Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2210 and PP_2211 are separated by 77 nucleotides PP_2211 and PP_2212 are separated by 146 nucleotides
PP_2210: PP_2210 - LysR family transcriptional regulator, at 2,516,640 to 2,517,503
_2210
PP_2211: PP_2211 - Transcriptional regulator, AraC family, at 2,517,581 to 2,518,603
_2211
PP_2212: PP_2212 - Leucine-rich repeat domain protein, at 2,518,750 to 2,523,195
_2212
Position (kb)
2517
2518
2519 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2516.639 kb on + strand at 2516.640 kb on - strand at 2516.656 kb on + strand at 2516.766 kb on + strand, within PP_2210 at 2516.790 kb on - strand, within PP_2210 at 2516.790 kb on - strand, within PP_2210 at 2517.283 kb on + strand, within PP_2210 at 2517.283 kb on + strand, within PP_2210 at 2517.283 kb on + strand, within PP_2210 at 2517.283 kb on + strand, within PP_2210 at 2517.499 kb on + strand at 2517.499 kb on + strand at 2517.500 kb on - strand at 2517.538 kb on + strand at 2517.538 kb on + strand at 2517.539 kb on - strand at 2517.539 kb on - strand at 2517.547 kb on + strand at 2517.547 kb on + strand at 2517.547 kb on + strand at 2517.547 kb on + strand at 2517.547 kb on + strand at 2517.548 kb on - strand at 2517.548 kb on - strand at 2517.548 kb on - strand at 2517.550 kb on + strand at 2517.550 kb on + strand at 2517.550 kb on + strand at 2517.550 kb on + strand at 2517.550 kb on + strand at 2517.735 kb on - strand, within PP_2211 at 2517.958 kb on + strand, within PP_2211 at 2517.959 kb on - strand, within PP_2211 at 2517.995 kb on + strand, within PP_2211 at 2517.996 kb on - strand, within PP_2211 at 2518.175 kb on + strand, within PP_2211 at 2518.176 kb on - strand, within PP_2211 at 2518.244 kb on - strand, within PP_2211 at 2518.256 kb on + strand, within PP_2211 at 2518.257 kb on - strand, within PP_2211 at 2518.407 kb on - strand, within PP_2211 at 2518.407 kb on - strand, within PP_2211 at 2518.428 kb on + strand, within PP_2211 at 2518.429 kb on - strand, within PP_2211 at 2518.523 kb on + strand at 2518.523 kb on + strand at 2518.523 kb on + strand at 2518.523 kb on + strand at 2518.524 kb on - strand at 2518.526 kb on - strand at 2518.526 kb on - strand at 2518.565 kb on + strand at 2518.624 kb on + strand at 2518.624 kb on + strand at 2518.730 kb on + strand at 2518.731 kb on - strand at 2518.731 kb on - strand at 2518.732 kb on + strand at 2518.732 kb on + strand at 2518.732 kb on + strand at 2518.732 kb on + strand at 2518.732 kb on + strand at 2518.732 kb on + strand at 2518.732 kb on + strand at 2518.733 kb on - strand at 2518.733 kb on - strand at 2518.733 kb on - strand at 2518.733 kb on - strand at 2518.733 kb on - strand at 2518.733 kb on - strand at 2518.733 kb on - strand at 2518.733 kb on - strand at 2518.736 kb on + strand at 