Experiment: m.b. Nickel (II) chloride 1.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_1490 and B158DRAFT_1491 are separated by 37 nucleotides B158DRAFT_1491 and B158DRAFT_1492 overlap by 1 nucleotides B158DRAFT_1492 and B158DRAFT_1493 are separated by 238 nucleotides B158DRAFT_1493 and B158DRAFT_1494 are separated by 220 nucleotides
B158DRAFT_1490: B158DRAFT_1490 - diguanylate cyclase (GGDEF) domain, at 1,544,677 to 1,548,054
_1490
B158DRAFT_1491: B158DRAFT_1491 - hypothetical protein, at 1,548,092 to 1,548,880
_1491
B158DRAFT_1492: B158DRAFT_1492 - FAD/FMN-containing dehydrogenases, at 1,548,880 to 1,550,271
_1492
B158DRAFT_1493: B158DRAFT_1493 - tRNA_Val_TAC, at 1,550,510 to 1,550,585
_1493
B158DRAFT_1494: B158DRAFT_1494 - glutamyl-tRNA synthetase (EC 6.1.1.17), at 1,550,806 to 1,552,224
_1494
Position (kb)
1548
1549
1550
1551 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1548.042 kb on + strand at 1548.085 kb on + strand at 1548.093 kb on - strand at 1548.095 kb on + strand at 1548.103 kb on - strand at 1548.118 kb on - strand at 1548.130 kb on - strand at 1548.181 kb on + strand, within B158DRAFT_1491 at 1548.208 kb on + strand, within B158DRAFT_1491 at 1548.234 kb on - strand, within B158DRAFT_1491 at 1548.234 kb on - strand, within B158DRAFT_1491 at 1548.244 kb on + strand, within B158DRAFT_1491 at 1548.252 kb on - strand, within B158DRAFT_1491 at 1548.257 kb on - strand, within B158DRAFT_1491 at 1548.288 kb on - strand, within B158DRAFT_1491 at 1548.382 kb on - strand, within B158DRAFT_1491 at 1548.583 kb on - strand, within B158DRAFT_1491 at 1548.588 kb on - strand, within B158DRAFT_1491 at 1548.835 kb on + strand at 1548.862 kb on + strand at 1548.862 kb on + strand at 1548.911 kb on + strand at 1548.918 kb on + strand at 1548.918 kb on + strand at 1548.926 kb on - strand at 1548.926 kb on - strand at 1548.926 kb on - strand at 1548.926 kb on - strand at 1548.926 kb on - strand at 1548.926 kb on - strand at 1548.926 kb on - strand at 1549.152 kb on + strand, within B158DRAFT_1492 at 1549.160 kb on - strand, within B158DRAFT_1492 at 1549.160 kb on - strand, within B158DRAFT_1492 at 1549.186 kb on - strand, within B158DRAFT_1492 at 1549.186 kb on - strand, within B158DRAFT_1492 at 1549.186 kb on - strand, within B158DRAFT_1492 at 1549.186 kb on - strand, within B158DRAFT_1492 at 1549.186 kb on - strand, within B158DRAFT_1492 at 1549.186 kb on - strand, within B158DRAFT_1492 at 1549.186 kb on - strand, within B158DRAFT_1492 at 1549.186 kb on - strand, within B158DRAFT_1492 at 1549.186 kb on - strand, within B158DRAFT_1492 at 1549.219 kb on - strand, within B158DRAFT_1492 at 1549.294 kb on - strand, within B158DRAFT_1492 at 1549.313 kb on + strand, within B158DRAFT_1492 at 1549.329 kb on - strand, within