Strain Fitness in Escherichia coli BW25113 around b1924

Experiment: LB in EcoFAB_3.5mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfliC and fliD are separated by 265 nucleotidesfliD and fliS are separated by 24 nucleotidesfliS and fliT overlap by 1 nucleotidesfliT and amyA are separated by 77 nucleotides b1923: fliC - flagellin (NCBI), at 2,000,134 to 2,001,630 fliC b1924: fliD - flagellar capping protein (NCBI), at 2,001,896 to 2,003,302 fliD b1925: fliS - flagellar protein FliS (NCBI), at 2,003,327 to 2,003,737 fliS b1926: fliT - predicted chaperone (NCBI), at 2,003,737 to 2,004,102 fliT b1927: amyA - cytoplasmic alpha-amylase (NCBI), at 2,004,180 to 2,005,667 amyA Position (kb) 2001 2002 2003 2004Strain fitness (log2 ratio) -3 -2 -1 0 1at 2000.935 kb on + strand, within fliCat 2000.935 kb on + strand, within fliCat 2000.954 kb on + strand, within fliCat 2000.981 kb on - strand, within fliCat 2001.074 kb on - strand, within fliCat 2001.074 kb on - strand, within fliCat 2001.083 kb on - strand, within fliCat 2001.083 kb on - strand, within fliCat 2001.090 kb on - strand, within fliCat 2001.090 kb on - strand, within fliCat 2001.329 kb on + strand, within fliCat 2001.337 kb on - strand, within fliCat 2001.337 kb on - strand, within fliCat 2001.400 kb on + strand, within fliCat 2001.499 kb on - strandat 2001.522 kb on - strandat 2001.522 kb on - strandat 2001.548 kb on + strandat 2001.548 kb on + strandat 2001.706 kb on + strandat 2001.706 kb on + strandat 2001.842 kb on - strandat 2001.923 kb on + strandat 2001.964 kb on - strandat 2002.119 kb on + strand, within fliDat 2002.119 kb on + strand, within fliDat 2002.190 kb on - strand, within fliDat 2002.353 kb on + strand, within fliDat 2002.456 kb on + strand, within fliDat 2002.456 kb on + strand, within fliDat 2002.547 kb on - strand, within fliDat 2002.664 kb on - strand, within fliDat 2002.664 kb on - strand, within fliDat 2002.732 kb on - strand, within fliDat 2002.829 kb on - strand, within fliDat 2002.892 kb on - strand, within fliDat 2002.894 kb on - strand, within fliDat 2002.894 kb on - strand, within fliDat 2002.913 kb on - strand, within fliDat 2003.080 kb on - strand, within fliDat 2003.414 kb on + strand, within fliSat 2003.451 kb on - strand, within fliSat 2003.451 kb on - strand, within fliSat 2003.451 kb on - strand, within fliSat 2003.462 kb on + strand, within fliSat 2003.544 kb on + strand, within fliSat 2003.544 kb on + strand, within fliSat 2003.699 kb on + strandat 2003.734 kb on + strandat 2003.735 kb on - strandat 2003.738 kb on - strandat 2003.923 kb on - strand, within fliTat 2003.933 kb on - strand, within fliTat 2004.035 kb on + strand, within fliTat 2004.073 kb on + strandat 2004.135 kb on + strandat 2004.148 kb on + strandat 2004.241 kb on + strandat 2004.241 kb on + strandat 2004.249 kb on - strandat 2004.249 kb on - strandat 2004.249 kb on - strandat 2004.299 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL
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2,000,935 + fliC b1923 0.54 -1.4
2,000,935 + fliC b1923 0.54 -1.2
2,000,954 + fliC b1923 0.55 -1.5
2,000,981 - fliC b1923 0.57 -1.1
2,001,074 - fliC b1923 0.63 -1.8
2,001,074 - fliC b1923 0.63 -1.5
2,001,083 - fliC b1923 0.63 -0.6
2,001,083 - fliC b1923 0.63 -0.9
2,001,090 - fliC b1923 0.64 -0.5
2,001,090 - fliC b1923 0.64 -0.7
2,001,329 + fliC b1923 0.80 -0.8
2,001,337 - fliC b1923 0.80 -1.0
2,001,337 - fliC b1923 0.80 -1.1
2,001,400 + fliC b1923 0.85 -3.1
2,001,499 - -0.0
2,001,522 - +1.4
2,001,522 - +0.9
2,001,548 + -0.7
2,001,548 + -1.6
2,001,706 + +0.9
2,001,706 + -0.7
2,001,842 - -0.5
2,001,923 + +1.4
2,001,964 - -1.2
2,002,119 + fliD b1924 0.16 -0.7
2,002,119 + fliD b1924 0.16 -0.7
2,002,190 - fliD b1924 0.21 -2.0
2,002,353 + fliD b1924 0.32 -1.1
2,002,456 + fliD b1924 0.40 -1.5
2,002,456 + fliD b1924 0.40 -1.7
2,002,547 - fliD b1924 0.46 -0.5
2,002,664 - fliD b1924 0.55 -0.3
2,002,664 - fliD b1924 0.55 -1.0
2,002,732 - fliD b1924 0.59 -0.8
2,002,829 - fliD b1924 0.66 -0.4
2,002,892 - fliD b1924 0.71 -0.9
2,002,894 - fliD b1924 0.71 -0.0
2,002,894 - fliD b1924 0.71 -1.0
2,002,913 - fliD b1924 0.72 -1.6
2,003,080 - fliD b1924 0.84 -1.1
2,003,414 + fliS b1925 0.21 -1.0
2,003,451 - fliS b1925 0.30 -2.0
2,003,451 - fliS b1925 0.30 -0.4
2,003,451 - fliS b1925 0.30 -0.8
2,003,462 + fliS b1925 0.33 -1.3
2,003,544 + fliS b1925 0.53 -0.7
2,003,544 + fliS b1925 0.53 -1.5
2,003,699 + -1.8
2,003,734 + -0.0
2,003,735 - -0.8
2,003,738 - +0.8
2,003,923 - fliT b1926 0.51 -0.8
2,003,933 - fliT b1926 0.54 -0.5
2,004,035 + fliT b1926 0.81 -0.4
2,004,073 + +0.4
2,004,135 + +0.2
2,004,148 + +0.5
2,004,241 + +0.3
2,004,241 + +0.4
2,004,249 - -0.2
2,004,249 - -1.0
2,004,249 - +0.2
2,004,299 + -1.4

Or see this region's nucleotide sequence