Experiment: m.b. Nickel (II) chloride 1.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_2024 and B158DRAFT_2025 are separated by 76 nucleotides B158DRAFT_2025 and B158DRAFT_2026 are separated by 41 nucleotides B158DRAFT_2026 and B158DRAFT_2027 are separated by 498 nucleotides
B158DRAFT_2024: B158DRAFT_2024 - 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway), at 154,243 to 154,977
_2024
B158DRAFT_2025: B158DRAFT_2025 - Uncharacterized protein conserved in bacteria, at 155,054 to 155,488
_2025
B158DRAFT_2026: B158DRAFT_2026 - Uncharacterized protein conserved in bacteria, at 155,530 to 156,549
_2026
B158DRAFT_2027: B158DRAFT_2027 - RNA polymerase sigma factor, sigma-70 family, at 157,048 to 157,572
_2027
Position (kb)
155
156
157 Strain fitness (log2 ratio)
-1
0
1 at 154.545 kb on + strand, within B158DRAFT_2024 at 154.597 kb on - strand, within B158DRAFT_2024 at 154.599 kb on + strand, within B158DRAFT_2024 at 154.599 kb on + strand, within B158DRAFT_2024 at 154.599 kb on + strand, within B158DRAFT_2024 at 154.599 kb on + strand, within B158DRAFT_2024 at 154.599 kb on + strand, within B158DRAFT_2024 at 154.599 kb on + strand, within B158DRAFT_2024 at 154.605 kb on + strand, within B158DRAFT_2024 at 154.607 kb on - strand, within B158DRAFT_2024 at 154.607 kb on - strand, within B158DRAFT_2024 at 154.607 kb on - strand, within B158DRAFT_2024 at 154.640 kb on - strand, within B158DRAFT_2024 at 154.649 kb on - strand, within B158DRAFT_2024 at 154.733 kb on - strand, within B158DRAFT_2024 at 154.748 kb on + strand, within B158DRAFT_2024 at 154.784 kb on + strand, within B158DRAFT_2024 at 154.794 kb on + strand, within B158DRAFT_2024 at 154.802 kb on - strand, within B158DRAFT_2024 at 154.802 kb on - strand, within B158DRAFT_2024 at 154.802 kb on - strand, within B158DRAFT_2024 at 154.803 kb on - strand, within B158DRAFT_2024 at 154.813 kb on + strand, within B158DRAFT_2024 at 154.813 kb on + strand, within B158DRAFT_2024 at 154.821 kb on - strand, within B158DRAFT_2024 at 154.828 kb on + strand, within B158DRAFT_2024 at 154.831 kb on - strand, within B158DRAFT_2024 at 154.841 kb on + strand, within B158DRAFT_2024 at 154.932 kb on + strand at 155.000 kb on - strand at 155.010 kb on + strand at 155.187 kb on - strand, within B158DRAFT_2025 at 155.256 kb on + strand, within B158DRAFT_2025 at 155.256 kb on + strand, within B158DRAFT_2025 at 155.256 kb on + strand, within B158DRAFT_2025 at 155.280 kb on + strand, within B158DRAFT_2025 at 155.310 kb on - strand, within B158DRAFT_2025 at 155.385 kb on + strand, within B158DRAFT_2025 at 155.385 kb on + strand, within B158DRAFT_2025 at 155.385 kb on + strand, within B158DRAFT_2025 at 155.393 kb on - strand, within B158DRAFT_2025 at 155.393 kb on - strand, within B158DRAFT_2025 at 155.397 kb on + strand, within B158DRAFT_2025 at 155.408 kb on + strand, within B158DRAFT_2025 at 155.415 kb on - strand, within B158DRAFT_2025 at 155.463 kb on + strand at 155.492 kb on - strand at 155.502 kb on - strand at 155.517 kb on - strand at 155.517 kb on - strand at 155.532 kb on + strand at 155.573 kb on + strand at 155.581 kb on - strand at 155.677 kb on + strand, within B158DRAFT_2026 at 155.687 kb on + strand, within B158DRAFT_2026 at 155.687 kb on + strand, within B158DRAFT_2026 