B158DRAFT_1492 at 1549.408 kb on - strand, within B158DRAFT_1492 at 1549.456 kb on + strand, within B158DRAFT_1492 at 1549.456 kb on + strand, within B158DRAFT_1492 at 1549.466 kb on - strand, within B158DRAFT_1492 at 1549.466 kb on - strand, within B158DRAFT_1492 at 1549.536 kb on + strand, within B158DRAFT_1492 at 1549.536 kb on + strand, within B158DRAFT_1492 at 1549.544 kb on - strand, within B158DRAFT_1492 at 1549.544 kb on - strand, within B158DRAFT_1492 at 1549.544 kb on - strand, within B158DRAFT_1492 at 1549.544 kb on - strand, within B158DRAFT_1492 at 1549.544 kb on - strand, within B158DRAFT_1492 at 1549.563 kb on - strand, within B158DRAFT_1492 at 1549.577 kb on - strand, within B158DRAFT_1492 at 1549.608 kb on - strand, within B158DRAFT_1492 at 1549.668 kb on + strand, within B158DRAFT_1492 at 1549.706 kb on - strand, within B158DRAFT_1492 at 1549.745 kb on - strand, within B158DRAFT_1492 at 1549.769 kb on + strand, within B158DRAFT_1492 at 1549.774 kb on + strand, within B158DRAFT_1492 at 1549.775 kb on - strand, within B158DRAFT_1492 at 1549.775 kb on - strand, within B158DRAFT_1492 at 1549.775 kb on - strand, within B158DRAFT_1492 at 1549.776 kb on + strand, within B158DRAFT_1492 at 1549.777 kb on - strand, within B158DRAFT_1492 at 1549.782 kb on - strand, within B158DRAFT_1492 at 1549.792 kb on + strand, within B158DRAFT_1492 at 1549.792 kb on + strand, within B158DRAFT_1492 at 1549.800 kb on - strand, within B158DRAFT_1492 at 1549.800 kb on - strand, within B158DRAFT_1492 at 1549.800 kb on - strand, within B158DRAFT_1492 at 1549.800 kb on - strand, within B158DRAFT_1492 at 1549.800 kb on - strand, within B158DRAFT_1492 at 1549.800 kb on - strand, within B158DRAFT_1492 at 1549.854 kb on + strand, within B158DRAFT_1492 at 1549.854 kb on + strand, within B158DRAFT_1492 at 1549.857 kb on - strand, within B158DRAFT_1492 at 1549.862 kb on - strand, within B158DRAFT_1492 at 1549.862 kb on - strand, within B158DRAFT_1492 at 1549.862 kb on - strand, within B158DRAFT_1492 at 1549.862 kb on - strand, within B158DRAFT_1492 at 1549.862 kb on - strand, within B158DRAFT_1492 at 1549.871 kb on + strand, within B158DRAFT_1492 at 1549.883 kb on + strand, within B158DRAFT_1492 at 1549.883 kb on + strand, within B158DRAFT_1492 at 1549.883 kb on + strand, within B158DRAFT_1492 at 1549.883 kb on + strand, within B158DRAFT_1492 at 1549.883 kb on + strand, within B158DRAFT_1492 at 1549.883 kb on + strand, within B158DRAFT_1492 at 1549.891 kb on - strand, within B158DRAFT_1492 at 1549.891 kb on - strand, within B158DRAFT_1492 at 1549.891 kb on - strand, within B158DRAFT_1492 at 1549.891 kb on - strand, within B158DRAFT_1492 at 1549.891 kb on - strand, within B158DRAFT_1492 at 1549.891 kb on - strand, within B158DRAFT_1492 