Experiment: m.b. Nickel (II) chloride 1.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_1581 and B158DRAFT_1582 are separated by 108 nucleotides B158DRAFT_1582 and B158DRAFT_1583 are separated by 87 nucleotides
B158DRAFT_1581: B158DRAFT_1581 - diguanylate cyclase (GGDEF) domain, at 1,640,710 to 1,642,599
_1581
B158DRAFT_1582: B158DRAFT_1582 - channel protein, hemolysin III family, at 1,642,708 to 1,643,403
_1582
B158DRAFT_1583: B158DRAFT_1583 - diguanylate cyclase, at 1,643,491 to 1,644,963
_1583
Position (kb)
1642
1643
1644 Strain fitness (log2 ratio)
-2
-1
0
1 at 1641.718 kb on - strand, within B158DRAFT_1581 at 1641.722 kb on + strand, within B158DRAFT_1581 at 1641.723 kb on + strand, within B158DRAFT_1581 at 1641.760 kb on + strand, within B158DRAFT_1581 at 1641.760 kb on + strand, within B158DRAFT_1581 at 1641.768 kb on - strand, within B158DRAFT_1581 at 1641.768 kb on - strand, within B158DRAFT_1581 at 1641.800 kb on - strand, within B158DRAFT_1581 at 1641.808 kb on - strand, within B158DRAFT_1581 at 1641.856 kb on + strand, within B158DRAFT_1581 at 1641.863 kb on + strand, within B158DRAFT_1581 at 1641.864 kb on - strand, within B158DRAFT_1581 at 1641.864 kb on - strand, within B158DRAFT_1581 at 1641.869 kb on - strand, within B158DRAFT_1581 at 1641.930 kb on - strand, within B158DRAFT_1581 at 1641.938 kb on - strand, within B158DRAFT_1581 at 1641.946 kb on - strand, within B158DRAFT_1581 at 1641.946 kb on - strand, within B158DRAFT_1581 at 1641.981 kb on + strand, within B158DRAFT_1581 at 1641.983 kb on + strand, within B158DRAFT_1581 at 1641.983 kb on + strand, within B158DRAFT_1581 at 1641.991 kb on - strand, within B158DRAFT_1581 at 1642.026 kb on - strand, within B158DRAFT_1581 at 1642.064 kb on - strand, within B158DRAFT_1581 at 1642.097 kb on + strand, within B158DRAFT_1581 at 1642.141 kb on - strand, within B158DRAFT_1581 at 1642.227 kb on + strand, within B158DRAFT_1581 at 1642.277 kb on - strand, within B158DRAFT_1581 at 1642.308 kb on + strand, within B158DRAFT_1581 at 1642.477 kb on + strand at 1642.478 kb on + strand at 1642.478 kb on + strand at 1642.479 kb on - strand at 1642.486 kb on - strand at 1642.496 kb on + strand at 1642.496 kb on + strand at 1642.504 kb on + strand at 1642.504 kb on - strand at 1642.504 kb on - strand at 1642.506 kb on + strand at 1642.511 kb on + strand at 1642.511 kb on + strand at 1642.511 kb on + strand at 1642.521 kb on + strand at 1642.546 kb on - strand at 1642.548 kb on + strand at 1642.567 kb on + strand at 1642.588 kb on + strand at 1642.606 kb on - strand at 1642.606 kb on - strand at 1642.641 kb on + strand at 1642.654 kb on + strand at 1642.683 kb on + strand at 1642.696 kb on - strand at 