Strain Fitness in Pseudomonas putida KT2440 around PP_1021

Experiment: L-Leucine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_1019 and PP_1020 are separated by 117 nucleotidesPP_1020 and PP_1021 overlap by 8 nucleotidesPP_1021 and PP_1022 are separated by 211 nucleotides PP_1019: PP_1019 - carbohydrate-selective porin, at 1,162,233 to 1,163,576 _1019 PP_1020: PP_1020 - putative glucose-6-phosphate 1-epimerase, at 1,163,694 to 1,164,548 _1020 PP_1021: PP_1021 - DNA-binding transcriptional regulator, at 1,164,541 to 1,165,404 _1021 PP_1022: PP_1022 - glucose 6-phosphate-1-dehydrogenase, at 1,165,616 to 1,167,085 _1022 Position (kb) 1164 1165 1166Strain fitness (log2 ratio) -3 -2 -1 0 1at 1163.941 kb on - strand, within PP_1020at 1164.307 kb on - strand, within PP_1020at 1164.307 kb on - strand, within PP_1020at 1164.655 kb on + strand, within PP_1021at 1164.656 kb on - strand, within PP_1021at 1164.656 kb on - strand, within PP_1021at 1164.701 kb on + strand, within PP_1021at 1164.701 kb on + strand, within PP_1021at 1164.702 kb on - strand, within PP_1021at 1164.702 kb on - strand, within PP_1021at 1164.702 kb on - strand, within PP_1021at 1165.148 kb on - strand, within PP_1021at 1165.163 kb on + strand, within PP_1021at 1165.163 kb on + strand, within PP_1021at 1165.479 kb on - strandat 1165.503 kb on + strandat 1165.529 kb on + strandat 1165.552 kb on + strandat 1165.834 kb on + strand, within PP_1022at 1165.834 kb on + strand, within PP_1022at 1165.877 kb on + strand, within PP_1022at 1165.910 kb on + strand, within PP_1022at 1165.958 kb on + strand, within PP_1022at 1165.958 kb on + strand, within PP_1022at 1166.141 kb on + strand, within PP_1022at 1166.242 kb on + strand, within PP_1022

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Leucine (N)
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1,163,941 - PP_1020 0.29 -1.2
1,164,307 - PP_1020 0.72 -1.5
1,164,307 - PP_1020 0.72 -0.8
1,164,655 + PP_1021 0.13 +0.8
1,164,656 - PP_1021 0.13 +0.7
1,164,656 - PP_1021 0.13 +0.0
1,164,701 + PP_1021 0.19 +0.4
1,164,701 + PP_1021 0.19 -0.1
1,164,702 - PP_1021 0.19 +0.8
1,164,702 - PP_1021 0.19 +1.1
1,164,702 - PP_1021 0.19 -0.1
1,165,148 - PP_1021 0.70 -0.6
1,165,163 + PP_1021 0.72 +0.6
1,165,163 + PP_1021 0.72 +0.0
1,165,479 - -1.3
1,165,503 + +0.2
1,165,529 + +1.1
1,165,552 + -0.1
1,165,834 + PP_1022 0.15 -1.7
1,165,834 + PP_1022 0.15 -2.3
1,165,877 + PP_1022 0.18 -2.3
1,165,910 + PP_1022 0.20 -2.4
1,165,958 + PP_1022 0.23 -1.7
1,165,958 + PP_1022 0.23 -3.1
1,166,141 + PP_1022 0.36 -3.3
1,166,242 + PP_1022 0.43 -2.3

Or see this region's nucleotide sequence