Strain Fitness in Pseudomonas putida KT2440 around PP_t10

Experiment: 3-Hydroxyhexanoic (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0625 and PP_t07 are separated by 362 nucleotidesPP_t07 and PP_t08 are separated by 4 nucleotidesPP_t08 and PP_t09 are separated by 6 nucleotidesPP_t09 and PP_t10 are separated by 46 nucleotidesPP_t10 and PP_t11 are separated by 5 nucleotidesPP_t11 and PP_0626 are separated by 190 nucleotides PP_0625: PP_0625 - Chaperone protein ClpB, at 730,461 to 733,025 _0625 PP_t07: PP_t07 - tRNA-Asn, at 733,388 to 733,463 _t07 PP_t08: PP_t08 - tRNA-Pro, at 733,468 to 733,544 _t08 PP_t09: PP_t09 - tRNA-Lys, at 733,551 to 733,626 _t09 PP_t10: PP_t10 - tRNA-Pro, at 733,673 to 733,749 _t10 PP_t11: PP_t11 - tRNA-Lys, at 733,755 to 733,830 _t11 PP_0626: PP_0626 - NADH dehydrogenase, at 734,021 to 735,316 _0626 Position (kb) 733 734Strain fitness (log2 ratio) -2 -1 0 1 2at 732.777 kb on - strandat 732.889 kb on - strandat 733.179 kb on + strandat 733.181 kb on + strandat 733.181 kb on + strandat 733.181 kb on + strandat 733.475 kb on + strandat 733.475 kb on + strandat 733.629 kb on + strandat 733.681 kb on - strand, within PP_t10at 733.681 kb on - strand, within PP_t10at 733.763 kb on - strand, within PP_t11at 733.763 kb on - strand, within PP_t11at 733.775 kb on - strand, within PP_t11at 733.775 kb on - strand, within PP_t11at 733.775 kb on - strand, within PP_t11at 733.912 kb on + strandat 733.913 kb on - strandat 733.913 kb on - strandat 733.930 kb on + strandat 733.931 kb on - strandat 733.931 kb on - strandat 733.934 kb on + strandat 733.934 kb on + strandat 733.934 kb on + strandat 733.935 kb on - strandat 733.935 kb on - strandat 733.935 kb on - strandat 733.935 kb on - strandat 733.935 kb on - strandat 733.935 kb on - strandat 733.935 kb on - strandat 733.935 kb on - strandat 733.989 kb on - strandat 734.022 kb on + strandat 734.023 kb on - strandat 734.100 kb on + strandat 734.124 kb on + strandat 734.124 kb on + strandat 734.136 kb on + strandat 734.136 kb on + strandat 734.137 kb on - strandat 734.137 kb on - strandat 734.137 kb on - strandat 734.137 kb on - strandat 734.137 kb on - strandat 734.238 kb on + strand, within PP_0626at 734.238 kb on + strand, within PP_0626at 734.239 kb on - strand, within PP_0626at 734.239 kb on - strand, within PP_0626at 734.247 kb on + strand, within PP_0626at 734.248 kb on - strand, within PP_0626at 734.253 kb on + strand, within PP_0626at 734.254 kb on - strandat 734.254 kb on - strand, within PP_0626at 734.254 kb on - strand, within PP_0626at 734.254 kb on - strand, within PP_0626at 734.254 kb on - strand, within PP_0626at 734.347 kb on + strand, within PP_0626at 734.731 kb on - strand, within PP_0626at 734.731 kb on - strand, within PP_0626

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Per-strain Table

Position Strand Gene LocusTag Fraction 3-Hydroxyhexanoic (C)
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732,777 - -0.2
732,889 - +0.3
733,179 + +1.2
733,181 + +2.5
733,181 + -0.2
733,181 + -0.1
733,475 + +0.9
733,475 + +0.6
733,629 + -0.3
733,681 - PP_t10 0.10 -1.2
733,681 - PP_t10 0.10 -1.2
733,763 - PP_t11 0.11 -0.3
733,763 - PP_t11 0.11 +0.5
733,775 - PP_t11 0.26 +0.1
733,775 - PP_t11 0.26 +2.5
733,775 - PP_t11 0.26 -0.1
733,912 + +0.1
733,913 - +0.5
733,913 - +1.2
733,930 + +0.3
733,931 - +0.1
733,931 - +0.3
733,934 + +0.2
733,934 + -0.1
733,934 + -0.3
733,935 - -0.9
733,935 - +1.6
733,935 - +0.1
733,935 - +0.3
733,935 - -1.2
733,935 - +2.5
733,935 - -0.5
733,935 - -0.2
733,989 - +0.4
734,022 + +0.9
734,023 - +1.0
734,100 + +0.1
734,124 + -0.1
734,124 + -0.5
734,136 + -0.3
734,136 + +0.5
734,137 - +0.0
734,137 - -0.9
734,137 - +1.5
734,137 - -0.4
734,137 - -0.4
734,238 + PP_0626 0.17 +0.1
734,238 + PP_0626 0.17 +0.5
734,239 - PP_0626 0.17 +0.6
734,239 - PP_0626 0.17 -1.9
734,247 + PP_0626 0.17 -0.6
734,248 - PP_0626 0.18 +0.8
734,253 + PP_0626 0.18 +0.5
734,254 - -0.6
734,254 - PP_0626 0.18 -0.6
734,254 - PP_0626 0.18 -0.6
734,254 - PP_0626 0.18 -0.8
734,254 - PP_0626 0.18 -0.4
734,347 + PP_0626 0.25 +0.5
734,731 - PP_0626 0.55 -0.4
734,731 - PP_0626 0.55 -0.7

Or see this region's nucleotide sequence