Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0733 and PP_0734 overlap by 1 nucleotides PP_0734 and PP_0735 overlap by 7 nucleotides PP_0735 and PP_0736 overlap by 8 nucleotides PP_0736 and PP_0737 are separated by 104 nucleotides
PP_0733: PP_0733 - Peptide chain release factor 1, at 852,496 to 853,578
_0733
PP_0734: PP_0734 - release factor-(glutamine-N5) methyltransferase, at 853,578 to 854,408
_0734
PP_0735: PP_0735 - molybdopterin-synthase adenylyltransferase, at 854,402 to 855,157
_0735
PP_0736: PP_0736 - Glutamate racemase, at 855,150 to 855,947
_0736
PP_0737: PP_0737 - lipid A 3-O-deacylase, at 856,052 to 856,570
_0737
Position (kb) 854
855
856 Strain fitness (log2 ratio) -1
0
1 at 854.903 kb on + strand, within PP_0735 at 854.903 kb on + strand, within PP_0735 at 854.915 kb on + strand at 855.142 kb on + strand at 855.142 kb on + strand at 855.566 kb on - strand, within PP_0736 at 855.915 kb on - strand at 855.915 kb on - strand at 855.915 kb on - strand at 855.915 kb on - strand at 855.972 kb on + strand at 855.973 kb on - strand at 855.994 kb on - strand at 855.994 kb on - strand at 856.023 kb on + strand at 856.024 kb on - strand at 856.024 kb on - strand at 856.024 kb on - strand at 856.052 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 854,903 + PP_0735 0.66 -1.0 854,903 + PP_0735 0.66 -0.5 854,915 + -0.7 855,142 + -0.1 855,142 + +0.3 855,566 - PP_0736 0.52 -1.2 855,915 - -0.0 855,915 - -0.5 855,915 - +0.4 855,915 - +0.3 855,972 + +0.2 855,973 - -0.5 855,994 - +0.1 855,994 - +0.1 856,023 + +1.5 856,024 - -0.1 856,024 - +0.0 856,024 - +0.1 856,052 - -0.4
Or see this region's nucleotide sequence