at 155.687 kb on + strand, within B158DRAFT_2026 at 155.695 kb on - strand, within B158DRAFT_2026 at 155.695 kb on - strand, within B158DRAFT_2026 at 155.893 kb on + strand, within B158DRAFT_2026 at 155.894 kb on - strand, within B158DRAFT_2026 at 155.918 kb on - strand, within B158DRAFT_2026 at 156.013 kb on + strand, within B158DRAFT_2026 at 156.031 kb on - strand, within B158DRAFT_2026 at 156.115 kb on - strand, within B158DRAFT_2026 at 156.151 kb on - strand, within B158DRAFT_2026 at 156.169 kb on - strand, within B158DRAFT_2026 at 156.187 kb on + strand, within B158DRAFT_2026 at 156.192 kb on + strand, within B158DRAFT_2026 at 156.195 kb on - strand, within B158DRAFT_2026 at 156.203 kb on + strand, within B158DRAFT_2026 at 156.211 kb on - strand, within B158DRAFT_2026 at 156.213 kb on + strand, within B158DRAFT_2026 at 156.213 kb on + strand, within B158DRAFT_2026 at 156.213 kb on - strand, within B158DRAFT_2026 at 156.225 kb on - strand, within B158DRAFT_2026 at 156.240 kb on + strand, within B158DRAFT_2026 at 156.251 kb on + strand, within B158DRAFT_2026 at 156.252 kb on - strand, within B158DRAFT_2026 at 156.296 kb on + strand, within B158DRAFT_2026 at 156.408 kb on + strand, within B158DRAFT_2026 at 156.477 kb on + strand at 156.483 kb on - strand at 156.485 kb on - strand at 156.542 kb on - strand at 156.663 kb on - strand at 156.696 kb on + strand at 156.704 kb on + strand at 156.704 kb on + strand at 156.704 kb on + strand at 156.704 kb on + strand at 156.704 kb on + strand at 156.704 kb on + strand at 156.704 kb on + strand at 156.712 kb on - strand at 156.712 kb on - strand at 156.712 kb on - strand at 156.712 kb on - strand at 156.712 kb on - strand at 156.734 kb on + strand at 156.748 kb on + strand at 156.756 kb on + strand at 156.756 kb on + strand at 156.764 kb on - strand at 156.804 kb on + strand at 156.816 kb on + strand at 156.826 kb on + strand at 156.826 kb on + strand at 156.931 kb on + strand at 156.939 kb on - strand at 157.005 kb on - strand at 157.007 kb on - strand at 157.022 kb on - strand at 157.038 kb on - strand at 157.043 kb on - strand at 157.215 kb on + strand, within B158DRAFT_2027 at 157.295 kb on + strand, within B158DRAFT_2027 at 157.295 kb on + strand, within B158DRAFT_2027 at 157.368 kb on + strand, within B158DRAFT_2027 at 157.431 kb on + strand, within B158DRAFT_2027 at 157.439 kb on - strand, within B158DRAFT_2027 at 157.439 kb on - strand, within B158DRAFT_2027 at 157.460 kb on - strand, within B158DRAFT_2027 at 157.503 kb on + strand, within B158DRAFT_2027 at 157.515 kb on + strand, within B158DRAFT_2027 at 157.515 kb on + strand, within B158DRAFT_2027 at 157.520 kb on + strand at 157.523 kb on - strand at 157.525 kb on + strand at 157.525 kb on + strand at 157.525 kb on + strand at 157.533 kb on - strand at 157.543 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Nickel (II) chloride 1.25 mM remove 154,545 + B158DRAFT_2024 0.41 -0.3 154,597 - B158DRAFT_2024 0.48 -0.2 154,599 + B158DRAFT_2024 0.48 -0.1 154,599 + B158DRAFT_2024 0.48 +0.1 154,599 + B158DRAFT_2024 0.48 +0.6 154,599 + B158DRAFT_2024 0.48 -1.2 154,599 + B158DRAFT_2024 0.48 -0.4 154,599 + B158DRAFT_2024 0.48 +0.8 154,605 + B158DRAFT_2024 0.49 -0.7 154,607 - B158DRAFT_2024 0.50 +0.1 154,607 - B158DRAFT_2024 