Experiment: LB_plus_SM_buffer with N4_phage 234.375 MOI in droplets
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybaZ and ffs are separated by 76 nucleotides ffs and ybaA are separated by 110 nucleotides ybaA and ylaB are separated by 41 nucleotides ylaB and ylaC are separated by 163 nucleotides ylaC and maa are separated by 115 nucleotides
b0454: ybaZ - predicted methyltransferase (NCBI), at 475,206 to 475,595
ybaZ
b0455: ffs - 4.5S RNA (NCBI), at 475,672 to 475,785
ffs
b0456: ybaA - hypothetical protein (NCBI), at 475,896 to 476,249
ybaA
b0457: ylaB - orf, hypothetical protein (VIMSS), at 476,291 to 477,841
ylaB
b0458: ylaC - orf, hypothetical protein (VIMSS), at 478,005 to 478,475
ylaC
b0459: maa - maltose O-acetyltransferase (NCBI), at 478,591 to 479,142
maa
Position (kb) 476
477
478 Strain fitness (log2 ratio) -2
-1
0
1
2 at 475.293 kb on + strand, within ybaZ at 475.293 kb on - strand, within ybaZ at 475.365 kb on - strand, within ybaZ at 475.413 kb on - strand, within ybaZ at 475.413 kb on - strand, within ybaZ at 475.436 kb on - strand, within ybaZ at 475.444 kb on - strand, within ybaZ at 475.444 kb on - strand, within ybaZ at 475.463 kb on - strand, within ybaZ at 475.483 kb on - strand, within ybaZ at 475.964 kb on + strand, within ybaA at 476.028 kb on - strand, within ybaA at 476.028 kb on - strand, within ybaA at 476.167 kb on + strand, within ybaA at 476.195 kb on + strand, within ybaA at 476.201 kb on - strand, within ybaA at 476.349 kb on + strand at 476.349 kb on + strand at 476.353 kb on + strand at 476.383 kb on - strand at 476.446 kb on - strand, within ylaB at 476.521 kb on - strand, within ylaB at 476.547 kb on - strand, within ylaB at 476.547 kb on - strand, within ylaB at 476.573 kb on - strand, within ylaB at 476.594 kb on + strand, within ylaB at 476.662 kb on + strand, within ylaB at 476.662 kb on + strand, within ylaB at 476.662 kb on - strand, within ylaB at 476.662 kb on - strand, within ylaB at 476.670 kb on - strand, within ylaB at 476.677 kb on + strand, within ylaB at 476.685 kb on - strand, within ylaB at 476.685 kb on - strand, within ylaB at 476.690 kb on - strand, within ylaB at 476.690 kb on - strand, within ylaB at 476.696 kb on - strand, within ylaB at 476.752 kb on + strand, within ylaB at 476.752 kb on + strand at 476.774 kb on + strand, within ylaB at 476.775 kb on - strand, within ylaB at 476.801 kb on + strand, within ylaB at 476.806 kb on - strand, within ylaB at 476.806 kb on - strand, within ylaB at 476.839 kb on - strand, within ylaB at 476.839 kb on - strand, within ylaB at 476.862 kb on - strand, within ylaB at 476.904 kb on - strand, within ylaB at 476.910 kb on + strand, within ylaB at 476.910 kb on + strand, within ylaB at 476.911 kb on + strand, within ylaB at 476.981 kb on - strand, within ylaB at 477.034 kb on + strand, within