Experiment: L-ascorbate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu1351 and Atu1352 are separated by 179 nucleotides Atu1352 and Atu1353 are separated by 79 nucleotides Atu1353 and Atu1354 are separated by 19 nucleotides Atu1354 and Atu1355 are separated by 14 nucleotides
Atu1351: Atu1351 - hypothetical protein, at 1,345,934 to 1,346,560
Atu1351
Atu1352: Atu1352 - dihydropteroate synthase, at 1,346,740 to 1,347,525
Atu1352
Atu1353: Atu1353 - dihydroneopterin aldolase, at 1,347,605 to 1,347,970
Atu1353
Atu1354: Atu1354 - 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase, at 1,347,990 to 1,348,490
Atu1354
Atu1355: Atu1355 - hypothetical protein, at 1,348,505 to 1,349,065
Atu1355
Position (kb)
1346
1347
1348 Strain fitness (log2 ratio)
-2
-1
0
1 at 1345.848 kb on + strand at 1345.957 kb on - strand at 1345.962 kb on - strand at 1346.049 kb on + strand, within Atu1351 at 1346.097 kb on - strand, within Atu1351 at 1346.290 kb on - strand, within Atu1351 at 1346.330 kb on - strand, within Atu1351 at 1346.369 kb on + strand, within Atu1351 at 1346.561 kb on + strand at 1346.626 kb on + strand at 1347.971 kb on + strand at 1348.426 kb on + strand, within Atu1354 at 1348.426 kb on + strand, within Atu1354 at 1348.465 kb on - strand at 1348.475 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-ascorbate (C) remove 1,345,848 + +0.5 1,345,957 - -0.4 1,345,962 - +0.0 1,346,049 + Atu1351 0.18 -0.0 1,346,097 - Atu1351 0.26 +0.4 1,346,290 - Atu1351 0.57 +0.0 1,346,330 - Atu1351 0.63 -1.3 1,346,369 + Atu1351 0.69 -0.2 1,346,561 + -0.1 1,346,626 + +0.3 1,347,971 + -0.3 1,348,426 + Atu1354 0.87 +1.4 1,348,426 + Atu1354 0.87 -2.3 1,348,465 - +0.1 1,348,475 + -0.2
Or see this region's nucleotide sequence