Experiment: m.b. Nickel (II) chloride 1.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_1820 and B158DRAFT_1821 are separated by 550 nucleotides B158DRAFT_1821 and B158DRAFT_1822 are separated by 92 nucleotides B158DRAFT_1822 and B158DRAFT_1823 are separated by 141 nucleotides
B158DRAFT_1820: B158DRAFT_1820 - periplasmic chaperone LolA, at 1,918,474 to 1,919,157
_1820
B158DRAFT_1821: B158DRAFT_1821 - DNA segregation ATPase FtsK/SpoIIIE and related proteins, at 1,919,708 to 1,922,047
_1821
B158DRAFT_1822: B158DRAFT_1822 - Transcriptional regulators, at 1,922,140 to 1,922,637
_1822
B158DRAFT_1823: B158DRAFT_1823 - alanine dehydrogenase, at 1,922,779 to 1,923,912
_1823
Position (kb)
1919
1920
1921
1922
1923 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1919.189 kb on + strand at 1919.189 kb on + strand at 1919.189 kb on + strand at 1919.189 kb on + strand at 1919.197 kb on - strand at 1919.197 kb on - strand at 1919.207 kb on - strand at 1919.237 kb on + strand at 1919.263 kb on + strand at 1919.263 kb on + strand at 1919.271 kb on - strand at 1919.271 kb on - strand at 1919.403 kb on + strand at 1919.419 kb on + strand at 1919.440 kb on + strand at 1919.448 kb on - strand at 1919.474 kb on + strand at 1919.482 kb on - strand at 1919.646 kb on + strand at 1919.719 kb on - strand at 1919.738 kb on - strand at 1919.738 kb on - strand at 1919.748 kb on + strand at 1919.752 kb on - strand at 1919.754 kb on - strand at 1919.795 kb on + strand at 1919.813 kb on + strand at 1919.859 kb on + strand at 1919.867 kb on - strand at 1919.915 kb on + strand at 1919.923 kb on - strand at 1919.923 kb on - strand at 1919.953 kb on - strand, within B158DRAFT_1821 at 1919.980 kb on - strand, within B158DRAFT_1821 at 1920.077 kb on - strand, within B158DRAFT_1821 at 1920.080 kb on + strand, within B158DRAFT_1821 at 1920.083 kb on - strand, within B158DRAFT_1821 at 1920.096 kb on + strand, within B158DRAFT_1821 at 1920.098 kb on + strand, within B158DRAFT_1821 at 1920.106 kb on - strand, within B158DRAFT_1821 at 1920.106 kb on - strand, within B158DRAFT_1821 at 1920.135 kb on - strand, within B158DRAFT_1821 at 1920.142 kb on + strand, within B158DRAFT_1821 at 1920.153 kb on + strand, within B158DRAFT_1821 at 1920.212 kb on + strand, within B158DRAFT_1821 at 1920.220 kb on - strand, within B158DRAFT_1821 at 1920.360 kb on - strand, within B158DRAFT_1821 at 1920.390 kb on + strand, within B158DRAFT_1821 at 1920.549 kb on + strand, within B158DRAFT_1821 at 1920.549 kb on + strand, within B158DRAFT_1821 at 1920.549 kb on + strand, within B158DRAFT_1821 at 1920.557 kb on - strand, within B158DRAFT_1821 at 1920.562 kb on - strand, within B158DRAFT_1821 at 1920.592 kb on - strand, within B158DRAFT_1821 at 1920.625 kb on - strand, within B158DRAFT_1821 at 1920.640 kb on + strand, within B158DRAFT_1821 at 1920.689 kb on - strand, within B158DRAFT_1821 at 1920.717 kb on + strand, within B158DRAFT_1821 at 1920.738 kb on + strand, within