ylaB at 477.034 kb on + strand, within ylaB at 477.056 kb on - strand, within ylaB at 477.067 kb on - strand, within ylaB at 477.067 kb on - strand, within ylaB at 477.069 kb on + strand, within ylaB at 477.069 kb on + strand, within ylaB at 477.089 kb on - strand, within ylaB at 477.089 kb on - strand, within ylaB at 477.089 kb on - strand, within ylaB at 477.161 kb on - strand, within ylaB at 477.161 kb on - strand, within ylaB at 477.188 kb on + strand, within ylaB at 477.196 kb on - strand, within ylaB at 477.196 kb on - strand, within ylaB at 477.196 kb on - strand, within ylaB at 477.250 kb on + strand, within ylaB at 477.259 kb on - strand, within ylaB at 477.284 kb on + strand, within ylaB at 477.284 kb on + strand, within ylaB at 477.292 kb on - strand, within ylaB at 477.296 kb on + strand, within ylaB at 477.296 kb on + strand, within ylaB at 477.315 kb on - strand, within ylaB at 477.316 kb on - strand, within ylaB at 477.316 kb on - strand, within ylaB at 477.344 kb on - strand, within ylaB at 477.344 kb on - strand, within ylaB at 477.344 kb on - strand, within ylaB at 477.346 kb on - strand, within ylaB at 477.346 kb on - strand, within ylaB at 477.369 kb on + strand, within ylaB at 477.397 kb on + strand, within ylaB at 477.427 kb on - strand, within ylaB at 477.427 kb on - strand, within ylaB at 477.448 kb on + strand, within ylaB at 477.460 kb on - strand, within ylaB at 477.484 kb on + strand, within ylaB at 477.495 kb on + strand, within ylaB at 477.495 kb on + strand, within ylaB at 477.509 kb on + strand at 477.515 kb on - strand, within ylaB at 477.515 kb on - strand, within ylaB at 477.517 kb on - strand, within ylaB at 477.523 kb on - strand, within ylaB at 477.523 kb on - strand, within ylaB at 477.615 kb on + strand, within ylaB at 477.623 kb on - strand, within ylaB at 477.648 kb on - strand, within ylaB at 477.661 kb on - strand, within ylaB at 477.678 kb on + strand, within ylaB at 477.688 kb on + strand at 477.722 kb on - strand at 477.738 kb on + strand at 477.792 kb on + strand at 477.814 kb on - strand at 477.861 kb on - strand at 477.861 kb on - strand at 477.863 kb on + strand at 477.863 kb on + strand at 477.863 kb on + strand at 477.871 kb on - strand at 477.871 kb on - strand at 477.871 kb on - strand at 477.880 kb on - strand at 477.923 kb on + strand at 477.923 kb on + strand at 477.943 kb on + strand at 477.998 kb on - strand at 478.086 kb on - strand, within ylaC at 478.086 kb on - strand, within ylaC at 478.109 kb on + strand, within ylaC at 478.137 kb on - strand, within ylaC at 478.147 kb on + strand, within ylaC at 478.147 kb on - strand, within ylaC at 478.147 kb on - strand, within ylaC at 478.156 kb on - strand, within ylaC at 478.162 kb on + strand, within ylaC at 478.219 kb on - strand, within ylaC at 478.226 kb on - strand, within ylaC at 