Experiment: m.b. D-Cycloserine 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_2233 and B158DRAFT_2234 are separated by 211 nucleotides B158DRAFT_2234 and B158DRAFT_2235 are separated by 18 nucleotides B158DRAFT_2235 and B158DRAFT_2236 are separated by 57 nucleotides
B158DRAFT_2233: B158DRAFT_2233 - Acyl-CoA dehydrogenases, at 385,755 to 388,226
_2233
B158DRAFT_2234: B158DRAFT_2234 - 2-isopropylmalate synthase (EC 2.3.3.13), at 388,438 to 389,592
_2234
B158DRAFT_2235: B158DRAFT_2235 - hypothetical protein, at 389,611 to 390,108
_2235
B158DRAFT_2236: B158DRAFT_2236 - [SSU ribosomal protein S18P]-alanine acetyltransferase (EC 2.3.1.128), at 390,166 to 390,624
_2236
Position (kb) 388
389
390 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 387.526 kb on + strand, within B158DRAFT_2233 at 387.526 kb on + strand, within B158DRAFT_2233 at 387.534 kb on - strand, within B158DRAFT_2233 at 387.534 kb on - strand, within B158DRAFT_2233 at 387.544 kb on + strand, within B158DRAFT_2233 at 387.552 kb on - strand, within B158DRAFT_2233 at 387.564 kb on + strand, within B158DRAFT_2233 at 387.618 kb on + strand, within B158DRAFT_2233 at 387.626 kb on - strand, within B158DRAFT_2233 at 387.638 kb on + strand, within B158DRAFT_2233 at 387.666 kb on + strand, within B158DRAFT_2233 at 387.685 kb on - strand, within B158DRAFT_2233 at 387.692 kb on + strand, within B158DRAFT_2233 at 387.695 kb on - strand, within B158DRAFT_2233 at 387.695 kb on - strand, within B158DRAFT_2233 at 387.700 kb on - strand, within B158DRAFT_2233 at 387.716 kb on - strand, within B158DRAFT_2233 at 387.788 kb on + strand, within B158DRAFT_2233 at 387.789 kb on + strand, within B158DRAFT_2233 at 387.791 kb on - strand, within B158DRAFT_2233 at 387.826 kb on + strand, within B158DRAFT_2233 at 387.826 kb on + strand, within B158DRAFT_2233 at 387.880 kb on + strand, within B158DRAFT_2233 at 387.920 kb on + strand, within B158DRAFT_2233 at 387.920 kb on + strand, within B158DRAFT_2233 at 387.920 kb on + strand, within B158DRAFT_2233 at 387.952 kb on - strand, within B158DRAFT_2233 at 388.011 kb on + strand at 388.011 kb on + strand at 388.011 kb on + strand at 388.031 kb on - strand at 388.039 kb on - strand at 388.057 kb on + strand at 388.065 kb on - strand at 388.065 kb on - strand at 388.070 kb on - strand at 388.080 kb on + strand at 388.090 kb on + strand at 388.151 kb on + strand at 388.319 kb on + strand at 388.327 kb on - strand at 388.339 kb on - strand at 388.344 kb on + strand at 388.474 kb on + strand at 388.551 kb on - strand at 388.559 kb on + strand, within B158DRAFT_2234 at 388.567 kb on - strand, within B158DRAFT_2234 at 388.579 kb on + strand, within B158DRAFT_2234 at 388.642 kb on - strand, within B158DRAFT_2234 at 388.704 kb on + strand, within B158DRAFT_2234 at 388.704 kb on + strand, within B158DRAFT_2234 at 388.704 kb on + strand, within B158DRAFT_2234 at 