Experiment: m.b. Nickel (II) chloride 1.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_2464 and B158DRAFT_2465 are separated by 140 nucleotides B158DRAFT_2465 and B158DRAFT_2466 are separated by 72 nucleotides
B158DRAFT_2464: B158DRAFT_2464 - Outer membrane protein, at 643,604 to 644,959
_2464
B158DRAFT_2465: B158DRAFT_2465 - hypothetical protein, at 645,100 to 645,510
_2465
B158DRAFT_2466: B158DRAFT_2466 - methionine synthase (B12-dependent) (EC 2.1.1.13), at 645,583 to 649,266
_2466
Position (kb)
645
646 Strain fitness (log2 ratio)
-1
0
1 at 644.381 kb on - strand, within B158DRAFT_2464 at 644.419 kb on + strand, within B158DRAFT_2464 at 644.465 kb on - strand, within B158DRAFT_2464 at 644.476 kb on + strand, within B158DRAFT_2464 at 644.491 kb on - strand, within B158DRAFT_2464 at 644.491 kb on - strand, within B158DRAFT_2464 at 644.578 kb on - strand, within B158DRAFT_2464 at 644.625 kb on + strand, within B158DRAFT_2464 at 644.625 kb on + strand, within B158DRAFT_2464 at 644.625 kb on + strand, within B158DRAFT_2464 at 644.633 kb on - strand, within B158DRAFT_2464 at 644.633 kb on - strand, within B158DRAFT_2464 at 644.633 kb on - strand, within B158DRAFT_2464 at 644.643 kb on - strand, within B158DRAFT_2464 at 644.677 kb on + strand, within B158DRAFT_2464 at 644.735 kb on + strand, within B158DRAFT_2464 at 644.751 kb on - strand, within B158DRAFT_2464 at 644.762 kb on + strand, within B158DRAFT_2464 at 644.853 kb on - strand at 644.853 kb on - strand at 644.883 kb on - strand at 645.031 kb on - strand at 645.070 kb on + strand at 645.070 kb on + strand at 645.078 kb on - strand at 645.078 kb on - strand at 645.078 kb on - strand at 645.078 kb on - strand at 645.087 kb on + strand at 645.087 kb on + strand at 645.087 kb on + strand at 645.087 kb on + strand at 645.095 kb on - strand at 645.095 kb on - strand at 645.099 kb on + strand at 645.128 kb on + strand at 645.148 kb on + strand, within B158DRAFT_2465 at 645.209 kb on + strand, within B158DRAFT_2465 at 645.223 kb on + strand, within B158DRAFT_2465 at 645.231 kb on - strand, within B158DRAFT_2465 at 645.272 kb on - strand, within B158DRAFT_2465 at 645.324 kb on - strand, within B158DRAFT_2465 at 645.328 kb on + strand, within B158DRAFT_2465 at 645.328 kb on + strand, within B158DRAFT_2465 at 645.338 kb on + strand, within B158DRAFT_2465 at 645.343 kb on + strand, within B158DRAFT_2465 at 645.346 kb on - strand, within B158DRAFT_2465 at 645.350 kb on - strand, within B158DRAFT_2465 at 645.393 kb on + strand, within B158DRAFT_2465 at 645.393 kb on + strand, within B158DRAFT_2465 at 645.408 kb on + strand, within B158DRAFT_2465 at 645.457 kb on + strand, within B158DRAFT_2465 at 645.465 kb on - strand, within B158DRAFT_2465 at 645.470 kb on - strand at 645.490 kb on + strand at 645.506 kb on - strand at 645.539 kb on - strand at 645.600 kb on + strand at 645.603 kb on - strand at 645.606 kb on + strand at 645.608 kb on - strand at 645.608 kb on - strand at 645.608 kb on - strand at 645.608 kb on - strand at 645.608 kb on - strand at 645.626 kb on + strand at 645.634 kb on + strand at 645.634 kb on + strand at 645.641 kb on + strand at 645.641 kb on + strand at 645.649 kb on - strand at 645.649 kb on - strand at 645.649 kb on - strand at 645.659 kb on - strand at 645.659 kb on - strand at 645.688 kb on - strand at 645.727 kb on + strand at 645.740 kb on + strand at 645.743 kb on + strand at 645.767 kb on + strand