Strain Fitness in Pseudomonas putida KT2440 around PP_2000

Experiment: 3-Hydroxyhexanoic (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_1998 and PP_1999 are separated by 196 nucleotidesPP_1999 and PP_2000 are separated by 42 nucleotidesPP_2000 and PP_2001 are separated by 16 nucleotides PP_1998: PP_1998 - putative cell division protein, at 2,266,235 to 2,266,879 _1998 PP_1999: PP_1999 - membrane protein required for colicin V production, at 2,267,076 to 2,267,633 _1999 PP_2000: PP_2000 - amidophosphoribosyl transferase, at 2,267,676 to 2,269,181 _2000 PP_2001: PP_2001 - O-succinylhomoserine sulfhydrylase, at 2,269,198 to 2,270,409 _2001 Position (kb) 2267 2268 2269 2270Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 2266.717 kb on + strand, within PP_1998at 2266.727 kb on + strand, within PP_1998at 2266.727 kb on + strand, within PP_1998at 2266.727 kb on + strand, within PP_1998at 2266.728 kb on - strand, within PP_1998at 2266.728 kb on - strand, within PP_1998at 2266.728 kb on - strandat 2266.736 kb on + strand, within PP_1998at 2266.736 kb on + strand, within PP_1998at 2266.736 kb on + strand, within PP_1998at 2266.736 kb on + strand, within PP_1998at 2266.737 kb on - strand, within PP_1998at 2266.737 kb on - strand, within PP_1998at 2266.737 kb on - strand, within PP_1998at 2266.738 kb on + strand, within PP_1998at 2266.769 kb on + strand, within PP_1998at 2266.770 kb on - strand, within PP_1998at 2266.931 kb on + strandat 2267.084 kb on + strandat 2267.084 kb on + strandat 2267.232 kb on + strand, within PP_1999at 2267.244 kb on + strand, within PP_1999at 2267.244 kb on + strand, within PP_1999at 2267.941 kb on - strand, within PP_2000at 2268.364 kb on - strand, within PP_2000at 2269.170 kb on + strandat 2269.333 kb on + strand, within PP_2001at 2269.333 kb on + strand, within PP_2001at 2269.334 kb on - strand, within PP_2001at 2269.406 kb on - strand, within PP_2001at 2269.477 kb on - strand, within PP_2001at 2269.477 kb on - strand, within PP_2001at 2269.591 kb on + strand, within PP_2001at 2269.591 kb on + strand, within PP_2001at 2269.591 kb on + strand, within PP_2001at 2269.592 kb on - strand, within PP_2001at 2269.592 kb on - strand, within PP_2001at 2269.592 kb on - strand, within PP_2001at 2269.592 kb on - strand, within PP_2001at 2269.610 kb on + strand, within PP_2001at 2269.611 kb on - strand, within PP_2001at 2269.611 kb on - strand, within PP_2001at 2269.621 kb on + strand, within PP_2001at 2269.725 kb on + strand, within PP_2001at 2269.726 kb on - strand, within PP_2001at 2269.903 kb on - strand, within PP_2001at 2270.010 kb on + strand, within PP_2001at 2270.011 kb on - strand, within PP_2001at 2270.011 kb on - strand, within PP_2001at 2270.086 kb on + strand, within PP_2001at 2270.087 kb on - strand, within PP_2001

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Per-strain Table

Position Strand Gene LocusTag Fraction 3-Hydroxyhexanoic (C)
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2,266,717 + PP_1998 0.75 -1.2
2,266,727 + PP_1998 0.76 +0.1
2,266,727 + PP_1998 0.76 +1.7
2,266,727 + PP_1998 0.76 -0.3
2,266,728 - PP_1998 0.76 -0.0
2,266,728 - PP_1998 0.76 +0.1
2,266,728 - +1.2
2,266,736 + PP_1998 0.78 +0.0
2,266,736 + PP_1998 0.78 -0.8
2,266,736 + PP_1998 0.78 -2.6
2,266,736 + PP_1998 0.78 -0.2
2,266,737 - PP_1998 0.78 +1.8
2,266,737 - PP_1998 0.78 -0.1
2,266,737 - PP_1998 0.78 +0.2
2,266,738 + PP_1998 0.78 -0.5
2,266,769 + PP_1998 0.83 +0.4
2,266,770 - PP_1998 0.83 -0.7
2,266,931 + -0.3
2,267,084 + -1.5
2,267,084 + -0.3
2,267,232 + PP_1999 0.28 -0.3
2,267,244 + PP_1999 0.30 -0.3
2,267,244 + PP_1999 0.30 +0.2
2,267,941 - PP_2000 0.18 -0.1
2,268,364 - PP_2000 0.46 +2.2
2,269,170 + +0.4
2,269,333 + PP_2001 0.11 -3.2
2,269,333 + PP_2001 0.11 -2.3
2,269,334 - PP_2001 0.11 -1.9
2,269,406 - PP_2001 0.17 -1.9
2,269,477 - PP_2001 0.23 -2.1
2,269,477 - PP_2001 0.23 -0.4
2,269,591 + PP_2001 0.32 -3.2
2,269,591 + PP_2001 0.32 -2.4
2,269,591 + PP_2001 0.32 -2.6
2,269,592 - PP_2001 0.33 -0.9
2,269,592 - PP_2001 0.33 -3.9
2,269,592 - PP_2001 0.33 -2.9
2,269,592 - PP_2001 0.33 -4.1
2,269,610 + PP_2001 0.34 -2.2
2,269,611 - PP_2001 0.34 -4.9
2,269,611 - PP_2001 0.34 -2.9
2,269,621 + PP_2001 0.35 -5.1
2,269,725 + PP_2001 0.43 -4.8
2,269,726 - PP_2001 0.44 -1.7
2,269,903 - PP_2001 0.58 -5.2
2,270,010 + PP_2001 0.67 -2.1
2,270,011 - PP_2001 0.67 -3.7
2,270,011 - PP_2001 0.67 -0.9
2,270,086 + PP_2001 0.73 -2.0
2,270,087 - PP_2001 0.73 -1.1

Or see this region's nucleotide sequence