Experiment: Valerolactone (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4469 and PP_5693 overlap by 1 nucleotides PP_5693 and PP_4470 are separated by 69 nucleotides PP_4470 and PP_4471 are separated by 107 nucleotides PP_4471 and PP_t56 are separated by 396 nucleotides PP_t56 and PP_t55 are separated by 73 nucleotides PP_t55 and PP_t54 are separated by 74 nucleotides PP_t54 and PP_t53 are separated by 71 nucleotides
PP_4469: PP_4469 - ribose 1,5-bisphosphate phosphokinase, at 5,075,941 to 5,076,540
_4469
PP_5693: PP_5693 - conserved protein of unknown function, at 5,076,540 to 5,076,809
_5693
PP_4470: PP_4470 - putative transcriptional regulator (Arc domain-containing), at 5,076,879 to 5,077,205
_4470
PP_4471: PP_4471 - Magnesium transporter, at 5,077,313 to 5,078,755
_4471
PP_t56: PP_t56 - tRNA-Arg, at 5,079,152 to 5,079,228
_t56
PP_t55: PP_t55 - tRNA-Arg, at 5,079,302 to 5,079,378
_t55
PP_t54: PP_t54 - tRNA-Arg, at 5,079,453 to 5,079,529
_t54
PP_t53: PP_t53 - tRNA-Arg, at 5,079,601 to 5,079,677
_t53
Position (kb)
5077
5078
5079 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 5076.327 kb on + strand, within PP_4469 at 5076.488 kb on - strand at 5076.488 kb on - strand at 5076.534 kb on + strand at 5076.541 kb on + strand at 5076.541 kb on + strand at 5076.542 kb on - strand at 5076.542 kb on - strand at 5076.542 kb on - strand at 5076.548 kb on + strand at 5076.548 kb on + strand at 5076.548 kb on + strand at 5076.549 kb on - strand at 5076.549 kb on - strand at 5076.551 kb on - strand at 5076.636 kb on + strand, within PP_5693 at 5076.705 kb on - strand, within PP_5693 at 5076.716 kb on + strand, within PP_5693 at 5076.717 kb on - strand, within PP_5693 at 5076.727 kb on + strand, within PP_5693 at 5076.728 kb on - strand, within PP_5693 at 5076.728 kb on - strand, within PP_5693 at 5076.728 kb on - strand, within PP_5693 at 5076.728 kb on - strand, within PP_5693 at 5076.815 kb on + strand at 5076.816 kb on - strand at 5076.877 kb on - strand at 5076.877 kb on - strand at 5076.899 kb on + strand at 5076.903 kb on + strand at 5076.904 kb on - strand at 5076.974 kb on - strand, within PP_4470 at 5077.035 kb on - strand, within PP_4470 at 5077.035 kb on - strand, within PP_4470 at 5077.235 kb on + strand at 5077.235 kb on + strand at 5077.235 kb on + strand at 5077.236 kb on - strand at 5077.236 kb on - strand at 5077.236 kb on - strand at 5077.314 kb on + strand at 5077.314 kb on + strand at 5077.314 kb on + strand at 5077.315 kb on - strand at 5077.463 kb on + strand, within PP_4471 at 5077.518 kb on + strand, within PP_4471 at 5077.518 kb on + strand, within PP_4471 at 5077.518 kb on + strand at 5077.519 kb on - strand, within PP_4471 at 5077.519 kb on - strand, within PP_4471 at 5077.610 kb on - strand, within PP_4471 at 5077.984 kb on - strand, within PP_4471 at 5077.984 kb on - strand, within PP_4471 at 5077.984 kb on - strand, within PP_4471 at 5078.010 kb on + strand, within PP_4471 at 5078.011 kb on - strand, within PP_4471 at 5078.019 kb on - strand, within PP_4471 at 5078.108 kb on + strand, within PP_4471 at 5078.544 kb on + strand, within PP_4471 at 5078.545 kb on - strand, within PP_4471 at 5078.545 kb on - strand, within PP_4471 at 5078.545 kb on - strand, within PP_4471 at 5078.545 kb on - strand, within PP_4471 at 5078.744 kb on + strand at 5078.818 kb on - strand at 5078.818 kb on - strand at 5078.837 kb on - strand at 5078.840 kb on + strand at 5078.840 kb on + strand at 5078.840 kb on + strand at 5078.841 kb on - strand at 5078.841 kb on - strand at 5078.847 kb on + strand at 5078.847 kb on + strand at 