Experiment: m.b. nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_0003 and B158DRAFT_0004 are separated by 6 nucleotides B158DRAFT_0004 and B158DRAFT_0005 are separated by 80 nucleotides
B158DRAFT_0003: B158DRAFT_0003 - Membrane proteins related to metalloendopeptidases, at 3,477 to 4,988
_0003
B158DRAFT_0004: B158DRAFT_0004 - Predicted molecular chaperone distantly related to HSP70-fold metalloproteases, at 4,995 to 6,101
_0004
B158DRAFT_0005: B158DRAFT_0005 - diguanylate cyclase (GGDEF) domain, at 6,182 to 7,309
_0005
Position (kb)
4
5
6
7 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4.191 kb on + strand, within B158DRAFT_0003 at 4.437 kb on - strand, within B158DRAFT_0003 at 4.477 kb on - strand, within B158DRAFT_0003 at 4.523 kb on - strand, within B158DRAFT_0003 at 4.595 kb on - strand, within B158DRAFT_0003 at 4.704 kb on + strand, within B158DRAFT_0003 at 4.714 kb on + strand, within B158DRAFT_0003 at 4.714 kb on + strand, within B158DRAFT_0003 at 4.845 kb on + strand at 4.878 kb on + strand at 4.967 kb on - strand at 5.038 kb on + strand at 5.175 kb on - strand, within B158DRAFT_0004 at 5.257 kb on + strand, within B158DRAFT_0004 at 5.290 kb on + strand, within B158DRAFT_0004 at 5.332 kb on - strand, within B158DRAFT_0004 at 5.355 kb on + strand, within B158DRAFT_0004 at 5.363 kb on - strand, within B158DRAFT_0004 at 5.417 kb on + strand, within B158DRAFT_0004 at 5.417 kb on + strand, within B158DRAFT_0004 at 5.435 kb on - strand, within B158DRAFT_0004 at 5.439 kb on + strand, within B158DRAFT_0004 at 5.439 kb on + strand, within B158DRAFT_0004 at 5.439 kb on + strand, within B158DRAFT_0004 at 5.444 kb on + strand, within B158DRAFT_0004 at 5.447 kb on - strand, within B158DRAFT_0004 at 5.481 kb on + strand, within B158DRAFT_0004 at 5.481 kb on + strand, within B158DRAFT_0004 at 5.489 kb on - strand, within B158DRAFT_0004 at 5.489 kb on - strand, within B158DRAFT_0004 at 5.517 kb on + strand, within B158DRAFT_0004 at 5.576 kb on - strand, within B158DRAFT_0004 at 5.610 kb on - strand, within B158DRAFT_0004 at 5.642 kb on - strand, within B158DRAFT_0004 at 5.703 kb on + strand, within B158DRAFT_0004 at 5.703 kb on + strand, within B158DRAFT_0004 at 5.711 kb on - strand, within B158DRAFT_0004 at 5.711 kb on - strand, within B158DRAFT_0004 at 5.736 kb on - strand, within B158DRAFT_0004 at 5.760 kb on - strand, within B158DRAFT_0004 at 5.857 kb on - strand, within B158DRAFT_0004 at 5.866 kb on + strand, within B158DRAFT_0004 at 5.868 kb on + strand, within B158DRAFT_0004 at 5.874 kb on - strand, within B158DRAFT_0004 at 5.874 kb on - strand, within B158DRAFT_0004 at 5.874 kb on - strand, within B158DRAFT_0004 at 5.876 kb on - strand, within B158DRAFT_0004 at 5.876 kb on - strand, within B158DRAFT_0004 at 5.876 kb on - strand, within B158DRAFT_0004 at 5.958 kb on - strand, within B158DRAFT_0004 at 5.970 kb on + strand, within B158DRAFT_0004 at 5.970 kb