388.712 kb on - strand, within B158DRAFT_2234 at 388.729 kb on + strand, within B158DRAFT_2234 at 388.737 kb on - strand, within B158DRAFT_2234 at 388.737 kb on - strand, within B158DRAFT_2234 at 388.779 kb on + strand, within B158DRAFT_2234 at 388.792 kb on - strand, within B158DRAFT_2234 at 388.947 kb on + strand, within B158DRAFT_2234 at 388.955 kb on - strand, within B158DRAFT_2234 at 388.961 kb on + strand, within B158DRAFT_2234 at 388.969 kb on - strand, within B158DRAFT_2234 at 388.969 kb on - strand, within B158DRAFT_2234 at 388.969 kb on - strand, within B158DRAFT_2234 at 389.082 kb on + strand, within B158DRAFT_2234 at 389.095 kb on + strand, within B158DRAFT_2234 at 389.103 kb on - strand, within B158DRAFT_2234 at 389.183 kb on - strand, within B158DRAFT_2234 at 389.297 kb on - strand, within B158DRAFT_2234 at 389.373 kb on - strand, within B158DRAFT_2234 at 389.390 kb on + strand, within B158DRAFT_2234 at 389.444 kb on - strand, within B158DRAFT_2234 at 389.484 kb on - strand at 389.576 kb on + strand at 389.586 kb on + strand at 389.586 kb on + strand at 389.678 kb on + strand, within B158DRAFT_2235 at 389.753 kb on - strand, within B158DRAFT_2235 at 389.805 kb on - strand, within B158DRAFT_2235 at 389.814 kb on + strand, within B158DRAFT_2235 at 389.838 kb on - strand, within B158DRAFT_2235 at 389.871 kb on + strand, within B158DRAFT_2235 at 389.871 kb on + strand, within B158DRAFT_2235 at 389.871 kb on + strand, within B158DRAFT_2235 at 389.871 kb on + strand, within B158DRAFT_2235 at 389.871 kb on + strand, within B158DRAFT_2235 at 389.871 kb on + strand, within B158DRAFT_2235 at 389.871 kb on + strand, within B158DRAFT_2235 at 389.871 kb on + strand, within B158DRAFT_2235 at 389.871 kb on + strand, within B158DRAFT_2235 at 389.871 kb on + strand, within B158DRAFT_2235 at 389.871 kb on + strand, within B158DRAFT_2235 at 389.871 kb on + strand, within B158DRAFT_2235 at 389.871 kb on + strand, within B158DRAFT_2235 at 389.871 kb on + strand, within B158DRAFT_2235 at 389.871 kb on + strand, within B158DRAFT_2235 at 389.879 kb on - strand, within B158DRAFT_2235 at 389.879 kb on - strand, within B158DRAFT_2235 at 389.879 kb on - strand, within B158DRAFT_2235 at 389.879 kb on - strand, within B158DRAFT_2235 at 389.879 kb on - strand, within B158DRAFT_2235 at 389.909 kb on + strand, within B158DRAFT_2235 at 389.959 kb on + strand, within B158DRAFT_2235 at 389.982 kb on + strand, within B158DRAFT_2235 at 389.988 kb on + strand, within B158DRAFT_2235 at 389.988 kb on + strand, within B158DRAFT_2235 at 389.998 kb on + strand, within B158DRAFT_2235 at 390.021 kb on + strand, within B158DRAFT_2235 at 390.026 kb on + strand, within B158DRAFT_2235 at 390.034 kb on - strand, within B158DRAFT_2235 at 390.098 kb on + strand at 390.098 kb on + strand at 390.106 kb on - strand at 390.225 kb on + strand, within B158DRAFT_2236 at 390.225 kb on + strand, within B158DRAFT_2236 at 390.233 kb on - strand, within B158DRAFT_2236 at 390.233 kb on - strand, within B158DRAFT_2236 at 390.233 kb on - strand, within B158DRAFT_2236 at 390.235 kb on + strand, within B158DRAFT_2236 at 390.235 kb on + strand, within B158DRAFT_2236 at 390.243 kb on - strand, within B158DRAFT_2236 at 390.243 kb on - strand, within B158DRAFT_2236 at 390.256 kb on - strand, within B158DRAFT_2236 at 390.266 kb on - strand, within B158DRAFT_2236 at 390.268 kb on + strand, within B158DRAFT_2236 at 390.285 kb on + strand, within B158DRAFT_2236 at 390.287 kb on + strand, within B158DRAFT_2236 at 390.287 kb on + strand, within B158DRAFT_2236 at 390.287 kb on + strand, within B158DRAFT_2236 at 390.298 kb on + strand, within B158DRAFT_2236 at 390.300 kb on - strand, within B158DRAFT_2236 at 390.302 kb on + strand, within B158DRAFT_2236 at 390.306 kb on - strand, within B158DRAFT_2236 at 390.319 kb on + strand, within B158DRAFT_2236 at 390.407 kb on + strand, within B158DRAFT_2236 at 390.447 kb on + strand, within B158DRAFT_2236 at 390.455 kb on + strand, within B158DRAFT_2236 at 390.455 kb on + strand, within B158DRAFT_2236 at 390.463 kb on - strand, within B158DRAFT_2236 at 390.493 kb on - strand, within B158DRAFT_2236
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. D-Cycloserine 0.03125 mg/ml remove 387,526 + B158DRAFT_2233 0.72 -0.9 387,526 + B158DRAFT_2233 0.72 -0.8 387,534 - B158DRAFT_2233 0.72 +0.1 387,534 - B158DRAFT_2233 0.72 -0.6 387,544 + B158DRAFT_2233 0.72 -0.4 387,552 - B158DRAFT_2233 0.73 -0.7 387,564 + B158DRAFT_2233 0.73 +0.1 387,618 + B158DRAFT_2233 0.75 -0.7 387,626 - B158DRAFT_2233 0.76 -0.1 387,638 + B158DRAFT_2233 0.76 -0.0 387,666 + B158DRAFT_2233 0.77 +0.5 387,685 - B158DRAFT_2233 0.78 -0.5 387,692 + B158DRAFT_2233 0.78 -0.5 387,695 - B158DRAFT_2233 0.78 -0.5 387,695 - B158DRAFT_2233 0.78 +0.4 387,700 - B158DRAFT_2233 0.79 -1.2 387,716 - B158DRAFT_2233 0.79 -0.4 387,788 + B158DRAFT_2233 0.82 -3.4 387,789 + B158DRAFT_2233 0.82 -0.4 387,791 - B158DRAFT_2233 0.82 +0.4 387,826 + B158DRAFT_2233 0.84 -0.5 387,826 + B158DRAFT_2233 0.84 -0.1 387,880 + B158DRAFT_2233 0.86 +0.2 387,920 + B158DRAFT_2233 0.88 +0.0 387,920 + B158DRAFT_2233 0.88 -1.7 387,920 + B158DRAFT_2233 0.88 +0.9 387,952 - B158DRAFT_2233 0.89 -0.7 388,011 + +0.1 388,011 + +0.0 388,011 + +0.1 388,031 - -0.3 388,039 - -0.2 388,057 + -0.3 388,065 - -0.2 388,065 - +0.2 388,070 - -0.1 388,080 + -1.1 388,090 + -0.1 388,151 + -0.3 388,319 + +0.2 388,327 - -0.2 388,339 - -0.2 388,344 + +0.1 388,474 + -0.1 388,551 - +0.0 388,559 + B158DRAFT_2234 0.10 -0.4 388,567 - B158DRAFT_2234 0.11 -0.1 388,579 + B158DRAFT_2234 0.12 -0.0 388,642 - B158DRAFT_2234 0.18 +0.1 388,704 + B158DRAFT_2234 0.23 +0.3 388,704 + B158DRAFT_2234 0.23 -0.3 388,704 + B158DRAFT_2234 0.23 -0.5 388,712 - B158DRAFT_2234 0.24 -0.1 388,729 + B158DRAFT_2234 0.25 +0.3 388,737 - B158DRAFT_2234 