Experiment: m.b. Spectinomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Echvi_0127 and Echvi_0128 are separated by 73 nucleotides Echvi_0128 and Echvi_0129 are separated by 20 nucleotides Echvi_0129 and Echvi_0130 are separated by 26 nucleotides Echvi_0130 and Echvi_0131 are separated by 121 nucleotides
Echvi_0127: Echvi_0127 - hypothetical protein, at 127,202 to 127,438
_0127
Echvi_0128: Echvi_0128 - hypothetical protein, at 127,512 to 127,607
_0128
Echvi_0129: Echvi_0129 - hypothetical protein, at 127,628 to 128,110
_0129
Echvi_0130: Echvi_0130 - Predicted metal-dependent hydrolase with the TIM-barrel fold, at 128,137 to 129,777
_0130
Echvi_0131: Echvi_0131 - DNA uptake protein and related DNA-binding proteins, at 129,899 to 130,612
_0131
Position (kb) 128
129
130 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 127.185 kb on + strand at 127.193 kb on + strand at 127.198 kb on + strand at 127.198 kb on + strand at 127.200 kb on + strand at 127.240 kb on + strand, within Echvi_0127 at 127.250 kb on + strand, within Echvi_0127 at 127.280 kb on - strand, within Echvi_0127 at 127.424 kb on - strand at 127.424 kb on - strand at 127.536 kb on - strand, within Echvi_0128 at 127.650 kb on - strand at 127.659 kb on + strand at 127.659 kb on + strand at 127.660 kb on - strand at 127.660 kb on - strand at 127.660 kb on - strand at 127.844 kb on + strand, within Echvi_0129 at 127.844 kb on + strand, within Echvi_0129 at 127.847 kb on - strand, within Echvi_0129 at 127.847 kb on - strand, within Echvi_0129 at 127.873 kb on - strand, within Echvi_0129 at 127.942 kb on + strand, within Echvi_0129 at 127.942 kb on + strand, within Echvi_0129 at 127.943 kb on - strand, within Echvi_0129 at 127.943 kb on - strand, within Echvi_0129 at 127.943 kb on - strand, within Echvi_0129 at 127.943 kb on - strand, within Echvi_0129 at 127.992 kb on - strand, within Echvi_0129 at 128.015 kb on - strand, within Echvi_0129 at 128.026 kb on + strand, within Echvi_0129 at 128.045 kb on - strand, within Echvi_0129 at 128.105 kb on - strand at 128.114 kb on + strand at 128.114 kb on + strand at 128.114 kb on + strand at 128.114 kb on + strand at 128.115 kb on - strand at 128.115 kb on - strand at 128.115 kb on - strand at 128.120 kb on - strand at 128.127 kb on - strand at 128.178 kb on + strand at 128.179 kb on - strand at 128.217 kb on - strand at 128.217 kb on - strand at 128.245 kb on + strand at 128.245 kb on + strand at 128.245 kb on + strand at 128.245 kb on + strand at 128.246 kb on - strand at 128.246 kb on - strand at 128.246 kb on - strand at 128.246 kb on - strand at 128.246 kb on - strand at 128.246 kb on - strand at 128.246 kb on - strand at 128.247 kb on + strand at 128.304 kb on + strand, within Echvi_0130 at 128.748 kb on - strand, within Echvi_0130 at 128.859 kb on + strand, within Echvi_0130 at 128.862 kb on + strand, within Echvi_0130 at 128.948 kb on - strand, within Echvi_0130 at 128.957 kb on + strand, within Echvi_0130 at 128.957 kb on + strand, within Echvi_0130 at 128.957 kb on + strand, within Echvi_0130 at 128.957 kb on + strand, within Echvi_0130 at 128.983 kb on + strand, within