at 1549.902 kb on + strand, within B158DRAFT_1492 at 1550.041 kb on + strand, within B158DRAFT_1492 at 1550.041 kb on + strand, within B158DRAFT_1492 at 1550.041 kb on + strand, within B158DRAFT_1492 at 1550.041 kb on + strand, within B158DRAFT_1492 at 1550.041 kb on + strand, within B158DRAFT_1492 at 1550.041 kb on + strand, within B158DRAFT_1492 at 1550.049 kb on - strand, within B158DRAFT_1492 at 1550.049 kb on - strand, within B158DRAFT_1492 at 1550.049 kb on - strand, within B158DRAFT_1492 at 1550.070 kb on - strand, within B158DRAFT_1492 at 1550.171 kb on + strand at 1550.171 kb on + strand at 1550.179 kb on - strand at 1550.179 kb on - strand at 1550.179 kb on - strand at 1550.179 kb on - strand at 1550.222 kb on - strand at 1550.325 kb on - strand at 1550.352 kb on + strand at 1550.395 kb on - strand at 1550.534 kb on - strand, within B158DRAFT_1493 at 1550.534 kb on - strand, within B158DRAFT_1493 at 1550.541 kb on - strand, within B158DRAFT_1493 at 1550.554 kb on + strand, within B158DRAFT_1493 at 1550.618 kb on + strand at 1550.626 kb on - strand at 1550.635 kb on - strand at 1550.640 kb on + strand at 1550.648 kb on - strand at 1550.688 kb on + strand at 1550.767 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Nickel (II) chloride 1.25 mM remove 1,548,042 + +0.2 1,548,085 + +0.3 1,548,093 - +0.1 1,548,095 + +0.5 1,548,103 - +0.3 1,548,118 - +0.3 1,548,130 - +0.3 1,548,181 + B158DRAFT_1491 0.11 +0.9 1,548,208 + B158DRAFT_1491 0.15 +0.2 1,548,234 - B158DRAFT_1491 0.18 +0.7 1,548,234 - B158DRAFT_1491 0.18 +1.0 1,548,244 + B158DRAFT_1491 0.19 +0.0 1,548,252 - B158DRAFT_1491 0.20 +0.0 1,548,257 - B158DRAFT_1491 0.21 +0.4 1,548,288 - B158DRAFT_1491 0.25 +0.3 1,548,382 - B158DRAFT_1491 0.37 +0.2 1,548,583 - B158DRAFT_1491 0.62 -0.3 1,548,588 - B158DRAFT_1491 0.63 +0.7 1,548,835 + -0.8 1,548,862 + +0.2 1,548,862 + -0.3 1,548,911 + -1.0 1,548,918 + -0.3 1,548,918 + -1.3 1,548,926 - -2.3 1,548,926 - -0.5 1,548,926 - -2.3 1,548,926 - -0.8 1,548,926 - -0.6 1,548,926 - -1.6 1,548,926 - -0.8 1,549,152 + B158DRAFT_1492 0.20 +0.7 1,549,160 - B158DRAFT_1492 0.20 +0.0 1,549,160 - B158DRAFT_1492 0.20 -1.3 1,549,186 - B158DRAFT_1492 0.22 -0.7 1,549,186 - B158DRAFT_1492 0.22 -2.3 1,549,186 - B158DRAFT_1492 0.22 +0.1 1,549,186 - B158DRAFT_1492 0.22 -2.0 1,549,186 - B158DRAFT_1492 0.22 -0.4 1,549,186 - B158DRAFT_1492 0.22 -1.0 1,549,186 - B158DRAFT_1492 0.22 -2.1 1,549,186 - B158DRAFT_1492 0.22 -0.3 1,549,186 - B158DRAFT_1492 0.22 -1.1 1,549,219 - B158DRAFT_1492 0.24 -0.8 1,549,294 - B158DRAFT_1492 0.30 -1.2 1,549,313 + B158DRAFT_1492 0.31 -1.7 1,549,329 - B158DRAFT_1492 0.32 -0.8 1,549,408 - B158DRAFT_1492 0.38 -2.0 1,549,456 + B158DRAFT_1492 0.41 -0.1 1,549,456 + B158DRAFT_1492 0.41 -0.0 1,549,466 - B158DRAFT_1492 0.42 -1.3 