Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4700 and PP_4701 are separated by 206 nucleotides PP_4701 and PP_4702 are separated by 177 nucleotides
PP_4700: PP_4700 - pantothenate synthetase, at 5,341,691 to 5,342,554
_4700
PP_4701: PP_4701 - glucose-6-phosphate isomerase, at 5,342,761 to 5,344,425
_4701
PP_4702: PP_4702 - acetyl-CoA synthetase, at 5,344,603 to 5,346,537
_4702
Position (kb)
5342
5343
5344
5345 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5341.852 kb on + strand, within PP_4700 at 5341.901 kb on + strand, within PP_4700 at 5341.902 kb on - strand, within PP_4700 at 5342.148 kb on - strand at 5342.148 kb on - strand at 5342.148 kb on - strand, within PP_4700 at 5342.148 kb on - strand at 5342.192 kb on - strand, within PP_4700 at 5342.192 kb on - strand, within PP_4700 at 5342.265 kb on - strand, within PP_4700 at 5342.284 kb on + strand, within PP_4700 at 5342.285 kb on - strand at 5342.285 kb on - strand, within PP_4700 at 5342.303 kb on + strand, within PP_4700 at 5342.304 kb on - strand, within PP_4700 at 5342.414 kb on + strand, within PP_4700 at 5342.424 kb on + strand, within PP_4700 at 5342.424 kb on + strand, within PP_4700 at 5342.425 kb on - strand at 5342.425 kb on - strand at 5342.425 kb on - strand at 5342.493 kb on - strand at 5342.526 kb on - strand at 5342.526 kb on - strand at 5342.608 kb on + strand at 5342.609 kb on - strand at 5342.609 kb on - strand at 5342.658 kb on - strand at 5342.685 kb on + strand at 5342.685 kb on + strand at 5342.685 kb on + strand at 5342.685 kb on + strand at 5342.685 kb on + strand at 5342.685 kb on + strand at 5342.686 kb on - strand at 5342.687 kb on + strand at 5342.688 kb on - strand at 5342.688 kb on - strand at 5342.688 kb on - strand at 5342.767 kb on + strand at 5342.767 kb on + strand at 5342.768 kb on - strand at 5342.768 kb on - strand at 5342.770 kb on + strand at 5342.771 kb on - strand at 5342.771 kb on - strand at 5342.775 kb on + strand at 5342.776 kb on - strand at 5342.776 kb on - strand at 5342.776 kb on - strand at 5343.442 kb on - strand, within PP_4701 at 5344.399 kb on + strand at 5344.480 kb on - strand at 5344.480 kb on - strand at 5344.487 kb on - strand at 5344.540 kb on + strand at 5344.540 kb on + strand at 5344.540 kb on + strand at 5344.540 kb on + strand at 5344.541 kb on - strand at 5344.541 kb on - strand at 5344.541 kb on - strand at 5344.551 kb on - strand at 5344.551 kb on - strand at 5344.581 kb on + strand at 5344.581 kb on + strand at 5344.582 kb on - strand at 5344.582 kb on - strand at 5344.582 kb on - strand at 5344.582 kb on - strand at 5344.622 kb on - strand at 5344.673 kb on - strand at 5344.831 kb on + strand, within PP_4702 at 5344.831 kb on + strand, within PP_4702 at 5344.831 kb on + strand, within PP_4702 at 5344.925 kb on - strand, within PP_4702 at 5345.000 kb on + strand, within PP_4702 at 5345.008 kb