Experiment: LB in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt flgA and flgB are separated by 154 nucleotides flgB and flgC are separated by 3 nucleotides flgC and flgD are separated by 11 nucleotides flgD and flgE are separated by 24 nucleotides
b1072: flgA - flagellar basal body P-ring biosynthesis protein A (NCBI), at 1,129,427 to 1,130,086
flgA
b1073: flgB - flagellar basal-body rod protein B (NCBI), at 1,130,241 to 1,130,657
flgB
b1074: flgC - flagellar basal-body rod protein C (NCBI), at 1,130,661 to 1,131,065
flgC
b1075: flgD - flagellar basal body rod modification protein D (NCBI), at 1,131,077 to 1,131,772
flgD
b1076: flgE - flagellar hook protein E (NCBI), at 1,131,797 to 1,133,005
flgE
Position (kb)
1131
1132 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1130.097 kb on + strand at 1130.110 kb on + strand at 1130.161 kb on + strand at 1130.236 kb on - strand at 1130.238 kb on - strand at 1130.238 kb on - strand at 1130.270 kb on - strand at 1130.352 kb on - strand, within flgB at 1130.352 kb on - strand, within flgB at 1130.411 kb on + strand, within flgB at 1130.411 kb on + strand, within flgB at 1130.456 kb on - strand, within flgB at 1130.456 kb on - strand, within flgB at 1130.513 kb on - strand, within flgB at 1130.570 kb on - strand, within flgB at 1130.693 kb on - strand at 1130.701 kb on + strand at 1131.014 kb on + strand, within flgC at 1131.014 kb on + strand, within flgC at 1131.054 kb on - strand at 1131.111 kb on + strand at 1131.325 kb on + strand, within flgD at 1131.362 kb on - strand, within flgD at 1131.362 kb on - strand, within flgD at 1131.586 kb on + strand, within flgD at 1131.594 kb on - strand, within flgD at 1131.794 kb on + strand at 1131.794 kb on + strand at 1131.846 kb on - strand at 1131.996 kb on - strand, within flgE at 1131.996 kb on - strand, within flgE at 1132.002 kb on - strand, within flgE at 1132.010 kb on + strand, within flgE at 1132.176 kb on - strand, within flgE at 1132.283 kb on - strand, within flgE at 1132.366 kb on + strand, within flgE at 1132.366 kb on + strand, within flgE at 1132.384 kb on + strand, within flgE at 1132.423 kb on + strand, within flgE at 1132.463 kb on - strand, within flgE at 1132.463 kb on - strand, within flgE at 1132.510 kb on - strand, within flgE at 1132.551 kb on + strand, within flgE at 1132.559 kb on - strand, within flgE at 1132.559 kb on - strand, within flgE at 1132.574 kb on - strand, within flgE at 1132.584 kb on - strand, within flgE at 1132.605 kb on - strand, within flgE at 1132.625 kb on - strand, within flgE at 1132.625 kb on - strand, within flgE at 1132.694 kb on - strand, within flgE
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL remove 1,130,097 + -0.4 1,130,110 + +0.2 1,130,161 + -2.4 1,130,236 - -1.6 1,130,238 - -3.4 1,130,238 - -0.8 1,130,270 - -0.9 1,130,352 - flgB b1073 0.27 -0.9 1,130,352 - flgB b1073 0.27 -2.0 1,130,411 + flgB b1073 0.41 -1.3 1,130,411 + flgB b1073 0.41 +1.6 1,130,456 - flgB b1073 0.52 -1.1 1,130,456 - flgB b1073 0.52 -0.7 1,130,513 - flgB b1073 0.65 -0.4 1,130,570 - flgB b1073 0.79 -1.6 1,130,693 - +0.7 1,130,701 + -0.7 1,131,014 + flgC b1074 0.87 -0.5 1,131,014 + flgC b1074 0.87 -0.8 1,131,054 - -1.4 1,131,111 + -0.6 1,131,325 + flgD b1075 0.36 -2.7 1,131,362 - flgD b1075 0.41 -1.1 1,131,362 - flgD b1075 0.41 -1.0 1,131,586 + flgD b1075 0.73 -0.7 1,131,594 - flgD b1075 0.74 -1.0 1,131,794 + -2.2 1,131,794 + -2.1 1,131,846 - -0.9 1,131,996 - flgE b1076 0.16 -0.6 1,131,996 - flgE b1076 0.16 -0.6 1,132,002 - flgE b1076 0.17 -1.3 1,132,010 + flgE b1076 0.18 -0.1 1,132,176 - flgE b1076 0.31 -0.6 1,132,283 - flgE b1076 0.40 -1.0 1,132,366 + flgE b1076 0.47 -0.8 1,132,366 + flgE b1076 0.47 -0.4 1,132,384 + flgE b1076 0.49 -0.9 1,132,423 + flgE b1076 0.52 -1.2 1,132,463 - flgE b1076 0.55 -1.0 1,132,463 - flgE b1076 0.55 -1.9 1,132,510 - flgE b1076 0.59 -1.2 1,132,551 + flgE b1076 0.62 -0.5 1,132,559 - flgE b1076 0.63 -0.6 1,132,559 - flgE b1076 0.63 -0.2 1,132,574 - flgE b1076 0.64 -1.5 1,132,584 - flgE b1076 0.65 -0.6 1,132,605 - flgE b1076 0.67 -2.3 1,132,625 - flgE b1076 0.68 -0.9 1,132,625 - flgE b1076 0.68 -1.1 1,132,694 - flgE b1076 0.74 -0.5
Or see this region's nucleotide sequence