Experiment: LB in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt deoB and deoD are separated by 56 nucleotides deoD and yjjJ are separated by 166 nucleotides
b4383: deoB - phosphopentomutase (NCBI), at 4,617,626 to 4,618,849
deoB
b4384: deoD - purine nucleoside phosphorylase (NCBI), at 4,618,906 to 4,619,625
deoD
b4385: yjjJ - predicted DNA-binding transcriptional regulator (NCBI), at 4,619,792 to 4,621,123
yjjJ
Position (kb)
4618
4619
4620 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4617.961 kb on - strand, within deoB at 4618.009 kb on + strand, within deoB at 4618.009 kb on + strand, within deoB at 4618.012 kb on + strand, within deoB at 4618.012 kb on + strand, within deoB at 4618.030 kb on + strand, within deoB at 4618.030 kb on + strand, within deoB at 4618.076 kb on + strand, within deoB at 4618.088 kb on + strand, within deoB at 4618.285 kb on + strand, within deoB at 4618.335 kb on + strand, within deoB at 4618.411 kb on + strand at 4618.411 kb on + strand, within deoB at 4618.411 kb on + strand, within deoB at 4618.411 kb on + strand, within deoB at 4618.512 kb on - strand, within deoB at 4618.512 kb on - strand, within deoB at 4618.528 kb on + strand, within deoB at 4618.553 kb on - strand, within deoB at 4618.587 kb on + strand, within deoB at 4618.587 kb on + strand, within deoB at 4618.641 kb on - strand, within deoB at 4618.646 kb on + strand, within deoB at 4618.646 kb on + strand, within deoB at 4618.681 kb on + strand, within deoB at 4618.681 kb on + strand, within deoB at 4618.687 kb on + strand, within deoB at 4618.689 kb on - strand, within deoB at 4618.714 kb on + strand, within deoB at 4618.718 kb on - strand, within deoB at 4618.718 kb on - strand, within deoB at 4618.732 kb on - strand at 4618.851 kb on - strand at 4618.864 kb on + strand at 4618.872 kb on - strand at 4618.977 kb on - strand at 4618.991 kb on - strand, within deoD at 4619.050 kb on - strand, within deoD at 4619.050 kb on - strand, within deoD at 4619.089 kb on + strand, within deoD at 4619.094 kb on + strand, within deoD at 4619.094 kb on + strand, within deoD at 4619.104 kb on + strand, within deoD at 4619.104 kb on + strand, within deoD at 4619.109 kb on + strand, within deoD at 4619.117 kb on - strand, within deoD at 4619.119 kb on + strand, within deoD at 4619.119 kb on + strand, within deoD at 4619.128 kb on + strand, within deoD at 4619.131 kb on + strand, within deoD at 4619.198 kb on + strand, within deoD at 4619.223 kb on + strand, within deoD at 4619.272 kb on + strand, within deoD at 4619.279 kb on + strand, within deoD at 4619.298 kb on + strand, within deoD at 4619.298 kb on + strand, within deoD at 4619.316 kb on - strand, within deoD at 4619.335 kb on + strand, within deoD at 4619.335 kb on + strand, within deoD at 4619.336 kb on - strand, within deoD at 4619.340 kb on + strand, within deoD at 4619.355 kb on + strand, within deoD at 4619.375 kb on - strand, within deoD at 4619.375 kb on - strand, within deoD at 4619.382 kb on + strand, within deoD at 4619.382 kb on + strand, within deoD at 4619.385 kb on - strand, within deoD at 4619.385 kb on - strand, within deoD at 4619.390 kb on - strand, within deoD at 4619.390 kb on - strand, within deoD at 4619.512 kb on + strand, within deoD at 4619.513 kb on + strand, within deoD at 4619.513 kb on + strand, within deoD at 4619.513 kb on + strand, within deoD at 4619.541 kb on + strand, within deoD at 4619.564 kb on - strand at 4619.564 kb on - strand at 4619.593 kb on + strand at 4619.899 kb on + strand at 4619.899 kb on + strand at 4619.929 kb on + strand, within yjjJ at 4619.942 kb on + strand, within yjjJ at 4619.947 kb on + strand, within yjjJ at 4619.947 kb on + strand, within yjjJ at 4619.964 kb on + strand, within yjjJ at 4619.972 kb on - strand, within yjjJ at 4620.029 kb on + strand, within yjjJ at 4620.114 kb on - strand, within yjjJ at 4620.193 kb on - strand, within yjjJ at 4620.217 kb on + strand, within yjjJ at 4620.305 kb on - strand, within yjjJ at 4620.335 kb on - strand, within yjjJ at 4620.350 kb on - strand, within yjjJ at 4620.350 kb on - strand, within yjjJ at 4620.455 kb on - strand, within yjjJ at 4620.484 kb on - strand, within yjjJ at 4620.581 kb on - strand, within yjjJ at 4620.583 kb on + strand, within yjjJ at 4620.608 kb on - strand, within yjjJ
