Experiment: LB in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cydD and trxB are separated by 122 nucleotides trxB and lrp are separated by 544 nucleotides
b0887: cydD - fused cysteine transporter subunits of ABC superfamily: membrane component/ATP-binding component (NCBI), at 928,419 to 930,185
cydD
b0888: trxB - thioredoxin reductase, FAD/NAD(P)-binding (NCBI), at 930,308 to 931,273
trxB
b0889: lrp - DNA-binding transcriptional dual regulator, leucine-binding (NCBI), at 931,818 to 932,312
lrp
Position (kb) 930
931
932 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 930.352 kb on + strand at 930.373 kb on - strand at 930.373 kb on - strand at 930.410 kb on + strand, within trxB at 930.456 kb on - strand, within trxB at 930.458 kb on + strand, within trxB at 930.554 kb on - strand, within trxB at 930.610 kb on - strand, within trxB at 930.612 kb on + strand, within trxB at 930.634 kb on - strand at 930.848 kb on - strand, within trxB at 930.848 kb on - strand, within trxB at 930.863 kb on + strand, within trxB at 930.913 kb on - strand, within trxB at 930.939 kb on + strand, within trxB at 931.019 kb on + strand, within trxB at 931.137 kb on - strand, within trxB at 931.193 kb on - strand at 931.215 kb on - strand at 931.327 kb on - strand at 931.327 kb on - strand at 931.379 kb on + strand at 931.411 kb on + strand at 931.435 kb on + strand at 931.641 kb on - strand at 931.641 kb on - strand at 931.721 kb on - strand at 931.764 kb on - strand at 931.764 kb on - strand at 931.801 kb on + strand at 931.856 kb on - strand at 931.864 kb on + strand at 931.937 kb on + strand, within lrp at 932.002 kb on - strand, within lrp at 932.002 kb on - strand, within lrp at 932.004 kb on - strand, within lrp at 932.066 kb on - strand, within lrp at 932.078 kb on + strand, within lrp
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL remove 930,352 + -0.6 930,373 - -0.8 930,373 - -0.6 930,410 + trxB b0888 0.11 -1.3 930,456 - trxB b0888 0.15 -1.8 930,458 + trxB b0888 0.16 -1.8 930,554 - trxB b0888 0.25 -1.6 930,610 - trxB b0888 0.31 -1.2 930,612 + trxB b0888 0.31 +0.8 930,634 - -1.3 930,848 - trxB b0888 0.56 -0.2 930,848 - trxB b0888 0.56 -3.0 930,863 + trxB b0888 0.57 -1.4 930,913 - trxB b0888 0.63 -2.5 930,939 + trxB b0888 0.65 -0.9 931,019 + trxB b0888 0.74 -2.0 931,137 - trxB b0888 0.86 -0.0 931,193 - -1.0 931,215 - -1.0 931,327 - -0.0 931,327 - +0.3 931,379 + -0.1 931,411 + -1.2 931,435 + -0.5 931,641 - +1.2 931,641 - +0.2 931,721 - +1.3 931,764 - +0.9 931,764 - +0.6 931,801 + -1.1 931,856 - +0.3 931,864 + +1.3 931,937 + lrp b0889 0.24 +0.8 932,002 - lrp b0889 0.37 +1.9 932,002 - lrp b0889 0.37 +0.5 932,004 - lrp b0889 0.38 +0.6 932,066 - lrp b0889 0.50 +1.3 932,078 + lrp b0889 0.53 +1.5
Or see this region's nucleotide sequence