1642.696 kb on - strand at 1642.727 kb on + strand at 1642.727 kb on + strand at 1642.727 kb on + strand at 1642.727 kb on + strand at 1642.735 kb on - strand at 1642.737 kb on + strand at 1642.737 kb on + strand at 1642.807 kb on + strand, within B158DRAFT_1582 at 1642.807 kb on + strand, within B158DRAFT_1582 at 1642.807 kb on + strand, within B158DRAFT_1582 at 1642.814 kb on - strand, within B158DRAFT_1582 at 1642.815 kb on - strand, within B158DRAFT_1582 at 1642.815 kb on - strand, within B158DRAFT_1582 at 1642.815 kb on - strand, within B158DRAFT_1582 at 1642.820 kb on + strand, within B158DRAFT_1582 at 1642.826 kb on + strand, within B158DRAFT_1582 at 1642.838 kb on + strand, within B158DRAFT_1582 at 1642.846 kb on - strand, within B158DRAFT_1582 at 1642.860 kb on - strand, within B158DRAFT_1582 at 1642.863 kb on + strand, within B158DRAFT_1582 at 1642.863 kb on + strand, within B158DRAFT_1582 at 1642.871 kb on - strand, within B158DRAFT_1582 at 1642.871 kb on - strand, within B158DRAFT_1582 at 1642.912 kb on - strand, within B158DRAFT_1582 at 1642.973 kb on - strand, within B158DRAFT_1582 at 1643.000 kb on + strand, within B158DRAFT_1582 at 1643.000 kb on + strand, within B158DRAFT_1582 at 1643.000 kb on + strand, within B158DRAFT_1582 at 1643.000 kb on + strand, within B158DRAFT_1582 at 1643.008 kb on - strand, within B158DRAFT_1582 at 1643.008 kb on - strand, within B158DRAFT_1582 at 1643.047 kb on - strand, within B158DRAFT_1582 at 1643.063 kb on + strand, within B158DRAFT_1582 at 1643.072 kb on - strand, within B158DRAFT_1582 at 1643.074 kb on + strand, within B158DRAFT_1582 at 1643.074 kb on + strand, within B158DRAFT_1582 at 1643.082 kb on - strand, within B158DRAFT_1582 at 1643.177 kb on + strand, within B158DRAFT_1582 at 1643.177 kb on + strand, within B158DRAFT_1582 at 1643.185 kb on - strand, within B158DRAFT_1582 at 1643.196 kb on + strand, within B158DRAFT_1582 at 1643.240 kb on - strand, within B158DRAFT_1582 at 1643.274 kb on + strand, within B158DRAFT_1582 at 1643.277 kb on - strand, within B158DRAFT_1582 at 1643.277 kb on - strand, within B158DRAFT_1582 at 1643.282 kb on - strand, within B158DRAFT_1582 at 1643.282 kb on - strand, within B158DRAFT_1582 at 1643.335 kb on - strand at 1643.353 kb on - strand at 1643.365 kb on + strand at 1643.373 kb on + strand at 1643.445 kb on + strand at 1643.445 kb on + strand at 1643.453 kb on - strand at 1643.453 kb on - strand at 1643.481 kb on - strand at 1643.486 kb on + strand at 1643.501 kb on + strand at 1643.509 kb on - strand at 1643.569 kb on - strand at 1643.569 kb on - strand at 1643.569 kb on - strand at 1643.602 kb on - strand at 1643.616 kb on - strand at 1643.670 kb on + strand, within