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL remove 4,617,961 - deoB b4383 0.27 +0.2 4,618,009 + deoB b4383 0.31 +0.6 4,618,009 + deoB b4383 0.31 +0.3 4,618,012 + deoB b4383 0.32 -0.2 4,618,012 + deoB b4383 0.32 +0.3 4,618,030 + deoB b4383 0.33 +0.7 4,618,030 + deoB b4383 0.33 +0.0 4,618,076 + deoB b4383 0.37 +0.3 4,618,088 + deoB b4383 0.38 +0.1 4,618,285 + deoB b4383 0.54 +0.0 4,618,335 + deoB b4383 0.58 +0.3 4,618,411 + +0.7 4,618,411 + deoB b4383 0.64 -0.3 4,618,411 + deoB b4383 0.64 -0.4 4,618,411 + deoB b4383 0.64 +0.3 4,618,512 - deoB b4383 0.72 -1.2 4,618,512 - deoB b4383 0.72 -0.9 4,618,528 + deoB b4383 0.74 -0.1 4,618,553 - deoB b4383 0.76 +0.5 4,618,587 + deoB b4383 0.79 -0.7 4,618,587 + deoB b4383 0.79 +0.7 4,618,641 - deoB b4383 0.83 -0.3 4,618,646 + deoB b4383 0.83 -0.0 4,618,646 + deoB b4383 0.83 -0.4 4,618,681 + deoB b4383 0.86 +0.6 4,618,681 + deoB b4383 0.86 -1.2 4,618,687 + deoB b4383 0.87 -1.8 4,618,689 - deoB b4383 0.87 +0.1 4,618,714 + deoB b4383 0.89 -0.0 4,618,718 - deoB b4383 0.89 +0.1 4,618,718 - deoB b4383 0.89 +0.4 4,618,732 - +1.2 4,618,851 - +0.3 4,618,864 + +0.8 4,618,872 - +0.4 4,618,977 - +1.1 4,618,991 - deoD b4384 0.12 +1.5 4,619,050 - deoD b4384 0.20 +1.7 4,619,050 - deoD b4384 0.20 +0.5 4,619,089 + deoD b4384 0.25 -1.1 4,619,094 + deoD b4384 0.26 +0.6 4,619,094 + deoD b4384 0.26 -1.0 4,619,104 + deoD b4384 0.28 +0.8 4,619,104 + deoD b4384 0.28 +0.7 4,619,109 + deoD b4384 0.28 -0.2 4,619,117 - deoD b4384 0.29 -0.4 4,619,119 + deoD b4384 0.30 +0.2 4,619,119 + deoD b4384 0.30 +0.3 4,619,128 + deoD b4384 0.31 -0.2 4,619,131 + deoD b4384 0.31 +0.1 4,619,198 + deoD b4384 0.41 -0.1 4,619,223 + deoD b4384 0.44 -0.7 4,619,272 + deoD b4384 0.51 +0.5 4,619,279 + deoD b4384 0.52 +0.4 4,619,298 + deoD b4384 0.54 +0.1 4,619,298 + deoD b4384 0.54 +1.1 4,619,316 - deoD b4384 0.57 +0.2 4,619,335 + deoD b4384 0.60 +0.7 4,619,335 + deoD b4384 0.60 +0.6 4,619,336 - deoD b4384 0.60 +0.6 4,619,340 + deoD b4384 0.60 +0.2 4,619,355 + deoD b4384 0.62 -0.1 4,619,375 - deoD b4384 0.65 -1.5 4,619,375 - deoD b4384 0.65 -0.9 4,619,382 + deoD b4384 0.66 +0.1 4,619,382 + deoD b4384 0.66 +0.7 4,619,385 - deoD b4384 0.67 -0.5 4,619,385 - deoD b4384 0.67 -0.0 4,619,390 - deoD b4384 0.67 -0.5 4,619,390 - deoD b4384 0.67 +0.9 4,619,512 + deoD b4384 0.84 +0.8 4,619,513 + deoD b4384 0.84 +0.4 4,619,513 + deoD b4384 0.84 +1.1 4,619,513 + deoD b4384 0.84 +0.2 4,619,541 + deoD b4384 0.88 +0.0 4,619,564 - +0.3 4,619,564 - -1.6 4,619,593 + +0.9 4,619,899 + -0.4 4,619,899 + -0.4 4,619,929 + yjjJ b4385 0.10 -0.6 4,619,942 + yjjJ b4385 0.11 -0.2 4,619,947 + yjjJ b4385 0.12 -0.2 4,619,947 + yjjJ b4385 0.12 -0.5 4,619,964 + yjjJ b4385 0.13 -0.2 4,619,972 - yjjJ b4385 0.14 +2.0 4,620,029 + yjjJ b4385 0.18 +0.5 4,620,114 - yjjJ b4385 0.24 +0.2 4,620,193 - yjjJ b4385 0.30 -0.0 4,620,217 + yjjJ b4385 0.32 +0.2 4,620,305 - yjjJ b4385 0.39 +0.0 4,620,335 - yjjJ b4385 0.41 -0.3 4,620,350 - yjjJ b4385 0.42 +0.4 4,620,350 - yjjJ b4385 0.42 +0.1 4,620,455 - yjjJ b4385 0.50 -0.4 4,620,484 - yjjJ b4385 0.52 -0.6 4,620,581 - yjjJ b4385 0.59 +0.1 4,620,583 + yjjJ b4385 0.59 +0.2 4,620,608 - yjjJ b4385 0.61 -0.8
Or see this region's nucleotide sequence