Strain Fitness in Escherichia coli BW25113 around b4106

Experiment: LB_plus_SM_buffer with N4_phage 23.4375 MOI in droplets

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	LB_plus_SM_buffer with N4_phage 23.4375 MOI in droplets
4,321,421	-				-0.5
4,321,440	-				+0.0
4,321,440	-				-0.3
4,321,512	-	phnD	b4105	0.15	+0.1
4,321,572	+	phnD	b4105	0.21	-0.5
4,321,637	-	phnD	b4105	0.27	-0.3
4,321,637	-	phnD	b4105	0.27	+1.3
4,321,713	-	phnD	b4105	0.35	+0.4
4,321,713	-	phnD	b4105	0.35	-0.2
4,321,716	+	phnD	b4105	0.35	-0.2
4,321,834	+	phnD	b4105	0.47	-0.2
4,321,834	+	phnD	b4105	0.47	+0.6
4,321,915	-	phnD	b4105	0.55	-0.3
4,322,004	+	phnD	b4105	0.63	+0.2
4,322,088	-	phnD	b4105	0.72	-0.8
4,322,155	+	phnD	b4105	0.78	-0.0
4,322,155	+	phnD	b4105	0.78	-0.1
4,322,249	-	phnD	b4105	0.88	+0.3
4,322,310	+				-0.4
4,322,328	+				+0.0
4,322,335	-				+1.0
4,322,415	+				-0.3
4,322,415	+				-0.4
4,322,429	+				-0.2
4,322,463	+				+0.1
4,322,471	+				-0.1
4,322,471	+				-0.1
4,322,525	+	phnC	b4106	0.16	-0.4
4,322,538	-	phnC	b4106	0.17	+0.5
4,322,563	+	phnC	b4106	0.21	-0.5
4,322,563	+	phnC	b4106	0.21	+0.5
4,322,841	-	phnC	b4106	0.56	+0.7
4,322,856	-	phnC	b4106	0.58	+0.2
4,322,915	+	phnC	b4106	0.65	+1.5
4,322,915	+	phnC	b4106	0.65	-0.7
4,322,963	-	phnC	b4106	0.71	+0.2
4,322,988	-	phnC	b4106	0.75	-1.3
4,323,255	+				+0.1
4,323,352	+				-0.1
4,323,369	+	phnB	b4107	0.11	-0.2
4,323,369	+	phnB	b4107	0.11	-0.3
4,323,387	+	phnB	b4107	0.15	+0.3
4,323,500	+	phnB	b4107	0.40	+0.1
4,323,544	-	phnB	b4107	0.50	+0.6
4,323,556	-	phnB	b4107	0.53	-1.4
4,323,556	-	phnB	b4107	0.53	+0.5
4,323,568	+	phnB	b4107	0.56	-0.3
4,323,613	+	phnB	b4107	0.66	-0.2
4,323,686	-	phnB	b4107	0.82	-0.4
4,323,686	-	phnB	b4107	0.82	-0.4
4,323,686	-	phnB	b4107	0.82	-1.1
4,323,769	-				+0.1
4,323,769	-				-1.1
4,323,776	+				-0.2
4,323,826	-				+0.1
4,323,860	-				+0.2
4,324,091	+				+0.2
4,324,091	+				+0.5
4,324,101	+				+0.2