B158DRAFT_1583 at 1643.670 kb on + strand, within B158DRAFT_1583 at 1643.670 kb on + strand, within B158DRAFT_1583 at 1643.670 kb on + strand, within B158DRAFT_1583 at 1643.678 kb on - strand, within B158DRAFT_1583 at 1643.678 kb on - strand, within B158DRAFT_1583 at 1643.678 kb on - strand, within B158DRAFT_1583 at 1643.678 kb on - strand, within B158DRAFT_1583 at 1643.678 kb on - strand, within B158DRAFT_1583 at 1643.678 kb on - strand, within B158DRAFT_1583 at 1643.749 kb on + strand, within B158DRAFT_1583 at 1643.779 kb on - strand, within B158DRAFT_1583 at 1643.781 kb on + strand, within B158DRAFT_1583 at 1643.783 kb on + strand, within B158DRAFT_1583 at 1643.783 kb on + strand, within B158DRAFT_1583 at 1643.785 kb on - strand, within B158DRAFT_1583 at 1643.800 kb on + strand, within B158DRAFT_1583 at 1643.802 kb on + strand, within B158DRAFT_1583 at 1643.802 kb on + strand, within B158DRAFT_1583 at 1643.808 kb on - strand, within B158DRAFT_1583 at 1643.810 kb on - strand, within B158DRAFT_1583 at 1643.843 kb on + strand, within B158DRAFT_1583 at 1643.893 kb on - strand, within B158DRAFT_1583 at 1643.895 kb on + strand, within B158DRAFT_1583 at 1643.895 kb on + strand, within B158DRAFT_1583 at 1643.895 kb on - strand, within B158DRAFT_1583 at 1643.903 kb on - strand, within B158DRAFT_1583 at 1643.933 kb on - strand, within B158DRAFT_1583 at 1643.938 kb on - strand, within B158DRAFT_1583 at 1643.979 kb on + strand, within B158DRAFT_1583 at 1644.007 kb on + strand, within B158DRAFT_1583 at 1644.024 kb on - strand, within B158DRAFT_1583 at 1644.033 kb on - strand, within B158DRAFT_1583 at 1644.069 kb on + strand, within B158DRAFT_1583 at 1644.070 kb on + strand, within B158DRAFT_1583 at 1644.078 kb on - strand, within B158DRAFT_1583 at 1644.080 kb on - strand, within B158DRAFT_1583 at 1644.080 kb on - strand, within B158DRAFT_1583 at 1644.088 kb on - strand, within B158DRAFT_1583 at 1644.119 kb on - strand, within B158DRAFT_1583 at 1644.130 kb on - strand, within B158DRAFT_1583 at 1644.194 kb on + strand, within B158DRAFT_1583 at 1644.225 kb on - strand, within B158DRAFT_1583 at 1644.237 kb on - strand, within B158DRAFT_1583 at 1644.255 kb on + strand, within B158DRAFT_1583 at 1644.262 kb on + strand, within B158DRAFT_1583 at 1644.262 kb on + strand, within B158DRAFT_1583 at 1644.268 kb on + strand, within B158DRAFT_1583 at 1644.270 kb on - strand, within B158DRAFT_1583 at 1644.270 kb on - strand, within B158DRAFT_1583 at 1644.270 kb on - strand, within B158DRAFT_1583 at 1644.270 kb on - strand, within B158DRAFT_1583 at 1644.295 kb on - strand, within B158DRAFT_1583
