Experiment: marine broth
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_2077 and B158DRAFT_2078 overlap by 4 nucleotides B158DRAFT_2078 and B158DRAFT_2079 are separated by 4 nucleotides B158DRAFT_2079 and B158DRAFT_2080 are separated by 26 nucleotides B158DRAFT_2080 and B158DRAFT_2081 are separated by 206 nucleotides B158DRAFT_2081 and B158DRAFT_2082 overlap by 4 nucleotides
B158DRAFT_2077: B158DRAFT_2077 - Predicted P-loop-containing kinase, at 217,415 to 218,266
_2077
B158DRAFT_2078: B158DRAFT_2078 - PTS IIA-like nitrogen-regulatory protein PtsN, at 218,263 to 218,811
_2078
B158DRAFT_2079: B158DRAFT_2079 - ribosomal subunit interface protein, at 218,816 to 219,103
_2079
B158DRAFT_2080: B158DRAFT_2080 - RNA polymerase, sigma 54 subunit, RpoN/SigL, at 219,130 to 220,602
_2080
B158DRAFT_2081: B158DRAFT_2081 - ABC-type (unclassified) transport system, ATPase component, at 220,809 to 221,534
_2081
B158DRAFT_2082: B158DRAFT_2082 - Uncharacterized protein conserved in bacteria, at 221,531 to 222,292
_2082
Position (kb) 219
220
221 Strain fitness (log2 ratio) -2
-1
0
1 at 218.131 kb on + strand, within B158DRAFT_2077 at 218.131 kb on + strand, within B158DRAFT_2077 at 218.139 kb on - strand, within B158DRAFT_2077 at 218.228 kb on + strand at 218.236 kb on - strand at 218.330 kb on - strand, within B158DRAFT_2078 at 218.340 kb on - strand, within B158DRAFT_2078 at 218.346 kb on - strand, within B158DRAFT_2078 at 218.347 kb on - strand, within B158DRAFT_2078 at 218.388 kb on + strand, within B158DRAFT_2078 at 218.406 kb on - strand, within B158DRAFT_2078 at 218.473 kb on - strand, within B158DRAFT_2078 at 218.475 kb on + strand, within B158DRAFT_2078 at 218.518 kb on + strand, within B158DRAFT_2078 at 218.518 kb on + strand, within B158DRAFT_2078 at 218.532 kb on + strand, within B158DRAFT_2078 at 218.532 kb on + strand, within B158DRAFT_2078 at 218.532 kb on + strand, within B158DRAFT_2078 at 218.573 kb on - strand, within B158DRAFT_2078 at 218.584 kb on + strand, within B158DRAFT_2078 at 218.586 kb on + strand, within B158DRAFT_2078 at 218.589 kb on + strand, within B158DRAFT_2078 at 218.589 kb on + strand, within B158DRAFT_2078 at 218.604 kb on - strand, within B158DRAFT_2078 at 218.604 kb on - strand, within B158DRAFT_2078 at 218.619 kb on - strand, within B158DRAFT_2078 at 218.653 kb on - strand, within B158DRAFT_2078 at 218.789 kb on - strand at 218.814 kb on + strand at 218.814 kb on + strand at 218.822 kb on - strand at 218.822 kb on - strand at 218.822 kb on - strand at 218.822 kb on - strand at 218.822 kb on - strand at 218.822 kb on - strand at 218.891 kb on + strand, within B158DRAFT_2079 at 218.938 kb on + strand, within B158DRAFT_2079 at 219.103 kb on - strand at 219.154 kb on - strand at 219.264 kb on - strand at 219.264 kb on - strand at 219.292 kb on - strand, within B158DRAFT_2080 at 219.587 kb on - strand, within B158DRAFT_2080 at 219.587 kb on - strand, within B158DRAFT_2080 at 219.587 kb on - strand, within B158DRAFT_2080 at 219.605 kb on - strand, within B158DRAFT_2080 at 219.640 kb on - strand, within B158DRAFT_2080 at 219.689 