B158DRAFT_1821 at 1920.740 kb on + strand, within B158DRAFT_1821 at 1920.803 kb on + strand, within B158DRAFT_1821 at 1920.827 kb on - strand, within B158DRAFT_1821 at 1920.839 kb on - strand, within B158DRAFT_1821 at 1920.857 kb on + strand, within B158DRAFT_1821 at 1920.857 kb on + strand, within B158DRAFT_1821 at 1920.857 kb on + strand, within B158DRAFT_1821 at 1920.857 kb on + strand, within B158DRAFT_1821 at 1920.865 kb on - strand, within B158DRAFT_1821 at 1920.865 kb on - strand, within B158DRAFT_1821 at 1920.865 kb on - strand, within B158DRAFT_1821 at 1920.865 kb on - strand, within B158DRAFT_1821 at 1920.865 kb on - strand, within B158DRAFT_1821 at 1920.865 kb on - strand, within B158DRAFT_1821 at 1920.935 kb on + strand, within B158DRAFT_1821 at 1921.073 kb on + strand, within B158DRAFT_1821 at 1921.076 kb on - strand, within B158DRAFT_1821 at 1921.076 kb on - strand, within B158DRAFT_1821 at 1921.117 kb on + strand, within B158DRAFT_1821 at 1921.173 kb on - strand, within B158DRAFT_1821 at 1921.251 kb on + strand, within B158DRAFT_1821 at 1921.259 kb on - strand, within B158DRAFT_1821 at 1921.259 kb on - strand, within B158DRAFT_1821 at 1921.263 kb on + strand, within B158DRAFT_1821 at 1921.263 kb on + strand, within B158DRAFT_1821 at 1921.263 kb on + strand, within B158DRAFT_1821 at 1921.271 kb on - strand, within B158DRAFT_1821 at 1921.271 kb on - strand, within B158DRAFT_1821 at 1921.271 kb on - strand, within B158DRAFT_1821 at 1921.279 kb on + strand, within B158DRAFT_1821 at 1921.293 kb on - strand, within B158DRAFT_1821 at 1921.298 kb on - strand, within B158DRAFT_1821 at 1921.322 kb on - strand, within B158DRAFT_1821 at 1921.333 kb on - strand, within B158DRAFT_1821 at 1921.340 kb on + strand, within B158DRAFT_1821 at 1921.348 kb on - strand, within B158DRAFT_1821 at 1921.391 kb on - strand, within B158DRAFT_1821 at 1921.391 kb on - strand, within B158DRAFT_1821 at 1921.425 kb on + strand, within B158DRAFT_1821 at 1921.543 kb on + strand, within B158DRAFT_1821 at 1921.551 kb on - strand, within B158DRAFT_1821 at 1921.551 kb on - strand, within B158DRAFT_1821 at 1921.551 kb on - strand, within B158DRAFT_1821 at 1921.551 kb on - strand, within B158DRAFT_1821 at 1921.615 kb on - strand, within B158DRAFT_1821 at 1921.635 kb on + strand, within B158DRAFT_1821 at 1921.640 kb on + strand, within B158DRAFT_1821 at 1921.643 kb on - strand, within B158DRAFT_1821 at 1921.643 kb on - strand, within B158DRAFT_1821 at 1921.646 kb on - strand, within B158DRAFT_1821 at 1921.646 kb on - strand, within B158DRAFT_1821 at 1921.648 kb on - strand, within B158DRAFT_1821 at 1921.648 kb on - strand, within B158DRAFT_1821 at 1921.653 kb on - strand, within B158DRAFT_1821 at 1921.653 kb on - strand, within B158DRAFT_1821 at 1921.653 kb on - strand, within B158DRAFT_1821 at 1921.661 kb on - strand, within B158DRAFT_1821 at 1921.666 kb on - strand, within B158DRAFT_1821 at 1921.700 kb on - strand, within