327 - NADH-ubiquinone oxidoreductase, at 1,315,245 to 1,315,643
Atu1327
Atu1328: Atu1328 - hypothetical protein, at 1,315,775 to 1,316,212
Atu1328
Position (kb)
1314
1315
1316 Strain fitness (log2 ratio)
-1
0
1 at 1314.050 kb on + strand at 1314.079 kb on + strand at 1314.442 kb on - strand, within Atu1325 at 1314.492 kb on + strand, within Atu1325 at 1314.581 kb on - strand, within Atu1325 at 1314.813 kb on - strand, within Atu1326 at 1314.917 kb on - strand, within Atu1326 at 1314.939 kb on - strand, within Atu1326 at 1314.939 kb on - strand, within Atu1326 at 1315.017 kb on + strand, within Atu1326 at 1315.039 kb on + strand, within Atu1326 at 1315.496 kb on + strand, within Atu1327 at 1315.498 kb on + strand, within Atu1327 at 1315.663 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction alpha-Lactose (C) remove 1,314,050 + +0.0 1,314,079 + +0.0 1,314,442 - Atu1325 0.55 +0.3 1,314,492 + Atu1325 0.65 +0.0 1,314,581 - Atu1325 0.83 +0.8 1,314,813 - Atu1326 0.32 +0.9 1,314,917 - Atu1326 0.54 +0.2 1,314,939 - Atu1326 0.58 +0.1 1,314,939 - Atu1326 0.58 +0.2 1,315,017 + Atu1326 0.75 +0.4 1,315,039 + Atu1326 0.80 +1.3 1,315,496 + Atu1327 0.63 -0.2 1,315,498 + Atu1327 0.63 -1.1 1,315,663 - +0.0
Or see this region's nucleotide sequence