kb on + strand, within B158DRAFT_2080 at 219.697 kb on - strand, within B158DRAFT_2080 at 219.697 kb on - strand, within B158DRAFT_2080 at 219.739 kb on - strand, within B158DRAFT_2080 at 219.795 kb on - strand, within B158DRAFT_2080 at 219.797 kb on + strand, within B158DRAFT_2080 at 220.195 kb on - strand, within B158DRAFT_2080 at 220.212 kb on - strand, within B158DRAFT_2080 at 220.314 kb on - strand, within B158DRAFT_2080 at 220.317 kb on + strand, within B158DRAFT_2080 at 220.434 kb on + strand, within B158DRAFT_2080 at 220.434 kb on + strand, within B158DRAFT_2080 at 220.434 kb on + strand, within B158DRAFT_2080 at 220.434 kb on + strand, within B158DRAFT_2080 at 220.434 kb on + strand, within B158DRAFT_2080 at 220.442 kb on - strand, within B158DRAFT_2080 at 220.442 kb on - strand, within B158DRAFT_2080 at 220.510 kb on - strand at 220.542 kb on + strand at 220.661 kb on + strand at 220.765 kb on + strand at 220.773 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction marine broth remove 218,131 + B158DRAFT_2077 0.84 -1.5 218,131 + B158DRAFT_2077 0.84 +0.3 218,139 - B158DRAFT_2077 0.85 -2.0 218,228 + +0.4 218,236 - -1.5 218,330 - B158DRAFT_2078 0.12 -0.8 218,340 - B158DRAFT_2078 0.14 -0.5 218,346 - B158DRAFT_2078 0.15 -0.1 218,347 - B158DRAFT_2078 0.15 -0.9 218,388 + B158DRAFT_2078 0.23 -0.6 218,406 - B158DRAFT_2078 0.26 +0.1 218,473 - B158DRAFT_2078 0.38 -0.1 218,475 + B158DRAFT_2078 0.39 -1.1 218,518 + B158DRAFT_2078 0.46 -0.2 218,518 + B158DRAFT_2078 0.46 -0.7 218,532 + B158DRAFT_2078 0.49 -0.5 218,532 + B158DRAFT_2078 0.49 -0.5 218,532 + B158DRAFT_2078 0.49 -0.8 218,573 - B158DRAFT_2078 0.56 +0.0 218,584 + B158DRAFT_2078 0.58 -0.2 218,586 + B158DRAFT_2078 0.59 -0.0 218,589 + B158DRAFT_2078 0.59 -0.4 218,589 + B158DRAFT_2078 0.59 -1.7 218,604 - B158DRAFT_2078 0.62 +0.1 218,604 - B158DRAFT_2078 0.62 -0.2 218,619 - B158DRAFT_2078 0.65 -0.6 218,653 - B158DRAFT_2078 0.71 -0.0 218,789 - -0.2 218,814 + +0.5 218,814 + -0.0 218,822 - -1.2 218,822 - +0.6 218,822 - +0.5 218,822 - +0.7 218,822 - +0.3 218,822 - -0.7 218,891 + B158DRAFT_2079 0.26 +0.5 218,938 + B158DRAFT_2079 0.42 -0.1 219,103 - -0.5 219,154 - +0.5 219,264 - +0.0 219,264 - +0.0 219,292 - B158DRAFT_2080 0.11 -0.3 219,587 - B158DRAFT_2080 0.31 +0.7 219,587 - B158DRAFT_2080 0.31 -0.3 219,587 - B158DRAFT_2080 0.31 +0.0 219,605 - B158DRAFT_2080 0.32 -0.6 219,640 - B158DRAFT_2080 0.35 -0.4 219,689 + B158DRAFT_2080 0.38 +0.2 219,697 - B158DRAFT_2080 0.38 +0.3 219,697 - B158DRAFT_2080 0.38 -0.9 219,739 - B158DRAFT_2080 0.41 -0.1 219,795 - B158DRAFT_2080 0.45 -0.3 219,797 + B158DRAFT_2080 0.45 -0.6 220,195 - B158DRAFT_2080 0.72 +0.4 220,212 - B158DRAFT_2080 0.73 -1.1 220,314 - B158DRAFT_2080 0.80 +0.9 220,317 + B158DRAFT_2080 0.81 +0.8 220,434 + B158DRAFT_2080 0.89 -0.1 220,434 + B158DRAFT_2080 0.89 -0.2 220,434 + B158DRAFT_2080 0.89 -0.2 220,434 + B158DRAFT_2080 0.89 +0.8 220,434 + B158DRAFT_2080 0.89 -0.8 220,442 - B158DRAFT_2080 0.89 -0.3 220,442 - B158DRAFT_2080 0.89 +0.1 220,510 - -0.4 220,542 + -0.2 220,661 + -0.4 220,765 + +0.1 220,773 - -0.0
Or see this region's nucleotide sequence