Experiment: L-ascorbate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu2612 and Atu2613 are separated by 35 nucleotides Atu2613 and Atu2614 are separated by 544 nucleotides Atu2614 and Atu2615 are separated by 175 nucleotides
Atu2612: Atu2612 - 1-deoxy-D-xylulose 5-phosphate reductoisomerase, at 2,590,929 to 2,592,122
Atu2612
Atu2613: Atu2613 - 5-aminolevulinate synthase, at 2,592,158 to 2,593,201
Atu2613
Atu2614: Atu2614 - hypothetical protein, at 2,593,746 to 2,593,997
Atu2614
Atu2615: Atu2615 - hypothetical protein, at 2,594,173 to 2,596,098
Atu2615
Position (kb)
2592
2593
2594 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2592.107 kb on + strand at 2592.128 kb on + strand at 2592.136 kb on - strand at 2592.136 kb on - strand at 2592.136 kb on - strand at 2592.164 kb on - strand at 2592.164 kb on - strand at 2592.164 kb on - strand at 2593.382 kb on + strand at 2593.390 kb on - strand at 2593.571 kb on + strand at 2593.574 kb on + strand at 2593.724 kb on + strand at 2593.731 kb on + strand at 2593.772 kb on + strand, within Atu2614 at 2593.792 kb on + strand, within Atu2614 at 2593.842 kb on + strand, within Atu2614 at 2593.842 kb on + strand, within Atu2614 at 2593.842 kb on + strand, within Atu2614 at 2593.850 kb on - strand, within Atu2614 at 2593.850 kb on - strand, within Atu2614 at 2593.914 kb on + strand, within Atu2614 at 2593.922 kb on - strand, within Atu2614 at 2593.938 kb on - strand, within Atu2614 at 2593.945 kb on + strand, within Atu2614 at 2593.971 kb on - strand, within Atu2614 at 2593.973 kb on + strand at 2593.973 kb on + strand at 2593.973 kb on + strand at 2593.981 kb on - strand at 2594.008 kb on + strand at 2594.009 kb on - strand at 2594.016 kb on - strand at 2594.182 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-ascorbate (C) remove 2,592,107 + +0.6 2,592,128 + +0.5 2,592,136 - +0.6 2,592,136 - +0.1 2,592,136 - +0.1 2,592,164 - -1.2 2,592,164 - -0.4 2,592,164 - +0.2 2,593,382 + -0.1 2,593,390 - +1.1 2,593,571 + -0.4 2,593,574 + -0.5 2,593,724 + -2.6 2,593,731 + -2.4 2,593,772 + Atu2614 0.10 -3.7 2,593,792 + Atu2614 0.18 -0.2 2,593,842 + Atu2614 0.38 -2.8 2,593,842 + Atu2614 0.38 -4.1 2,593,842 + Atu2614 0.38 -3.6 2,593,850 - Atu2614 0.41 +1.2 2,593,850 - Atu2614 0.41 -3.6 2,593,914 + Atu2614 0.67 -4.4 2,593,922 - Atu2614 0.70 -1.2 2,593,938 - Atu2614 0.76 -2.2 2,593,945 + Atu2614 0.79 -3.3 2,593,971 - Atu2614 0.89 -2.5 2,593,973 + -2.7 2,593,973 + -3.9 2,593,973 + -2.6 2,593,981 - -2.5 2,594,008 + -1.8 2,594,009 - -0.2 2,594,016 - -1.4 2,594,182 + -1.3
Or see this region's nucleotide sequence