478.266 kb on + strand, within ylaC at 478.378 kb on - strand, within ylaC at 478.450 kb on + strand at 478.587 kb on - strand at 478.629 kb on + strand at 478.639 kb on + strand at 478.639 kb on + strand at 478.649 kb on + strand, within maa at 478.684 kb on + strand, within maa at 478.705 kb on + strand, within maa at 478.706 kb on + strand, within maa at 478.708 kb on + strand, within maa at 478.708 kb on + strand, within maa at 478.730 kb on + strand, within maa at 478.738 kb on - strand, within maa at 478.777 kb on + strand, within maa at 478.777 kb on + strand, within maa at 478.778 kb on + strand, within maa at 478.778 kb on + strand, within maa at 478.783 kb on - strand, within maa at 478.785 kb on + strand, within maa at 478.785 kb on - strand, within maa at 478.788 kb on + strand, within maa at 478.793 kb on - strand, within maa at 478.804 kb on - strand, within maa at 478.814 kb on - strand, within maa at 478.827 kb on - strand, within maa at 478.840 kb on + strand, within maa at 478.840 kb on + strand, within maa
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with N4_phage 234.375 MOI in droplets remove 475,293 + ybaZ b0454 0.22 +0.5 475,293 - ybaZ b0454 0.22 -0.6 475,365 - ybaZ b0454 0.41 +0.3 475,413 - ybaZ b0454 0.53 +0.6 475,413 - ybaZ b0454 0.53 +0.2 475,436 - ybaZ b0454 0.59 +0.4 475,444 - ybaZ b0454 0.61 -0.1 475,444 - ybaZ b0454 0.61 -0.6 475,463 - ybaZ b0454 0.66 -0.2 475,483 - ybaZ b0454 0.71 -0.0 475,964 + ybaA b0456 0.19 -0.0 476,028 - ybaA b0456 0.37 -0.3 476,028 - ybaA b0456 0.37 -0.3 476,167 + ybaA b0456 0.77 -0.4 476,195 + ybaA b0456 0.84 +0.5 476,201 - ybaA b0456 0.86 -0.0 476,349 + +0.7 476,349 + -0.1 476,353 + -0.0 476,383 - -0.8 476,446 - ylaB b0457 0.10 +0.4 476,521 - ylaB b0457 0.15 -0.7 476,547 - ylaB b0457 0.17 -0.9 476,547 - ylaB b0457 0.17 -0.2 476,573 - ylaB b0457 0.18 -0.3 476,594 + ylaB b0457 0.20 -0.4 476,662 + ylaB b0457 0.24 -0.7 476,662 + ylaB b0457 0.24 -1.3 476,662 - ylaB b0457 0.24 +0.2 476,662 - ylaB b0457 0.24 +1.1 476,670 - ylaB b0457 0.24 +0.5 476,677 + ylaB b0457 0.25 -0.6 476,685 - ylaB b0457 0.25 +0.3 476,685 - ylaB b0457 0.25 +0.8 476,690 - ylaB b0457 0.26 -0.5 476,690 - ylaB b0457 0.26 +0.1 476,696 - ylaB b0457 0.26 -1.4 476,752 + ylaB b0457 0.30 -0.6 476,752 + -1.5 476,774 + ylaB b0457 0.31 -1.4 476,775 - ylaB b0457 0.31 -1.5 476,801 + ylaB b0457 0.33 +0.0 476,806 - ylaB b0457 0.33 -0.0 476,806 - ylaB b0457 0.33 -0.6 476,839 - ylaB b0457 0.35 -0.3 476,839 - ylaB b0457 0.35 -0.7 476,862 - ylaB b0457 0.37 -0.2 476,904 - ylaB b0457 0.40 +0.3 476,910 + ylaB b0457 0.40 -0.1 476,910 + ylaB b0457 0.40 -0.5 476,911 + ylaB b0457 0.40 -0.1 476,981 - ylaB b0457 0.44 -0.3 477,034 + ylaB b0457 0.48 -0.9 477,034 + ylaB b0457 0.48 -0.3 477,056 - ylaB b0457 0.49 +0.1 477,067 - ylaB b0457 0.50 +0.3 477,067 - ylaB b0457 0.50 -0.2 477,069 + ylaB