on + strand, within PP_4702 at 5345.008 kb on + strand, within PP_4702 at 5345.008 kb on + strand, within PP_4702 at 5345.008 kb on + strand, within PP_4702 at 5345.008 kb on + strand, within PP_4702 at 5345.009 kb on - strand, within PP_4702 at 5345.235 kb on + strand, within PP_4702 at 5345.377 kb on + strand, within PP_4702 at 5345.377 kb on + strand, within PP_4702 at 5345.377 kb on + strand, within PP_4702 at 5345.378 kb on - strand, within PP_4702 at 5345.378 kb on - strand, within PP_4702 at 5345.378 kb on - strand, within PP_4702 at 5345.418 kb on + strand, within PP_4702 at 5345.419 kb on - strand, within PP_4702 at 5345.419 kb on - strand, within PP_4702 at 5345.419 kb on - strand, within PP_4702
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 5,341,852 + PP_4700 0.19 -1.6 5,341,901 + PP_4700 0.24 -1.8 5,341,902 - PP_4700 0.24 -1.1 5,342,148 - -0.4 5,342,148 - -0.1 5,342,148 - PP_4700 0.53 +0.4 5,342,148 - -0.9 5,342,192 - PP_4700 0.58 -2.8 5,342,192 - PP_4700 0.58 -2.0 5,342,265 - PP_4700 0.66 -0.8 5,342,284 + PP_4700 0.69 -1.1 5,342,285 - +0.4 5,342,285 - PP_4700 0.69 -2.4 5,342,303 + PP_4700 0.71 -1.2 5,342,304 - PP_4700 0.71 -0.1 5,342,414 + PP_4700 0.84 +0.3 5,342,424 + PP_4700 0.85 -0.1 5,342,424 + PP_4700 0.85 -0.5 5,342,425 - -1.6 5,342,425 - +0.9 5,342,425 - -1.1 5,342,493 - -0.4 5,342,526 - +0.3 5,342,526 - -0.5 5,342,608 + -0.3 5,342,609 - +0.1 5,342,609 - -0.3 5,342,658 - -0.0 5,342,685 + -0.0 5,342,685 + -0.8 5,342,685 + +0.2 5,342,685 + +0.5 5,342,685 + -0.3 5,342,685 + -0.4 5,342,686 - -0.0 5,342,687 + -0.6 5,342,688 - +0.2 5,342,688 - +0.5 5,342,688 - -0.1 5,342,767 + +0.2 5,342,767 + -0.9 5,342,768 - -0.7 5,342,768 - +0.2 5,342,770 + +0.4 5,342,771 - -1.2 5,342,771 - -0.9 5,342,775 + -0.7 5,342,776 - -0.0 5,342,776 - -0.2 5,342,776 - -0.7 5,343,442 - PP_4701 0.41 -0.7 5,344,399 + -0.2 5,344,480 - +0.9 5,344,480 - -0.1 5,344,487 - +0.4 5,344,540 + -0.6 5,344,540 + -0.2 5,344,540 + +0.5 5,344,540 + +0.1 5,344,541 - -0.1 5,344,541 - -0.4 5,344,541 - -1.0 5,344,551 - +0.5 5,344,551 - -0.6 5,344,581 + -0.0 5,344,581 + +0.8 5,344,582 - -0.5 5,344,582 - +0.2 5,344,582 - -0.1 5,344,582 - -0.3 5,344,622 - +0.0 5,344,673 - +0.2 5,344,831 + PP_4702 0.12 +0.1 5,344,831 + PP_4702 0.12 +0.5 5,344,831 + PP_4702 0.12 -0.0 5,344,925 - PP_4702 0.17 +0.8 5,345,000 + PP_4702 0.21 -0.4 5,345,008 + PP_4702 0.21 +0.7 5,345,008 + PP_4702 0.21 -1.0 5,345,008 + PP_4702 0.21 -0.3 5,345,008 + PP_4702 0.21 -0.3 5,345,008 + PP_4702 0.21 -0.2 5,345,009 - PP_4702 0.21 +0.0 5,345,235 + PP_4702 0.33 -0.8 5,345,377 + PP_4702 0.40 -0.8 5,345,377 + PP_4702 0.40 +0.4 5,345,377 + PP_4702 0.40 -0.2 5,345,378 - PP_4702 0.40 +0.1 5,345,378 - PP_4702 0.40 -0.4 5,345,378 - PP_4702 0.40 -0.1 5,345,418 + PP_4702 0.42 -0.5 5,345,419 - PP_4702 0.42 +0.3 5,345,419 - PP_4702 0.42 +0.3 5,345,419 - PP_4702 0.42 +1.2
Or see this region's nucleotide sequence