Strain Fitness in Pseudomonas putida KT2440 around PP_1986

Experiment: 3-Hydroxyhexanoic (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_1985 and PP_1986 overlap by 4 nucleotidesPP_1986 and PP_1987 are separated by 118 nucleotidesPP_1987 and PP_1988 are separated by 54 nucleotides PP_1985: PP_1985 - 3-isopropylmalate dehydratase large subunit, at 2,250,670 to 2,252,103 _1985 PP_1986: PP_1986 - 3-isopropylmalate dehydratase small subunit, at 2,252,100 to 2,252,744 _1986 PP_1987: PP_1987 - Methlytransferase, UbiE/COQ5 family, at 2,252,863 to 2,253,627 _1987 PP_1988: PP_1988 - 3-isopropylmalate dehydrogenase, at 2,253,682 to 2,254,764 _1988 Position (kb) 2252 2253Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1 2at 2251.230 kb on + strand, within PP_1985at 2251.398 kb on + strand, within PP_1985at 2251.409 kb on - strand, within PP_1985at 2251.427 kb on - strand, within PP_1985at 2251.657 kb on + strand, within PP_1985at 2251.657 kb on + strand, within PP_1985at 2251.658 kb on - strand, within PP_1985at 2252.241 kb on + strand, within PP_1986at 2252.242 kb on - strand, within PP_1986at 2252.263 kb on - strand, within PP_1986at 2252.320 kb on - strand, within PP_1986at 2252.403 kb on + strand, within PP_1986at 2252.780 kb on - strandat 2252.784 kb on - strandat 2253.076 kb on + strand, within PP_1987at 2253.385 kb on + strand, within PP_1987at 2253.555 kb on - strandat 2253.562 kb on + strandat 2253.563 kb on - strandat 2253.563 kb on - strandat 2253.563 kb on - strandat 2253.565 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 3-Hydroxyhexanoic (C)
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2,251,230 + PP_1985 0.39 -2.8
2,251,398 + PP_1985 0.51 -2.7
2,251,409 - PP_1985 0.52 +1.2
2,251,427 - PP_1985 0.53 -0.8
2,251,657 + PP_1985 0.69 -2.7
2,251,657 + PP_1985 0.69 +2.2
2,251,658 - PP_1985 0.69 -6.1
2,252,241 + PP_1986 0.22 -1.1
2,252,242 - PP_1986 0.22 +2.2
2,252,263 - PP_1986 0.25 -1.2
2,252,320 - PP_1986 0.34 -3.1
2,252,403 + PP_1986 0.47 -4.3
2,252,780 - -0.7
2,252,784 - -0.3
2,253,076 + PP_1987 0.28 -0.6
2,253,385 + PP_1987 0.68 -1.5
2,253,555 - -1.6
2,253,562 + +0.2
2,253,563 - +2.2
2,253,563 - -0.3
2,253,563 - -2.9
2,253,565 + +0.6

Or see this region's nucleotide sequence