at 645.779 kb on + strand at 645.813 kb on - strand at 645.813 kb on - strand at 645.815 kb on + strand at 645.823 kb on - strand at 645.825 kb on + strand at 645.849 kb on - strand at 645.850 kb on + strand at 645.850 kb on + strand at 645.850 kb on + strand at 645.850 kb on + strand at 645.858 kb on - strand at 645.858 kb on - strand at 645.858 kb on - strand at 645.858 kb on - strand at 645.860 kb on + strand at 645.860 kb on + strand at 645.896 kb on + strand at 645.970 kb on + strand, within B158DRAFT_2466 at 645.983 kb on + strand, within B158DRAFT_2466 at 646.034 kb on - strand, within B158DRAFT_2466 at 646.040 kb on + strand, within B158DRAFT_2466 at 646.085 kb on + strand, within B158DRAFT_2466 at 646.219 kb on - strand, within B158DRAFT_2466 at 646.223 kb on + strand, within B158DRAFT_2466 at 646.238 kb on - strand, within B158DRAFT_2466
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Nickel (II) chloride 1.25 mM remove 644,381 - B158DRAFT_2464 0.57 -0.5 644,419 + B158DRAFT_2464 0.60 -0.6 644,465 - B158DRAFT_2464 0.63 -0.1 644,476 + B158DRAFT_2464 0.64 +1.1 644,491 - B158DRAFT_2464 0.65 +0.4 644,491 - B158DRAFT_2464 0.65 +0.1 644,578 - B158DRAFT_2464 0.72 +0.7 644,625 + B158DRAFT_2464 0.75 +0.0 644,625 + B158DRAFT_2464 0.75 -0.2 644,625 + B158DRAFT_2464 0.75 +0.0 644,633 - B158DRAFT_2464 0.76 +0.0 644,633 - B158DRAFT_2464 0.76 -0.0 644,633 - B158DRAFT_2464 0.76 -0.2 644,643 - B158DRAFT_2464 0.77 -0.3 644,677 + B158DRAFT_2464 0.79 +0.7 644,735 + B158DRAFT_2464 0.83 +0.4 644,751 - B158DRAFT_2464 0.85 -0.4 644,762 + B158DRAFT_2464 0.85 +0.2 644,853 - +0.4 644,853 - -1.0 644,883 - -0.8 645,031 - +0.1 645,070 + +0.3 645,070 + -0.0 645,078 - -0.1 645,078 - -0.7 645,078 - +0.1 645,078 - -0.0 645,087 + +0.2 645,087 + -0.5 645,087 + +0.6 645,087 + -0.1 645,095 - -0.4 645,095 - +0.2 645,099 + -0.1 645,128 + +0.1 645,148 + B158DRAFT_2465 0.12 -0.6 645,209 + B158DRAFT_2465 0.27 -0.2 645,223 + B158DRAFT_2465 0.30 +0.1 645,231 - B158DRAFT_2465 0.32 -0.4 645,272 - B158DRAFT_2465 0.42 +0.4 645,324 - B158DRAFT_2465 0.55 -0.0 645,328 + B158DRAFT_2465 0.55 -0.6 645,328 + B158DRAFT_2465 0.55 -0.1 645,338 + B158DRAFT_2465 0.58 -0.1 645,343 + B158DRAFT_2465 0.59 +0.2 645,346 - B158DRAFT_2465 0.60 +0.1 645,350 - B158DRAFT_2465 0.61 -0.6 645,393 + B158DRAFT_2465 0.71 +1.2 645,393 + B158DRAFT_2465 0.71 -1.5 645,408 + B158DRAFT_2465 0.75 -0.7 645,457 + B158DRAFT_2465 0.87 +0.1 645,465 - B158DRAFT_2465 0.89 -0.7 645,470 - -0.5 645,490 + -0.1 645,506 - -0.4 645,539 - -0.3 645,600 + -0.5 645,603 - +0.4 645,606 + +0.0 645,608 - -0.6 645,608 - +0.1 645,608 - +1.2 645,608 - -0.6 645,608 - -0.2 645,626 + +0.1 645,634 + +0.4 645,634 + +0.4 645,641 + -0.2 645,641 + +0.1 645,649 - -0.5 645,649 - +0.1 645,649 - +0.4 645,659 - -0.7 645,659 - -0.0 645,688 - +0.2 645,727 + -0.8 645,740 + -0.1 645,743 + -0.9 645,767 + +0.1 645,779 + +0.1 645,813 - -0.0 645,813 - -0.0 645,815 + +0.4 645,823 - -0.4 645,825 + +0.1 645,849 - -1.3 645,850 + +0.1 645,850 + -0.6 645,850 + +1.4 645,850 + +0.1 645,858 - -0.3 645,858 - -0.4 645,858 - -0.5 645,858 - -1.1 645,860 + -0.0 645,860 + -0.2 645,896 + -0.1 645,970 + B158DRAFT_2466 0.11 -0.4 645,983 + B158DRAFT_2466 0.11 -0.8 646,034 - B158DRAFT_2466 0.12 -1.0 646,040 + B158DRAFT_2466 0.12 -0.5 646,085 + B158DRAFT_2466 0.14 +0.5 646,219 - B158DRAFT_2466 0.17 -0.1 646,223 + B158DRAFT_2466 0.17 -0.7 646,238 - B158DRAFT_2466 0.18 -0.4
Or see this region's nucleotide sequence