Experiment: 2,6-diaminopimelic (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1407 and PP_t64 are separated by 210 nucleotides PP_t64 and PP_1408 are separated by 111 nucleotides
PP_1407: PP_1407 - Sulfate transporter, at 1,604,544 to 1,606,250
_1407
PP_t64: PP_t64 - tRNA-Gly, at 1,606,461 to 1,606,534
_t64
PP_1408: PP_1408 - (R)-3-hydroxydecanoyl-ACP:CoA transacylase, at 1,606,646 to 1,607,533
_1408
Position (kb)
1606
1607 Strain fitness (log2 ratio)
-2
-1
0
1 at 1605.552 kb on + strand, within PP_1407 at 1605.624 kb on + strand, within PP_1407 at 1606.047 kb on - strand, within PP_1407 at 1606.060 kb on + strand, within PP_1407 at 1606.075 kb on - strand, within PP_1407 at 1606.087 kb on + strand at 1606.087 kb on + strand at 1606.088 kb on - strand at 1606.097 kb on - strand at 1606.097 kb on - strand at 1606.097 kb on - strand at 1606.126 kb on + strand at 1606.267 kb on + strand at 1606.291 kb on - strand at 1606.291 kb on - strand at 1606.356 kb on + strand at 1606.357 kb on - strand at 1606.492 kb on + strand, within PP_t64 at 1606.493 kb on - strand, within PP_t64 at 1606.526 kb on + strand, within PP_t64 at 1606.526 kb on + strand at 1606.526 kb on + strand, within PP_t64 at 1606.527 kb on - strand at 1606.527 kb on - strand at 1606.528 kb on + strand at 1606.529 kb on - strand at 1606.585 kb on + strand at 1606.588 kb on - strand at 1606.628 kb on + strand at 1606.629 kb on - strand at 1606.687 kb on - strand at 1606.861 kb on - strand, within PP_1408 at 1606.903 kb on + strand, within PP_1408 at 1606.977 kb on + strand, within PP_1408 at 1607.010 kb on + strand, within PP_1408 at 1607.011 kb on - strand at 1607.011 kb on - strand, within PP_1408 at 1607.011 kb on - strand, within PP_1408 at 1607.100 kb on + strand, within PP_1408 at 1607.100 kb on + strand, within PP_1408 at 1607.100 kb on + strand, within PP_1408 at 1607.100 kb on + strand, within PP_1408 at 1607.122 kb on - strand, within PP_1408 at 1607.181 kb on + strand, within PP_1408 at 1607.282 kb on - strand, within PP_1408 at 1607.282 kb on - strand, within PP_1408 at 1607.346 kb on + strand, within PP_1408 at 1607.346 kb on + strand, within PP_1408 at 1607.346 kb on + strand at 1607.347 kb on - strand, within PP_1408 at 1607.389 kb on + strand, within PP_1408 at 1607.393 kb on + strand, within PP_1408 at 1607.393 kb on + strand, within PP_1408 at 1607.394 kb on - strand, within PP_1408 at 1607.472 kb on + strand at 1607.485 kb on - strand at 1607.514 kb on - strand at 1607.514 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2,6-diaminopimelic (N) remove 1,605,552 + PP_1407 0.59 +0.7 1,605,624 + PP_1407 0.63 -0.6 1,606,047 - PP_1407 0.88 -2.0 1,606,060 + PP_1407 0.89 -0.1 1,606,075 - PP_1407 0.90 -0.4 1,606,087 + +0.0 1,606,087 + -0.3 1,606,088 - -0.5 1,606,097 - -0.1 1,606,097 - -0.3 1,606,097 - +0.1 1,606,126 + -0.1 1,606,267 + +0.3 1,606,291 - +0.3 1,606,291 - +1.1 1,606,356 + +0.4 1,606,357 - -0.6 1,606,492 + PP_t64 0.42 -0.9 1,606,493 - PP_t64 0.43 +0.3 1,606,526 + PP_t64 0.88 +0.1 1,606,526 + -0.6 1,606,526 + PP_t64 0.88 -1.2 1,606,527 - -0.7 1,606,527 - -0.3 1,606,528 + -0.8 1,606,529 - -1.0 1,606,585 + +0.7 1,606,588 - +0.9 1,606,628 + -1.0 1,606,629 - +0.3 1,606,687 - -0.2 1,606,861 - PP_1408 0.24 -1.1 1,606,903 + PP_1408 0.29 -0.7 1,606,977 + PP_1408 0.37 -1.5 1,607,010 + PP_1408 0.41 -0.5 1,607,011 - -1.0 1,607,011 - PP_1408 0.41 -0.2 1,607,011 - PP_1408 0.41 -1.1 1,607,100 + PP_1408 0.51 -0.3 1,607,100 + PP_1408 0.51 -0.5 1,607,100 + PP_1408 0.51 -0.1 1,607,100 + PP_1408 0.51 -0.2 1,607,122 - PP_1408 0.54 -1.0 1,607,181 + PP_1408 0.60 -0.1 1,607,282 - PP_1408 0.72 -0.2 1,607,282 - PP_1408 0.72 -0.4 1,607,346 + PP_1408 0.79 -1.1 1,607,346 + PP_1408 0.79 -0.8 1,607,346 + -0.6 1,607,347 - PP_1408 0.79 -0.5 1,607,389 + PP_1408 0.84 -0.8 1,607,393 + PP_1408 0.84 -0.4 1,607,393 + PP_1408 0.84 -1.1 1,607,394 - PP_1408 0.84 -0.1 1,607,472 + -1.2 1,607,485 - -1.8 1,607,514 - -0.4 1,607,514 - -0.3
Or see this region's nucleotide sequence