Experiment: m.b. Nickel (II) chloride 1.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_2181 and B158DRAFT_2182 are separated by 149 nucleotides B158DRAFT_2182 and B158DRAFT_2183 are separated by 154 nucleotides B158DRAFT_2183 and B158DRAFT_2184 are separated by 179 nucleotides
B158DRAFT_2181: B158DRAFT_2181 - Negative regulator of sigma E activity, at 328,361 to 329,041
_2181
B158DRAFT_2182: B158DRAFT_2182 - positive regulator of sigma(E), RseC/MucC, at 329,191 to 329,682
_2182
B158DRAFT_2183: B158DRAFT_2183 - periplasmic serine protease, Do/DeqQ family, at 329,837 to 331,138
_2183
B158DRAFT_2184: B158DRAFT_2184 - GTP-binding protein LepA, at 331,318 to 333,126
_2184
Position (kb)
329
330
331
332 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 328.947 kb on - strand, within B158DRAFT_2181 at 328.981 kb on + strand at 329.083 kb on + strand at 329.201 kb on + strand at 329.561 kb on + strand, within B158DRAFT_2182 at 329.596 kb on + strand, within B158DRAFT_2182 at 329.671 kb on + strand at 329.691 kb on - strand at 329.839 kb on - strand at 329.864 kb on + strand at 329.967 kb on - strand at 329.980 kb on + strand, within B158DRAFT_2183 at 329.988 kb on - strand, within B158DRAFT_2183 at 329.988 kb on - strand, within B158DRAFT_2183 at 329.988 kb on - strand, within B158DRAFT_2183 at 330.011 kb on - strand, within B158DRAFT_2183 at 330.016 kb on + strand, within B158DRAFT_2183 at 330.031 kb on - strand, within B158DRAFT_2183 at 330.031 kb on - strand, within B158DRAFT_2183 at 330.032 kb on - strand, within B158DRAFT_2183 at 330.099 kb on + strand, within B158DRAFT_2183 at 330.107 kb on - strand, within B158DRAFT_2183 at 330.124 kb on + strand, within B158DRAFT_2183 at 330.124 kb on + strand, within B158DRAFT_2183 at 330.132 kb on - strand, within B158DRAFT_2183 at 330.132 kb on - strand, within B158DRAFT_2183 at 330.137 kb on - strand, within B158DRAFT_2183 at 330.144 kb on - strand, within B158DRAFT_2183 at 330.147 kb on + strand, within B158DRAFT_2183 at 330.162 kb on + strand, within B158DRAFT_2183 at 330.207 kb on + strand, within B158DRAFT_2183 at 330.207 kb on + strand, within B158DRAFT_2183 at 330.231 kb on + strand, within B158DRAFT_2183 at 330.304 kb on + strand, within B158DRAFT_2183 at 330.307 kb on - strand, within B158DRAFT_2183 at 330.309 kb on + strand, within B158DRAFT_2183 at 330.317 kb on - strand, within B158DRAFT_2183 at 330.317 kb on - strand, within B158DRAFT_2183 at 330.317 kb on - strand, within B158DRAFT_2183 at 330.317 kb on - strand, within B158DRAFT_2183 at 330.393 kb on - strand, within B158DRAFT_2183 at 330.395 kb on + strand, within B158DRAFT_2183 at 330.413 kb on - strand, within B158DRAFT_2183 