Strain Fitness in Kangiella aquimarina DSM 16071 around B158DRAFT_0300

Experiment: m.b. Polymyxin B sulfate 0.0019531 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntB158DRAFT_0298 and B158DRAFT_0299 are separated by 146 nucleotidesB158DRAFT_0299 and B158DRAFT_0300 are separated by 23 nucleotidesB158DRAFT_0300 and B158DRAFT_0301 are separated by 255 nucleotides B158DRAFT_0298: B158DRAFT_0298 - Cysteine synthase, at 301,300 to 302,352 _0298 B158DRAFT_0299: B158DRAFT_0299 - Predicted proline hydroxylase, at 302,499 to 303,155 _0299 B158DRAFT_0300: B158DRAFT_0300 - diguanylate cyclase (GGDEF) domain, at 303,179 to 305,236 _0300 B158DRAFT_0301: B158DRAFT_0301 - Hemolysins and related proteins containing CBS domains, at 305,492 to 306,670 _0301 Position (kb) 303 304 305 306Strain fitness (log2 ratio) -2 -1 0 1at 302.183 kb on - strand, within B158DRAFT_0298at 302.354 kb on + strandat 302.396 kb on - strandat 302.412 kb on - strandat 302.496 kb on + strandat 302.504 kb on - strandat 302.504 kb on - strandat 302.504 kb on - strandat 302.586 kb on + strand, within B158DRAFT_0299at 302.591 kb on + strand, within B158DRAFT_0299at 302.591 kb on + strand, within B158DRAFT_0299at 302.594 kb on - strand, within B158DRAFT_0299at 302.599 kb on - strand, within B158DRAFT_0299at 302.599 kb on - strand, within B158DRAFT_0299at 302.599 kb on - strand, within B158DRAFT_0299at 302.599 kb on - strand, within B158DRAFT_0299at 302.750 kb on - strand, within B158DRAFT_0299at 302.758 kb on - strand, within B158DRAFT_0299at 302.758 kb on - strand, within B158DRAFT_0299at 302.811 kb on + strand, within B158DRAFT_0299at 302.885 kb on + strand, within B158DRAFT_0299at 302.925 kb on - strand, within B158DRAFT_0299at 302.977 kb on + strand, within B158DRAFT_0299at 302.985 kb on - strand, within B158DRAFT_0299at 303.038 kb on + strand, within B158DRAFT_0299at 303.071 kb on + strand, within B158DRAFT_0299at 303.193 kb on - strandat 303.297 kb on - strandat 303.409 kb on - strand, within B158DRAFT_0300at 303.440 kb on - strand, within B158DRAFT_0300at 303.660 kb on + strand, within B158DRAFT_0300at 303.660 kb on + strand, within B158DRAFT_0300at 303.668 kb on - strand, within B158DRAFT_0300at 303.668 kb on - strand, within B158DRAFT_0300at 303.673 kb on - strand, within B158DRAFT_0300at 303.745 kb on + strand, within B158DRAFT_0300at 303.814 kb on + strand, within B158DRAFT_0300at 303.874 kb on + strand, within B158DRAFT_0300at 303.980 kb on - strand, within B158DRAFT_0300at 303.981 kb on + strand, within B158DRAFT_0300at 303.987 kb on + strand, within B158DRAFT_0300at 304.089 kb on + strand, within B158DRAFT_0300at 304.097 kb on - strand, within B158DRAFT_0300at 304.097 kb on - strand, within B158DRAFT_0300at 304.097 kb on - strand, within B158DRAFT_0300at 304.099 kb on + strand, within B158DRAFT_0300at 