Experiment: m.b. Polymyxin B sulfate 0.0019531 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_0255 and B158DRAFT_0256 are separated by 46 nucleotides B158DRAFT_0256 and B158DRAFT_0257 are separated by 288 nucleotides B158DRAFT_0257 and B158DRAFT_0258 are separated by 7 nucleotides
B158DRAFT_0255: B158DRAFT_0255 - Uncharacterized enzyme of heme biosynthesis, at 261,180 to 262,406
_0255
B158DRAFT_0256: B158DRAFT_0256 - hypothetical protein, at 262,453 to 262,686
_0256
B158DRAFT_0257: B158DRAFT_0257 - conserved hypothetical metal-binding protein, at 262,975 to 263,238
_0257
B158DRAFT_0258: B158DRAFT_0258 - transporter, NhaC family (TC 2.A.35), at 263,246 to 264,598
_0258
Position (kb)
262
263 Strain fitness (log2 ratio)
-2
-1
0
1 at 261.464 kb on + strand, within B158DRAFT_0255 at 261.464 kb on + strand, within B158DRAFT_0255 at 261.464 kb on + strand, within B158DRAFT_0255 at 261.464 kb on + strand, within B158DRAFT_0255 at 261.464 kb on + strand, within B158DRAFT_0255 at 261.472 kb on - strand, within B158DRAFT_0255 at 261.472 kb on - strand, within B158DRAFT_0255 at 261.472 kb on - strand, within B158DRAFT_0255 at 261.500 kb on + strand, within B158DRAFT_0255 at 261.500 kb on + strand, within B158DRAFT_0255 at 261.500 kb on + strand, within B158DRAFT_0255 at 261.500 kb on + strand, within B158DRAFT_0255 at 261.508 kb on - strand, within B158DRAFT_0255 at 261.508 kb on - strand, within B158DRAFT_0255 at 261.508 kb on - strand, within B158DRAFT_0255 at 261.508 kb on - strand, within B158DRAFT_0255 at 261.523 kb on + strand, within B158DRAFT_0255 at 261.533 kb on + strand, within B158DRAFT_0255 at 261.562 kb on + strand, within B158DRAFT_0255 at 261.570 kb on - strand, within B158DRAFT_0255 at 261.575 kb on - strand, within B158DRAFT_0255 at 261.579 kb on + strand, within B158DRAFT_0255 at 261.640 kb on + strand, within B158DRAFT_0255 at 261.645 kb on + strand, within B158DRAFT_0255 at 261.658 kb on - strand, within B158DRAFT_0255 at 261.714 kb on - strand, within B158DRAFT_0255 at 261.784 kb on + strand, within B158DRAFT_0255 at 261.854 kb on - strand, within B158DRAFT_0255 at 261.915 kb on + strand, within B158DRAFT_0255 at 261.928 kb on - strand, within B158DRAFT_0255 at 261.928 kb on - strand, within B158DRAFT_0255 at 261.928 kb on - strand, within B158DRAFT_0255 at 261.954 kb on - strand, within B158DRAFT_0255 at 261.954 kb on - strand, within B158DRAFT_0255 at 261.954 kb on - strand, within B158DRAFT_0255 at 261.970 kb on + strand, within B158DRAFT_0255 at 261.970 kb on + strand, within B158DRAFT_0255 at 261.973 kb on - strand, within B158DRAFT_0255 at 261.978 kb on - strand, within B158DRAFT_0255 at 261.978 kb on - strand, within B158DRAFT_0255 at 261.978 kb on - strand, within B158DRAFT_0255 at 261.978 kb on - strand, within B158DRAFT_0255 at 261.978 kb on - strand, within B158DRAFT_0255 at 262.006 kb on - strand, within B158DRAFT_0255 at 262.034 kb on + strand, within B158DRAFT_0255 at 262.042 kb on - strand, within B158DRAFT_0255 at 262.157 kb on - strand, within B158DRAFT_0255 at 262.167 kb on - strand, within B158DRAFT_0255 at 262.169 kb on + strand, within B158DRAFT_0255 at 262.177 kb on - strand, within B158DRAFT_0255 at 262.177 kb on - strand, within B158DRAFT_0255 at 262.177 kb on - strand, within B158DRAFT_0255 at 262.189 kb on - strand, within B158DRAFT_0255 at 262.269 kb on + strand, within B158DRAFT_0255 at 262.269 kb on - strand, within B158DRAFT_0255 at 262.271 kb on + strand, within B158DRAFT_0255 at 262.276 kb on + strand, within B158DRAFT_0255 at 262.279 kb on - strand, within B158DRAFT_0255 at 262.306 kb on + strand at 262.488 kb on + strand, within B158DRAFT_0256 at 262.488 kb on + strand, within B158DRAFT_0256 at 262.496 kb on - strand, within B158DRAFT_0256 at 262.496 kb on - strand, within B158DRAFT_0256 at 262.575 kb on - strand, within B158DRAFT_0256 at 262.620 kb on - strand, within B158DRAFT_0256 at 262.620 kb on - strand, within