Strain Fitness in Kangiella aquimarina DSM 16071 around B158DRAFT_1492

Experiment: m.b. Nickel (II) chloride 1.25 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntB158DRAFT_1490 and B158DRAFT_1491 are separated by 37 nucleotidesB158DRAFT_1491 and B158DRAFT_1492 overlap by 1 nucleotidesB158DRAFT_1492 and B158DRAFT_1493 are separated by 238 nucleotidesB158DRAFT_1493 and B158DRAFT_1494 are separated by 220 nucleotides B158DRAFT_1490: B158DRAFT_1490 - diguanylate cyclase (GGDEF) domain, at 1,544,677 to 1,548,054 _1490 B158DRAFT_1491: B158DRAFT_1491 - hypothetical protein, at 1,548,092 to 1,548,880 _1491 B158DRAFT_1492: B158DRAFT_1492 - FAD/FMN-containing dehydrogenases, at 1,548,880 to 1,550,271 _1492 B158DRAFT_1493: B158DRAFT_1493 - tRNA_Val_TAC, at 1,550,510 to 1,550,585 _1493 B158DRAFT_1494: B158DRAFT_1494 - glutamyl-tRNA synthetase (EC 6.1.1.17), at 1,550,806 to 1,552,224 _1494 Position (kb) 1548 1549 1550 1551Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1548.042 kb on + strandat 1548.085 kb on + strandat 1548.093 kb on - strandat 1548.095 kb on + strandat 1548.103 kb on - strandat 1548.118 kb on - strandat 1548.130 kb on - strandat 1548.181 kb on + strand, within B158DRAFT_1491at 1548.208 kb on + strand, within B158DRAFT_1491at 1548.234 kb on - strand, within B158DRAFT_1491at 1548.234 kb on - strand, within B158DRAFT_1491at 1548.244 kb on + strand, within B158DRAFT_1491at 1548.252 kb on - strand, within B158DRAFT_1491at 1548.257 kb on - strand, within B158DRAFT_1491at 1548.288 kb on - strand, within B158DRAFT_1491at 1548.382 kb on - strand, within B158DRAFT_1491at 1548.583 kb on - strand, within B158DRAFT_1491at 1548.588 kb on - strand, within B158DRAFT_1491at 1548.835 kb on + strandat 1548.862 kb on + strandat 1548.862 kb on + strandat 1548.911 kb on + strandat 1548.918 kb on + strandat 1548.918 kb on + strandat 1548.926 kb on - strandat 1548.926 kb on - strandat 1548.926 kb on - strandat 1548.926 kb on - strandat 1548.926 kb on - strandat 1548.926 kb on - strandat 1548.926 kb on - strandat 1549.152 kb on + strand, within B158DRAFT_1492at 1549.160 kb on - strand, within B158DRAFT_1492at 1549.160 kb on - strand, within B158DRAFT_1492at 1549.186 kb on - strand, within B158DRAFT_1492at 1549.186 kb on - strand, within B158DRAFT_1492at 1549.186 kb on - strand, within B158DRAFT_1492at 1549.186 kb on - strand, within B158DRAFT_1492at 1549.186 kb on - strand, within B158DRAFT_1492at 1549.186 kb on - strand, within B158DRAFT_1492at 1549.186 kb on - strand, within B158DRAFT_1492at 1549.186 kb on - strand, within B158DRAFT_1492at 1549.186 kb on - strand, within B158DRAFT_1492at 1549.219 kb on - strand, within B158DRAFT_1492at 1549.294 kb on - strand, within B158DRAFT_1492at 1549.313 kb on + strand, within B158DRAFT_1492at 