304.107 kb on - strand, within B158DRAFT_0300at 304.112 kb on - strand, within B158DRAFT_0300at 304.145 kb on + strand, within B158DRAFT_0300at 304.226 kb on + strand, within B158DRAFT_0300at 304.229 kb on - strand, within B158DRAFT_0300at 304.239 kb on - strand, within B158DRAFT_0300at 304.319 kb on - strand, within B158DRAFT_0300at 304.323 kb on - strand, within B158DRAFT_0300at 304.485 kb on - strand, within B158DRAFT_0300at 304.536 kb on + strand, within B158DRAFT_0300at 304.551 kb on - strand, within B158DRAFT_0300at 304.600 kb on - strand, within B158DRAFT_0300at 304.600 kb on - strand, within B158DRAFT_0300at 304.600 kb on - strand, within B158DRAFT_0300at 304.600 kb on - strand, within B158DRAFT_0300at 304.600 kb on - strand, within B158DRAFT_0300at 304.611 kb on + strand, within B158DRAFT_0300at 304.631 kb on - strand, within B158DRAFT_0300at 304.715 kb on + strand, within B158DRAFT_0300at 304.732 kb on + strand, within B158DRAFT_0300at 304.752 kb on + strand, within B158DRAFT_0300at 304.814 kb on + strand, within B158DRAFT_0300at 304.814 kb on + strand, within B158DRAFT_0300at 304.814 kb on + strand, within B158DRAFT_0300at 304.822 kb on - strand, within B158DRAFT_0300at 304.822 kb on - strand, within B158DRAFT_0300at 304.822 kb on - strand, within B158DRAFT_0300at 304.822 kb on - strand, within B158DRAFT_0300at 304.842 kb on - strand, within B158DRAFT_0300at 304.925 kb on - strand, within B158DRAFT_0300at 304.925 kb on - strand, within B158DRAFT_0300at 304.925 kb on - strand, within B158DRAFT_0300at 304.925 kb on - strand, within B158DRAFT_0300at 304.975 kb on + strand, within B158DRAFT_0300at 304.975 kb on + strand, within B158DRAFT_0300at 305.069 kb on - strandat 305.096 kb on - strandat 305.133 kb on - strandat 305.257 kb on - strandat 305.321 kb on + strandat 305.329 kb on - strandat 305.549 kb on - strandat 305.549 kb on - strandat 305.585 kb on - strandat 305.585 kb on - strandat 305.585 kb on - strandat 305.587 kb on + strandat 305.595 kb on - strandat 305.643 kb on + strand, within B158DRAFT_0301at 305.650 kb on + strand, within B158DRAFT_0301at 305.650 kb on + strand, within B158DRAFT_0301at 305.650 kb on + strand, within B158DRAFT_0301at 305.650 kb on + strand, within B158DRAFT_0301at 305.690 kb on + strand, within B158DRAFT_0301at 305.740 kb on - strand, within B158DRAFT_0301at 305.774 kb on + strand, within B158DRAFT_0301at 305.781 kb on + strand, within B158DRAFT_0301at 305.781 kb on + strand, within B158DRAFT_0301at 305.789 kb on - strand, within B158DRAFT_0301at 305.789 kb on - strand, within B158DRAFT_0301at 305.789 kb on - strand, within B158DRAFT_0301at 305.920 kb on + strand, within B158DRAFT_0301at 305.920 kb on + strand, within B158DRAFT_0301at 305.928 kb on - strand, within B158DRAFT_0301at 305.928 kb on - strand, within B158DRAFT_0301at 305.928 