0.50 +0.3 154,607 - B158DRAFT_2024 0.50 +0.3 154,640 - B158DRAFT_2024 0.54 -0.4 154,649 - B158DRAFT_2024 0.55 -0.3 154,733 - B158DRAFT_2024 0.67 -0.6 154,748 + B158DRAFT_2024 0.69 -0.8 154,784 + B158DRAFT_2024 0.74 +0.7 154,794 + B158DRAFT_2024 0.75 +0.1 154,802 - B158DRAFT_2024 0.76 -0.7 154,802 - B158DRAFT_2024 0.76 -0.3 154,802 - B158DRAFT_2024 0.76 -0.5 154,803 - B158DRAFT_2024 0.76 -0.6 154,813 + B158DRAFT_2024 0.78 -0.2 154,813 + B158DRAFT_2024 0.78 -0.5 154,821 - B158DRAFT_2024 0.79 -0.3 154,828 + B158DRAFT_2024 0.80 +0.2 154,831 - B158DRAFT_2024 0.80 -0.2 154,841 + B158DRAFT_2024 0.81 -0.2 154,932 + -0.5 155,000 - -0.6 155,010 + +1.2 155,187 - B158DRAFT_2025 0.31 -0.9 155,256 + B158DRAFT_2025 0.46 -0.7 155,256 + B158DRAFT_2025 0.46 -0.5 155,256 + B158DRAFT_2025 0.46 -0.7 155,280 + B158DRAFT_2025 0.52 -1.2 155,310 - B158DRAFT_2025 0.59 -0.5 155,385 + B158DRAFT_2025 0.76 -1.1 155,385 + B158DRAFT_2025 0.76 -0.4 155,385 + B158DRAFT_2025 0.76 -1.2 155,393 - B158DRAFT_2025 0.78 -1.0 155,393 - B158DRAFT_2025 0.78 -0.6 155,397 + B158DRAFT_2025 0.79 -1.0 155,408 + B158DRAFT_2025 0.81 -0.0 155,415 - B158DRAFT_2025 0.83 -0.8 155,463 + +0.7 155,492 - +0.1 155,502 - -1.4 155,517 - +0.2 155,517 - -0.1 155,532 + -0.2 155,573 + -0.3 155,581 - -1.3 155,677 + B158DRAFT_2026 0.14 +0.1 155,687 + B158DRAFT_2026 0.15 +0.5 155,687 + B158DRAFT_2026 0.15 -0.2 155,687 + B158DRAFT_2026 0.15 -0.7 155,695 - B158DRAFT_2026 0.16 -0.7 155,695 - B158DRAFT_2026 0.16 -1.2 155,893 + B158DRAFT_2026 0.36 -0.3 155,894 - B158DRAFT_2026 0.36 -1.6 155,918 - B158DRAFT_2026 0.38 -0.2 156,013 + B158DRAFT_2026 0.47 -1.6 156,031 - B158DRAFT_2026 0.49 -0.5 156,115 - B158DRAFT_2026 0.57 -0.5 156,151 - B158DRAFT_2026 0.61 +0.2 156,169 - B158DRAFT_2026 0.63 -0.5 156,187 + B158DRAFT_2026 0.64 -0.4 156,192 + B158DRAFT_2026 0.65 +0.2 156,195 - B158DRAFT_2026 0.65 -0.0 156,203 + B158DRAFT_2026 0.66 -1.1 156,211 - B158DRAFT_2026 0.67 +0.6 156,213 + B158DRAFT_2026 0.67 +0.1 156,213 + B158DRAFT_2026 0.67 -0.8 156,213 - B158DRAFT_2026 0.67 +0.6 156,225 - B158DRAFT_2026 0.68 +0.1 156,240 + B158DRAFT_2026 0.70 -0.6 156,251 + B158DRAFT_2026 0.71 +0.8 156,252 - B158DRAFT_2026 0.71 +0.4 156,296 + B158DRAFT_2026 0.75 -0.3 156,408 + B158DRAFT_2026 0.86 +0.5 156,477 + -0.2 156,483 - -0.3 156,485 - +1.0 156,542 - -0.0 156,663 - -0.1 156,696 + +0.1 156,704 + -0.2 156,704 + +0.4 156,704 + +0.2 156,704 + -0.4 156,704 + -1.0 156,704 + +0.3 156,704 + -0.5 156,712 - +0.6 156,712 - +0.3 156,712 - -0.1 156,712 - -0.4 156,712 - +0.2 156,734 + -0.4 156,748 + +0.4 156,756 + -0.4 156,756 + -0.4 156,764 - -1.2 156,804 + -0.4 156,816 + +0.1 156,826 + +0.1 156,826 + -0.1 156,931 + +0.3 156,939 - +0.1 157,005 - -0.5 157,007 - -1.1 157,022 - -0.8 157,038 - -0.8 157,043 - -1.0 157,215 + B158DRAFT_2027 0.32 -0.4 157,295 + B158DRAFT_2027 0.47 +0.2 157,295 + B158DRAFT_2027 0.47 +0.2 157,368 + B158DRAFT_2027 0.61 -0.4 157,431 + B158DRAFT_2027 0.73 -0.4 157,439 - B158DRAFT_2027 0.74 -0.4 157,439 - B158DRAFT_2027 0.74 +0.1 157,460 - B158DRAFT_2027 0.78 +0.4 157,503 + B158DRAFT_2027 0.87 +0.3 157,515 + B158DRAFT_2027 0.89 -0.4 157,515 + B158DRAFT_2027 0.89 -0.4 157,520 + -1.7 157,523 - -0.3 157,525 + -0.1 157,525 + -0.2 157,525 + -0.7 157,533 - -0.4 157,543 - -0.5
Or see this region's nucleotide sequence