on + strand, within B158DRAFT_0004 at 5.978 kb on - strand, within B158DRAFT_0004 at 5.980 kb on + strand, within B158DRAFT_0004 at 5.988 kb on - strand, within B158DRAFT_0004 at 6.002 kb on - strand at 6.004 kb on + strand at 6.012 kb on - strand at 6.014 kb on + strand at 6.019 kb on + strand at 6.070 kb on + strand at 6.203 kb on - strand at 6.224 kb on + strand at 6.287 kb on + strand at 6.287 kb on + strand at 6.311 kb on + strand, within B158DRAFT_0005 at 6.330 kb on + strand, within B158DRAFT_0005 at 6.330 kb on + strand, within B158DRAFT_0005 at 6.330 kb on + strand, within B158DRAFT_0005 at 6.330 kb on + strand, within B158DRAFT_0005 at 6.330 kb on + strand, within B158DRAFT_0005 at 6.330 kb on + strand, within B158DRAFT_0005 at 6.330 kb on + strand, within B158DRAFT_0005 at 6.338 kb on - strand, within B158DRAFT_0005 at 6.338 kb on - strand, within B158DRAFT_0005 at 6.338 kb on - strand, within B158DRAFT_0005 at 6.338 kb on - strand, within B158DRAFT_0005 at 6.338 kb on - strand, within B158DRAFT_0005 at 6.338 kb on - strand, within B158DRAFT_0005 at 6.340 kb on + strand, within B158DRAFT_0005 at 6.421 kb on + strand, within B158DRAFT_0005 at 6.449 kb on + strand, within B158DRAFT_0005 at 6.449 kb on + strand, within B158DRAFT_0005 at 6.464 kb on + strand, within B158DRAFT_0005 at 6.464 kb on + strand, within B158DRAFT_0005 at 6.464 kb on + strand, within B158DRAFT_0005 at 6.464 kb on + strand, within B158DRAFT_0005 at 6.464 kb on - strand, within B158DRAFT_0005 at 6.464 kb on - strand, within B158DRAFT_0005 at 6.472 kb on - strand, within B158DRAFT_0005 at 6.472 kb on - strand, within B158DRAFT_0005 at 6.545 kb on + strand, within B158DRAFT_0005 at 6.545 kb on + strand, within B158DRAFT_0005 at 6.545 kb on + strand, within B158DRAFT_0005 at 6.553 kb on - strand, within B158DRAFT_0005 at 6.553 kb on - strand, within B158DRAFT_0005 at 6.553 kb on - strand, within B158DRAFT_0005 at 6.592 kb on - strand, within B158DRAFT_0005 at 6.664 kb on + strand, within B158DRAFT_0005 at 6.838 kb on - strand, within B158DRAFT_0005 at 6.962 kb on + strand, within B158DRAFT_0005 at 6.962 kb on + strand, within B158DRAFT_0005 at 6.970 kb on - strand, within B158DRAFT_0005 at 6.988 kb on + strand, within B158DRAFT_0005 at 7.051 kb on + strand, within B158DRAFT_0005 at 7.051 kb on + strand, within B158DRAFT_0005 at 7.051 kb on + strand, within B158DRAFT_0005 at 7.072 kb on - strand, within B158DRAFT_0005
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. nitrate 200 mM remove 4,191 + B158DRAFT_0003 0.47 +0.3 4,437 - B158DRAFT_0003 0.63 +0.3 4,477 - B158DRAFT_0003 0.66 -0.1 4,523 - B158DRAFT_0003 0.69 -1.6 4,595 - B158DRAFT_0003 0.74 -0.1 4,704 + B158DRAFT_0003 0.81 +0.1 4,714 + B158DRAFT_0003 0.82 -0.1 4,714 + B158DRAFT_0003 0.82 +0.2 4,845 + +0.8 4,878 + -1.1 4,967 - -1.6 5,038 + -2.7 5,175 - B158DRAFT_0004 0.16 -0.5 5,257 + B158DRAFT_0004 0.24 -2.8 5,290 + B158DRAFT_0004 