kb on - strand, within B158DRAFT_0301at 305.944 kb on + strand, within B158DRAFT_0301at 305.944 kb on + strand, within B158DRAFT_0301at 305.944 kb on + strand, within B158DRAFT_0301at 305.944 kb on + strand, within B158DRAFT_0301at 305.944 kb on + strand, within B158DRAFT_0301at 305.944 kb on + strand, within B158DRAFT_0301at 305.944 kb on + strand, within B158DRAFT_0301at 305.944 kb on + strand, within B158DRAFT_0301at 305.944 kb on + strand, within B158DRAFT_0301at 305.952 kb on - strand, within B158DRAFT_0301at 305.952 kb on - strand, within B158DRAFT_0301at 305.952 kb on - strand, within B158DRAFT_0301at 305.952 kb on - strand, within B158DRAFT_0301at 305.952 kb on - strand, within B158DRAFT_0301at 305.952 kb on - strand, within B158DRAFT_0301at 306.012 kb on + strand, within B158DRAFT_0301at 306.020 kb on - strand, within B158DRAFT_0301at 306.036 kb on - strand, within B158DRAFT_0301at 306.176 kb on + strand, within B158DRAFT_0301at 306.186 kb on + strand, within B158DRAFT_0301at 306.205 kb on + strand, within B158DRAFT_0301at 306.213 kb on - strand, within B158DRAFT_0301at 306.213 kb on - strand, within B158DRAFT_0301

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Per-strain Table

Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0019531 mg/ml
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302,183 - B158DRAFT_0298 0.84 +0.3
302,354 + -0.3
302,396 - -0.6
302,412 - -0.1
302,496 + -0.0
302,504 - -0.4
302,504 - -0.3
302,504 - +0.0
302,586 + B158DRAFT_0299 0.13 -0.5
302,591 + B158DRAFT_0299 0.14 -2.2
302,591 + B158DRAFT_0299 0.14 -0.1
302,594 - B158DRAFT_0299 0.14 -0.5
302,599 - B158DRAFT_0299 0.15 +0.0
302,599 - B158DRAFT_0299 0.15 -0.2
302,599 - B158DRAFT_0299 0.15 +0.5
302,599 - B158DRAFT_0299 0.15 -0.5
302,750 - B158DRAFT_0299 0.38 -0.0
302,758 - B158DRAFT_0299 0.39 -0.2
302,758 - B158DRAFT_0299 0.39 +0.6
302,811 + B158DRAFT_0299 0.47 +0.0
302,885 + B158DRAFT_0299 0.59 -0.8
302,925 - B158DRAFT_0299 0.65 -0.8
302,977 + B158DRAFT_0299 0.73 -1.1
302,985 - B158DRAFT_0299 0.74 +0.4
303,038 + B158DRAFT_0299 0.82 -0.4
303,071 + B158DRAFT_0299 0.87 +0.1
303,193 - -0.2
303,297 - -0.4
303,409 - B158DRAFT_0300 0.11 -0.5
303,440 - B158DRAFT_0300 0.13 -0.6
303,660 + B158DRAFT_0300 0.23 +0.4
303,660 + B158DRAFT_0300 0.23 -1.0
303,668 - B158DRAFT_0300 0.24 -0.8
303,668 - B158DRAFT_0300 0.24 -0.3
303,673 - B158DRAFT_0300 0.24 -0.5
303,745 + B158DRAFT_0300 0.28 -1.5
303,814 + B158DRAFT_0300 0.31 -0.1
303,874 + B158DRAFT_0300 0.34 -0.5
303,980 - B158DRAFT_0300 0.39 -2.4
303,981 + B158DRAFT_0300 0.39 -0.4
303,987 + B158DRAFT_0300 0.39 -0.5
304,089 + B158DRAFT_0300 0.44 -0.6
304,097 - B158DRAFT_0300 0.45 +0.7
304,097 - B158DRAFT_0300 0.45 +0.2
304,097 - B158DRAFT_0300 0.45 -0.5
304,099 + B158DRAFT_0300 0.45 -0.1
304,107 - B158DRAFT_0300 0.45 +0.5
304,112 - B158DRAFT_0300 0.45 -0.6