B158DRAFT_1821 at 1921.978 kb on + strand at 1921.978 kb on + strand at 1921.986 kb on - strand at 1921.986 kb on - strand at 1921.986 kb on - strand at 1921.991 kb on - strand at 1921.991 kb on - strand at 1921.991 kb on - strand at 1921.991 kb on - strand at 1922.096 kb on + strand at 1922.096 kb on + strand at 1922.096 kb on + strand at 1922.104 kb on - strand at 1922.120 kb on + strand at 1922.177 kb on + strand at 1922.213 kb on + strand, within B158DRAFT_1822 at 1922.221 kb on - strand, within B158DRAFT_1822 at 1922.221 kb on - strand, within B158DRAFT_1822 at 1922.334 kb on - strand, within B158DRAFT_1822 at 1922.419 kb on + strand, within B158DRAFT_1822 at 1922.427 kb on - strand, within B158DRAFT_1822 at 1922.451 kb on + strand, within B158DRAFT_1822 at 1922.451 kb on + strand, within B158DRAFT_1822 at 1922.456 kb on + strand, within B158DRAFT_1822 at 1922.459 kb on - strand, within B158DRAFT_1822 at 1922.489 kb on + strand, within B158DRAFT_1822 at 1922.497 kb on - strand, within B158DRAFT_1822 at 1922.499 kb on + strand, within B158DRAFT_1822 at 1922.499 kb on + strand, within B158DRAFT_1822 at 1922.499 kb on + strand, within B158DRAFT_1822 at 1922.499 kb on + strand, within B158DRAFT_1822 at 1922.499 kb on + strand, within B158DRAFT_1822 at 1922.499 kb on + strand, within B158DRAFT_1822 at 1922.499 kb on + strand, within B158DRAFT_1822 at 1922.499 kb on + strand, within B158DRAFT_1822 at 1922.499 kb on + strand, within B158DRAFT_1822 at 1922.499 kb on + strand, within B158DRAFT_1822 at 1922.499 kb on + strand, within B158DRAFT_1822 at 1922.499 kb on + strand, within B158DRAFT_1822 at 1922.499 kb on + strand, within B158DRAFT_1822 at 1922.507 kb on - strand, within B158DRAFT_1822 at 1922.507 kb on - strand, within B158DRAFT_1822 at 1922.507 kb on - strand, within B158DRAFT_1822 at 1922.507 kb on - strand, within B158DRAFT_1822 at 1922.507 kb on - strand, within B158DRAFT_1822 at 1922.507 kb on - strand, within B158DRAFT_1822 at 1922.507 kb on - strand, within B158DRAFT_1822 at 1922.507 kb on - strand, within B158DRAFT_1822 at 1922.507 kb on - strand, within B158DRAFT_1822 at 1922.507 kb on - strand, within B158DRAFT_1822 at 1922.507 kb on - strand, within B158DRAFT_1822 at 1922.511 kb on + strand, within B158DRAFT_1822 at 1922.511 kb on + strand, within B158DRAFT_1822 at 1922.512 kb on - strand, within B158DRAFT_1822 at 1922.519 kb on - strand, within B158DRAFT_1822 at 1922.587 kb on + strand, within B158DRAFT_1822 at 1922.592 kb on + strand at 1922.592 kb on + strand at 1922.592 kb on + strand at 1922.600 kb on - strand at 1922.600 kb on - strand at 1922.600 kb on - strand at 1922.600 kb on - strand at 1922.600 kb on - strand at 1922.600 kb on - strand at 1922.792 kb on - strand at 1922.837 kb on + strand at 1922.860 kb on + strand at 1923.029 kb on - strand, within B158DRAFT_1823 at 1923.040 kb on + strand, within B158DRAFT_1823 at 1923.040 kb on + strand, within B158DRAFT_1823