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Nickel (II) chloride 1.25 mM remove 1,641,718 - B158DRAFT_1581 0.53 +0.4 1,641,722 + B158DRAFT_1581 0.54 +1.0 1,641,723 + B158DRAFT_1581 0.54 +0.1 1,641,760 + B158DRAFT_1581 0.56 +0.1 1,641,760 + B158DRAFT_1581 0.56 -1.2 1,641,768 - B158DRAFT_1581 0.56 -0.2 1,641,768 - B158DRAFT_1581 0.56 -0.5 1,641,800 - B158DRAFT_1581 0.58 +0.2 1,641,808 - B158DRAFT_1581 0.58 -0.2 1,641,856 + B158DRAFT_1581 0.61 -0.3 1,641,863 + B158DRAFT_1581 0.61 -0.1 1,641,864 - B158DRAFT_1581 0.61 -0.3 1,641,864 - B158DRAFT_1581 0.61 +0.2 1,641,869 - B158DRAFT_1581 0.61 -0.2 1,641,930 - B158DRAFT_1581 0.65 -1.0 1,641,938 - B158DRAFT_1581 0.65 +0.2 1,641,946 - B158DRAFT_1581 0.65 -0.1 1,641,946 - B158DRAFT_1581 0.65 +0.7 1,641,981 + B158DRAFT_1581 0.67 +0.0 1,641,983 + B158DRAFT_1581 0.67 -0.4 1,641,983 + B158DRAFT_1581 0.67 -0.2 1,641,991 - B158DRAFT_1581 0.68 -0.0 1,642,026 - B158DRAFT_1581 0.70 -1.2 1,642,064 - B158DRAFT_1581 0.72 -1.6 1,642,097 + B158DRAFT_1581 0.73 -1.5 1,642,141 - B158DRAFT_1581 0.76 +0.3 1,642,227 + B158DRAFT_1581 0.80 -0.0 1,642,277 - B158DRAFT_1581 0.83 -0.3 1,642,308 + B158DRAFT_1581 0.85 -0.7 1,642,477 + +0.0 1,642,478 + -0.4 1,642,478 + +0.5 1,642,479 - -0.4 1,642,486 - +0.1 1,642,496 + +0.1 1,642,496 + +0.3 1,642,504 + +1.6 1,642,504 - -0.1 1,642,504 - +0.1 1,642,506 + +0.2 1,642,511 + -0.4 1,642,511 + -1.9 1,642,511 + -0.2 1,642,521 + +0.0 1,642,546 - -0.1 1,642,548 + +1.2 1,642,567 + -1.1 1,642,588 + -0.1 1,642,606 - +0.1 1,642,606 - -0.4 1,642,641 + -0.2 1,642,654 + -1.0 1,642,683 + -0.8 1,642,696 - +0.3 1,642,696 - -0.2 1,642,727 + +0.5 1,642,727 + -0.0 1,642,727 + -0.8 1,642,727 + -0.1 1,642,735 - +0.2 1,642,737 + -0.3 1,642,737 + +0.1 1,642,807 + B158DRAFT_1582 0.14 -0.7 1,642,807 + B158DRAFT_1582 0.14 -0.6 1,642,807 + B158DRAFT_1582 0.14 -0.3 1,642,814 - B158DRAFT_1582 0.15 -0.2 1,642,815 - B158DRAFT_1582 0.15 -0.7 1,642,815 - B158DRAFT_1582 0.15 -0.8 1,642,815 - B158DRAFT_1582 0.15 +0.4 1,642,820 + B158DRAFT_1582 0.16 -0.7 1,642,826 + B158DRAFT_1582 0.17 -0.1 1,642,838 + B158DRAFT_1582 0.19 -0.1 1,642,846 - B158DRAFT_1582 0.20 -0.2 1,642,860 - B158DRAFT_1582 0.22 -0.6 1,642,863 + B158DRAFT_1582 0.22 -0.3 1,642,863 + B158DRAFT_1582 0.22 +0.0 1,642,871 - B158DRAFT_1582 0.23 -1.0 1,642,871 - B158DRAFT_1582 0.23 -0.1 1,642,912 - B158DRAFT_1582 0.29 -0.9 1,642,973 - B158DRAFT_1582 0.38 -0.2 1,643,000 + B158DRAFT_1582 0.42 -0.3 1,643,000 + B158DRAFT_1582 0.42 -0.2 1,643,000 + B158DRAFT_1582 0.42 -0.3 1,643,000 + B158DRAFT_1582 0.42 +0.1 1,643,008 - B158DRAFT_1582 0.43 -0.9 1,643,008 - B158DRAFT_1582 0.43 -0.5 1,643,047 - B158DRAFT_1582 0.49 -0.8 1,643,063 + B158DRAFT_1582 0.51 -0.4 1,643,072 - B158DRAFT_1582 0.52 +0.4 1,643,074 + B158DRAFT_1582 0.53 +0.3 1,643,074 + B158DRAFT_1582 0.53 -0.5 1,643,082 - B158DRAFT_1582 0.54 +0.4 1,643,177 + B158DRAFT_1582 0.67 -0.7 1,643,177 + B158DRAFT_1582 0.67 -0.3 1,643,185 - B158DRAFT_1582 0.69 +0.2 1,643,196 + B158DRAFT_1582 0.70 +0.6 1,643,240 - B158DRAFT_1582 0.76 -0.1 1,643,274 + B158DRAFT_1582 0.81 -0.4 1,643,277 - B158DRAFT_1582 0.82 -0.0 1,643,277 - B158DRAFT_1582 0.82 +0.0 1,643,282 - B158DRAFT_1582 0.82 +0.1 1,643,282 - B158DRAFT_1582 0.82 -0.6 1,643,335 - +0.0 1,643,353 - -0.2 1,643,365 + +0.7 1,643,373 + +0.1 1,643,445 + -0.2 1,643,445 + +0.1 1,643,453 - -1.0 1,643,453 - -0.8 1,643,481 - +0.0 1,643,486 + +0.3 1,643,501 + -0.1 1,643,509 - -0.4 1,643,569 - +0.2 1,643,569 - +0.1 1,643,569 - -0.0 1,643,602 - -0.0 1,643,616 - -0.5 1,643,670 + B158DRAFT_1583 0.12 +0.1 1,643,670 + B158DRAFT_1583 0.12 -1.0 1,643,670 + B158DRAFT_1583 0.12 +0.4 1,643,670 + B158DRAFT_1583 0.12 -0.5 1,643,678 - B158DRAFT_1583 0.13 +0.1 1,643,678 - B158DRAFT_1583 0.13 -0.2 1,643,678 - B158DRAFT_1583 0.13 +0.3 1,643,678 - B158DRAFT_1583 0.13 +0.4 1,643,678 - B158DRAFT_1583 0.13 -0.6 1,643,678 - B158DRAFT_1583 0.13 -0.7 1,643,749 + B158DRAFT_1583 0.18 +0.4 1,643,779 - B158DRAFT_1583 0.20 +0.2 1,643,781 + B158DRAFT_1583 0.20 -0.1 1,643,783 + B158DRAFT_1583 0.20 +0.0 1,643,783 + B158DRAFT_1583 0.20 -0.3 1,643,785 - B158DRAFT_1583 0.20 -0.5 1,643,800 + B158DRAFT_1583 0.21 -0.3 1,643,802 + B158DRAFT_1583 0.21 -0.1 1,643,802 + B158DRAFT_1583 0.21 -0.2 1,643,808 - B158DRAFT_1583 0.22 -0.4 1,643,810 - B158DRAFT_1583 0.22 +0.1 1,643,843 + B158DRAFT_1583 0.24 -0.1 1,643,893 - B158DRAFT_1583 0.27 +0.3 1,643,895 + B158DRAFT_1583 0.27 -0.0 1,643,895 + B158DRAFT_1583 0.27 +0.0 1,643,895 - B158DRAFT_1583 0.27 +0.3 1,643,903 - B158DRAFT_1583 0.28 -0.8 1,643,933 - B158DRAFT_1583 0.30 -2.2 1,643,938 - B158DRAFT_1583 0.30 -0.5 1,643,979 + B158DRAFT_1583 0.33 -0.2 1,644,007 + B158DRAFT_1583 0.35 -0.3 1,644,024 - B158DRAFT_1583 0.36 -0.7 1,644,033 - B158DRAFT_1583 0.37 -0.6 1,644,069 + B158DRAFT_1583 0.39 -0.3 1,644,070 + B158DRAFT_1583 0.39 -0.2 1,644,078 - B158DRAFT_1583 0.40 -0.5 1,644,080 - B158DRAFT_1583 0.40 -0.5 1,644,080 - B158DRAFT_1583 0.40 -0.8 1,644,088 - B158DRAFT_1583 0.41 +0.1 1,644,119 - B158DRAFT_1583 0.43 -0.3 1,644,130 - B158DRAFT_1583 0.43 +0.1 1,644,194 + B158DRAFT_1583 0.48 -0.5 1,644,225 - B158DRAFT_1583 0.50 -0.4 1,644,237 - B158DRAFT_1583 0.51 -0.0 1,644,255 + B158DRAFT_1583 0.52 -1.5 1,644,262 + B158DRAFT_1583 0.52 +0.4 1,644,262 + B158DRAFT_1583 0.52 +0.1 1,644,268 + B158DRAFT_1583 0.53 -0.7 1,644,270 - B158DRAFT_1583 0.53 +0.2 1,644,270 - B158DRAFT_1583 0.53 -0.4 1,644,270 - B158DRAFT_1583 0.53 -0.3 1,644,270 - B158DRAFT_1583 0.53 -0.3 1,644,295 - B158DRAFT_1583 0.55 -0.7
Or see this region's nucleotide sequence