2518.737 kb on - strand at 2518.737 kb on - strand at 2518.737 kb on - strand at 2518.737 kb on - strand at 2518.976 kb on + strand at 2518.976 kb on + strand at 2518.977 kb on - strand at 2519.031 kb on + strand at 2519.047 kb on + strand at 2519.047 kb on + strand at 2519.047 kb on + strand at 2519.079 kb on - strand at 2519.088 kb on + strand at 2519.116 kb on + strand at 2519.119 kb on + strand at 2519.256 kb on + strand, within PP_2212 at 2519.333 kb on + strand at 2519.333 kb on + strand, within PP_2212 at 2519.333 kb on + strand, within PP_2212 at 2519.333 kb on + strand, within PP_2212 at 2519.334 kb on - strand, within PP_2212 at 2519.506 kb on + strand, within PP_2212 at 2519.506 kb on + strand, within PP_2212
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 2,516,639 + +0.5 2,516,640 - +0.4 2,516,656 + +0.1 2,516,766 + PP_2210 0.15 +0.2 2,516,790 - PP_2210 0.17 +0.2 2,516,790 - PP_2210 0.17 -0.2 2,517,283 + PP_2210 0.74 +0.8 2,517,283 + PP_2210 0.74 -0.5 2,517,283 + PP_2210 0.74 -0.5 2,517,283 + PP_2210 0.74 -0.2 2,517,499 + +0.4 2,517,499 + +0.1 2,517,500 - +0.2 2,517,538 + +0.5 2,517,538 + +0.5 2,517,539 - -1.6 2,517,539 - -0.8 2,517,547 + -0.0 2,517,547 + -0.5 2,517,547 + -0.1 2,517,547 + -0.1 2,517,547 + -0.1 2,517,548 - -0.3 2,517,548 - -0.4 2,517,548 - -0.6 2,517,550 + +0.1 2,517,550 + +0.5 2,517,550 + +0.0 2,517,550 + +0.0 2,517,550 + +0.1 2,517,735 - PP_2211 0.15 -0.7 2,517,958 + PP_2211 0.37 -3.3 2,517,959 - PP_2211 0.37 -2.5 2,517,995 + PP_2211 0.40 -2.5 2,517,996 - PP_2211 0.41 -2.5 2,518,175 + PP_2211 0.58 -2.6 2,518,176 - PP_2211 0.58 -2.4 2,518,244 - PP_2211 0.65 -2.0 2,518,256 + PP_2211 0.66 -1.5 2,518,257 - PP_2211 0.66 -0.4 2,518,407 - PP_2211 0.81 -1.2 2,518,407 - PP_2211 0.81 -2.6 2,518,428 + PP_2211 0.83 -1.9 2,518,429 - PP_2211 0.83 -2.1 2,518,523 + -2.0 2,518,523 + -1.3 2,518,523 + -1.1 2,518,523 + -1.1 2,518,524 - -2.3 2,518,526 - -2.7 2,518,526 - -3.4 2,518,565 + -2.5 2,518,624 + -0.1 2,518,624 + +0.2 2,518,730 + +0.0 2,518,731 - -0.0 2,518,731 - +0.1 2,518,732 + -0.1 2,518,732 + +0.2 2,518,732 + +0.7 2,518,732 + -0.5 2,518,732 + -0.1 2,518,732 + +0.6 2,518,732 + -0.8 2,518,733 - -0.5 2,518,733 - -0.2 2,518,733 - -0.7 2,518,733 - -0.6 2,518,733 - -1.0 2,518,733 - -0.2 2,518,733 - +0.8 2,518,733 - +0.0 2,518,736 + -0.2 2,518,737 - +0.4 2,518,737 - +0.1 2,518,737 - +0.5 2,518,737 - -0.1 2,518,976 + +0.2 2,518,976 + -0.0 2,518,977 - +0.2 2,519,031 + +0.1 2,519,047 + +0.2 2,519,047 + -0.1 2,519,047 + -0.3 2,519,079 - -0.1 2,519,088 + -0.0 2,519,116 + +1.4 2,519,119 + +0.1 2,519,256 + PP_2212 0.11 +0.8 2,519,333 + +0.5 2,519,333 + PP_2212 0.13 -0.7 2,519,333 + PP_2212 0.13 -0.5 2,519,333 + PP_2212 0.13 +0.1 2,519,334 - PP_2212 0.13 +0.6 2,519,506 + PP_2212 0.17 -0.4 2,519,506 + PP_2212 0.17 +0.4
Or see this region's nucleotide sequence