1549.329 kb on - strand, within B158DRAFT_1492at 1549.408 kb on - strand, within B158DRAFT_1492at 1549.456 kb on + strand, within B158DRAFT_1492at 1549.456 kb on + strand, within B158DRAFT_1492at 1549.466 kb on - strand, within B158DRAFT_1492at 1549.466 kb on - strand, within B158DRAFT_1492at 1549.536 kb on + strand, within B158DRAFT_1492at 1549.536 kb on + strand, within B158DRAFT_1492at 1549.544 kb on - strand, within B158DRAFT_1492at 1549.544 kb on - strand, within B158DRAFT_1492at 1549.544 kb on - strand, within B158DRAFT_1492at 1549.544 kb on - strand, within B158DRAFT_1492at 1549.544 kb on - strand, within B158DRAFT_1492at 1549.563 kb on - strand, within B158DRAFT_1492at 1549.577 kb on - strand, within B158DRAFT_1492at 1549.608 kb on - strand, within B158DRAFT_1492at 1549.668 kb on + strand, within B158DRAFT_1492at 1549.706 kb on - strand, within B158DRAFT_1492at 1549.745 kb on - strand, within B158DRAFT_1492at 1549.769 kb on + strand, within B158DRAFT_1492at 1549.774 kb on + strand, within B158DRAFT_1492at 1549.775 kb on - strand, within B158DRAFT_1492at 1549.775 kb on - strand, within B158DRAFT_1492at 1549.775 kb on - strand, within B158DRAFT_1492at 1549.776 kb on + strand, within B158DRAFT_1492at 1549.777 kb on - strand, within B158DRAFT_1492at 1549.782 kb on - strand, within B158DRAFT_1492at 1549.792 kb on + strand, within B158DRAFT_1492at 1549.792 kb on + strand, within B158DRAFT_1492at 1549.800 kb on - strand, within B158DRAFT_1492at 1549.800 kb on - strand, within B158DRAFT_1492at 1549.800 kb on - strand, within B158DRAFT_1492at 1549.800 kb on - strand, within B158DRAFT_1492at 1549.800 kb on - strand, within B158DRAFT_1492at 1549.800 kb on - strand, within B158DRAFT_1492at 1549.854 kb on + strand, within B158DRAFT_1492at 1549.854 kb on + strand, within B158DRAFT_1492at 1549.857 kb on - strand, within B158DRAFT_1492at 1549.862 kb on - strand, within B158DRAFT_1492at 1549.862 kb on - strand, within B158DRAFT_1492at 1549.862 kb on - strand, within B158DRAFT_1492at 1549.862 kb on - strand, within B158DRAFT_1492at 1549.862 kb on - strand, within B158DRAFT_1492at 1549.871 kb on + strand, within B158DRAFT_1492at 1549.883 kb on + strand, within B158DRAFT_1492at 1549.883 kb on + strand, within B158DRAFT_1492at 1549.883 kb on + strand, within B158DRAFT_1492at 1549.883 kb on + strand, within B158DRAFT_1492at 1549.883 kb on + strand, within B158DRAFT_1492at 1549.883 kb on + strand, within B158DRAFT_1492at 1549.891 kb on - strand, within B158DRAFT_1492at 1549.891 kb on - strand, within B158DRAFT_1492at 1549.891 kb on - strand, within B158DRAFT_1492at 1549.891 kb on - strand, within B158DRAFT_1492at 1549.891 kb on - strand, within B158DRAFT_1492at 1549.891 kb on - strand, within B158DRAFT_1492at 1549.902 kb on + strand, within B158DRAFT_1492at 1550.041 kb on + strand, within B158DRAFT_1492at 1550.041 kb on + strand, within B158DRAFT_1492at 1550.041 kb on + strand, within B158DRAFT_1492at 1550.041 kb on + strand, within B158DRAFT_1492at 1550.041 kb on + strand, within B158DRAFT_1492at 1550.041 kb on + strand, within B158DRAFT_1492at 1550.049 kb on - strand, within B158DRAFT_1492at 1550.049 kb on - strand, within B158DRAFT_1492at 1550.049 kb on - strand, within B158DRAFT_1492at 1550.070 kb on - strand, within B158DRAFT_1492at 1550.171 kb on + strandat 1550.171 kb on + strandat 1550.179 kb on - strandat 1550.179 kb on - strandat 1550.179 kb on - strandat 1550.179 kb on - strandat 1550.222 kb on - strandat 1550.325 kb on - strandat 1550.352 kb on + strandat 1550.395 kb on - strandat 1550.534 kb on - strand, within B158DRAFT_1493at 1550.534 kb on - strand, within B158DRAFT_1493at 1550.541 kb on - strand, within B158DRAFT_1493at 1550.554 kb on + strand, within B158DRAFT_1493at 1550.618 kb on + strandat 1550.626 kb on - strandat 1550.635 kb on - strandat 1550.640 kb on + strandat 1550.648 kb on - strandat 1550.688 kb on + strandat 1550.767 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction m.b. Nickel (II) chloride 1.25 mM
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1,548,042 + +0.2
1,548,085 + +0.3
1,548,093 - +0.1
1,548,095 + +0.5
1,548,103 - +0.3
1,548,118 - +0.3
1,548,130 - +0.3
1,548,181 + B158DRAFT_1491 0.11 +0.9
1,548,208 + B158DRAFT_1491 0.15 +0.2
1,548,234 - B158DRAFT_1491 0.18 +0.7
1,548,234 - B158DRAFT_1491 0.18 +1.0
1,548,244 + B158DRAFT_1491 0.19 +0.0
1,548,252 - B158DRAFT_1491 0.20 +0.0
1,548,257 - B158DRAFT_1491 0.21 +0.4
1,548,288 - B158DRAFT_1491 0.25 +0.3
1,548,382 - B158DRAFT_1491 0.37 +0.2
1,548,583 - B158DRAFT_1491 0.62 -0.3
1,548,588 - B158DRAFT_1491 0.63 +0.7
1,548,835 + -0.8
1,548,862 + +0.2
1,548,862 + -0.3
1,548,911 + -1.0
1,548,918 + -0.3
1,548,918 + -1.3
1,548,926 - -2.3
1,548,926 - -0.5
1,548,926 - -2.3
1,548,926 - -0.8
1,548,926 - -0.6
1,548,926 - -1.6
1,548,926 - -0.8
1,549,152 + B158DRAFT_1492 0.20 +0.7
1,549,160 - B158DRAFT_1492 0.20 +0.0
1,549,160 - B158DRAFT_1492 0.20 -1.3
1,549,186 - B158DRAFT_1492 0.22 -0.7
1,549,186 - B158DRAFT_1492 0.22 -2.3
1,549,186 - B158DRAFT_1492 0.22 +0.1
1,549,186 - B158DRAFT_1492 0.22 -2.0
1,549,186 - B158DRAFT_1492 0.22 -0.4
1,549,186 - B158DRAFT_1492 0.22 -1.0
1,549,186 - B158DRAFT_1492 0.22 -2.1
1,549,186 - B158DRAFT_1492 0.22 -0.3
1,549,186 - B158DRAFT_1492 0.22 -1.1
1,549,219 - B158DRAFT_1492 0.24 -0.8
1,549,294 - B158DRAFT_1492 0.30 -1.2
1,549,313 + B158DRAFT_1492 0.31 -1.7
1,549,329 - B158DRAFT_1492 0.32 -0.8
1,549,408 - B158DRAFT_1492 0.38 -2.0
1,549,456 + B158DRAFT_1492 0.41 -0.1
1,549,456 + B158DRAFT_1492 0.41 -0.0
1,549,466 - B158DRAFT_1492 0.42 -1.3
