Experiment: Nicotinic Acid (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3945 and PP_3946 are separated by 70 nucleotides PP_3946 and PP_3947 are separated by 441 nucleotides PP_3947 and PP_3948 overlap by 4 nucleotides
PP_3945: PP_3945 - 2,5-dihydroxypyridine 5,6-dioxygenase, at 4,447,886 to 4,448,938
_3945
PP_3946: PP_3946 - HTH-type transcriptional repressor NicR, at 4,449,009 to 4,449,485
_3946
PP_3947: PP_3947 - nicotinate dehydrogenase subunit A, at 4,449,927 to 4,450,400
_3947
PP_3948: PP_3948 - nicotinate dehydrogenase subunit B (EC 1.17.2.1) (from data) , at 4,450,397 to 4,453,960
_3948
Position (kb)
4449
4450
4451 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4448.943 kb on + strand at 4448.944 kb on - strand at 4448.944 kb on - strand at 4448.945 kb on + strand at 4448.945 kb on + strand at 4448.945 kb on + strand at 4448.946 kb on - strand at 4448.946 kb on - strand at 4448.946 kb on - strand at 4448.946 kb on - strand at 4448.946 kb on - strand at 4448.946 kb on - strand at 4449.008 kb on + strand at 4449.008 kb on + strand at 4449.009 kb on - strand at 4449.009 kb on - strand at 4449.009 kb on - strand at 4449.009 kb on - strand at 4449.150 kb on - strand, within PP_3946 at 4449.150 kb on - strand, within PP_3946 at 4449.441 kb on + strand at 4449.680 kb on - strand at 4449.680 kb on - strand at 4449.689 kb on + strand at 4449.690 kb on - strand at 4449.701 kb on + strand at 4449.800 kb on + strand at 4449.800 kb on + strand at 4449.801 kb on - strand at 4449.801 kb on - strand at 4449.801 kb on - strand at 4449.801 kb on - strand at 4449.801 kb on - strand at 4449.801 kb on - strand at 4449.801 kb on - strand at 4449.801 kb on - strand at 4449.801 kb on - strand at 4449.851 kb on + strand at 4449.858 kb on + strand at 4449.858 kb on + strand at 4449.858 kb on + strand at 4449.858 kb on + strand at 4449.859 kb on - strand at 4449.859 kb on - strand at 4449.925 kb on + strand at 4449.926 kb on - strand at 4449.926 kb on - strand at 4449.926 kb on - strand at 4449.926 kb on - strand at 4450.073 kb on + strand, within PP_3947 at 4450.073 kb on + strand, within PP_3947 at 4450.074 kb on - strand, within PP_3947 at 4450.074 kb on - strand, within PP_3947 at 4450.142 kb on + strand, within PP_3947 at 4450.142 kb on + strand, within PP_3947 at 4450.143 kb on - strand, within PP_3947 at 4450.231 kb on - strand, within PP_3947 at 4450.231 kb on - strand, within PP_3947 at 4450.231 kb on - strand, within PP_3947 at 4450.513 kb on + strand at 4450.513 kb on + strand at 4450.513 kb on + strand at 4450.513 kb on + strand at 4450.514 kb on - strand at 4450.571 kb on + strand at 4450.572 kb on - strand at 4450.613 kb on - strand at 4450.613 kb on - strand at 4450.782 kb on - strand, within PP_3948 at 4450.782 kb on - strand, within PP_3948 at 4450.782 kb on - strand, within PP_3948 at 4450.956 kb on - strand, within PP_3948 at 4450.986 kb on + strand, within PP_3948 at 4450.986 kb on + strand, within PP_3948 at 4450.986 kb on + strand, within PP_3948 at 4451.021 kb on + strand, within PP_3948 at 4451.021 kb on + strand, within PP_3948 at 4451.022 kb on - strand, within PP_3948 at 4451.022 kb on - strand, within PP_3948 at 4451.079 kb on - strand, within PP_3948 at 4451.284 kb on + strand, within PP_3948 at 4451.285 kb on - strand, within PP_3948 at 4451.396 kb on + strand, within PP_3948 at 4451.397 kb on - strand, within PP_3948
Per-strain Table
Position Strand Gene LocusTag Fraction Nicotinic Acid (N) remove 4,448,943 + -2.0 4,448,944 - +0.9 4,448,944 - +0.2 4,448,945 + +0.6 4,448,945 + -1.2 4,448,945 + -0.1 4,448,946 - +0.8 4,448,946 - -0.4 4,448,946 - -0.4 4,448,946 - -0.6 4,448,946 - +0.4 4,448,946 - -1.1 4,449,008 + -0.5 4,449,008 + -0.5 4,449,009 - -0.3 4,449,009 - -0.7 4,449,009 - +1.9 4,449,009 - -0.1 4,449,150 - PP_3946 0.30 +0.1 4,449,150 - PP_3946 0.30 -0.3 4,449,441 + +0.3 4,449,680 - -0.0 4,449,680 - +0.1 4,449,689 + -0.4 4,449,690 - -0.8 4,449,701 + -0.1 4,449,800 + +0.0 4,449,800 + +0.0 4,449,801 - -1.3 4,449,801 - +0.3 4,449,801 - -0.7 4,449,801 - -0.5 4,449,801 - -1.1 4,449,801 - +0.3 4,449,801 - -1.0 4,449,801 - -0.3 4,449,801 - -0.7 4,449,851 + -1.2 4,449,858 + +0.2 4,449,858 + +0.3 4,449,858 + -0.6 4,449,858 + +1.4 4,449,859 - -2.6 4,449,859 - -0.8 4,449,925 + -0.9 4,449,926 - -1.3 4,449,926 - -1.2 4,449,926 - -0.8 4,449,926 - -0.5 4,450,073 + PP_3947 0.31 -2.0 4,450,073 + PP_3947 0.31 -1.3 4,450,074 - PP_3947 0.31 -0.8 4,450,074 - PP_3947 0.31 -1.6 4,450,142 + PP_3947 0.45 -2.0 4,450,142 + PP_3947 0.45 -0.6 4,450,143 - PP_3947 0.46 -1.8 4,450,231 - PP_3947 0.64 -1.2 4,450,231 - PP_3947 0.64 -1.0 4,450,231 - PP_3947 0.64 -1.3 4,450,513 + -1.5 4,450,513 + -1.4 4,450,513 + -1.5 4,450,513 + -0.7 4,450,514 - -1.9 4,450,571 + -0.6 4,450,572 - -1.7 4,450,613 - -0.1 4,450,613 - -1.6 4,450,782 - PP_3948 0.11 -0.7 4,450,782 - PP_3948 0.11 -1.2 4,450,782 - PP_3948 0.11 -1.9 4,450,956 - PP_3948 0.16 -1.3 4,450,986 + PP_3948 0.17 -1.8 4,450,986 + PP_3948 0.17 -1.5 4,450,986 + PP_3948 0.17 -3.2 4,451,021 + PP_3948 0.18 -1.2 4,451,021 + PP_3948 0.18 -2.3 4,451,022 - PP_3948 0.18 -1.1 4,451,022 - PP_3948 0.18 -0.9 4,451,079 - PP_3948 0.19 -1.9 4,451,284 + PP_3948 0.25 -1.7 4,451,285 - PP_3948 0.25 -1.8 4,451,396 + PP_3948 0.28 -1.0 4,451,397 - PP_3948 0.28 -0.9
Or see this region's nucleotide sequence