0.27 -2.6 5,332 - B158DRAFT_0004 0.30 -0.8 5,355 + B158DRAFT_0004 0.33 -0.1 5,363 - B158DRAFT_0004 0.33 -1.1 5,417 + B158DRAFT_0004 0.38 -2.6 5,417 + B158DRAFT_0004 0.38 -1.9 5,435 - B158DRAFT_0004 0.40 -2.1 5,439 + B158DRAFT_0004 0.40 -3.8 5,439 + B158DRAFT_0004 0.40 -1.3 5,439 + B158DRAFT_0004 0.40 -2.1 5,444 + B158DRAFT_0004 0.41 -2.4 5,447 - B158DRAFT_0004 0.41 -1.9 5,481 + B158DRAFT_0004 0.44 -1.6 5,481 + B158DRAFT_0004 0.44 -1.6 5,489 - B158DRAFT_0004 0.45 -2.0 5,489 - B158DRAFT_0004 0.45 -2.1 5,517 + B158DRAFT_0004 0.47 -1.9 5,576 - B158DRAFT_0004 0.52 -2.0 5,610 - B158DRAFT_0004 0.56 -3.1 5,642 - B158DRAFT_0004 0.58 -1.2 5,703 + B158DRAFT_0004 0.64 -1.3 5,703 + B158DRAFT_0004 0.64 -2.0 5,711 - B158DRAFT_0004 0.65 -0.8 5,711 - B158DRAFT_0004 0.65 -2.5 5,736 - B158DRAFT_0004 0.67 -1.8 5,760 - B158DRAFT_0004 0.69 -3.3 5,857 - B158DRAFT_0004 0.78 -1.5 5,866 + B158DRAFT_0004 0.79 -1.1 5,868 + B158DRAFT_0004 0.79 -1.3 5,874 - B158DRAFT_0004 0.79 -3.1 5,874 - B158DRAFT_0004 0.79 -2.0 5,874 - B158DRAFT_0004 0.79 -1.5 5,876 - B158DRAFT_0004 0.80 -2.0 5,876 - B158DRAFT_0004 0.80 -4.3 5,876 - B158DRAFT_0004 0.80 -2.3 5,958 - B158DRAFT_0004 0.87 -1.2 5,970 + B158DRAFT_0004 0.88 -2.5 5,970 + B158DRAFT_0004 0.88 -1.9 5,978 - B158DRAFT_0004 0.89 -1.8 5,980 + B158DRAFT_0004 0.89 -1.8 5,988 - B158DRAFT_0004 0.90 -1.8 6,002 - -2.4 6,004 + -3.3 6,012 - -2.6 6,014 + -1.3 6,019 + -2.8 6,070 + -4.3 6,203 - -0.5 6,224 + -0.1 6,287 + -0.9 6,287 + -0.7 6,311 + B158DRAFT_0005 0.11 -0.3 6,330 + B158DRAFT_0005 0.13 -0.1 6,330 + B158DRAFT_0005 0.13 -1.1 6,330 + B158DRAFT_0005 0.13 +0.4 6,330 + B158DRAFT_0005 0.13 -0.1 6,330 + B158DRAFT_0005 0.13 -3.3 6,330 + B158DRAFT_0005 0.13 -0.4 6,330 + B158DRAFT_0005 0.13 -0.7 6,338 - B158DRAFT_0005 0.14 -0.3 6,338 - B158DRAFT_0005 0.14 -0.0 6,338 - B158DRAFT_0005 0.14 -0.2 6,338 - B158DRAFT_0005 0.14 +0.0 6,338 - B158DRAFT_0005 0.14 -0.5 6,338 - B158DRAFT_0005 0.14 -0.5 6,340 + B158DRAFT_0005 0.14 -0.3 6,421 + B158DRAFT_0005 0.21 +0.2 6,449 + B158DRAFT_0005 0.24 -0.1 6,449 + B158DRAFT_0005 0.24 +0.1 6,464 + B158DRAFT_0005 0.25 -0.2 6,464 + B158DRAFT_0005 0.25 -0.4 6,464 + B158DRAFT_0005 0.25 -0.0 6,464 + B158DRAFT_0005 0.25 -0.1 6,464 - B158DRAFT_0005 0.25 +0.3 6,464 - B158DRAFT_0005 0.25 -0.4 6,472 - B158DRAFT_0005 0.26 -0.1 6,472 - B158DRAFT_0005 0.26 -0.1 6,545 + B158DRAFT_0005 0.32 +0.0 6,545 + B158DRAFT_0005 0.32 -1.1 6,545 + B158DRAFT_0005 0.32 -0.1 6,553 - B158DRAFT_0005 0.33 +0.4 6,553 - B158DRAFT_0005 0.33 -0.0 6,553 - B158DRAFT_0005 0.33 +0.0 6,592 - B158DRAFT_0005 0.36 -0.2 6,664 + B158DRAFT_0005 0.43 +0.2 6,838 - B158DRAFT_0005 0.58 +0.2 6,962 + B158DRAFT_0005 0.69 -0.3 6,962 + B158DRAFT_0005 0.69 -0.1 6,970 - B158DRAFT_0005 0.70 +0.9 6,988 + B158DRAFT_0005 0.71 -0.3 7,051 + B158DRAFT_0005 0.77 -0.4 7,051 + B158DRAFT_0005 0.77 +0.2 7,051 + B158DRAFT_0005 0.77 -0.5 7,072 - B158DRAFT_0005 0.79 -0.5
Or see this region's nucleotide sequence