Experiment: m.b. nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_1475 and B158DRAFT_1476 overlap by 4 nucleotides B158DRAFT_1476 and B158DRAFT_1477 overlap by 1 nucleotides B158DRAFT_1477 and B158DRAFT_1478 are separated by 419 nucleotides B158DRAFT_1478 and B158DRAFT_1479 are separated by 122 nucleotides
B158DRAFT_1475: B158DRAFT_1475 - Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes, at 1,526,699 to 1,528,417
_1475
B158DRAFT_1476: B158DRAFT_1476 - Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit, at 1,528,414 to 1,529,394
_1476
B158DRAFT_1477: B158DRAFT_1477 - pyruvate dehydrogenase E1 component, alpha subunit, at 1,529,394 to 1,530,485
_1477
B158DRAFT_1478: B158DRAFT_1478 - hypothetical protein, at 1,530,905 to 1,531,288
_1478
B158DRAFT_1479: B158DRAFT_1479 - aminopeptidase N, Escherichia coli type, at 1,531,411 to 1,534,077
_1479
Position (kb)
1529
1530
1531 Strain fitness (log2 ratio)
-1
0
1 at 1529.421 kb on - strand at 1530.172 kb on + strand, within B158DRAFT_1477 at 1530.503 kb on - strand at 1530.503 kb on - strand at 1530.503 kb on - strand at 1530.503 kb on - strand at 1530.503 kb on - strand at 1530.503 kb on - strand at 1530.503 kb on - strand at 1530.503 kb on - strand at 1530.503 kb on - strand at 1530.503 kb on - strand at 1530.534 kb on - strand at 1530.704 kb on - strand at 1530.704 kb on - strand at 1530.777 kb on + strand at 1530.785 kb on - strand at 1530.958 kb on + strand, within B158DRAFT_1478 at 1530.966 kb on - strand, within B158DRAFT_1478 at 1530.968 kb on + strand, within B158DRAFT_1478 at 1530.968 kb on + strand, within B158DRAFT_1478 at 1530.982 kb on + strand, within B158DRAFT_1478 at 1530.990 kb on - strand, within B158DRAFT_1478 at 1531.004 kb on + strand, within B158DRAFT_1478 at 1531.004 kb on + strand, within B158DRAFT_1478 at 1531.004 kb on + strand, within B158DRAFT_1478 at 1531.012 kb on - strand, within B158DRAFT_1478 at 1531.012 kb on - strand, within B158DRAFT_1478 at 1531.012 kb on - strand, within B158DRAFT_1478 at 1531.012 kb on - strand, within B158DRAFT_1478 at 1531.012 kb on - strand, within B158DRAFT_1478 at 1531.012 kb on - strand, within B158DRAFT_1478 at 1531.012 kb on - strand, within B158DRAFT_1478 at 1531.012 kb on - strand, within B158DRAFT_1478 at 1531.012 kb on - strand, within B158DRAFT_1478 at 1531.012 kb on - strand, within B158DRAFT_1478 at 1531.012 kb on - strand, within B158DRAFT_1478 at 1531.045 kb on + strand, within B158DRAFT_1478 at 1531.087 kb on + strand, within B158DRAFT_1478 at 1531.108 kb on + strand, within B158DRAFT_1478 at 1531.148 kb on + strand, within B158DRAFT_1478 at 1531.159 kb on + strand, within B158DRAFT_1478 at 1531.159 kb on + strand, within B158DRAFT_1478 at 1531.167 kb on - strand, within B158DRAFT_1478 at 1531.167 kb on - strand, within B158DRAFT_1478 at 1531.167 kb on - strand, within B158DRAFT_1478 at 1531.167 kb on - strand, within B158DRAFT_1478 at 1531.190 kb on - strand, within B158DRAFT_1478 at 1531.190 kb on - strand, within