5078.899 kb on + strand at 5078.899 kb on + strand at 5078.899 kb on + strand at 5078.899 kb on + strand at 5078.900 kb on - strand at 5078.900 kb on - strand at 5078.900 kb on - strand at 5078.900 kb on - strand at 5078.907 kb on + strand at 5078.907 kb on + strand at 5078.907 kb on + strand at 5078.908 kb on - strand at 5078.908 kb on - strand at 5078.908 kb on - strand at 5078.908 kb on - strand at 5078.981 kb on + strand at 5079.051 kb on - strand at 5079.051 kb on - strand at 5079.051 kb on - strand at 5079.051 kb on - strand at 5079.064 kb on + strand at 5079.076 kb on + strand at 5079.077 kb on - strand at 5079.077 kb on - strand at 5079.127 kb on - strand at 5079.127 kb on - strand at 5079.127 kb on - strand at 5079.151 kb on - strand at 5079.151 kb on - strand at 5079.207 kb on + strand, within PP_t56 at 5079.207 kb on + strand, within PP_t56 at 5079.237 kb on - strand at 5079.243 kb on - strand at 5079.243 kb on - strand at 5079.264 kb on - strand at 5079.370 kb on + strand, within PP_t55 at 5079.554 kb on - strand at 5079.695 kb on - strand at 5079.697 kb on - strand at 5079.697 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Valerolactone (C) remove 5,076,327 + PP_4469 0.64 +0.4 5,076,488 - -1.9 5,076,488 - +1.3 5,076,534 + -0.9 5,076,541 + -0.6 5,076,541 + -0.4 5,076,542 - -0.2 5,076,542 - +1.1 5,076,542 - +1.3 5,076,548 + +1.8 5,076,548 + +0.1 5,076,548 + -0.2 5,076,549 - -1.2 5,076,549 - +1.1 5,076,551 - +0.1 5,076,636 + PP_5693 0.36 -0.5 5,076,705 - PP_5693 0.61 -0.9 5,076,716 + PP_5693 0.65 +2.4 5,076,717 - PP_5693 0.66 -2.3 5,076,727 + PP_5693 0.69 -0.1 5,076,728 - PP_5693 0.70 -0.4 5,076,728 - PP_5693 0.70 -0.4 5,076,728 - PP_5693 0.70 -2.1 5,076,728 - PP_5693 0.70 -1.2 5,076,815 + +1.1 5,076,816 - -0.3 5,076,877 - -0.1 5,076,877 - -0.2 5,076,899 + -1.6 5,076,903 + +0.2 5,076,904 - -1.5 5,076,974 - PP_4470 0.29 -1.5 5,077,035 - PP_4470 0.48 -1.2 5,077,035 - PP_4470 0.48 -0.3 5,077,235 + +0.0 5,077,235 + -0.9 5,077,235 + +1.8 5,077,236 - +1.1 5,077,236 - -0.3 5,077,236 - -1.6 5,077,314 + -0.2 5,077,314 + +2.1 5,077,314 + +4.7 5,077,315 - -0.0 5,077,463 + PP_4471 0.10 +2.7 5,077,518 + PP_4471 0.14 +3.7 5,077,518 + PP_4471 0.14 -0.8 5,077,518 + +3.7 5,077,519 - PP_4471 0.14 +3.7 5,077,519 - PP_4471 0.14 +3.7 5,077,610 - PP_4471 0.21 +3.7 5,077,984 - PP_4471 0.47 +3.7 5,077,984 - PP_4471 0.47 +3.7 5,077,984 - PP_4471 0.47 +3.7 5,078,010 + PP_4471 0.48 -0.1 5,078,011 - PP_4471 0.48 +2.7 5,078,019 - PP_4471 0.49 +3.7 5,078,108 + PP_4471 0.55 +3.7 5,078,544 + PP_4471 0.85 +3.7 5,078,545 - PP_4471 0.85 +3.7 5,078,545 - PP_4471 0.85 -0.6 5,078,545 - PP_4471 0.85 -1.4 5,078,545 - PP_4471 0.85 +3.7 5,078,744 + +3.7 5,078,818 - -1.0 5,078,818 - -0.7 5,078,837 - -1.8 5,078,840 + +3.7 5,078,840 + +3.7 5,078,840 + +3.7 5,078,841 - -2.0 5,078,841 - -0.3 5,078,847 + +3.7 5,078,847 + +3.7 5,078,899 + +3.7 5,078,899 + +2.1 5,078,899 + +3.7 5,078,899 + +3.7 5,078,900 - -1.1 5,078,900 - -0.2 5,078,900 - -0.3 5,078,900 - -0.9 5,078,907 + +3.1 5,078,907 + +1.8 5,078,907 + +0.8 5,078,908 - -1.0 5,078,908 - -0.9 5,078,908 - +0.4 5,078,908 - -0.5 5,078,981 + +2.1 5,079,051 - -0.4 5,079,051 - +0.7 5,079,051 - -1.8 5,079,051 - +0.1 5,079,064 + +1.7 5,079,076 + +1.1 5,079,077 - -1.1 5,079,077 - -0.7 5,079,127 - +1.0 5,079,127 - -0.3 5,079,127 - -0.6 5,079,151 - -0.1 5,079,151 - +0.6 5,079,207 + PP_t56 0.71 +2.7 5,079,207 + PP_t56 0.71 +1.1 5,079,237 - +0.3 5,079,243 - +0.1 5,079,243 - -1.0 5,079,264 - +0.5 5,079,370 + PP_t55 0.88 +3.7 5,079,554 - +2.3 5,079,695 - +0.8 5,079,697 - +0.7 5,079,697 - +0.2
Or see this region's nucleotide sequence