1,549,466 - B158DRAFT_1492 0.42 -1.7 1,549,536 + B158DRAFT_1492 0.47 -1.1 1,549,536 + B158DRAFT_1492 0.47 +1.2 1,549,544 - B158DRAFT_1492 0.48 +0.3 1,549,544 - B158DRAFT_1492 0.48 -0.1 1,549,544 - B158DRAFT_1492 0.48 -0.2 1,549,544 - B158DRAFT_1492 0.48 -0.1 1,549,544 - B158DRAFT_1492 0.48 -0.3 1,549,563 - B158DRAFT_1492 0.49 -1.6 1,549,577 - B158DRAFT_1492 0.50 -1.2 1,549,608 - B158DRAFT_1492 0.52 -2.6 1,549,668 + B158DRAFT_1492 0.57 -0.8 1,549,706 - B158DRAFT_1492 0.59 -0.4 1,549,745 - B158DRAFT_1492 0.62 -0.1 1,549,769 + B158DRAFT_1492 0.64 -0.3 1,549,774 + B158DRAFT_1492 0.64 +1.3 1,549,775 - B158DRAFT_1492 0.64 -0.4 1,549,775 - B158DRAFT_1492 0.64 -1.2 1,549,775 - B158DRAFT_1492 0.64 -1.7 1,549,776 + B158DRAFT_1492 0.64 -0.6 1,549,777 - B158DRAFT_1492 0.64 -1.7 1,549,782 - B158DRAFT_1492 0.65 -1.2 1,549,792 + B158DRAFT_1492 0.66 +0.5 1,549,792 + B158DRAFT_1492 0.66 -0.7 1,549,800 - B158DRAFT_1492 0.66 -0.6 1,549,800 - B158DRAFT_1492 0.66 -0.7 1,549,800 - B158DRAFT_1492 0.66 -1.0 1,549,800 - B158DRAFT_1492 0.66 -1.2 1,549,800 - B158DRAFT_1492 0.66 -1.0 1,549,800 - B158DRAFT_1492 0.66 -1.4 1,549,854 + B158DRAFT_1492 0.70 -2.3 1,549,854 + B158DRAFT_1492 0.70 +0.6 1,549,857 - B158DRAFT_1492 0.70 -2.4 1,549,862 - B158DRAFT_1492 0.71 -1.6 1,549,862 - B158DRAFT_1492 0.71 +1.0 1,549,862 - B158DRAFT_1492 0.71 +0.4 1,549,862 - B158DRAFT_1492 0.71 +0.0 1,549,862 - B158DRAFT_1492 0.71 -0.0 1,549,871 + B158DRAFT_1492 0.71 -1.8 1,549,883 + B158DRAFT_1492 0.72 -0.5 1,549,883 + B158DRAFT_1492 0.72 +0.3 1,549,883 + B158DRAFT_1492 0.72 -0.3 1,549,883 + B158DRAFT_1492 0.72 -1.8 1,549,883 + B158DRAFT_1492 0.72 +2.2 1,549,883 + B158DRAFT_1492 0.72 -0.8 1,549,891 - B158DRAFT_1492 0.73 -1.8 1,549,891 - B158DRAFT_1492 0.73 -1.2 1,549,891 - B158DRAFT_1492 0.73 -0.3 1,549,891 - B158DRAFT_1492 0.73 -4.1 1,549,891 - B158DRAFT_1492 0.73 -1.5 1,549,891 - B158DRAFT_1492 0.73 -0.2 1,549,902 + B158DRAFT_1492 0.73 -0.0 1,550,041 + B158DRAFT_1492 0.83 +0.7 1,550,041 + B158DRAFT_1492 0.83 -2.3 1,550,041 + B158DRAFT_1492 0.83 -0.9 1,550,041 + B158DRAFT_1492 0.83 +0.2 1,550,041 + B158DRAFT_1492 0.83 +0.1 1,550,041 + B158DRAFT_1492 0.83 -0.6 1,550,049 - B158DRAFT_1492 0.84 -1.6 1,550,049 - B158DRAFT_1492 0.84 -0.8 1,550,049 - B158DRAFT_1492 0.84 -1.0 1,550,070 - B158DRAFT_1492 0.85 -1.4 1,550,171 + -0.7 1,550,171 + -2.0 1,550,179 - -0.8 1,550,179 - -0.7 1,550,179 - -1.3 1,550,179 - -1.0 1,550,222 - -0.3 1,550,325 - -0.9 1,550,352 + +0.0 1,550,395 - +0.4 1,550,534 - B158DRAFT_1493 0.32 +0.0 1,550,534 - B158DRAFT_1493 0.32 +0.3 1,550,541 - B158DRAFT_1493 0.41 -0.1 1,550,554 + B158DRAFT_1493 0.58 -0.8 1,550,618 + -0.0 1,550,626 - -0.1 1,550,635 - +0.0 1,550,640 + -0.5 1,550,648 - -0.1 1,550,688 + +0.5 1,550,767 - -0.9
Or see this region's nucleotide sequence