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Nickel (II) chloride 1.25 mM remove 1,919,189 + +0.4 1,919,189 + -1.1 1,919,189 + +0.4 1,919,189 + -0.9 1,919,197 - +0.9 1,919,197 - -0.6 1,919,207 - +0.3 1,919,237 + -1.6 1,919,263 + -2.5 1,919,263 + -2.9 1,919,271 - -0.2 1,919,271 - -0.2 1,919,403 + -1.2 1,919,419 + -2.5 1,919,440 + -1.3 1,919,448 - -0.6 1,919,474 + -2.2 1,919,482 - -0.3 1,919,646 + -1.2 1,919,719 - -3.0 1,919,738 - -1.9 1,919,738 - -1.7 1,919,748 + -1.1 1,919,752 - -1.0 1,919,754 - -1.1 1,919,795 + -2.1 1,919,813 + -1.0 1,919,859 + -1.8 1,919,867 - -2.6 1,919,915 + -1.8 1,919,923 - -1.4 1,919,923 - -0.4 1,919,953 - B158DRAFT_1821 0.10 -1.6 1,919,980 - B158DRAFT_1821 0.12 -1.2 1,920,077 - B158DRAFT_1821 0.16 -2.0 1,920,080 + B158DRAFT_1821 0.16 -2.3 1,920,083 - B158DRAFT_1821 0.16 -2.8 1,920,096 + B158DRAFT_1821 0.17 -1.5 1,920,098 + B158DRAFT_1821 0.17 -3.0 1,920,106 - B158DRAFT_1821 0.17 -1.6 1,920,106 - B158DRAFT_1821 0.17 -2.0 1,920,135 - B158DRAFT_1821 0.18 -0.6 1,920,142 + B158DRAFT_1821 0.19 -2.6 1,920,153 + B158DRAFT_1821 0.19 -3.5 1,920,212 + B158DRAFT_1821 0.22 -3.6 1,920,220 - B158DRAFT_1821 0.22 -0.8 1,920,360 - B158DRAFT_1821 0.28 -1.3 1,920,390 + B158DRAFT_1821 0.29 -2.8 1,920,549 + B158DRAFT_1821 0.36 -1.0 1,920,549 + B158DRAFT_1821 0.36 -2.3 1,920,549 + B158DRAFT_1821 0.36 -2.2 1,920,557 - B158DRAFT_1821 0.36 -2.1 1,920,562 - B158DRAFT_1821 0.36 -1.0 1,920,592 - B158DRAFT_1821 0.38 +0.3 1,920,625 - B158DRAFT_1821 0.39 -2.2 1,920,640 + B158DRAFT_1821 0.40 -1.6 1,920,689 - B158DRAFT_1821 0.42 -2.0 1,920,717 + B158DRAFT_1821 0.43 -2.4 1,920,738 + B158DRAFT_1821 0.44 -2.1 1,920,740 + B158DRAFT_1821 0.44 -2.3 1,920,803 + B158DRAFT_1821 0.47 -1.9 1,920,827 - B158DRAFT_1821 0.48 -1.4 1,920,839 - B158DRAFT_1821 0.48 -1.7 1,920,857 + B158DRAFT_1821 0.49 -2.7 1,920,857 + B158DRAFT_1821 0.49 -3.8 1,920,857 + B158DRAFT_1821 0.49 -1.5 1,920,857 + B158DRAFT_1821 0.49 -2.0 1,920,865 - B158DRAFT_1821 0.49 -3.0 1,920,865 - B158DRAFT_1821 0.49 -0.9 1,920,865 - B158DRAFT_1821 0.49 -0.4 1,920,865 - B158DRAFT_1821 0.49 -2.9 1,920,865 - B158DRAFT_1821 0.49 -4.3 1,920,865 - B158DRAFT_1821 0.49 -0.4 1,920,935 + B158DRAFT_1821 0.52 -2.7 1,921,073 + B158DRAFT_1821 0.58 -3.9 1,921,076 - B158DRAFT_1821 0.58 -0.1 1,921,076 - B158DRAFT_1821 0.58 -0.9 1,921,117 + B158DRAFT_1821 0.60 -3.4 1,921,173 - B158DRAFT_1821 0.63 -2.3 1,921,251 + B158DRAFT_1821 0.66 -3.0 1,921,259 - B158DRAFT_1821 0.66 -1.0 1,921,259 - B158DRAFT_1821 0.66 -1.5 1,921,263 + B158DRAFT_1821 0.66 -4.0 1,921,263 + B158DRAFT_1821 0.66 -0.7 1,921,263 + B158DRAFT_1821 0.66 -1.4 1,921,271 - B158DRAFT_1821 0.67 -0.8 1,921,271 - B158DRAFT_1821 0.67 -0.8 1,921,271 - B158DRAFT_1821 0.67 -1.4 1,921,279 + B158DRAFT_1821 0.67 -2.7 1,921,293 - B158DRAFT_1821 0.68 -1.6 1,921,298 - B158DRAFT_1821 0.68 -2.1 1,921,322 - B158DRAFT_1821 0.69 -0.6 1,921,333 - B158DRAFT_1821 0.69 -1.9 1,921,340 + B158DRAFT_1821 0.70 -1.6 1,921,348 - B158DRAFT_1821 0.70 +0.2 1,921,391 - B158DRAFT_1821 0.72 -3.4 1,921,391 - B158DRAFT_1821 0.72 -2.8 1,921,425 + B158DRAFT_1821 0.73 -2.6 1,921,543 + B158DRAFT_1821 0.78 -1.6 1,921,551 - B158DRAFT_1821 0.79 +0.6 1,921,551 - B158DRAFT_1821 0.79 -1.0 1,921,551 - B158DRAFT_1821 0.79 -0.6 1,921,551 - B158DRAFT_1821 0.79 -1.3 1,921,615 - B158DRAFT_1821 0.81 -0.4 1,921,635 + B158DRAFT_1821 0.82 -0.8 1,921,640 + B158DRAFT_1821 0.83 +0.0 1,921,643 - B158DRAFT_1821 0.83 -1.4 1,921,643 - B158DRAFT_1821 0.83 -1.6 1,921,646 - B158DRAFT_1821 0.83 -2.6 1,921,646 - B158DRAFT_1821 0.83 -1.3 1,921,648 - B158DRAFT_1821 0.83 +0.6 1,921,648 - B158DRAFT_1821 0.83 +0.6 1,921,653 - B158DRAFT_1821 0.83 -2.4 1,921,653 - B158DRAFT_1821 0.83 -1.9 1,921,653 - B158DRAFT_1821 0.83 -1.5 1,921,661 - B158DRAFT_1821 0.83 -1.7 1,921,666 - B158DRAFT_1821 0.84 -1.7 1,921,700 - B158DRAFT_1821 0.85 -1.3 1,921,978 + -2.1 1,921,978 + -2.4 1,921,986 - -2.4 1,921,986 - -1.9 1,921,986 - -3.0 1,921,991 - -1.1 1,921,991 - -1.7 1,921,991 - -1.1 1,921,991 - -1.7 1,922,096 + -0.4 1,922,096 + -0.7 1,922,096 + -0.3 1,922,104 - -0.0 1,922,120 + +0.4 1,922,177 + -0.5 1,922,213 + B158DRAFT_1822 0.15 -0.6 1,922,221 - B158DRAFT_1822 0.16 -0.5 1,922,221 - B158DRAFT_1822 0.16 -1.7 1,922,334 - B158DRAFT_1822 0.39 +0.3 1,922,419 + B158DRAFT_1822 0.56 -1.0 1,922,427 - B158DRAFT_1822 0.58 -0.8 1,922,451 + B158DRAFT_1822 0.62 -0.5 1,922,451 + B158DRAFT_1822 0.62 -0.7 1,922,456 + B158DRAFT_1822 0.63 -0.2 1,922,459 - B158DRAFT_1822 0.64 -0.4 1,922,489 + B158DRAFT_1822 0.70 -0.3 1,922,497 - B158DRAFT_1822 0.72 -0.6 1,922,499 + B158DRAFT_1822 0.72 -0.8 1,922,499 + B158DRAFT_1822 0.72 -0.8 1,922,499 + B158DRAFT_1822 0.72 -0.2 1,922,499 + B158DRAFT_1822 0.72 -0.1 1,922,499 + B158DRAFT_1822 0.72 -0.6 1,922,499 + B158DRAFT_1822 0.72 -1.4 1,922,499 + B158DRAFT_1822 0.72 -0.1 1,922,499 + B158DRAFT_1822 0.72 -1.3 1,922,499 + B158DRAFT_1822 0.72 -0.8 1,922,499 + B158DRAFT_1822 0.72 -1.2 1,922,499 + B158DRAFT_1822 0.72 -0.6 1,922,499 + B158DRAFT_1822 0.72 +0.1 1,922,499 + B158DRAFT_1822 0.72 -0.4 1,922,507 - B158DRAFT_1822 0.74 -0.5 1,922,507 - B158DRAFT_1822 0.74 -0.6 1,922,507 - B158DRAFT_1822 0.74 -0.3 1,922,507 - B158DRAFT_1822 0.74 -0.8 1,922,507 - B158DRAFT_1822 0.74 -0.9 1,922,507 - B158DRAFT_1822 0.74 -1.1 1,922,507 - B158DRAFT_1822 0.74 -0.5 1,922,507 - B158DRAFT_1822 0.74 -0.8 1,922,507 - B158DRAFT_1822 0.74 -0.3 1,922,507 - B158DRAFT_1822 0.74 -0.2 1,922,507 - B158DRAFT_1822 0.74 -1.1 1,922,511 + B158DRAFT_1822 0.74 -0.7 1,922,511 + B158DRAFT_1822 0.74 -0.5 1,922,512 - B158DRAFT_1822 0.75 -0.4 1,922,519 - B158DRAFT_1822 0.76 -0.3 1,922,587 + B158DRAFT_1822 0.90 +0.0 1,922,592 + -1.1 1,922,592 + -0.5 1,922,592 + -0.9 1,922,600 - -1.2 1,922,600 - -0.4 1,922,600 - -0.9 1,922,600 - -0.2 1,922,600 - +0.0 1,922,600 - -0.7 1,922,792 - +0.6 1,922,837 + -0.5 1,922,860 + -0.6 1,923,029 - B158DRAFT_1823 0.22 -0.7 1,923,040 + B158DRAFT_1823 0.23 -0.3 1,923,040 + B158DRAFT_1823 0.23 -1.8
Or see this region's nucleotide sequence