Strain Fitness in Pseudomonas putida KT2440 around PP_0842

Experiment: L-Cysteine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0840 and PP_0841 are separated by 179 nucleotidesPP_0841 and PP_0842 are separated by 45 nucleotidesPP_0842 and PP_0843 are separated by 46 nucleotidesPP_0843 and PP_0844 are separated by 28 nucleotidesPP_0844 and PP_0845 are separated by 8 nucleotides PP_0840: PP_0840 - serine acetyltransferase, at 977,049 to 977,834 _0840 PP_0841: PP_0841 - DNA-binding transcriptional dual regulator 2Fe-2S cluster IscR, at 978,014 to 978,505 _0841 PP_0842: PP_0842 - cysteine desulfurase, at 978,551 to 979,765 _0842 PP_0843: PP_0843 - scaffold protein involved in iron-sulfur cluster assembly, at 979,812 to 980,198 _0843 PP_0844: PP_0844 - iron-sulfur cluster assembly protein, copper(I) binding, at 980,227 to 980,550 _0844 PP_0845: PP_0845 - DnaJ-like molecular chaperone specific for IscU, at 980,559 to 981,080 _0845 Position (kb) 978 979 980Strain fitness (log2 ratio) -1 0 1 2 3at 977.588 kb on + strand, within PP_0840at 977.622 kb on + strand, within PP_0840at 977.714 kb on + strand, within PP_0840at 977.715 kb on - strand, within PP_0840at 977.715 kb on - strand, within PP_0840at 977.715 kb on - strand, within PP_0840at 977.715 kb on - strand, within PP_0840at 977.748 kb on + strand, within PP_0840at 977.748 kb on + strand, within PP_0840at 977.749 kb on - strand, within PP_0840at 977.749 kb on - strand, within PP_0840at 977.749 kb on - strand, within PP_0840at 977.749 kb on - strand, within PP_0840at 978.009 kb on + strandat 978.206 kb on + strand, within PP_0841at 978.384 kb on + strand, within PP_0841at 978.384 kb on + strand, within PP_0841at 978.384 kb on + strand, within PP_0841at 978.384 kb on + strand, within PP_0841at 978.384 kb on + strand, within PP_0841at 978.539 kb on + strandat 978.539 kb on + strandat 980.196 kb on + strandat 980.215 kb on + strandat 980.215 kb on + strandat 980.215 kb on + strandat 980.564 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Cysteine (N)
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977,588 + PP_0840 0.69 +3.3
977,622 + PP_0840 0.73 +0.1
977,714 + PP_0840 0.85 +0.3
977,715 - PP_0840 0.85 +0.6
977,715 - PP_0840 0.85 +0.0
977,715 - PP_0840 0.85 +0.1
977,715 - PP_0840 0.85 +0.2
977,748 + PP_0840 0.89 -0.1
977,748 + PP_0840 0.89 +0.1
977,749 - PP_0840 0.89 +1.0
977,749 - PP_0840 0.89 +0.2
977,749 - PP_0840 0.89 -0.4
977,749 - PP_0840 0.89 +0.5
978,009 + +1.1
978,206 + PP_0841 0.39 +0.7
978,384 + PP_0841 0.75 -1.2
978,384 + PP_0841 0.75 +0.5
978,384 + PP_0841 0.75 -0.2
978,384 + PP_0841 0.75 +0.0
978,384 + PP_0841 0.75 +0.2
978,539 + -0.2
978,539 + -0.5
980,196 + -0.0
980,215 + -1.0
980,215 + -0.4
980,215 + +0.5
980,564 + -0.1

Or see this region's nucleotide sequence