B158DRAFT_1478 at 1531.194 kb on + strand, within B158DRAFT_1478 at 1531.194 kb on + strand, within B158DRAFT_1478 at 1531.194 kb on + strand, within B158DRAFT_1478 at 1531.194 kb on + strand, within B158DRAFT_1478 at 1531.198 kb on + strand, within B158DRAFT_1478 at 1531.202 kb on - strand, within B158DRAFT_1478 at 1531.202 kb on - strand, within B158DRAFT_1478 at 1531.202 kb on - strand, within B158DRAFT_1478 at 1531.202 kb on - strand, within B158DRAFT_1478 at 1531.203 kb on + strand, within B158DRAFT_1478 at 1531.217 kb on + strand, within B158DRAFT_1478 at 1531.217 kb on + strand, within B158DRAFT_1478 at 1531.271 kb on + strand at 1531.278 kb on + strand at 1531.286 kb on - strand at 1531.290 kb on + strand at 1531.441 kb on - strand at 1531.450 kb on + strand at 1531.458 kb on - strand at 1531.458 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. nitrate 200 mM remove 1,529,421 - +0.1 1,530,172 + B158DRAFT_1477 0.71 -1.0 1,530,503 - -0.3 1,530,503 - -0.6 1,530,503 - +0.6 1,530,503 - -0.9 1,530,503 - -0.9 1,530,503 - +0.2 1,530,503 - +0.3 1,530,503 - +0.8 1,530,503 - -0.3 1,530,503 - +1.2 1,530,534 - -0.0 1,530,704 - -0.1 1,530,704 - +0.4 1,530,777 + +0.4 1,530,785 - +0.0 1,530,958 + B158DRAFT_1478 0.14 -0.3 1,530,966 - B158DRAFT_1478 0.16 +0.3 1,530,968 + B158DRAFT_1478 0.16 -1.2 1,530,968 + B158DRAFT_1478 0.16 -0.3 1,530,982 + B158DRAFT_1478 0.20 -0.4 1,530,990 - B158DRAFT_1478 0.22 -0.8 1,531,004 + B158DRAFT_1478 0.26 -0.9 1,531,004 + B158DRAFT_1478 0.26 +0.4 1,531,004 + B158DRAFT_1478 0.26 -0.2 1,531,012 - B158DRAFT_1478 0.28 -0.2 1,531,012 - B158DRAFT_1478 0.28 -0.5 1,531,012 - B158DRAFT_1478 0.28 -0.3 1,531,012 - B158DRAFT_1478 0.28 -0.2 1,531,012 - B158DRAFT_1478 0.28 -0.5 1,531,012 - B158DRAFT_1478 0.28 -0.4 1,531,012 - B158DRAFT_1478 0.28 +0.4 1,531,012 - B158DRAFT_1478 0.28 +0.1 1,531,012 - B158DRAFT_1478 0.28 +0.2 1,531,012 - B158DRAFT_1478 0.28 -0.0 1,531,012 - B158DRAFT_1478 0.28 -0.5 1,531,045 + B158DRAFT_1478 0.36 -0.1 1,531,087 + B158DRAFT_1478 0.47 -0.9 1,531,108 + B158DRAFT_1478 0.53 -0.3 1,531,148 + B158DRAFT_1478 0.63 +0.5 1,531,159 + B158DRAFT_1478 0.66 -0.3 1,531,159 + B158DRAFT_1478 0.66 +0.4 1,531,167 - B158DRAFT_1478 0.68 +0.3 1,531,167 - B158DRAFT_1478 0.68 +0.0 1,531,167 - B158DRAFT_1478 0.68 +0.2 1,531,167 - B158DRAFT_1478 0.68 +0.1 1,531,190 - B158DRAFT_1478 0.74 -0.2 1,531,190 - B158DRAFT_1478 0.74 +0.9 1,531,194 + B158DRAFT_1478 0.75 +0.2 1,531,194 + B158DRAFT_1478 0.75 -0.5 1,531,194 + B158DRAFT_1478 0.75 -0.6 1,531,194 + B158DRAFT_1478 0.75 -0.2 1,531,198 + B158DRAFT_1478 0.76 -0.0 1,531,202 - B158DRAFT_1478 0.77 -0.0 1,531,202 - B158DRAFT_1478 0.77 -0.3 1,531,202 - B158DRAFT_1478 0.77 -0.6 1,531,202 - B158DRAFT_1478 0.77 -0.4 1,531,203 + B158DRAFT_1478 0.78 -0.8 1,531,217 + B158DRAFT_1478 0.81 -0.2 1,531,217 + B158DRAFT_1478 0.81 +0.2 1,531,271 + -0.1 1,531,278 + -1.7 1,531,286 - -0.3 1,531,290 + -0.6 1,531,441 - -0.1 1,531,450 + +0.1 1,531,458 - +0.3 1,531,458 - -0.0
Or see this region's nucleotide sequence