304,145 + B158DRAFT_0300 0.47 +0.2
304,226 + B158DRAFT_0300 0.51 -0.2
304,229 - B158DRAFT_0300 0.51 -0.3
304,239 - B158DRAFT_0300 0.52 -0.8
304,319 - B158DRAFT_0300 0.55 -0.4
304,323 - B158DRAFT_0300 0.56 -0.3
304,485 - B158DRAFT_0300 0.63 -0.7
304,536 + B158DRAFT_0300 0.66 +0.3
304,551 - B158DRAFT_0300 0.67 -0.5
304,600 - B158DRAFT_0300 0.69 +0.4
304,600 - B158DRAFT_0300 0.69 -1.3
304,600 - B158DRAFT_0300 0.69 -0.2
304,600 - B158DRAFT_0300 0.69 -0.4
304,600 - B158DRAFT_0300 0.69 -0.2
304,611 + B158DRAFT_0300 0.70 -0.1
304,631 - B158DRAFT_0300 0.71 +0.1
304,715 + B158DRAFT_0300 0.75 -0.3
304,732 + B158DRAFT_0300 0.75 -0.0
304,752 + B158DRAFT_0300 0.76 -0.5
304,814 + B158DRAFT_0300 0.79 -1.0
304,814 + B158DRAFT_0300 0.79 -0.5
304,814 + B158DRAFT_0300 0.79 -1.4
304,822 - B158DRAFT_0300 0.80 -0.3
304,822 - B158DRAFT_0300 0.80 +0.0
304,822 - B158DRAFT_0300 0.80 -2.4
304,822 - B158DRAFT_0300 0.80 -0.3
304,842 - B158DRAFT_0300 0.81 -0.5
304,925 - B158DRAFT_0300 0.85 -0.7
304,925 - B158DRAFT_0300 0.85 -1.4
304,925 - B158DRAFT_0300 0.85 -0.0
304,925 - B158DRAFT_0300 0.85 -0.2
304,975 + B158DRAFT_0300 0.87 -0.3
304,975 + B158DRAFT_0300 0.87 -0.0
305,069 - -0.5
305,096 - -0.6
305,133 - -0.4
305,257 - -0.3
305,321 + +0.1
305,329 - -0.1
305,549 - +0.2
305,549 - -0.0
305,585 - -0.4
305,585 - -0.7
305,585 - -0.3
305,587 + +0.9
305,595 - -0.1
305,643 + B158DRAFT_0301 0.13 +0.3
305,650 + B158DRAFT_0301 0.13 -1.1
305,650 + B158DRAFT_0301 0.13 +0.7
305,650 + B158DRAFT_0301 0.13 +1.4
305,650 + B158DRAFT_0301 0.13 +0.5
305,690 + B158DRAFT_0301 0.17 -0.4
305,740 - B158DRAFT_0301 0.21 +0.3
305,774 + B158DRAFT_0301 0.24 +0.0
305,781 + B158DRAFT_0301 0.25 -0.0
305,781 + B158DRAFT_0301 0.25 -0.4
305,789 - B158DRAFT_0301 0.25 -0.0
305,789 - B158DRAFT_0301 0.25 +0.1
305,789 - B158DRAFT_0301 0.25 -1.6
305,920 + B158DRAFT_0301 0.36 -0.1
305,920 + B158DRAFT_0301 0.36 +0.2
305,928 - B158DRAFT_0301 0.37 -0.5
305,928 - B158DRAFT_0301 0.37 +1.0
305,928 - B158DRAFT_0301 0.37 +0.1
305,944 + B158DRAFT_0301 0.38 -0.3
305,944 + B158DRAFT_0301 0.38 -0.2
305,944 + B158DRAFT_0301 0.38 -0.3
305,944 + B158DRAFT_0301 0.38 +0.7
305,944 + B158DRAFT_0301 0.38 +0.1
305,944 + B158DRAFT_0301 0.38 +0.0
305,944 + B158DRAFT_0301 0.38 -0.5
305,944 + B158DRAFT_0301 0.38 -0.2
305,944 + B158DRAFT_0301 0.38 +0.4
305,952 - B158DRAFT_0301 0.39 -0.5
305,952 - B158DRAFT_0301 0.39 -0.1
305,952 - B158DRAFT_0301 0.39 +0.1
305,952 - B158DRAFT_0301 0.39 -0.0
305,952 - B158DRAFT_0301 0.39 -0.4
305,952 - B158DRAFT_0301 0.39 -0.1
306,012 + B158DRAFT_0301 0.44 +0.2
306,020 - B158DRAFT_0301 0.45 -0.2
306,036 - B158DRAFT_0301 0.46 -0.0
306,176 + B158DRAFT_0301 0.58 -0.7
306,186 + B158DRAFT_0301 0.59 -1.4
306,205 + B158DRAFT_0301 0.60 -1.1
306,213 - B158DRAFT_0301 0.61 -0.2
306,213 - B158DRAFT_0301 0.61 -0.3

Or see this region's nucleotide sequence