Experiment: Butyrolactam (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4320 and PP_5748 overlap by 8 nucleotides PP_5748 and PP_4321 overlap by 4 nucleotides PP_4321 and PP_4322 overlap by 4 nucleotides
PP_4320: PP_4320 - cytochrome c-type biogenesis protein, at 4,910,515 to 4,911,711
_4320
PP_5748: PP_5748 - cytochrome c heme lyase subunit, at 4,911,704 to 4,912,180
_5748
PP_4321: PP_4321 - holocytochrome c synthetase subunit, at 4,912,177 to 4,912,713
_4321
PP_4322: PP_4322 - holocytochrome c synthetase, at 4,912,710 to 4,914,698
_4322
Position (kb)
4911
4912
4913 Strain fitness (log2 ratio)
-1
0
1 at 4910.781 kb on + strand, within PP_4320 at 4910.782 kb on - strand, within PP_4320 at 4910.919 kb on + strand, within PP_4320 at 4910.984 kb on + strand, within PP_4320 at 4911.003 kb on - strand, within PP_4320 at 4911.198 kb on + strand, within PP_4320 at 4911.233 kb on + strand, within PP_4320 at 4911.234 kb on - strand, within PP_4320 at 4911.251 kb on + strand, within PP_4320 at 4911.251 kb on + strand, within PP_4320 at 4911.252 kb on - strand, within PP_4320 at 4911.252 kb on - strand, within PP_4320 at 4911.303 kb on + strand, within PP_4320 at 4911.384 kb on - strand at 4912.108 kb on - strand, within PP_5748 at 4912.108 kb on - strand, within PP_5748 at 4912.314 kb on - strand at 4912.854 kb on - strand at 4912.858 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Butyrolactam (N) remove 4,910,781 + PP_4320 0.22 +0.1 4,910,782 - PP_4320 0.22 +0.2 4,910,919 + PP_4320 0.34 +0.1 4,910,984 + PP_4320 0.39 -0.4 4,911,003 - PP_4320 0.41 +0.3 4,911,198 + PP_4320 0.57 +0.5 4,911,233 + PP_4320 0.60 -0.1 4,911,234 - PP_4320 0.60 +0.5 4,911,251 + PP_4320 0.61 -0.7 4,911,251 + PP_4320 0.61 -0.4 4,911,252 - PP_4320 0.62 -0.3 4,911,252 - PP_4320 0.62 +1.1 4,911,303 + PP_4320 0.66 -0.4 4,911,384 - +1.5 4,912,108 - PP_5748 0.85 -1.1 4,912,108 - PP_5748 0.85 -0.6 4,912,314 - +0.2 4,912,854 - -0.1 4,912,858 - +1.5
Or see this region's nucleotide sequence