0.26 -1.0 388,737 - B158DRAFT_2234 0.26 -0.7 388,779 + B158DRAFT_2234 0.30 -0.1 388,792 - B158DRAFT_2234 0.31 -0.8 388,947 + B158DRAFT_2234 0.44 -0.2 388,955 - B158DRAFT_2234 0.45 -0.4 388,961 + B158DRAFT_2234 0.45 -0.7 388,969 - B158DRAFT_2234 0.46 -0.4 388,969 - B158DRAFT_2234 0.46 -2.2 388,969 - B158DRAFT_2234 0.46 +0.1 389,082 + B158DRAFT_2234 0.56 -0.4 389,095 + B158DRAFT_2234 0.57 +0.3 389,103 - B158DRAFT_2234 0.58 +0.0 389,183 - B158DRAFT_2234 0.65 -0.6 389,297 - B158DRAFT_2234 0.74 -0.2 389,373 - B158DRAFT_2234 0.81 +0.1 389,390 + B158DRAFT_2234 0.82 +0.2 389,444 - B158DRAFT_2234 0.87 -0.2 389,484 - -0.6 389,576 + +0.2 389,586 + -0.8 389,586 + +0.3 389,678 + B158DRAFT_2235 0.13 +0.1 389,753 - B158DRAFT_2235 0.29 +0.2 389,805 - B158DRAFT_2235 0.39 +0.4 389,814 + B158DRAFT_2235 0.41 -0.3 389,838 - B158DRAFT_2235 0.46 +0.1 389,871 + B158DRAFT_2235 0.52 -0.0 389,871 + B158DRAFT_2235 0.52 -0.1 389,871 + B158DRAFT_2235 0.52 -0.0 389,871 + B158DRAFT_2235 0.52 -0.4 389,871 + B158DRAFT_2235 0.52 +1.4 389,871 + B158DRAFT_2235 0.52 -0.4 389,871 + B158DRAFT_2235 0.52 -1.1 389,871 + B158DRAFT_2235 0.52 +0.8 389,871 + B158DRAFT_2235 0.52 -0.4 389,871 + B158DRAFT_2235 0.52 -0.4 389,871 + B158DRAFT_2235 0.52 -0.1 389,871 + B158DRAFT_2235 0.52 +0.1 389,871 + B158DRAFT_2235 0.52 +0.3 389,871 + B158DRAFT_2235 0.52 +0.0 389,871 + B158DRAFT_2235 0.52 -1.2 389,879 - B158DRAFT_2235 0.54 -0.5 389,879 - B158DRAFT_2235 0.54 -0.1 389,879 - B158DRAFT_2235 0.54 -0.1 389,879 - B158DRAFT_2235 0.54 -0.3 389,879 - B158DRAFT_2235 0.54 +0.2 389,909 + B158DRAFT_2235 0.60 -0.9 389,959 + B158DRAFT_2235 0.70 -1.0 389,982 + B158DRAFT_2235 0.74 -0.4 389,988 + B158DRAFT_2235 0.76 -0.1 389,988 + B158DRAFT_2235 0.76 -0.1 389,998 + B158DRAFT_2235 0.78 -0.2 390,021 + B158DRAFT_2235 0.82 +0.2 390,026 + B158DRAFT_2235 0.83 -0.6 390,034 - B158DRAFT_2235 0.85 -0.1 390,098 + +0.2 390,098 + +0.4 390,106 - -0.2 390,225 + B158DRAFT_2236 0.13 +0.3 390,225 + B158DRAFT_2236 0.13 -0.2 390,233 - B158DRAFT_2236 0.15 +0.2 390,233 - B158DRAFT_2236 0.15 -0.4 390,233 - B158DRAFT_2236 0.15 -0.4 390,235 + B158DRAFT_2236 0.15 +0.4 390,235 + B158DRAFT_2236 0.15 +0.4 390,243 - B158DRAFT_2236 0.17 +0.4 390,243 - B158DRAFT_2236 0.17 +0.9 390,256 - B158DRAFT_2236 0.20 +0.6 390,266 - B158DRAFT_2236 0.22 +0.2 390,268 + B158DRAFT_2236 0.22 +0.4 390,285 + B158DRAFT_2236 0.26 +0.3 390,287 + B158DRAFT_2236 0.26 -0.4 390,287 + B158DRAFT_2236 0.26 +0.4 390,287 + B158DRAFT_2236 0.26 +0.1 390,298 + B158DRAFT_2236 0.29 +0.7 390,300 - B158DRAFT_2236 0.29 +0.4 390,302 + B158DRAFT_2236 0.30 +0.4 390,306 - B158DRAFT_2236 0.31 +0.2 390,319 + B158DRAFT_2236 0.33 -0.1 390,407 + B158DRAFT_2236 0.53 +0.4 390,447 + B158DRAFT_2236 0.61 +0.0 390,455 + B158DRAFT_2236 0.63 +0.6 390,455 + B158DRAFT_2236 0.63 +0.6 390,463 - B158DRAFT_2236 0.65 -0.1 390,493 - B158DRAFT_2236 0.71 +0.1
Or see this region's nucleotide sequence