Experiment: 6-methoxy-2(3H)-benzoxazolone 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02745 and PS417_02750 are separated by 80 nucleotides PS417_02750 and PS417_02755 overlap by 4 nucleotides PS417_02755 and PS417_02760 are separated by 97 nucleotides PS417_02760 and PS417_02765 are separated by 71 nucleotides
PS417_02745: PS417_02745 - FMN-dependent NADH-azoreductase, at 625,667 to 626,308
_02745
PS417_02750: PS417_02750 - alpha/beta hydrolase, at 626,389 to 627,279
_02750
PS417_02755: PS417_02755 - membrane protein, at 627,276 to 628,145
_02755
PS417_02760: PS417_02760 - AraC family transcriptional regulator, at 628,243 to 629,031
_02760
PS417_02765: PS417_02765 - phosphate starvation-inducible protein PsiF, at 629,103 to 629,405
_02765
Position (kb) 627
628
629 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 626.307 kb on - strand at 626.347 kb on + strand at 626.348 kb on - strand at 626.358 kb on + strand at 626.358 kb on + strand at 626.359 kb on - strand at 626.359 kb on - strand at 626.359 kb on - strand at 626.361 kb on + strand at 626.361 kb on + strand at 626.361 kb on + strand at 626.362 kb on - strand at 626.556 kb on - strand, within PS417_02750 at 626.556 kb on - strand, within PS417_02750 at 626.756 kb on - strand, within PS417_02750 at 626.923 kb on + strand, within PS417_02750 at 626.990 kb on - strand, within PS417_02750 at 627.127 kb on + strand, within PS417_02750 at 627.128 kb on - strand, within PS417_02750 at 627.163 kb on + strand, within PS417_02750 at 627.164 kb on - strand, within PS417_02750 at 627.349 kb on + strand at 627.503 kb on - strand, within PS417_02755 at 627.503 kb on - strand, within PS417_02755 at 627.598 kb on + strand, within PS417_02755 at 627.598 kb on + strand, within PS417_02755 at 627.652 kb on + strand at 627.793 kb on + strand, within PS417_02755 at 627.794 kb on - strand, within PS417_02755 at 627.901 kb on + strand, within PS417_02755 at 627.916 kb on + strand, within PS417_02755 at 627.916 kb on + strand, within PS417_02755 at 627.948 kb on + strand, within PS417_02755 at 627.949 kb on - strand, within PS417_02755 at 627.949 kb on - strand, within PS417_02755 at 627.949 kb on - strand, within PS417_02755 at 627.949 kb on - strand, within PS417_02755 at 628.014 kb on - strand, within PS417_02755 at 628.138 kb on + strand at 628.138 kb on + strand at 628.138 kb on + strand at 628.138 kb on + strand at 628.139 kb on - strand at 628.203 kb on - strand at 628.205 kb on - strand at 628.242 kb on + strand at 628.243 kb on - strand at 628.259 kb on + strand at 628.260 kb on - strand at 628.260 kb on - strand at 628.297 kb on + strand at 628.471 kb on + strand, within PS417_02760 at 628.471 kb on + strand, within PS417_02760 at 628.471 kb on + strand, within PS417_02760 at 628.471 kb on + strand, within PS417_02760 at 628.472 kb on - strand, within PS417_02760 at 628.472 kb on - strand, within PS417_02760 at 628.492 kb on + strand, within PS417_02760 at 628.492 kb on + strand, within PS417_02760 at 628.493 kb on - strand, within PS417_02760 at 629.037 kb on + strand at 629.038 kb on - strand at 629.051 kb on + strand at 629.051 kb on + strand at 629.051 kb on + strand at 629.052 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 6-methoxy-2(3H)-benzoxazolone 2.5 mM remove 626,307 - -0.1 626,347 + -2.7 626,348 - -2.1 626,358 + -0.3 626,358 + -1.5 626,359 - +1.7 626,359 - -1.5 626,359 - -0.4 626,361 + -2.7 626,361 + -1.1 626,361 + +0.6 626,362 - -0.2 626,556 - PS417_02750 0.19 -0.0 626,556 - PS417_02750 0.19 +0.0 626,756 - PS417_02750 0.41 -0.0 626,923 + PS417_02750 0.60 +0.1 626,990 - PS417_02750 0.67 +0.2 627,127 + PS417_02750 0.83 +0.1 627,128 - PS417_02750 0.83 -0.9 627,163 + PS417_02750 0.87 -0.4 627,164 - PS417_02750 0.87 -1.1 627,349 + -0.8 627,503 - PS417_02755 0.26 +0.1 627,503 - PS417_02755 0.26 -0.2 627,598 + PS417_02755 0.37 +0.1 627,598 + PS417_02755 0.37 -0.9 627,652 + +0.8 627,793 + PS417_02755 0.59 -0.2 627,794 - PS417_02755 0.60 -0.3 627,901 + PS417_02755 0.72 -3.2 627,916 + PS417_02755 0.74 -0.3 627,916 + PS417_02755 0.74 -0.8 627,948 + PS417_02755 0.77 -1.2 627,949 - PS417_02755 0.77 -0.6 627,949 - PS417_02755 0.77 -0.2 627,949 - PS417_02755 0.77 -0.2 627,949 - PS417_02755 0.77 +0.5 628,014 - PS417_02755 0.85 -1.5 628,138 + +0.5 628,138 + +0.5 628,138 + -0.2 628,138 + +0.7 628,139 - -0.6 628,203 - +0.9 628,205 - +0.3 628,242 + +0.7 628,243 - -0.3 628,259 + -0.7 628,260 - -1.5 628,260 - -0.2 628,297 + +0.8 628,471 + PS417_02760 0.29 +0.9 628,471 + PS417_02760 0.29 +0.2 628,471 + PS417_02760 0.29 +0.2 628,471 + PS417_02760 0.29 -0.7 628,472 - PS417_02760 0.29 -0.7 628,472 - PS417_02760 0.29 -1.9 628,492 + PS417_02760 0.32 -0.3 628,492 + PS417_02760 0.32 -0.7 628,493 - PS417_02760 0.32 -0.1 629,037 + +1.4 629,038 - +0.6 629,051 + +0.4 629,051 + -1.5 629,051 + -0.0 629,052 - +0.5
Or see this region's nucleotide sequence