Echvi_0130 at 128.983 kb on + strand, within Echvi_0130 at 128.984 kb on - strand, within Echvi_0130 at 128.986 kb on + strand, within Echvi_0130 at 128.986 kb on + strand, within Echvi_0130 at 128.986 kb on + strand, within Echvi_0130 at 128.986 kb on + strand, within Echvi_0130 at 128.987 kb on - strand, within Echvi_0130 at 129.081 kb on - strand, within Echvi_0130 at 129.236 kb on - strand, within Echvi_0130 at 129.236 kb on - strand, within Echvi_0130 at 129.328 kb on + strand, within Echvi_0130 at 129.329 kb on - strand, within Echvi_0130 at 129.373 kb on - strand, within Echvi_0130 at 129.373 kb on - strand, within Echvi_0130 at 129.373 kb on - strand, within Echvi_0130 at 129.376 kb on + strand, within Echvi_0130 at 129.376 kb on + strand, within Echvi_0130 at 129.376 kb on + strand, within Echvi_0130 at 129.377 kb on - strand, within Echvi_0130 at 129.377 kb on - strand, within Echvi_0130 at 129.377 kb on - strand, within Echvi_0130 at 129.377 kb on - strand, within Echvi_0130 at 129.421 kb on + strand, within Echvi_0130 at 129.423 kb on + strand at 129.424 kb on - strand, within Echvi_0130 at 129.424 kb on - strand, within Echvi_0130 at 129.424 kb on - strand, within Echvi_0130 at 129.623 kb on - strand at 129.654 kb on + strand at 129.655 kb on - strand at 129.740 kb on + strand at 129.741 kb on - strand at 129.741 kb on - strand at 129.751 kb on + strand at 129.751 kb on + strand at 129.751 kb on + strand at 129.751 kb on + strand at 129.751 kb on + strand at 129.751 kb on + strand at 129.752 kb on - strand at 129.844 kb on + strand at 129.887 kb on + strand at 129.933 kb on + strand at 129.934 kb on - strand at 130.050 kb on + strand, within Echvi_0131 at 130.051 kb on - strand, within Echvi_0131 at 130.059 kb on + strand, within Echvi_0131 at 130.083 kb on - strand, within Echvi_0131 at 130.083 kb on - strand, within Echvi_0131 at 130.094 kb on - strand, within Echvi_0131 at 130.098 kb on - strand, within Echvi_0131 at 130.107 kb on + strand, within Echvi_0131 at 130.108 kb on - strand, within Echvi_0131 at 130.133 kb on - strand, within Echvi_0131 at 130.133 kb on - strand, within Echvi_0131 at 130.145 kb on + strand, within Echvi_0131 at 130.199 kb on - strand, within Echvi_0131 at 130.331 kb on - strand, within Echvi_0131 at 130.339 kb on - strand, within Echvi_0131 at 130.429 kb on + strand, within Echvi_0131 at 130.429 kb on + strand, within Echvi_0131 at 130.430 kb on - strand, within Echvi_0131 at 130.449 kb on + strand, within Echvi_0131 at 130.449 kb on + strand, within Echvi_0131 at 130.486 kb on - strand, within Echvi_0131 at 130.524 kb on - strand, within Echvi_0131 at 130.524 kb on - strand, within Echvi_0131 at 130.579 kb on - strand at 130.599 kb on - strand at 130.615 kb on + strand at 130.616 kb on - strand at 130.639 kb on - strand at 130.775 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.06 mg/ml remove 127,185 + +1.9 127,193 + +0.8 127,198 + +0.2 127,198 + -0.3 127,200 + +0.1 127,240 + Echvi_0127 0.16 -1.2 127,250 + Echvi_0127 0.20 -1.7 127,280 - Echvi_0127 0.33 +0.3 127,424 - +0.3 127,424 - +0.4 127,536 - Echvi_0128 0.25 +0.2 127,650 - -0.4 127,659 + -1.0 