b0457 0.50 -0.6 477,069 + ylaB b0457 0.50 -0.0 477,089 - ylaB b0457 0.51 -0.3 477,089 - ylaB b0457 0.51 -0.6 477,089 - ylaB b0457 0.51 -0.5 477,161 - ylaB b0457 0.56 -0.5 477,161 - ylaB b0457 0.56 -0.6 477,188 + ylaB b0457 0.58 -0.2 477,196 - ylaB b0457 0.58 -0.7 477,196 - ylaB b0457 0.58 -0.2 477,196 - ylaB b0457 0.58 +0.7 477,250 + ylaB b0457 0.62 -0.1 477,259 - ylaB b0457 0.62 +0.1 477,284 + ylaB b0457 0.64 -0.8 477,284 + ylaB b0457 0.64 -0.9 477,292 - ylaB b0457 0.65 -0.4 477,296 + ylaB b0457 0.65 +0.2 477,296 + ylaB b0457 0.65 -2.0 477,315 - ylaB b0457 0.66 -0.6 477,316 - ylaB b0457 0.66 -1.2 477,316 - ylaB b0457 0.66 -0.5 477,344 - ylaB b0457 0.68 -0.7 477,344 - ylaB b0457 0.68 -0.9 477,344 - ylaB b0457 0.68 +0.1 477,346 - ylaB b0457 0.68 -1.5 477,346 - ylaB b0457 0.68 -1.8 477,369 + ylaB b0457 0.70 -0.8 477,397 + ylaB b0457 0.71 -0.7 477,427 - ylaB b0457 0.73 -0.3 477,427 - ylaB b0457 0.73 +0.1 477,448 + ylaB b0457 0.75 -0.2 477,460 - ylaB b0457 0.75 -1.4 477,484 + ylaB b0457 0.77 +0.2 477,495 + ylaB b0457 0.78 -1.5 477,495 + ylaB b0457 0.78 +0.7 477,509 + -1.1 477,515 - ylaB b0457 0.79 -0.7 477,515 - ylaB b0457 0.79 -0.2 477,517 - ylaB b0457 0.79 -0.1 477,523 - ylaB b0457 0.79 -0.8 477,523 - ylaB b0457 0.79 -2.1 477,615 + ylaB b0457 0.85 -0.9 477,623 - ylaB b0457 0.86 -0.8 477,648 - ylaB b0457 0.87 -1.8 477,661 - ylaB b0457 0.88 -0.2 477,678 + ylaB b0457 0.89 -0.6 477,688 + -0.6 477,722 - -0.7 477,738 + -0.9 477,792 + -0.5 477,814 - -0.1 477,861 - +0.7 477,861 - +0.6 477,863 + -1.0 477,863 + +0.2 477,863 + -0.3 477,871 - -0.1 477,871 - -0.7 477,871 - +0.4 477,880 - +0.4 477,923 + +0.1 477,923 + +0.3 477,943 + -0.3 477,998 - +0.5 478,086 - ylaC b0458 0.17 +0.1 478,086 - ylaC b0458 0.17 -0.9 478,109 + ylaC b0458 0.22 +2.4 478,137 - ylaC b0458 0.28 +0.0 478,147 + ylaC b0458 0.30 +0.2 478,147 - ylaC b0458 0.30 +0.6 478,147 - ylaC b0458 0.30 +0.3 478,156 - ylaC b0458 0.32 -0.5 478,162 + ylaC b0458 0.33 -0.2 478,219 - ylaC b0458 0.45 +0.1 478,226 - ylaC b0458 0.47 +0.5 478,266 + ylaC b0458 0.55 -0.8 478,378 - ylaC b0458 0.79 -0.2 478,450 + -0.1 478,587 - -0.1 478,629 + +0.0 478,639 + -0.2 478,639 + -1.4 478,649 + maa b0459 0.11 +0.1 478,684 + maa b0459 0.17 -0.6 478,705 + maa b0459 0.21 -0.8 478,706 + maa b0459 0.21 -0.0 478,708 + maa b0459 0.21 +1.9 478,708 + maa b0459 0.21 -0.8 478,730 + maa b0459 0.25 -0.1 478,738 - maa b0459 0.27 +0.3 478,777 + maa b0459 0.34 -0.6 478,777 + maa b0459 0.34 +1.0 478,778 + maa b0459 0.34 +0.5 478,778 + maa b0459 0.34 +0.4 478,783 - maa b0459 0.35 -1.3 478,785 + maa b0459 0.35 +0.1 478,785 - maa b0459 0.35 +1.3 478,788 + maa b0459 0.36 -0.4 478,793 - maa b0459 0.37 -0.2 478,804 - maa b0459 0.39 -0.1 478,814 - maa b0459 0.40 -0.2 478,827 - maa b0459 0.43 -0.9 478,840 + maa b0459 0.45 -0.0 478,840 + maa b0459 0.45 +0.5
Or see this region's nucleotide sequence