Experiment: m.b. D-Cycloserine 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_2062 and B158DRAFT_2063 are separated by 70 nucleotides B158DRAFT_2063 and B158DRAFT_2064 are separated by 5 nucleotides
B158DRAFT_2062: B158DRAFT_2062 - glutamyl-tRNA reductase (EC 1.2.1.70), at 197,267 to 198,523
_2062
B158DRAFT_2063: B158DRAFT_2063 - peptide chain release factor 1, at 198,594 to 199,688
_2063
B158DRAFT_2064: B158DRAFT_2064 - [protein release factor]-glutamine N5-methyltransferase (EC 2.1.1.-), at 199,694 to 200,527
_2064
Position (kb)
198
199
200 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 198.539 kb on + strand at 198.545 kb on + strand at 198.547 kb on + strand at 198.560 kb on + strand at 199.717 kb on + strand at 199.717 kb on + strand at 199.717 kb on + strand at 199.718 kb on + strand at 199.725 kb on - strand at 199.725 kb on - strand at 199.726 kb on - strand at 199.726 kb on - strand at 199.726 kb on - strand at 199.749 kb on + strand at 199.761 kb on - strand at 199.812 kb on - strand, within B158DRAFT_2064 at 199.816 kb on + strand, within B158DRAFT_2064 at 199.832 kb on + strand, within B158DRAFT_2064 at 199.835 kb on - strand, within B158DRAFT_2064 at 199.899 kb on + strand, within B158DRAFT_2064 at 199.965 kb on + strand, within B158DRAFT_2064 at 199.973 kb on - strand, within B158DRAFT_2064 at 200.075 kb on + strand, within B158DRAFT_2064 at 200.083 kb on - strand, within B158DRAFT_2064 at 200.092 kb on + strand, within B158DRAFT_2064 at 200.092 kb on + strand, within B158DRAFT_2064 at 200.100 kb on - strand, within B158DRAFT_2064 at 200.103 kb on + strand, within B158DRAFT_2064 at 200.110 kb on + strand, within B158DRAFT_2064 at 200.110 kb on + strand, within B158DRAFT_2064 at 200.110 kb on + strand, within B158DRAFT_2064 at 200.118 kb on - strand, within B158DRAFT_2064 at 200.118 kb on - strand, within B158DRAFT_2064 at 200.118 kb on - strand, within B158DRAFT_2064 at 200.118 kb on - strand, within B158DRAFT_2064 at 200.118 kb on - strand, within B158DRAFT_2064 at 200.122 kb on + strand, within B158DRAFT_2064 at 200.130 kb on - strand, within B158DRAFT_2064 at 200.155 kb on + strand, within B158DRAFT_2064 at 200.155 kb on + strand, within B158DRAFT_2064 at 200.195 kb on + strand, within B158DRAFT_2064 at 200.195 kb on + strand, within B158DRAFT_2064 at 200.217 kb on + strand, within B158DRAFT_2064 at 200.244 kb on - strand, within B158DRAFT_2064 at 200.256 kb on - strand, within B158DRAFT_2064 at 200.299 kb on - strand, within B158DRAFT_2064 at 200.329 kb on + strand, within B158DRAFT_2064 at 200.337 kb on - strand, within B158DRAFT_2064 at 200.415 kb on + strand, within B158DRAFT_2064 at 200.415 kb on + strand, within B158DRAFT_2064 at 200.415 kb on + strand, within