at 330.413 kb on - strand, within B158DRAFT_2183 at 330.532 kb on + strand, within B158DRAFT_2183 at 330.546 kb on + strand, within B158DRAFT_2183 at 330.555 kb on - strand, within B158DRAFT_2183 at 330.572 kb on + strand, within B158DRAFT_2183 at 330.572 kb on + strand, within B158DRAFT_2183 at 330.572 kb on + strand, within B158DRAFT_2183 at 330.577 kb on + strand, within B158DRAFT_2183 at 330.577 kb on + strand, within B158DRAFT_2183 at 330.577 kb on + strand, within B158DRAFT_2183 at 330.577 kb on + strand, within B158DRAFT_2183 at 330.580 kb on - strand, within B158DRAFT_2183 at 330.580 kb on - strand, within B158DRAFT_2183 at 330.580 kb on - strand, within B158DRAFT_2183 at 330.580 kb on - strand, within B158DRAFT_2183 at 330.585 kb on - strand, within B158DRAFT_2183 at 330.590 kb on - strand, within B158DRAFT_2183 at 330.635 kb on - strand, within B158DRAFT_2183 at 330.662 kb on + strand, within B158DRAFT_2183 at 330.662 kb on + strand, within B158DRAFT_2183 at 330.663 kb on + strand, within B158DRAFT_2183 at 330.663 kb on + strand, within B158DRAFT_2183 at 330.670 kb on + strand, within B158DRAFT_2183 at 330.671 kb on - strand, within B158DRAFT_2183 at 330.671 kb on - strand, within B158DRAFT_2183 at 330.671 kb on - strand, within B158DRAFT_2183 at 330.697 kb on + strand, within B158DRAFT_2183 at 330.697 kb on + strand, within B158DRAFT_2183 at 330.697 kb on + strand, within B158DRAFT_2183 at 330.697 kb on + strand, within B158DRAFT_2183 at 330.697 kb on + strand, within B158DRAFT_2183 at 330.697 kb on + strand, within B158DRAFT_2183 at 330.705 kb on - strand, within B158DRAFT_2183 at 330.705 kb on - strand, within B158DRAFT_2183 at 330.716 kb on + strand, within B158DRAFT_2183 at 330.716 kb on + strand, within B158DRAFT_2183 at 330.721 kb on + strand, within B158DRAFT_2183 at 330.724 kb on - strand, within B158DRAFT_2183 at 330.729 kb on - strand, within B158DRAFT_2183 at 330.812 kb on + strand, within B158DRAFT_2183 at 330.854 kb on + strand, within B158DRAFT_2183 at 330.854 kb on + strand, within B158DRAFT_2183 at 330.862 kb on - strand, within B158DRAFT_2183 at 330.919 kb on + strand, within B158DRAFT_2183 at 330.927 kb on + strand, within B158DRAFT_2183 at 330.950 kb on + strand, within B158DRAFT_2183 at 331.034 kb on - strand at 331.092 kb on - strand at 331.093 kb on - strand at 331.099 kb on + strand at 331.102 kb on - strand at 331.107 kb on - strand at 331.107 kb on - strand at 331.133 kb on + strand at 331.141 kb on - strand at 331.167 kb on + strand at 331.167 kb on + strand at 331.167 kb on + strand at 331.172 kb on + strand at 331.175 kb on - strand at 331.175 kb on - strand at 331.243 kb on + strand at 