Experiment: Butyrolactam (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5583 and PP_3110 overlap by 41 nucleotides PP_3110 and PP_5584 are separated by 8 nucleotides PP_5584 and PP_3113 are separated by 696 nucleotides
PP_5583: PP_5583 - conserved protein of unknown function, at 3,521,488 to 3,521,847
_5583
PP_3110: PP_3110 - protein of unknown function, at 3,521,807 to 3,522,019
_3110
PP_5584: PP_5584 - protein of unknown function, at 3,522,028 to 3,522,213
_5584
PP_3113: PP_3113 - transposase, at 3,522,910 to 3,523,269
_3113
Position (kb)
3521
3522
3523 Strain fitness (log2 ratio)
-1
0
1 at 3520.829 kb on + strand at 3520.830 kb on - strand at 3520.982 kb on + strand at 3520.983 kb on - strand at 3520.983 kb on - strand at 3521.051 kb on + strand at 3521.133 kb on + strand at 3521.140 kb on - strand at 3521.369 kb on + strand at 3521.370 kb on - strand at 3521.671 kb on - strand, within PP_5583 at 3521.799 kb on + strand, within PP_5583 at 3521.931 kb on - strand, within PP_3110 at 3521.987 kb on + strand, within PP_3110 at 3521.988 kb on - strand, within PP_3110 at 3522.284 kb on + strand at 3522.284 kb on + strand at 3522.284 kb on + strand at 3522.345 kb on - strand at 3522.428 kb on + strand at 3522.428 kb on + strand at 3522.428 kb on + strand at 3522.537 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Butyrolactam (N) remove 3,520,829 + -0.3 3,520,830 - -0.6 3,520,982 + -0.1 3,520,983 - +0.0 3,520,983 - -0.1 3,521,051 + -0.3 3,521,133 + +0.3 3,521,140 - +1.5 3,521,369 + +0.3 3,521,370 - -0.7 3,521,671 - PP_5583 0.51 -0.5 3,521,799 + PP_5583 0.86 -0.0 3,521,931 - PP_3110 0.58 -0.8 3,521,987 + PP_3110 0.85 +1.0 3,521,988 - PP_3110 0.85 -0.3 3,522,284 + +0.3 3,522,284 + +0.1 3,522,284 + -0.3 3,522,345 - -0.1 3,522,428 + -1.6 3,522,428 + +0.2 3,522,428 + +0.0 3,522,537 - +0.1
Or see this region's nucleotide sequence