B158DRAFT_2064 at 200.420 kb on + strand, within B158DRAFT_2064 at 200.423 kb on - strand, within B158DRAFT_2064 at 200.423 kb on - strand, within B158DRAFT_2064 at 200.423 kb on - strand, within B158DRAFT_2064 at 200.423 kb on - strand, within B158DRAFT_2064 at 200.423 kb on - strand, within B158DRAFT_2064 at 200.425 kb on + strand, within B158DRAFT_2064 at 200.460 kb on + strand at 200.460 kb on + strand at 200.478 kb on - strand at 200.478 kb on - strand at 200.485 kb on + strand at 200.495 kb on + strand at 200.495 kb on + strand at 200.495 kb on + strand at 200.495 kb on + strand at 200.503 kb on - strand at 200.503 kb on - strand at 200.503 kb on - strand at 200.540 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. D-Cycloserine 0.03125 mg/ml remove 198,539 + -2.1 198,545 + -0.5 198,547 + +0.5 198,560 + +0.5 199,717 + -2.2 199,717 + -1.1 199,717 + -2.0 199,718 + -0.8 199,725 - -1.7 199,725 - -2.7 199,726 - -1.4 199,726 - -1.5 199,726 - -1.5 199,749 + -2.0 199,761 - -1.1 199,812 - B158DRAFT_2064 0.14 -2.0 199,816 + B158DRAFT_2064 0.15 -3.7 199,832 + B158DRAFT_2064 0.17 -2.7 199,835 - B158DRAFT_2064 0.17 -1.4 199,899 + B158DRAFT_2064 0.25 -1.3 199,965 + B158DRAFT_2064 0.32 -2.2 199,973 - B158DRAFT_2064 0.33 -2.0 200,075 + B158DRAFT_2064 0.46 -2.5 200,083 - B158DRAFT_2064 0.47 -1.4 200,092 + B158DRAFT_2064 0.48 -2.6 200,092 + B158DRAFT_2064 0.48 -2.6 200,100 - B158DRAFT_2064 0.49 -2.2 200,103 + B158DRAFT_2064 0.49 -1.9 200,110 + B158DRAFT_2064 0.50 -1.5 200,110 + B158DRAFT_2064 0.50 -1.8 200,110 + B158DRAFT_2064 0.50 -1.4 200,118 - B158DRAFT_2064 0.51 -2.3 200,118 - B158DRAFT_2064 0.51 -1.8 200,118 - B158DRAFT_2064 0.51 -1.3 200,118 - B158DRAFT_2064 0.51 -2.8 200,118 - B158DRAFT_2064 0.51 -1.5 200,122 + B158DRAFT_2064 0.51 -2.2 200,130 - B158DRAFT_2064 0.52 -1.9 200,155 + B158DRAFT_2064 0.55 -2.7 200,155 + B158DRAFT_2064 0.55 -2.2 200,195 + B158DRAFT_2064 0.60 -1.5 200,195 + B158DRAFT_2064 0.60 -1.2 200,217 + B158DRAFT_2064 0.63 -2.8 200,244 - B158DRAFT_2064 0.66 -1.0 200,256 - B158DRAFT_2064 0.67 -1.9 200,299 - B158DRAFT_2064 0.73 -2.5 200,329 + B158DRAFT_2064 0.76 -2.1 200,337 - B158DRAFT_2064 0.77 -1.1 200,415 + B158DRAFT_2064 0.86 -2.3 200,415 + B158DRAFT_2064 0.86 -1.6 200,415 + B158DRAFT_2064 0.86 -2.7 200,420 + B158DRAFT_2064 0.87 -1.5 200,423 - B158DRAFT_2064 0.87 -2.2 200,423 - B158DRAFT_2064 0.87 -2.1 200,423 - B158DRAFT_2064 0.87 -1.7 200,423 - B158DRAFT_2064 0.87 -2.6 200,423 - B158DRAFT_2064 0.87 -2.8 200,425 + B158DRAFT_2064 0.88 -2.2 200,460 + -1.8 200,460 + -3.6 200,478 - -2.9 200,478 - -2.0 200,485 + -1.1 200,495 + -2.7 200,495 + -1.6 200,495 + -1.7 200,495 + -1.3 200,503 - -2.2 200,503 - -1.5 200,503 - -2.2 200,540 - -0.7
Or see this region's nucleotide sequence