Experiment: m.b. Spectinomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Echvi_0088 and Echvi_0089 are separated by 67 nucleotides Echvi_0089 and Echvi_0090 are separated by 191 nucleotides Echvi_0090 and Echvi_0091 are separated by 324 nucleotides
Echvi_0088: Echvi_0088 - thiamine-monophosphate kinase, at 87,885 to 88,934
_0088
Echvi_0089: Echvi_0089 - methylmalonyl-CoA epimerase, at 89,002 to 89,406
_0089
Echvi_0090: Echvi_0090 - Iron-sulfur cluster assembly accessory protein, at 89,598 to 89,924
_0090
Echvi_0091: Echvi_0091 - glycine cleavage system T protein, at 90,249 to 91,343
_0091
Position (kb) 89
90 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 88.935 kb on - strand at 88.943 kb on - strand at 89.007 kb on + strand at 89.007 kb on + strand at 89.007 kb on + strand at 89.007 kb on + strand at 89.007 kb on + strand at 89.007 kb on + strand at 89.007 kb on + strand at 89.008 kb on - strand at 89.066 kb on - strand, within Echvi_0089 at 89.071 kb on + strand, within Echvi_0089 at 89.071 kb on + strand, within Echvi_0089 at 89.072 kb on - strand, within Echvi_0089 at 89.072 kb on - strand, within Echvi_0089 at 89.154 kb on + strand, within Echvi_0089 at 89.155 kb on - strand, within Echvi_0089 at 89.155 kb on - strand, within Echvi_0089 at 89.155 kb on - strand, within Echvi_0089 at 89.445 kb on + strand at 89.450 kb on + strand at 89.450 kb on + strand at 89.451 kb on - strand at 89.454 kb on + strand at 89.508 kb on + strand at 89.575 kb on + strand at 89.575 kb on + strand at 89.578 kb on + strand at 89.591 kb on - strand at 89.597 kb on + strand at 89.597 kb on + strand at 89.684 kb on - strand, within Echvi_0090 at 89.761 kb on + strand, within Echvi_0090 at 89.762 kb on - strand, within Echvi_0090 at 89.811 kb on + strand, within Echvi_0090 at 89.944 kb on - strand at 89.944 kb on - strand at 89.946 kb on - strand at 89.949 kb on - strand at 89.951 kb on + strand at 89.952 kb on - strand at 89.975 kb on - strand at 90.084 kb on + strand at 90.085 kb on - strand at 90.085 kb on - strand at 90.111 kb on + strand at 90.111 kb on + strand at 90.111 kb on + strand at 90.111 kb on + strand at 90.112 kb on - strand at 90.112 kb on - strand at 90.134 kb on - strand at 90.134 kb on - strand at 90.134 kb on - strand at 90.216 kb on - strand at 90.246 kb on + strand at 90.246 kb on + strand at 90.368 kb on + strand, within Echvi_0091 at 90.369 kb on - strand, within Echvi_0091 at 90.423 kb on - strand, within Echvi_0091 at 90.580 kb on - strand, within Echvi_0091 at 90.825 kb on - strand, within Echvi_0091 at 90.847 kb on + strand, within Echvi_0091 at 90.847 kb on + strand, within Echvi_0091 at 90.848 kb on - strand, within Echvi_0091 at 90.877 kb on + strand, within Echvi_0091
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.06 mg/ml remove 88,935 - +0.8 88,943 - -1.0 89,007 + +0.7 89,007 + +0.5 89,007 + -1.3 89,007 + -1.1 89,007 + -0.1 89,007 + -0.7 89,007 + -0.3 89,008 - -0.1 89,066 - Echvi_0089 0.16 -0.9 89,071 + Echvi_0089 0.17 -1.3 89,071 + Echvi_0089 0.17 +1.6 89,072 - Echvi_0089 0.17 +0.6 89,072 - Echvi_0089 0.17 +0.4 89,154 + Echvi_0089 0.38 +3.4 89,155 - Echvi_0089 0.38 -0.9 89,155 - Echvi_0089 0.38 +0.5 89,155 - Echvi_0089 0.38 +0.3 89,445 + -0.9 89,450 + +0.2 89,450 + +0.4 89,451 - +1.3 89,454 + -0.8 89,508 + -0.5 89,575 + -0.3 89,575 + -1.0 89,578 + -0.1 89,591 - -0.7 89,597 + -1.1 89,597 + -1.3 89,684 - Echvi_0090 0.26 +0.9 89,761 + Echvi_0090 0.50 -0.2 89,762 - Echvi_0090 0.50 -1.6 89,811 + Echvi_0090 0.65 -0.7 89,944 - +0.9 89,944 - +0.2 89,946 - +0.6 89,949 - -0.1 89,951 + -1.1 89,952 - -0.8 89,975 - -0.6 90,084 + +0.5 90,085 - -0.4 90,085 - -1.5 90,111 + +0.4 90,111 + +0.7 90,111 + -1.4 90,111 + -0.8 90,112 - +0.5 90,112 - -0.1 90,134 - +0.0 90,134 - +0.4 90,134 - +0.9 90,216 - -0.5 90,246 + -0.9 90,246 + -0.6 90,368 + Echvi_0091 0.11 -1.6 90,369 - Echvi_0091 0.11 -0.7 90,423 - Echvi_0091 0.16 -0.9 90,580 - Echvi_0091 0.30 -0.7 90,825 - Echvi_0091 0.53 -1.3 90,847 + Echvi_0091 0.55 -2.0 90,847 + Echvi_0091 0.55 -2.4 90,848 - Echvi_0091 0.55 +0.5 90,877 + Echvi_0091 0.57 -0.5
Or see this region's nucleotide sequence