Experiment: Beta-alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0081 and PP_0082 are separated by 120 nucleotides PP_0082 and PP_0083 overlap by 4 nucleotides PP_0083 and PP_0084 are separated by 110 nucleotides
PP_0081: PP_0081 - conserved protein of unknown function, at 91,078 to 91,449
_0081
PP_0082: PP_0082 - tryptophan synthase alpha chain, at 91,570 to 92,379
_0082
PP_0083: PP_0083 - tryptophan synthase beta chain, at 92,376 to 93,593
_0083
PP_0084: PP_0084 - HTH-type transcriptional regulator TrpI, at 93,704 to 94,600
_0084
Position (kb) 92
93
94 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 91.447 kb on + strand at 91.447 kb on + strand at 91.448 kb on - strand at 91.494 kb on + strand at 91.535 kb on - strand at 91.547 kb on - strand at 91.621 kb on + strand at 91.622 kb on - strand at 91.853 kb on + strand, within PP_0082 at 91.853 kb on + strand, within PP_0082 at 91.853 kb on + strand, within PP_0082 at 91.854 kb on - strand, within PP_0082 at 91.856 kb on + strand, within PP_0082 at 92.072 kb on + strand, within PP_0082 at 92.072 kb on + strand, within PP_0082 at 92.072 kb on + strand, within PP_0082 at 92.072 kb on + strand, within PP_0082 at 92.072 kb on + strand, within PP_0082 at 92.073 kb on - strand, within PP_0082 at 92.073 kb on - strand, within PP_0082 at 92.073 kb on - strand, within PP_0082 at 92.073 kb on - strand, within PP_0082 at 92.073 kb on - strand, within PP_0082 at 92.073 kb on - strand, within PP_0082 at 92.073 kb on - strand, within PP_0082 at 92.073 kb on - strand, within PP_0082 at 92.075 kb on + strand, within PP_0082 at 92.423 kb on + strand at 92.424 kb on - strand at 92.424 kb on - strand at 92.599 kb on + strand, within PP_0083 at 92.599 kb on + strand, within PP_0083 at 92.626 kb on + strand, within PP_0083 at 92.627 kb on - strand, within PP_0083 at 92.716 kb on + strand, within PP_0083 at 92.717 kb on - strand, within PP_0083 at 92.717 kb on - strand, within PP_0083 at 92.717 kb on - strand, within PP_0083 at 92.717 kb on - strand, within PP_0083 at 92.745 kb on + strand, within PP_0083 at 92.785 kb on - strand, within PP_0083 at 92.785 kb on - strand, within PP_0083 at 93.013 kb on + strand, within PP_0083 at 93.014 kb on - strand, within PP_0083 at 93.014 kb on - strand, within PP_0083 at 93.172 kb on + strand, within PP_0083 at 93.172 kb on + strand, within PP_0083 at 93.173 kb on - strand, within PP_0083 at 93.173 kb on - strand, within PP_0083 at 93.173 kb on - strand, within PP_0083 at 93.394 kb on + strand, within PP_0083 at 93.394 kb on + strand at 93.434 kb on - strand, within PP_0083 at 93.526 kb on + strand at 93.526 kb on + strand at 93.577 kb on + strand at 93.610 kb on + strand at 93.611 kb on - strand at 93.631 kb on + strand at 93.631 kb on + strand at 93.631 kb on + strand at 93.631 kb on + strand at 93.632 kb on - strand at 93.636 kb on + strand at 94.031 kb on - strand, within PP_0084 at 94.142 kb on + strand, within PP_0084 at 