1,549,466 - B158DRAFT_1492 0.42 -1.7
1,549,536 + B158DRAFT_1492 0.47 -1.1
1,549,536 + B158DRAFT_1492 0.47 +1.2
1,549,544 - B158DRAFT_1492 0.48 +0.3
1,549,544 - B158DRAFT_1492 0.48 -0.1
1,549,544 - B158DRAFT_1492 0.48 -0.2
1,549,544 - B158DRAFT_1492 0.48 -0.1
1,549,544 - B158DRAFT_1492 0.48 -0.3
1,549,563 - B158DRAFT_1492 0.49 -1.6
1,549,577 - B158DRAFT_1492 0.50 -1.2
1,549,608 - B158DRAFT_1492 0.52 -2.6
1,549,668 + B158DRAFT_1492 0.57 -0.8
1,549,706 - B158DRAFT_1492 0.59 -0.4
1,549,745 - B158DRAFT_1492 0.62 -0.1
1,549,769 + B158DRAFT_1492 0.64 -0.3
1,549,774 + B158DRAFT_1492 0.64 +1.3
1,549,775 - B158DRAFT_1492 0.64 -0.4
1,549,775 - B158DRAFT_1492 0.64 -1.2
1,549,775 - B158DRAFT_1492 0.64 -1.7
1,549,776 + B158DRAFT_1492 0.64 -0.6
1,549,777 - B158DRAFT_1492 0.64 -1.7
1,549,782 - B158DRAFT_1492 0.65 -1.2
1,549,792 + B158DRAFT_1492 0.66 +0.5
1,549,792 + B158DRAFT_1492 0.66 -0.7
1,549,800 - B158DRAFT_1492 0.66 -0.6
1,549,800 - B158DRAFT_1492 0.66 -0.7
1,549,800 - B158DRAFT_1492 0.66 -1.0
1,549,800 - B158DRAFT_1492 0.66 -1.2
1,549,800 - B158DRAFT_1492 0.66 -1.0
1,549,800 - B158DRAFT_1492 0.66 -1.4
1,549,854 + B158DRAFT_1492 0.70 -2.3
1,549,854 + B158DRAFT_1492 0.70 +0.6
1,549,857 - B158DRAFT_1492 0.70 -2.4
1,549,862 - B158DRAFT_1492 0.71 -1.6
1,549,862 - B158DRAFT_1492 0.71 +1.0
1,549,862 - B158DRAFT_1492 0.71 +0.4
1,549,862 - B158DRAFT_1492 0.71 +0.0
1,549,862 - B158DRAFT_1492 0.71 -0.0
1,549,871 + B158DRAFT_1492 0.71 -1.8
1,549,883 + B158DRAFT_1492 0.72 -0.5
1,549,883 + B158DRAFT_1492 0.72 +0.3
1,549,883 + B158DRAFT_1492 0.72 -0.3
1,549,883 + B158DRAFT_1492 0.72 -1.8
1,549,883 + B158DRAFT_1492 0.72 +2.2
1,549,883 + B158DRAFT_1492 0.72 -0.8
1,549,891 - B158DRAFT_1492 0.73 -1.8
1,549,891 - B158DRAFT_1492 0.73 -1.2
1,549,891 - B158DRAFT_1492 0.73 -0.3
1,549,891 - B158DRAFT_1492 0.73 -4.1
1,549,891 - B158DRAFT_1492 0.73 -1.5
1,549,891 - B158DRAFT_1492 0.73 -0.2
1,549,902 + B158DRAFT_1492 0.73 -0.0
1,550,041 + B158DRAFT_1492 0.83 +0.7
1,550,041 + B158DRAFT_1492 0.83 -2.3
1,550,041 + B158DRAFT_1492 0.83 -0.9
1,550,041 + B158DRAFT_1492 0.83 +0.2
1,550,041 + B158DRAFT_1492 0.83 +0.1
1,550,041 + B158DRAFT_1492 0.83 -0.6
1,550,049 - B158DRAFT_1492 0.84 -1.6
1,550,049 - B158DRAFT_1492 0.84 -0.8
1,550,049 - B158DRAFT_1492 0.84 -1.0
1,550,070 - B158DRAFT_1492 0.85 -1.4
1,550,171 + -0.7
1,550,171 + -2.0
1,550,179 - -0.8
1,550,179 - -0.7
1,550,179 - -1.3
1,550,179 - -1.0
1,550,222 - -0.3
1,550,325 - -0.9
1,550,352 + +0.0
1,550,395 - +0.4
1,550,534 - B158DRAFT_1493 0.32 +0.0
1,550,534 - B158DRAFT_1493 0.32 +0.3
1,550,541 - B158DRAFT_1493 0.41 -0.1
1,550,554 + B158DRAFT_1493 0.58 -0.8
1,550,618 + -0.0
1,550,626 - -0.1
1,550,635 - +0.0
1,550,640 + -0.5
1,550,648 - -0.1
1,550,688 + +0.5
1,550,767 - -0.9

Or see this region's nucleotide sequence