Experiment: Imidazole (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0220 and PP_0221 are separated by 1 nucleotides PP_0221 and PP_0222 overlap by 8 nucleotides
PP_0220: PP_0220 - methionine import ATP-binding protein metN2, at 272,658 to 273,767
_0220
PP_0221: PP_0221 - methionine ABC transporter periplasmic-binding lipoprotein (metQ-like protein), at 273,769 to 274,569
_0221
PP_0222: PP_0222 - Monooxygenase, DszA family, at 274,562 to 275,947
_0222
Position (kb) 273
274
275 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 273.271 kb on + strand, within PP_0220 at 273.495 kb on + strand, within PP_0220 at 273.551 kb on + strand, within PP_0220 at 273.552 kb on - strand at 273.552 kb on - strand, within PP_0220 at 273.644 kb on - strand, within PP_0220 at 273.791 kb on + strand at 273.792 kb on - strand at 273.792 kb on - strand at 273.792 kb on - strand at 273.792 kb on - strand at 273.845 kb on + strand at 273.845 kb on + strand at 273.845 kb on + strand at 273.845 kb on + strand at 273.845 kb on + strand at 273.846 kb on - strand at 273.846 kb on - strand at 273.846 kb on - strand at 273.846 kb on - strand at 273.846 kb on - strand at 273.846 kb on - strand at 273.914 kb on + strand, within PP_0221 at 273.914 kb on + strand, within PP_0221 at 273.914 kb on + strand, within PP_0221 at 273.914 kb on + strand, within PP_0221 at 273.914 kb on + strand, within PP_0221 at 273.915 kb on - strand, within PP_0221 at 273.915 kb on - strand, within PP_0221 at 273.954 kb on + strand, within PP_0221 at 273.983 kb on + strand, within PP_0221 at 273.983 kb on + strand, within PP_0221 at 273.983 kb on + strand, within PP_0221 at 273.984 kb on - strand, within PP_0221 at 274.103 kb on + strand, within PP_0221 at 274.104 kb on - strand, within PP_0221 at 274.337 kb on + strand, within PP_0221 at 274.337 kb on + strand, within PP_0221 at 274.476 kb on + strand, within PP_0221 at 274.597 kb on + strand at 274.684 kb on - strand at 274.696 kb on - strand at 274.853 kb on + strand, within PP_0222 at 274.853 kb on + strand, within PP_0222 at 274.853 kb on + strand, within PP_0222 at 274.854 kb on - strand, within PP_0222 at 274.857 kb on + strand, within PP_0222 at 275.052 kb on + strand, within PP_0222 at 275.317 kb on - strand, within PP_0222 at 275.331 kb on + strand, within PP_0222 at 275.331 kb on + strand, within PP_0222 at 275.379 kb on + strand, within PP_0222 at 275.379 kb on + strand, within PP_0222 at 275.380 kb on - strand, within PP_0222 at 275.380 kb on - strand, within PP_0222 at 275.380 kb on - strand, within PP_0222 at 275.380 kb on - strand, within PP_0222 at 275.442 kb on + strand, within PP_0222 at 275.442 kb on + strand, within PP_0222 at 275.442 kb on + strand, within PP_0222 at 275.443 kb on - strand, within PP_0222 at 275.457 kb on + strand, within PP_0222
Per-strain Table
Position Strand Gene LocusTag Fraction Imidazole (N) remove 273,271 + PP_0220 0.55 -1.6 273,495 + PP_0220 0.75 -1.4 273,551 + PP_0220 0.80 +0.3 273,552 - +0.4 273,552 - PP_0220 0.81 +0.4 273,644 - PP_0220 0.89 -2.3 273,791 + +0.5 273,792 - -1.1 273,792 - -0.0 273,792 - +1.5 273,792 - +0.9 273,845 + +0.1 273,845 + +0.4 273,845 + -0.0 273,845 + +0.1 273,845 + -1.3 273,846 - -1.3 273,846 - -0.9 273,846 - -0.5 273,846 - -0.3 273,846 - -2.6 273,846 - +0.1 273,914 + PP_0221 0.18 -1.0 273,914 + PP_0221 0.18 -0.7 273,914 + PP_0221 0.18 -0.9 273,914 + PP_0221 0.18 +0.1 273,914 + PP_0221 0.18 -0.7 273,915 - PP_0221 0.18 -1.4 273,915 - PP_0221 0.18 -1.2 273,954 + PP_0221 0.23 +0.9 273,983 + PP_0221 0.27 -3.0 273,983 + PP_0221 0.27 -0.3 273,983 + PP_0221 0.27 -0.2 273,984 - PP_0221 0.27 -0.1 274,103 + PP_0221 0.42 -1.1 274,104 - PP_0221 0.42 -0.3 274,337 + PP_0221 0.71 -0.3 274,337 + PP_0221 0.71 +0.8 274,476 + PP_0221 0.88 -1.4 274,597 + -1.4 274,684 - +0.2 274,696 - +1.0 274,853 + PP_0222 0.21 -0.7 274,853 + PP_0222 0.21 +0.3 274,853 + PP_0222 0.21 +0.1 274,854 - PP_0222 0.21 -0.6 274,857 + PP_0222 0.21 +0.5 275,052 + PP_0222 0.35 -0.8 275,317 - PP_0222 0.54 +0.2 275,331 + PP_0222 0.55 -0.5 275,331 + PP_0222 0.55 +0.8 275,379 + PP_0222 0.59 -1.4 275,379 + PP_0222 0.59 -2.6 275,380 - PP_0222 0.59 -0.4 275,380 - PP_0222 0.59 -0.1 275,380 - PP_0222 0.59 +0.1 275,380 - PP_0222 0.59 +0.4 275,442 + PP_0222 0.63 +1.2 275,442 + PP_0222 0.63 +0.1 275,442 + PP_0222 0.63 -0.3 275,443 - PP_0222 0.64 -0.1 275,457 + PP_0222 0.65 +1.2
Or see this region's nucleotide sequence