127,659 + +0.3 127,660 - +0.4 127,660 - +0.3 127,660 - +0.3 127,844 + Echvi_0129 0.45 -0.6 127,844 + Echvi_0129 0.45 -1.1 127,847 - Echvi_0129 0.45 +0.1 127,847 - Echvi_0129 0.45 +0.3 127,873 - Echvi_0129 0.51 +0.8 127,942 + Echvi_0129 0.65 +0.3 127,942 + Echvi_0129 0.65 -0.6 127,943 - Echvi_0129 0.65 +0.9 127,943 - Echvi_0129 0.65 +0.2 127,943 - Echvi_0129 0.65 -2.1 127,943 - Echvi_0129 0.65 +0.2 127,992 - Echvi_0129 0.75 +1.1 128,015 - Echvi_0129 0.80 +0.9 128,026 + Echvi_0129 0.82 +0.8 128,045 - Echvi_0129 0.86 +0.4 128,105 - +1.9 128,114 + -1.0 128,114 + +0.1 128,114 + -1.1 128,114 + -1.2 128,115 - -0.0 128,115 - +0.6 128,115 - +0.3 128,120 - +0.9 128,127 - +0.9 128,178 + +1.1 128,179 - -0.5 128,217 - +1.8 128,217 - -0.5 128,245 + -1.8 128,245 + +0.4 128,245 + +0.3 128,245 + +0.3 128,246 - +0.3 128,246 - -0.1 128,246 - -0.4 128,246 - -0.2 128,246 - -0.1 128,246 - -0.6 128,246 - +0.1 128,247 + +0.8 128,304 + Echvi_0130 0.10 -0.7 128,748 - Echvi_0130 0.37 -2.8 128,859 + Echvi_0130 0.44 +0.9 128,862 + Echvi_0130 0.44 +0.1 128,948 - Echvi_0130 0.49 -0.1 128,957 + Echvi_0130 0.50 +1.4 128,957 + Echvi_0130 0.50 +0.1 128,957 + Echvi_0130 0.50 +0.5 128,957 + Echvi_0130 0.50 +0.6 128,983 + Echvi_0130 0.52 -0.6 128,983 + Echvi_0130 0.52 +1.2 128,984 - Echvi_0130 0.52 -1.8 128,986 + Echvi_0130 0.52 -0.1 128,986 + Echvi_0130 0.52 +1.0 128,986 + Echvi_0130 0.52 -1.6 128,986 + Echvi_0130 0.52 -1.0 128,987 - Echvi_0130 0.52 +1.2 129,081 - Echvi_0130 0.58 +1.7 129,236 - Echvi_0130 0.67 +0.9 129,236 - Echvi_0130 0.67 +1.1 129,328 + Echvi_0130 0.73 -1.1 129,329 - Echvi_0130 0.73 +0.3 129,373 - Echvi_0130 0.75 -0.2 129,373 - Echvi_0130 0.75 -0.5 129,373 - Echvi_0130 0.75 +0.7 129,376 + Echvi_0130 0.76 +1.1 129,376 + Echvi_0130 0.76 +0.9 129,376 + Echvi_0130 0.76 +0.5 129,377 - Echvi_0130 0.76 -2.1 129,377 - Echvi_0130 0.76 -0.5 129,377 - Echvi_0130 0.76 +1.0 129,377 - Echvi_0130 0.76 -1.5 129,421 + Echvi_0130 0.78 +1.4 129,423 + +0.9 129,424 - Echvi_0130 0.78 -0.3 129,424 - Echvi_0130 0.78 +0.0 129,424 - Echvi_0130 0.78 +1.1 129,623 - +0.7 129,654 + -0.9 129,655 - -1.3 129,740 + +0.4 129,741 - -0.6 129,741 - -1.2 129,751 + -0.1 129,751 + +1.5 129,751 + -1.7 129,751 + +0.4 129,751 + +2.3 129,751 + +1.2 129,752 - +0.0 129,844 + -0.5 129,887 + +0.3 129,933 + +0.5 129,934 - -0.3 130,050 + Echvi_0131 0.21 -0.9 130,051 - Echvi_0131 0.21 -0.1 130,059 + Echvi_0131 0.22 +0.2 130,083 - Echvi_0131 0.26 -1.1 130,083 - Echvi_0131 0.26 +0.9 130,094 - Echvi_0131 0.27 -0.9 130,098 - Echvi_0131 0.28 +0.3 130,107 + Echvi_0131 0.29 -0.2 130,108 - Echvi_0131 0.29 -0.5 130,133 - Echvi_0131 0.33 -0.3 130,133 - Echvi_0131 0.33 -0.9 130,145 + Echvi_0131 0.34 +0.1 130,199 - Echvi_0131 0.42 -0.3 130,331 - Echvi_0131 0.61 -0.1 130,339 - Echvi_0131 0.62 +1.2 130,429 + Echvi_0131 0.74 -0.7 130,429 + Echvi_0131 0.74 -2.6 130,430 - Echvi_0131 0.74 +0.1 130,449 + Echvi_0131 0.77 -0.5 130,449 + Echvi_0131 0.77 +0.7 130,486 - Echvi_0131 0.82 +0.9 130,524 - Echvi_0131 0.88 -0.1 130,524 - Echvi_0131 0.88 -0.1 130,579 - -0.1 130,599 - -2.1 130,615 + -2.1 130,616 - +0.1 130,639 - -1.5 130,775 - +1.2
Or see this region's nucleotide sequence