Strain Fitness in Escherichia coli BW25113 around b1938

Experiment: LB 0.5x in EcoFAB_3.5mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntintG and fliE are separated by 36 nucleotidesfliE and fliF are separated by 214 nucleotidesfliF and fliG overlap by 8 nucleotidesfliG and fliH overlap by 8 nucleotides b1936: intG - predicted defective phage integrase (pseudogene) (RefSeq), at 2,010,526 to 2,010,687 intG b1937: fliE - flagellar hook-basal body protein FliE (NCBI), at 2,010,724 to 2,011,038 fliE b1938: fliF - flagellar M-ring protein (NCBI), at 2,011,253 to 2,012,911 fliF b1939: fliG - flagellar motor switch protein G (NCBI), at 2,012,904 to 2,013,899 fliG b1940: fliH - flagellar biosynthesis; export of flagellar proteins? (VIMSS), at 2,013,892 to 2,014,578 fliH Position (kb) 2011 2012 2013Strain fitness (log2 ratio) -3 -2 -1 0 1at 2010.417 kb on - strandat 2010.449 kb on - strandat 2010.511 kb on - strandat 2010.520 kb on - strandat 2010.530 kb on - strandat 2010.530 kb on - strandat 2010.547 kb on + strand, within intGat 2010.555 kb on - strand, within intGat 2010.629 kb on - strand, within intGat 2010.673 kb on + strandat 2011.024 kb on + strandat 2011.097 kb on - strandat 2011.163 kb on - strandat 2011.228 kb on + strandat 2011.236 kb on - strandat 2011.367 kb on + strandat 2011.626 kb on - strand, within fliFat 2011.626 kb on - strand, within fliFat 2011.781 kb on + strand, within fliFat 2011.856 kb on - strand, within fliFat 2012.056 kb on - strand, within fliFat 2012.086 kb on + strand, within fliFat 2012.086 kb on + strand, within fliFat 2012.086 kb on + strand, within fliFat 2012.143 kb on - strand, within fliFat 2012.143 kb on - strand, within fliFat 2012.165 kb on - strand, within fliFat 2012.208 kb on - strand, within fliFat 2012.208 kb on - strand, within fliFat 2012.276 kb on + strand, within fliFat 2012.281 kb on + strand, within fliFat 2012.281 kb on + strand, within fliFat 2012.443 kb on - strand, within fliFat 2012.523 kb on - strand, within fliFat 2012.812 kb on - strandat 2012.830 kb on + strandat 2012.865 kb on + strandat 2012.873 kb on - strandat 2012.922 kb on + strandat 2012.979 kb on + strandat 2012.979 kb on + strandat 2013.108 kb on + strand, within fliGat 2013.108 kb on + strand, within fliGat 2013.149 kb on - strand, within fliGat 2013.444 kb on + strand, within fliG

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 0.5x in EcoFAB_3.5mL
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2,010,417 - +1.0
2,010,449 - -0.4
2,010,511 - +1.0
2,010,520 - -0.6
2,010,530 - -0.3
2,010,530 - +0.1
2,010,547 + intG b1936 0.13 +1.0
2,010,555 - intG b1936 0.18 +0.7
2,010,629 - intG b1936 0.64 +1.5
2,010,673 + -3.3
2,011,024 + -0.1
2,011,097 - -1.7
2,011,163 - -0.5
2,011,228 + -0.4
2,011,236 - -0.6
2,011,367 + -0.8
2,011,626 - fliF b1938 0.22 -1.3
2,011,626 - fliF b1938 0.22 -1.9
2,011,781 + fliF b1938 0.32 -0.7
2,011,856 - fliF b1938 0.36 -0.1
2,012,056 - fliF b1938 0.48 -3.2
2,012,086 + fliF b1938 0.50 -1.0
2,012,086 + fliF b1938 0.50 -1.4
2,012,086 + fliF b1938 0.50 -0.7
2,012,143 - fliF b1938 0.54 -0.3
2,012,143 - fliF b1938 0.54 -3.0
2,012,165 - fliF b1938 0.55 -0.1
2,012,208 - fliF b1938 0.58 +0.0
2,012,208 - fliF b1938 0.58 -1.4
2,012,276 + fliF b1938 0.62 -0.6
2,012,281 + fliF b1938 0.62 -1.4
2,012,281 + fliF b1938 0.62 -0.4
2,012,443 - fliF b1938 0.72 -0.7
2,012,523 - fliF b1938 0.77 -3.1
2,012,812 - +0.3
2,012,830 + -1.2
2,012,865 + -0.9
2,012,873 - -0.8
2,012,922 + -0.7
2,012,979 + -1.5
2,012,979 + -1.5
2,013,108 + fliG b1939 0.20 -0.7
2,013,108 + fliG b1939 0.20 +0.8
2,013,149 - fliG b1939 0.25 -0.5
2,013,444 + fliG b1939 0.54 -0.2

Or see this region's nucleotide sequence