94.142 kb on + strand, within PP_0084 at 94.143 kb on - strand, within PP_0084 at 94.143 kb on - strand, within PP_0084 at 94.358 kb on + strand, within PP_0084 at 94.358 kb on + strand, within PP_0084 at 94.358 kb on + strand, within PP_0084 at 94.382 kb on + strand, within PP_0084 at 94.383 kb on - strand, within PP_0084 at 94.383 kb on - strand, within PP_0084 at 94.383 kb on - strand, within PP_0084 at 94.383 kb on - strand, within PP_0084 at 94.383 kb on - strand, within PP_0084 at 94.383 kb on - strand, within PP_0084 at 94.385 kb on + strand, within PP_0084 at 94.386 kb on - strand, within PP_0084 at 94.386 kb on - strand, within PP_0084 at 94.386 kb on - strand, within PP_0084 at 94.386 kb on - strand, within PP_0084 at 94.495 kb on + strand, within PP_0084 at 94.496 kb on - strand, within PP_0084
Per-strain Table
Position Strand Gene LocusTag Fraction Beta-alanine (N) remove 91,447 + -0.2 91,447 + +0.2 91,448 - +0.3 91,494 + -0.4 91,535 - -0.3 91,547 - +0.2 91,621 + -4.0 91,622 - -4.8 91,853 + PP_0082 0.35 -3.1 91,853 + PP_0082 0.35 -3.4 91,853 + PP_0082 0.35 -3.5 91,854 - PP_0082 0.35 -3.3 91,856 + PP_0082 0.35 -3.6 92,072 + PP_0082 0.62 -3.6 92,072 + PP_0082 0.62 -4.5 92,072 + PP_0082 0.62 -3.8 92,072 + PP_0082 0.62 -2.8 92,072 + PP_0082 0.62 -3.4 92,073 - PP_0082 0.62 -3.9 92,073 - PP_0082 0.62 -3.9 92,073 - PP_0082 0.62 -3.3 92,073 - PP_0082 0.62 -3.4 92,073 - PP_0082 0.62 -3.7 92,073 - PP_0082 0.62 -2.7 92,073 - PP_0082 0.62 -3.6 92,073 - PP_0082 0.62 -4.1 92,075 + PP_0082 0.62 -3.3 92,423 + -2.3 92,424 - -3.8 92,424 - -3.0 92,599 + PP_0083 0.18 -2.5 92,599 + PP_0083 0.18 -3.3 92,626 + PP_0083 0.21 -4.4 92,627 - PP_0083 0.21 -3.5 92,716 + PP_0083 0.28 -3.0 92,717 - PP_0083 0.28 -2.3 92,717 - PP_0083 0.28 -3.3 92,717 - PP_0083 0.28 -2.8 92,717 - PP_0083 0.28 -4.1 92,745 + PP_0083 0.30 -2.9 92,785 - PP_0083 0.34 -5.4 92,785 - PP_0083 0.34 -3.3 93,013 + PP_0083 0.52 -3.5 93,014 - PP_0083 0.52 -4.1 93,014 - PP_0083 0.52 -4.2 93,172 + PP_0083 0.65 -1.3 93,172 + PP_0083 0.65 -1.4 93,173 - PP_0083 0.65 -4.4 93,173 - PP_0083 0.65 -1.6 93,173 - PP_0083 0.65 -4.0 93,394 + PP_0083 0.84 -3.3 93,394 + -1.6 93,434 - PP_0083 0.87 -3.0 93,526 + -4.5 93,526 + -2.9 93,577 + -3.6 93,610 + +0.1 93,611 - -1.1 93,631 + -3.8 93,631 + -3.3 93,631 + -1.3 93,631 + -2.9 93,632 - -0.1 93,636 + -4.5 94,031 - PP_0084 0.36 +1.3 94,142 + PP_0084 0.49 +0.1 94,142 + PP_0084 0.49 +0.2 94,143 - PP_0084 0.49 +0.1 94,143 - PP_0084 0.49 -0.1 94,358 + PP_0084 0.73 -0.2 94,358 + PP_0084 0.73 -0.3 94,358 + PP_0084 0.73 -0.7 94,382 + PP_0084 0.76 -0.3 94,383 - PP_0084 0.76 -0.0 94,383 - PP_0084 0.76 +0.2 94,383 - PP_0084 0.76 +0.5 94,383 - PP_0084 0.76 -1.0 94,383 - PP_0084 0.76 +0.2 94,383 - PP_0084 0.76 -0.1 94,385 + PP_0084 0.76 -0.4 94,386 - PP_0084 0.76 -0.0 94,386 - PP_0084 0.76 -0.2 94,386 - PP_0084 0.76 -0.1 94,386 - PP_0084 0.76 +0.2 94,495 + PP_0084 0.88 -0.0 94,496 - PP_0084 0.88 -0.4
Or see this region's nucleotide sequence