331.297 kb on + strand at 331.297 kb on + strand at 331.313 kb on + strand at 331.315 kb on + strand at 331.320 kb on + strand at 331.333 kb on - strand at 331.404 kb on + strand at 331.414 kb on + strand at 331.459 kb on + strand at 331.469 kb on - strand at 331.497 kb on + strand at 331.499 kb on + strand, within B158DRAFT_2184 at 331.499 kb on + strand, within B158DRAFT_2184 at 331.504 kb on + strand, within B158DRAFT_2184 at 331.507 kb on - strand, within B158DRAFT_2184 at 331.540 kb on + strand, within B158DRAFT_2184 at 331.559 kb on + strand, within B158DRAFT_2184 at 331.559 kb on + strand, within B158DRAFT_2184 at 331.654 kb on + strand, within B158DRAFT_2184 at 331.741 kb on + strand, within B158DRAFT_2184 at 331.801 kb on + strand, within B158DRAFT_2184 at 331.801 kb on + strand, within B158DRAFT_2184 at 331.801 kb on + strand, within B158DRAFT_2184 at 331.872 kb on + strand, within B158DRAFT_2184 at 331.872 kb on + strand, within B158DRAFT_2184 at 331.894 kb on + strand, within B158DRAFT_2184 at 331.894 kb on + strand, within B158DRAFT_2184 at 332.118 kb on + strand, within B158DRAFT_2184 at 332.124 kb on + strand, within B158DRAFT_2184 at 332.134 kb on + strand, within B158DRAFT_2184
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Nickel (II) chloride 1.25 mM remove 328,947 - B158DRAFT_2181 0.86 +0.8 328,981 + -0.2 329,083 + +0.0 329,201 + +0.2 329,561 + B158DRAFT_2182 0.75 -2.6 329,596 + B158DRAFT_2182 0.82 -0.2 329,671 + -0.4 329,691 - -0.8 329,839 - +0.1 329,864 + -0.9 329,967 - +0.2 329,980 + B158DRAFT_2183 0.11 -1.1 329,988 - B158DRAFT_2183 0.12 +1.0 329,988 - B158DRAFT_2183 0.12 -0.0 329,988 - B158DRAFT_2183 0.12 -0.1 330,011 - B158DRAFT_2183 0.13 -0.5 330,016 + B158DRAFT_2183 0.14 -0.6 330,031 - B158DRAFT_2183 0.15 -0.2 330,031 - B158DRAFT_2183 0.15 +0.6 330,032 - B158DRAFT_2183 0.15 -0.0 330,099 + B158DRAFT_2183 0.20 +1.1 330,107 - B158DRAFT_2183 0.21 -0.6 330,124 + B158DRAFT_2183 0.22 +0.3 330,124 + B158DRAFT_2183 0.22 -0.2 330,132 - B158DRAFT_2183 0.23 +0.1 330,132 - B158DRAFT_2183 0.23 -0.4 330,137 - B158DRAFT_2183 0.23 -1.6 330,144 - B158DRAFT_2183 0.24 -0.5 330,147 + B158DRAFT_2183 0.24 -1.9 330,162 + B158DRAFT_2183 0.25 -0.1 330,207 + B158DRAFT_2183 0.28 -0.2 330,207 + B158DRAFT_2183 0.28 -0.4 330,231 + B158DRAFT_2183 0.30 -0.6 330,304 + B158DRAFT_2183 0.36 -0.1 330,307 - B158DRAFT_2183 0.36 -0.2 330,309 + B158DRAFT_2183 0.36 -0.6 330,317 - B158DRAFT_2183 0.37 -0.2 330,317 - B158DRAFT_2183 0.37 +0.4 330,317 - B158DRAFT_2183 0.37 -0.1 330,317 - B158DRAFT_2183 0.37 -0.2 330,393 - B158DRAFT_2183 0.43 -0.2 330,395 + B158DRAFT_2183 0.43 +0.0 330,413 - B158DRAFT_2183 0.44 -0.0 330,413 - B158DRAFT_2183 0.44 +0.0 