B158DRAFT_0256 at 262.628 kb on - strand, within B158DRAFT_0256 at 262.628 kb on - strand, within B158DRAFT_0256 at 262.690 kb on + strand at 262.734 kb on + strand at 262.749 kb on + strand at 262.821 kb on + strand at 262.929 kb on + strand at 262.929 kb on + strand at 262.929 kb on + strand at 262.931 kb on - strand at 262.933 kb on + strand at 262.937 kb on - strand at 262.937 kb on - strand at 262.937 kb on - strand at 262.942 kb on - strand at 262.950 kb on + strand at 262.981 kb on - strand at 263.132 kb on - strand, within B158DRAFT_0257 at 263.132 kb on - strand, within B158DRAFT_0257 at 263.251 kb on - strand at 263.272 kb on - strand at 263.272 kb on - strand at 263.304 kb on + strand at 263.345 kb on + strand at 263.351 kb on + strand at 263.374 kb on - strand at 263.376 kb on - strand at 263.539 kb on - strand, within B158DRAFT_0258 at 263.564 kb on + strand, within B158DRAFT_0258 at 263.581 kb on - strand, within B158DRAFT_0258
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0019531 mg/ml remove 261,464 + B158DRAFT_0255 0.23 +0.2 261,464 + B158DRAFT_0255 0.23 +0.3 261,464 + B158DRAFT_0255 0.23 -0.5 261,464 + B158DRAFT_0255 0.23 +0.1 261,464 + B158DRAFT_0255 0.23 +0.5 261,472 - B158DRAFT_0255 0.24 +0.0 261,472 - B158DRAFT_0255 0.24 +0.4 261,472 - B158DRAFT_0255 0.24 +0.2 261,500 + B158DRAFT_0255 0.26 +0.2 261,500 + B158DRAFT_0255 0.26 +0.7 261,500 + B158DRAFT_0255 0.26 +0.2 261,500 + B158DRAFT_0255 0.26 +0.3 261,508 - B158DRAFT_0255 0.27 +0.3 261,508 - B158DRAFT_0255 0.27 +0.3 261,508 - B158DRAFT_0255 0.27 +0.1 261,508 - B158DRAFT_0255 0.27 +0.5 261,523 + B158DRAFT_0255 0.28 +0.4 261,533 + B158DRAFT_0255 0.29 +0.4 261,562 + B158DRAFT_0255 0.31 -0.4 261,570 - B158DRAFT_0255 0.32 +0.2 261,575 - B158DRAFT_0255 0.32 -0.2 261,579 + B158DRAFT_0255 0.33 +0.7 261,640 + B158DRAFT_0255 0.37 +0.1 261,645 + B158DRAFT_0255 0.38 +0.7 261,658 - B158DRAFT_0255 0.39 -0.3 261,714 - B158DRAFT_0255 0.44 +0.3 261,784 + B158DRAFT_0255 0.49 +0.4 261,854 - B158DRAFT_0255 0.55 +0.4 261,915 + B158DRAFT_0255 0.60 +0.3 261,928 - B158DRAFT_0255 0.61 +0.4 261,928 - B158DRAFT_0255 0.61 -0.0 261,928 - B158DRAFT_0255 0.61 +0.7 261,954 - B158DRAFT_0255 0.63 -0.6 261,954 - B158DRAFT_0255 0.63 +0.0 261,954 - B158DRAFT_0255 0.63 +0.1 261,970 + B158DRAFT_0255 0.64 -0.4 261,970 + B158DRAFT_0255 0.64 +0.0 261,973 - B158DRAFT_0255 0.65 +0.5 261,978 - B158DRAFT_0255 0.65 +1.4 261,978 - B158DRAFT_0255 0.65 +0.0 261,978 - B158DRAFT_0255 0.65 -0.7 261,978 - B158DRAFT_0255 0.65 -0.8 261,978 - B158DRAFT_0255 0.65 -0.3 262,006 - B158DRAFT_0255 0.67 -0.1 262,034 + B158DRAFT_0255 0.70 +0.3 262,042 - B158DRAFT_0255 0.70 -1.3 262,157 - B158DRAFT_0255 0.80 +0.4 262,167 - B158DRAFT_0255 0.80 +0.1 262,169 + B158DRAFT_0255 0.81 -0.1 262,177 - B158DRAFT_0255 0.81 +0.9 262,177 - B158DRAFT_0255 0.81 +0.0 262,177 - B158DRAFT_0255 0.81 -0.5 262,189 - B158DRAFT_0255 0.82 -0.2 262,269 + B158DRAFT_0255 0.89 +0.0 262,269 - B158DRAFT_0255 0.89 +0.8 262,271 + B158DRAFT_0255 0.89 +1.4 262,276 + B158DRAFT_0255 0.89 -0.6 262,279 - B158DRAFT_0255 0.90 +0.3 262,306 + -0.7 262,488 + B158DRAFT_0256 0.15 +0.5 262,488 + B158DRAFT_0256 0.15 -0.2 262,496 - B158DRAFT_0256 0.18 -1.0 262,496 - B158DRAFT_0256 0.18 +0.3 262,575 - B158DRAFT_0256 0.52 +0.0 262,620 - B158DRAFT_0256 0.71 +0.1 262,620 - B158DRAFT_0256 0.71 -0.7 262,628 - B158DRAFT_0256 0.75 -0.0 262,628 - B158DRAFT_0256 0.75 -0.5 262,690 + -0.1 262,734 + -0.8 262,749 + +0.5 262,821 + -0.1 262,929 + -0.3 262,929 + -0.4 262,929 + +0.0 262,931 - +0.3 262,933 + -0.4 262,937 - +0.1 262,937 - -0.2 262,937 - -0.1 262,942 - -0.4 262,950 + -0.7 262,981 - -0.2 263,132 - B158DRAFT_0257 0.59 -0.2 263,132 - B158DRAFT_0257 0.59 +0.9 263,251 - -2.4 263,272 - -0.4 263,272 - -1.0 263,304 + -1.0 263,345 + -0.9 263,351 + -0.4 263,374 - -1.9 263,376 - -0.9 263,539 - B158DRAFT_0258 0.22 -1.7 263,564 + B158DRAFT_0258 0.24 -1.6 263,581 - B158DRAFT_0258 0.25 -1.4
Or see this region's nucleotide sequence