330,532 + B158DRAFT_2183 0.53 -0.4 330,546 + B158DRAFT_2183 0.54 +0.1 330,555 - B158DRAFT_2183 0.55 -0.6 330,572 + B158DRAFT_2183 0.56 -0.2 330,572 + B158DRAFT_2183 0.56 +0.3 330,572 + B158DRAFT_2183 0.56 -0.7 330,577 + B158DRAFT_2183 0.57 -0.8 330,577 + B158DRAFT_2183 0.57 -0.2 330,577 + B158DRAFT_2183 0.57 +0.0 330,577 + B158DRAFT_2183 0.57 +0.3 330,580 - B158DRAFT_2183 0.57 -0.3 330,580 - B158DRAFT_2183 0.57 -0.0 330,580 - B158DRAFT_2183 0.57 -0.2 330,580 - B158DRAFT_2183 0.57 -0.4 330,585 - B158DRAFT_2183 0.57 -0.1 330,590 - B158DRAFT_2183 0.58 -1.2 330,635 - B158DRAFT_2183 0.61 +1.1 330,662 + B158DRAFT_2183 0.63 -0.3 330,662 + B158DRAFT_2183 0.63 -0.2 330,663 + B158DRAFT_2183 0.63 -0.2 330,663 + B158DRAFT_2183 0.63 +0.3 330,670 + B158DRAFT_2183 0.64 +0.0 330,671 - B158DRAFT_2183 0.64 -0.2 330,671 - B158DRAFT_2183 0.64 +0.0 330,671 - B158DRAFT_2183 0.64 +0.3 330,697 + B158DRAFT_2183 0.66 -0.6 330,697 + B158DRAFT_2183 0.66 -0.9 330,697 + B158DRAFT_2183 0.66 -0.1 330,697 + B158DRAFT_2183 0.66 -0.6 330,697 + B158DRAFT_2183 0.66 -0.2 330,697 + B158DRAFT_2183 0.66 -0.4 330,705 - B158DRAFT_2183 0.67 -0.2 330,705 - B158DRAFT_2183 0.67 +0.1 330,716 + B158DRAFT_2183 0.68 -0.4 330,716 + B158DRAFT_2183 0.68 -0.2 330,721 + B158DRAFT_2183 0.68 -0.1 330,724 - B158DRAFT_2183 0.68 -0.8 330,729 - B158DRAFT_2183 0.69 -0.5 330,812 + B158DRAFT_2183 0.75 +0.8 330,854 + B158DRAFT_2183 0.78 -1.1 330,854 + B158DRAFT_2183 0.78 -0.2 330,862 - B158DRAFT_2183 0.79 -0.5 330,919 + B158DRAFT_2183 0.83 -0.5 330,927 + B158DRAFT_2183 0.84 -0.9 330,950 + B158DRAFT_2183 0.85 -1.0 331,034 - -0.9 331,092 - -0.5 331,093 - -0.3 331,099 + -0.6 331,102 - +0.1 331,107 - +0.1 331,107 - -0.8 331,133 + +0.0 331,141 - -0.5 331,167 + +0.1 331,167 + +0.2 331,167 + -0.1 331,172 + +0.4 331,175 - -0.4 331,175 - +0.5 331,243 + +0.8 331,297 + +0.5 331,297 + -0.4 331,313 + +1.2 331,315 + -1.2 331,320 + -2.4 331,333 - -3.0 331,404 + -1.0 331,414 + -0.6 331,459 + -0.2 331,469 - -2.0 331,497 + -0.4 331,499 + B158DRAFT_2184 0.10 +0.8 331,499 + B158DRAFT_2184 0.10 -1.2 331,504 + B158DRAFT_2184 0.10 -0.8 331,507 - B158DRAFT_2184 0.10 -2.0 331,540 + B158DRAFT_2184 0.12 -0.5 331,559 + B158DRAFT_2184 0.13 -0.1 331,559 + B158DRAFT_2184 0.13 -2.4 331,654 + B158DRAFT_2184 0.19 +0.8 331,741 + B158DRAFT_2184 0.23 +0.9 331,801 + B158DRAFT_2184 0.27 -0.3 331,801 + B158DRAFT_2184 0.27 +0.5 331,801 + B158DRAFT_2184 0.27 +1.0 331,872 + B158DRAFT_2184 0.31 +0.0 331,872 + B158DRAFT_2184 0.31 -0.7 331,894 + B158DRAFT_2184 0.32 -0.6 331,894 + B158DRAFT_2184 0.32 -0.3 332,118 + B158DRAFT_2184 0.44 +0.3 332,124 + B158DRAFT_2184 0.45 +0.4 332,134 + B158DRAFT_2184 0.45 +0.2
Or see this region's nucleotide sequence